@article { author = {Eftekhar, Maryam and Soleimanian, Saeedeh and Pourmasumi, Soheila and Ghasemi, Nasrin and Moshrefi, Mojgan}, title = {Polymorphism of Vascular Endothelial Growth Factor Gene−1154 G/A in Women with Recurrent Miscarriage and IVF Failure; A case–control study}, journal = {Journal of Kerman University of Medical Sciences}, volume = {27}, number = {2}, pages = {150-158}, year = {2020}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {10.22062/jkmu.2020.90618}, abstract = {Background: Vascular endothelial growth factor A (VEGFA) plays a key role in angiogenesis during human placenta formation and its abnormal expressions have been reported in placental tissues of women with recurrent miscarriage (RM). Objective: The aim of this study was to evaluate the correlation of polymorphism of VEGF1154 G/A gene in RM and In Vitro Fertilization (IVF) failures. Methods: The peripheral blood samples of women with RM, IVF failures and healthy women with live born children, as control group were collected. DNA samples were isolated and VEGF 1154G/A polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism. Single nucleotide polymorphism scanning was done using MnII restriction enzymes for 1154 G/A. Results: The findings of this study showed that the VEGF 1154 A/A and VEGF 1154 G/A mutation frequencies in both RM and IVF failure groups were significantly higher than those in the control group (p=0.005). The homozygous AA mutant genotype frequency in the control group was 0, While in the RM and IVF failure groups it was 30% and 13.9%, respectively (p=0.005). Moreover, the heterozygous AG genotype frequencies were higher in the RM (66.7%) and IVF failure (77.8%) compared to those in the control group (58.3%). Conclusions: It was concluded that VEGF 1154 A/A and VEGF 1154 G/A polymorphisms were associated with both RM and IVF failures. However, their relation with IVF failures was more common than RM in A/G genotype, while in A/A, the RM was higher than IVF failure.}, keywords = {Recurrent miscarriage,IVF failures,Vascular Endothelial Growth Factor,Polymorphism}, url = {https://jkmu.kmu.ac.ir/article_90618.html}, eprint = {https://jkmu.kmu.ac.ir/article_90618_489d9dc456bb30da298c3c6681966018.pdf} }