eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2006-09-01
13
2
1952
202
17580
Evaluation of the Stress Effect on Expression of BEST-5 Gene in Gultured Rat Hepatocytes and Cloning of This Gene
GH.H Hasanshahi
1
A Jafarzadeh
2
H Hakimi
3
M Rezaeian
4
R Vaziri nejhad
5
S.Z Tabatabaei
6
A Esmaeili
7
E Dikson
8
Assistant professor
Associate professor
Assistant professor
Assistant professor
Assistant professor
Instructor
Assistant professor
Professor
Introduction:Liver has important roles in body metabolic regulation and for this reason hepatocytes are used worldwide.Investigations showed that isolation of hepatocytes causes activation of stress related genes.The aim of this study was to study the stress related expression of BEST-5 following hepatocytes isolation and culture.
Method:The BEST-5 gene is cloned and analyzed for the first time from isolation and hepatocytes.very little is known about this gene and almost nothing is known about its function.RAN was isolated from hepatocytes after 3h culture and used for generation of PCR products corresponding to the BEST-5.cDNA generated was cloned into pCR2.1 plasmid vector.following transformation into TOPO10oneshot cells were grown in LB agar plates containing X-Gal and ampicillin,overnight at 37C.to confirm that the plasmids contained inserts of the correct size,the vectors obtained from mini-perparations were digested with the desired restriction enzymes.
Results:sequencing was performed for the gene.RT-PCR and Northern blotting analysis showed that Best-5 mRAN is expressed 3h after isolation and culture of primary hepatocytes (3h)BEST-5 mRNA was observed unitil 5h of culture and then there waz no detectable band of BEST-5 at further time points.Comparison of expression of the level of mRNA of BEST-5,when data statistically were analyzed showed a significant difference between the expression of BEST-5 mRNA expression at 3h with 0h,24h,35h and 48h of culture(p<0.001).
Conclusion:According to the results the stress induced by hepatocytes isolation and culture leads to the expression of Best-5 time-dependently.
https://jkmu.kmu.ac.ir/article_17580_2ebe22bf99a3c8f9f58d3dc4f2d2563f.pdf
BEST-5
Stress
hepatocytes
RT-PCR
northern Blotting
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2006-09-01
13
2
203
208
17581
The Relationship between Opium Dependency and Stroke
A Hamzeei moghadam
1
S.M.R Ahmadi mosavi
2
KH KHademi zadeh
3
Associate professor
Assistant professor
...
Introduction:Stroke is the 3 cause of death and although it is a common disease,its incidence is not the same in diffirent parts of the world and depends on various factors.opium dependency apart from being a social and cultural problem has several effects on physiological,immune and coagulation systems and conseguently the risk of stroke.
Method:The study was done in Kerman shafa hospital during 2003-2004 on 105 patients with stroke (case group) and 105 patients with urologic problems (control group).Data were collected using patients history,physical examinations and diagnostic protocols and were analyzed using Chi-Square test.both groups contained 55 females and 50 males.
Results:In the case group 31 ones (29.5%)and in the control group 18 once(10.5%) were opium dependent that shows a significant difference between the two groups(p<0.001).the relation between cigarette smoking and stroke was also determined(p<0.0001) and since both factors showed significant relation eith stroke,logistic regression analysis was done.odds ratio was 2.207(p<0.012)for cigarette smoking and 2.36(p<0.04)for opium dependency respectively.
https://jkmu.kmu.ac.ir/article_17581_89d9cf5a062860439089fa35b672d332.pdf
substance dependency
Opium
Stroke
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2006-09-01
13
2
209
214
17582
The Comparison of Total protein,Albumin and Phosphorus Level in Preeclamptic Pregnant Women and Women with Normal Pregnancy
Z Salari
1
N Eftekhari
2
M Tajodini
3
Assistant professor
Associate professor
Intern
Introduction:preeclampsia has remained one of the important obstetrical problems for which different factors have been suggested.decrease of serum levels of albumin,total protein and phosphorous have been suggested in the pathophysiology of precclampsia.the aim of this study was to determine serum levels of albumin,total protein and phosphorous in normal pregnancy and preeclamptic woman.
Method:In this case-control study,100pregnant woman aged 20 to 30 years with the gestational age of 28 to 40 weeks were studied.patients were divided into two groups:50 women as case group (preec lamsia)and 50 women as control group(normal pregnancy).the serum levels of albumin,total protein and phosphorous were measured in both groups.
Results:There were no significant difference between two groups in demographic factors and mean maternal age but duration of pregnancy was significantly difference in the two groups(36.29+3.2 weeks in case group and 38.19+2.5 in control group)(p<0.0001).the mean systolic blood pressurse and body mass index were significantly different in both groups(p<0.0001).the mean systolic blood pressure in preeclamptic women was 149.8+13.39 and in normal group was 108.7+9.73 and the mean diastolic blood pressure in preeclamptic women and in normal pregnants was 21.21+2.09.there was no significant difference between the two groups in mean serum levels of albumin,total protein and phosphorous.serum level of total protein was 5.9+1 and 6.03+0.92 and serum phosphorous was 3.53+0.68 and 3.35+0.82 in preeclamptics and normals erspectively.
Conclusions:There was no significant relationship serum between levels of albumin,total protrin and phosphorous and preeclampsia.
https://jkmu.kmu.ac.ir/article_17582_705298ccddcbae1314f221cf37a46f2d.pdf
preeclampsia
Albumin
total protein
Phosphorous
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2006-09-01
13
2
215
222
17583
The Effect of physical Activity on Spontaneous Electroencephalographic Activity in Rat
M Hoseini
1
M.R SHarifi
2
R Ataei
3
H Alaei
4
Instructor
Associatr professor
other
professor
Introduction:Exercise and physical activity are important factors for human health.It has been reported that exercise can be considerably useful in the teratment of psychological diseases.in the present study the effect of running on Spontaneous Electroencephalographic Activity (EEG) of rats was investigated.
Method:Male wistar rats weighting 190-250 gr were selected and divided into control and exercise groups.the animals of exercise group were run on treadmill(I hour a day) for duration of two weeks.Then the animals in both groups were anesthized with urethane and EEGs of them were recorded.
Result:In the exercise group alpha,beta and theta waves significantly increased and delta waves deceased in comparison to the control group.
Discussion:In the present study high frequency-low voltage waves increased and low frequency-high voltage waves decreased in the exercise group.This provides further support to the earlier supposition that increase in high frequency-low oltage waves represent promotion of consciousness.It seems that exercise could increase alertness.
https://jkmu.kmu.ac.ir/article_17583_bc79a9b55e4e3b591d3eee68fd3323cf.pdf
Exercise
waves
beta
alpha
theta
Delta
consciousness
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2006-09-01
13
2
223
229
17584
Risk Factors of Root Resorption in Orthodontic Patients Terated with Fixed Appliance
H Aghili
1
J Modaresi
2
M.H Todeh Naeim
3
Assistant professor
Assistant professor
Assistant professor
Introduction:Orthodontic treatment is associted with a risk of root resorption.The aim of this study was to determine the risk of moderate and sever root resorption in orthodontic patients treated with fixed orthodontic appliance.
Method:A case-control study was performed on 70 orthodontic treated patients with moderate and sever root resorption and 70 controls matched for age,sex,duration of treatmant,ANB index and headgear application.Data were gathered via patients records,pretreatment lateral cephalometry,pre and post treatment panoramic radiography and analyzed by SPSS 13,chi-square,Fischer exact and odds ratio tests.
Results:Seventy patients out of 140 have had root resorption.There was no difference in age,sex,headgear application,ANB difference and duretion of treatment between case and control groups.In case group,35 and 10 cases(50%-14.3%) and in control group 12 and 1 cases had respectively ahistory of asthma and allergy which was statistically significant.(p-value=0.000 and 0.005 respetively).The odds ratio for the association between moderate to sever root resorption and history of allergy was 4.83(95%C.I 2.217 to 10.526) and history of asthma was 11.494(95%C.I 1.430 to 1.956).
Conclusion:This case-control study showed an association between the history of allergy and asthma and increased risk of moderate to sever root resorption.But,in order to confirm these findings,other studies including well controlled experimental studies are needed.
https://jkmu.kmu.ac.ir/article_17584_3b6a7e09454a6880cd9748a4d1b6212d.pdf
Root resorption
Orthodontic treatment
risk factor
Asthma
Allergy
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2006-09-01
13
2
230
239
17585
Role of orFD Pseudomonas aeruginosa H103 Gene in Glucose Uptake
N Hoseini Jazani
1
A Abdi-Ali
2
E Worobec
3
Assistant professor
Assistant professor
Associate professor
Background:Pseudomonas aeruginosa is a gram negative non facultative bacterium and one of the members of normal flora in different sites of body in healthy humans.this bacterium can resist in fluids and hospital environments for a long time.Pseudomonas aeruginosa has two systems for glucose uptake:a low affinity oxidative pathway and a high affinity phosohorylative pathway.Although the role of these genes are unknown by now,they may have a role in regulation of glucose uptake.In the present study,the role of orFD gene in glucose uptake in P.aeruginosa has been investigated.
Method:orFD fragment were cloned in pUCP20 as vector and the recombinant plasmid transferred into WMA200 strain of P.aeruginose,a mutant strain of p.aeruginosa with a chromosomal deletion of orFBCD.So we compared the rats of glucose uptake by p.aeruginosa by using labeled glucose under condifions at low substrate concentration and low cell density.
Results:Carbohydrate uptake patterns differed considerably among three strains.the wild type is able to uptake glucose at a faster rate than the mutant:however,the mutant complemented with orFD shows an intermediate uptake comparing to the wild type and the mutant.
Conclusion:orFD gene has an important role in carbohydrate uptake in p.aeruhinosa strains however fuether studies are required to determine the involved mechanism.
https://jkmu.kmu.ac.ir/article_17585_d18e8a0c90e2fb1e6aa60dc96dff9c8a.pdf
aeruginosa
Glucose Pseudomonas uptake
OrFB
OrFC
OrFD
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2006-09-01
13
2
240
245
17586
Effect of Aegle Marmelos Fruit Juice Concentrate on Serum Glucose and Lipid Level and ALT/AST Acivities in Diabetic Rats
N Razmi
1
GH.A Jolodar
2
H Ebrahimi
3
H Baghshani
4
Assistant professor
Assistant professor
...
phd student
Introduction:This study was carried out to investigate the effect of bael(Aegle marmelos) on glucose,cholesterol,triglyceride,creatinine levels and the activity of ALT and AST in the serum of diabetic rats.
Method:45 Adult male sprague dawley rats were selected.the brats were divided into three groups.the first group was considered as negative CONTROL(non-diabetic rats)and diabetes mellitus was indused in the remained rats by I.V.
Results:Following induction of diabetes mellitus,concentration of serum glucose,cholesterol,triglycerde and the activity of ALT increased sigrnificantly (p<0.01),but ASTactivity and creatinine concentration not varied.Food and water intake was increased and body weight of rats was desreased (p<0.01).Consumption of bael concentrate caused a significant descraese in serum giucose,cholesterol,triglycerde and the activity of ALT (p<0.01),and also food and water intake was desreased(p<0.01).
onclusion:The results of this study show that Aegle marmelose concentrate exhibit hypoglycemic and hypolipidaemic effects in diabetic rats.
https://jkmu.kmu.ac.ir/article_17586_5bfa087ad6428d450f47b7f47bf15378.pdf
Agele marmelos
Gucose
Lipid
ALT
AST
Diabetes Mellitus
Rat
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2006-09-01
13
2
246
255
17587
Airborne Fungi Spores in Different Wards of Hospitals Affiliated to Kerman University of Medical Sciences
N Arab
1
F GHaemi
2
Fatemeh GHaemi
3
Assistant professor
Instructor
...
Introduction:The infections resulting from opportunist invasive fungi,such as Aspergillus,are an increasingly developing problem in haspitalized patients especially those suffering from immunosuppressive deficiencies.
Method:In this study,air sampling from selected wards of three hospitals affiliated to Kerman University of Medical Sciences was carried out 2 times/day over 7 months (May-October,2003) by using 500 plates containing saborodextrose-agar.
Results:According to the results,89% of the plates proved to be positive for fungal growth.Of this pool of fungi plates,1034 fungal colonies containing 16 different fungi were sorted out which in regard to the frequency were penicillium,Rhizopus,Aspergillus flavus,yeast,and Alternaria respectively.Higher rats of fungi colonies was observed in Bahonar hospital compring to the two others.the most contaminated setting were ICU wards in Bahonar and Shafa hospitals,respectively.comparison of the common wards of Bahonar and Afzalipour hosritals,revealed that the emergency room in Bahonar hospital had the highest rate of contamination.finally,among the isolated Aspergillus and fungi,Aspergillus flavus and yeast species showed the highest frequencies.
Conclusion:Considering the resultsof the present study,control of fungal contamination in hospital wards,especially those in which patients with immunosuppressive deficiency are hospitalized is highly necessary.
https://jkmu.kmu.ac.ir/article_17587_33aec4273ba24b86e8df4ae441759c5d.pdf
Airborne fungal spores
Hospital