eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
65
69
32779
Study of the Effect of MMC on the Sister Chromatid Exchange in the Human Lymphocytes
S ِAkbari-Kamranvar
1
H Mozdaranie
2
M.H Roostaie
3
Faculty Member, Department of Biology, Tabriz University, Tabriz, Iran
Professor, Department of Medical Genetics, Tarbiat Modares University
Associate Professor of Virology, Tarbiat Modares University, Tehran, Iran.
Some environmental mutagenic agents cause genomic instability and increase susceptibility of DNA damage. One of them is mitomycin C which is connected to DNA as an alkylating factor and affects susceptible cells to reduction reactions. This drug is used in chemotherapy and treatment of tumors. Study of genomic instability in the presence of different concentrations of MMC can show susceptibility of DNA damage in the patients who are under chemotherapy with this drug. For this purpose, SCE is a qualified method that shows the number of sister chromatid exchanges in the metaphasic chromosomes. The number of 105 lymphocytic cells which were separated with ficol, were cultured in media (5ml, F12 15%-20%FCS) that contains mitogen of PHA (phytohemagglutinin) and MMC in the concentrations of 3ng/ml, 6ng/ml and 9ng/ml and a control sample without MMC. The specific concentration of BrdU was added after 24 hours to cell cultures. Then metaphasic cells were halted in the metaphasic stage with colchicine after 48 hours and were stained with SCE method and were studied for the number of sister chromatid exchanges in each metaphasic plaques. Evaluation of 100 metaphasic plates showed that SCE was %3.35 in the control cells while it was %5.43, %7.1 and %8.13 in the treated cells with MMC in the concentrations of 3ng/ml, 6ng/ml and 9ng/ml. In view of the results, it is clear that MMC can cause genomic instability even in the low concentrations and it can increase SCE so that the level of SCE is become the most with the concentration of 9ng/ml and the least with the concentration of 3ng/ml. In view of relation between SCE and DNA damage, we can conclude that the genome of normal cells will be damaged in the presence of MMC and in the patients who are under chemotherapy with this drug. It means that the genome of cells will become sensitive to mutation in the presence of low concentrations of MMC. Therefore we can postulate that we should use the concentrations of less than 3 ng/ml in order to decrease mutagenic effects of MMC in normal cells.
https://jkmu.kmu.ac.ir/article_32779_5c9307fa2b19bdb0429d57f27126d0c1.pdf
MMC
SCE
cancer
Sister chromatid exchange
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
70
75
32780
Comparison of Lipids and Lipoprotein Levels of Cord Blood in Neonates in Ahwaz City with the Same Parameters in Nelson Textbook of Pediatrics
Sh Vaziri- Esfarjani
1
E Iravani
2
Assistant professor of Social Medicine
Intern, Ahwaz University of Medical Science and Health Services, Ahwaz, Iran
Adult cardiovascular disease has its roots in childhood, Various surveys have shown a significant correlation between early atherosclerotic changes indentified at autopsy of children and young adults, and both total cholesterol (TC) and low-density lipoprotein (LDL cholesterol) levels. The hypercholesterolemic neonates time for diagnosis of high risk neonate infants. The aim of this study is to compare the lipids and lipoproteins levels of cord blood with the same parameters in Nelson textbook of pediatrics. This is a cross sectional study on umbilical cord blood of 243 full term newborn infants (114 females and 129 males) who were born at the Razi Hospital of Ahwas University from March to September of 2002. For the collection of data we used the nonprobabilitic sampling. Complicated and abnormal cases were eliminated. The mean of TC, LDL-C, HDL-C and TG were: 81.02±19.75mg/dl, 48.92±16.39 mg/dl, 25.09±7.34 mg/dl and 42±29.10 mg/dl. The result showed that the mean of T.C., LDL C and TG. was significantly more than that of Nelson textbook (P=0.0001). The mean of HDL C. was significantly less than that of Nelson textbook (P=0.0001). Also the prevalence of hypercholesterolemia in neonatal infants is significantly more than that of Nelson textbook. In this study, the mean of lipids and lipoproteins was compared between males and females. The mean of TC and LDL C. in females is significantly more than male neonates (P=0.016 and P0.007). Therefore, this community is at high risk. Because the neonates with hypercholesterolemia will be more liable to face this problem in adulthood, measuring of cord blood lipids and lipoproteins is a priority in this community. Lipids profiles of parents and other 1st –degree relatives are also necessary to establish whether there is a dominantly inherited defect responsible for the hypercholoesterolemia.
https://jkmu.kmu.ac.ir/article_32780_efc5ef4b386543e36beb6dd617b7c8cc.pdf
Lipid
Lipoprotein
Hypercholestrolemia
Cord blood
neonate
Gender
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
76
84
32781
Dextrose Regimen in the Effect of Caffeine on Learning, Consolidation and Recall Phases of Memory in Mice
A.K Moghadam-Nia
1
S Yazdani
2
Associate Professor of Pharmacology and Toxicology, School of Pharmacy , Babol University of Medical Sciences and Health Services, Babol, Iran.
General Practitioner
Caffeine, dose dependently can reinforce or deteriorate learning. In previous studies, the effect of glucose on decreasing of amnesia was investigated. In this study, the effect of caffeine on three phases of learning and also probable interference of glucose in mice were examined by using of passive avoidance learning .Male albino mice were examined as follows: 1. test group: a) this group received electric shock and caffeine (25,50, 100mg/kg) without dextrose regimen, b) this group received electric shock and caffeine with dextrose regimen, 2. Control group (saline). In all groups, the latency period of moving from a cubic platform to the wire floor of aparatus in three steps was studied. Data were analysed by non- parametric Mann – Whitney U and Kruskall – Wallis H-tests. Differences between points of data were statistically considered significant at p<0.05. Caffeine in low dose (25 mg/kg) increased retention time in consolidation phase but in high dose (100mg/kg) compared to other doses, it decreased the retention time in phases of learning. According to the results, the negative effect of caffeine in high dose on all phases of memory can be reversed with long-term dextrose regimen.
https://jkmu.kmu.ac.ir/article_32781_ba09a414d2f8e0a86eac70cc7efe031d.pdf
Passive avoidance learning
Memory
Caffein
Dextrose
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
85
93
32782
The Role of Glycoconjugates in Development of Floor Plate During Early Morphogenesis in Mouse Embryo
M.M Hasanzadeh- Taheri
1
M.R Nikravesh
2
M Jalali
3
A.R Fazel
4
PhD Student of Anatomy, Faculty Member, Birjand University of Medical Sciences, Birjand, Iran.
Assosiate Professor of Anatomy
Assosiate Professor of Anatomy
Professor of Embryology and Cellular Biology, Mashhad University of Medical Sciences, Mashhad, Iran.
The floor plate is a small group of cells located at the ventral midline of neural tube. During early neurogenesis the floor plate plays critical role (s) in differentiation of ventral portion of neural tube.The purpose of this study was to determine the distribution of glycoconjugates in floor plate as well as underlying mesoderm (notochord) and their inductive activities in mouse embryos.Formaline fixed sections embedded in paraffin from 10 to 14 days old Balb/C mouse embryos were processed for histochemical studies by using five different horseradish peroxidase (HRP) labelled lectins including; Glycin max (SBA) specific for Gal and GalNac terminal sugar, Vicia Villosa (VVA) and Arachis hypogaea (peanut) for GalNac , Ulex europeus (UEA-1) and Lotus tetragonolobus (LTA) for α – L-fucose.Our results showed that SBA sensitive glycoconjugates react and change in floor plate, notochordal cells and surrounding extracellular matrix and these changes were significant (P<0.05). Extensive differences between GalNac sensitive lectins were observed during motoneuron differentiation. There was no reaction with other tested GalNac lectins. Furthermore we observed significant changes (P<0.05) in fucose glycoconjugates during notochordal development. The results suggest that the timing and distribution of SBA sensitive glycoconjugates may play a key role (s) in interactions and subsequent formation of adjacent tissues such as floor plate and notochord during critical period of morphogenesis.Our finding also showed that glycoconjugates with fucose terminal sugar may play a role (s) in notochordal development but probably have no function on floor plate development.
https://jkmu.kmu.ac.ir/article_32782_704898fa17709c0a8b6797b7aefe786d.pdf
Glycoconjugate
Notochord
Floor plate
Interaction
Embriogenesis
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
94
99
32783
A Survey on the Effect of Opium Addiction on Reducing the Age of Laryngeal Cancer in Kerman Province
M.A Damaghani
1
Assistant Professor of Otorhinolaryngology, Kerman University of Medical Sciences and Health Services, Kerman, Iran
Laryngeal cancer is a disease of old age and the most common age of presentation is 6Th. 7Th decade of life. This disease is more common in men, but male to female ratio has been decreased from 15:1 to 4:1 during the last forty years. The most common risk factors are cigarette smoking and alcohol consumption. This cross – sectional study carried out to determine the age of laryngeal cancer from 1375 to 1380. 98 patients with confirmed diagnosis of laryngeal cancer (83 men and 15 women) with the mean age of57.5±12.9 were studied. Patients were divided into two groups: addict and non –addict. Mean age of the addicted, patients was 10.5 years less than that of non- addicted. The average age of addicted patients was 55.1± 12.1 and that of non-addicted patients was 65.6± 12.8 (P=0.0001). The rate of laryngeal cancer under the age of 40 has been reported, less than 2% in international reports, but in the present study it was 6.5% and all of them were opium addicted. The number of cigarette smoked daily and length of smoking were significantly lower in addicted patients (21.5±7 cigarette daily and 25.2±11.1 years) comparing with non-addicted patients (21.5±9.5 cigarette daily and 37.4±11.9 years).Therefore the mean age of incidence in our patients is lower than that reperted in literature and it maybe due to opium consumption.
https://jkmu.kmu.ac.ir/article_32783_18f1849f310bd42db6d6c995a3c44f7d.pdf
laryngeal cancer
Opium
Addiction
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
100
108
32784
Effects of Valeriana Officinalis Extract on the Seizure Induced by Picrotoxin in Mice
M.R Heidari
1
F Razban
2
Associate Professor of Toxicology and Pharmacology, School of Pharmacy, Kerman University of Medical Sciences and Health Services, Kerman, Iran
Pharmacist
Neurological effects of Valeriana officinalis have been recognized for many years. In this study, the anticonvulsant activity of methanolic extract of the plant were investigated in mice. Picrotoxin with doses of 6 and 12.5 mg/kg were used for induction of seizures. The effects of different doses of methanolic extract of the plant on the variation of beginning time of seizures, the incidence of tonic and clonic seizures and mortality rate were determined. The beginning time of tonic seizures induced by picrotoxin (6 & 12.5 mg/kg I.P.) were delayed significantly in group which received extract (50 mg/kg I.P.) 20 min. before picrotoxin administration. Latency of beginning time of tonic seizures in mice which receive picrotoxin 6mg/kg were increased significantly from 10.58 min to 15 min (P<0.05 ) and in case of 12mg/kg of picrotoxin from 5.87 to 10.82 min.(P<0.01). The incidence of clonic seizures and mortality rate were decreased in this group of animals (P<0.05, P<0.01). The results indicated that valerian extract may be effective on generalized tonic, cloinc and tonic - clonic seizures and the dose of 50 mg/kg was enough to produce effective concentration for anticovulsive activity of the extract in mice.
https://jkmu.kmu.ac.ir/article_32784_94ab88b3462ee3f2b11aac30612bea89.pdf
Valeriana officinalis
Seizure
Picrotoxin
Mice
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
109
118
32785
Antibacterial Activity Studies of Salvia Mirzayanii and Salvia Atropatana against Six Standard Gram Positive and Gram Negative Bacteria
M.H. Moshafi
1
M Mehrabani
2
H Zolhasab
3
Assistant Professor of Microbiology
Assistant Professor of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences & Health Services, Kerman, Iran
Pharmacist
In this study antimicrobial effects of Salivia mirzayanii and Saliva atropatana were evaluated against six gram-positive and gram-negative bacteria by immersion bioautography, cylinder plate and tube dilution methods separately. In bioautography method, essential oils of Salvia mirzayanii and Salvia atropatana were separated on silicagel TLC plates by toluene-ethyl acetate (93-7). In cylander plate and tube dilution method, methanolic (80%) and aqua extracts were taken by maceration method. After concentrating the extracts, they were dried, then the 50, 25, 12.5, 6.25, 3.125 and 1.562 mg/ml distilled water solution of the dried were used for searching antimicrobial effects. Essential oil and extract of Salvia mirzayanii against E.coli, S. aureus, K. pneumonia, B. subtilis and P. aeroginosa in different dilutions were effective and essential oil and extract of Salvia atropatana against E.coli, S. aureus, S. epidermidis and B. subtilis were effective too.
https://jkmu.kmu.ac.ir/article_32785_98b6aa25cf6ebf4b427687296d7c54a0.pdf
Salvia mirzayanii
Salvia atropatana
Imersion Bioautography
Cylinder plate
Dilution tube
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
119
125
32786
The Evaluation of Pain in Neurosurgical Patients before and after Operation in Kerman Shahid Bahonar Hospital according to the McGill Pain Questionnaire
G Ebrahimi-Nejad
1
A Ebrahimi-Nejad
2
S Kohan
3
A Bahrampour
4
Assistant Professor of Clinical Psychology
Assistant Professor of Neurosurgery
Instructor of Nursing
Associate Professor of Biostatistics, Kerman University of Medical Sciences and Health Services, Kerman, Iran.
The McGill Pain Questionnaire (MPQ) consists primarily of 3 major classes of word descriptors -sensory, affective and evaluative that are used by patients to specify subjective pain experience. It also contains an intensity scale and other items to determine the properties of pain experience. The questionnaire was designed to provide quantitative measures of clinical pain that can be treated statistically. This paper describes the procedures for administration of the questionnaire and the various measures that can be derived fromِ an Iranian version in comperison with previous results which were obtained from other countries. The mean and standard deviation of these measures, based on data obtained from 46 neurosurgical patients suffering from several kinds of psychocognitive and emotional status, before and after surgery in Kerman Shahid Bahonar Hospital is presented. In addition, an experimental study utilizing the questionnaire was analyzed in order to describe the nature of the information obtained by an Iranian version. The data, taken together, indicate that the McGill Pain Questionnaire provides quantitative information that can be treated statistically, and is sufficiently sensitive to detect differences among different methods of pain relieve which work with Iranian pain patients. These results show that the dimensions are close to the previous studies conducted by Melzak and Torgerson (1971) and Ebrahimi-Nejad (1997).
https://jkmu.kmu.ac.ir/article_32786_7ffa5b6b7f854842ae7c13c3019c70b0.pdf
McGill Pain Questionnaire
Iran
Acute pain
Chronic pain
Pain theory
eng
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
2004-03-01
11
4
126
130
32787
A Case Report of Periapical Actinomycosis of Maxilla
J Fariabi
1
Sh Dabiri
2
Assistant Professor of Oral and Maxillofacial Surgery, Dentistry School
Associate Professor of Pathology, Kerman University of Medical Sciences & Health Services, Kerman, Iran
Actinomycosis is a chronic and less common infectious process that established by Actinomyces israilii. Involvement of faciocervical region is more common than thorasic and visceral types and typically involves the angle of mandible and with less occurrence rate the upper jaw may also be involved. Periapical lesions of maxilla that involved in this process by Actinomyces israilii are rare and common site of them is the periapical area of central incisors of maxilla. A subject is a 31 year old woman who after multiple effort of endodontic and surgical procedures eventually with meticolous surgical debridement and pathologic examination with diagnosis of periapical actinomycosis was treated with penicilin for 10 weeks and after that showed no sign and symptom in involved area.
https://jkmu.kmu.ac.ir/article_32787_0e82ddf0550e4ca895058b2b5e7e1c5f.pdf
Actinomycosis
Periapical infection of teeth
Infection of maxilla