Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701C-reactive protein and Platelets changes in preterm neonates with patent Ductus Arteriosus2472528951710.22062/jkmu.2019.89517ENMohammad MahdiBagheriAssistant Professor, Physiology Research Center, Kerman University of Medical Sciences, Kerman, IranHomaGhabeliAfzalipour Medical Center, Kerman University of Medical Sciences, Kerman, IranPedramNiknafsProfessor, Department of Pediatrics, Kerman University of Medical Sciences, Kerman, Iran0000-0001-7522-0700SaeedehParvareshAssistant Professor, Department of Pediatrics, Kerman University of Medical Sciences, Kerman, Iran0000-0002-0507-8910Journal Article20190701<strong>Introduction:</strong> This study aimed to investigate CRP and platelet count changes in preterm neonates with Patent Ductus Arteriosus on the seventh day of birth compared to the third day<strong>.</strong><br /> <strong>Methods:</strong> This longitudinal study was conducted on fifty neonates with PDA complications in Afzalipour hospital in 2017-2018. All neonates underwent repeated echocardiography before the closure of their arterial duct and 2 cc of blood sample was taken from each neonate and was sent to the laboratory for platelet and CRP count test.<br /> <strong>Result:</strong> The mean of platelet was 296.17±18.08 on day 7, and it was significantly different compared to day 3 (195.49 ± 10.49). The arterial ducts of 15 neonates out of 50 patients with PDA were closed on the seventh day. The mean of CRP was significantly different in neonates with PDA (35.70) compared to neonates with closed arterial ducts (2.32) on day 7.<br /> <strong>Conclusions:</strong> The results of this study revealed that PDA is associated with a low number of platelets and high level of CRP in preterm neonates.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701SNHG6 203 Transcript Could be Applied as an Auxiliary Factor for more Precise Staging of Breast Cancer2532598951810.22062/jkmu.2019.89518ENAmin JafariOliayiDepartment of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, IranMalek HosseinAsadiDepartment of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, IranFarzaneAmirmahaniGenetic division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IranJournal Article20190720<strong>Background:</strong> Nowadays long non-coding RNAs are known as interesting functional part of the transcriptome. LncRNA SNHG6 was reported to be expressed more in breast cancer tissues than non-tumor ones. As a frequent cancer among women, breast cancer treatment needs applied biomarkers for fast prognosis and diagnosis. SNHG6 RNA and its splice variants could be considered as molecular biomarkers for the breast cancer well-timed treatment.<br /> <strong>Methods:</strong> RNA extraction from 35 breast cancer tissues and their relative non-tumor tissues was done and cDNAs of the RNAs were synthesized and then RT-qPCR was performed. Relative expression of SNHG6 202 and 203 was studied in breast cancer samples.<br /> <strong>Results:</strong> The expression patterns of SNHG6 202 and 203 variants were different. Difference in the expression pattern of SNHG6 202 was significantly remarkable in relation to the HER2 status of tumor samples. SNHG6 203 was expressed in tumor and non-tumor tissues differentially and the expression difference was significant. Also, this transcript exhibited significant expression difference in different stages of the studied breast tumor samples.<br /> <strong>Conclusion:</strong> It could be stated that SNHG6 203 transcript might be considered as a prognostic and staging biomarker in breast cancer studies and treatment.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701The Protective Effect of Hydroalcoholic Extract of Green Tea (Camellia sinensis L.) on Anxiety Behaviors Induced with Chronic Noise Stress in Adult Male Wistar Rats2602708951910.22062/jkmu.2019.89519ENMohammad HosseinMohammadi Mahdiabadi HasaniDepartment of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranFatemehMasjediDepartment of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranFarnazKhadiviDepartment of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, IranShimaMohammadiDepartment of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranJournal Article20190726<strong>Background:</strong> The recruitment of medicinal herbs for the treatment of psychological disorders such as anxiety and stress has a long history. The purpose of the present study was to investigate the compensatory effects of hydroalcoholic extract of green tea on anxiety behaviors that are induced by noise stress in male Wistar rats.<br /> <strong>Methods:</strong> In this study, male Wistar rats weighing 200±20 g were randomly divided into six groups (n = 10): 1) control group, 2) sham- noise stress (two times per day and each time for 15 minutes, for 21 days, without noise stress), 3) chronic noise stress (two times per day and each time for 15 minutes, for 21 days, at 87.3 dB(A) and the frequency of 80 kHz), 4) Green tea (50, 100, 150 mg/kg i.p. for three weeks), 5) sham- green tea with saline injection for 21 days, 6) noise stress + green tea extract (50, 100, 150 mg/kg i.p. for three weeks). Their serum cortisol levels was measured by ELISA test before and after the tests. Behavioral tests were performed including elevated plus-maze, territory discrimination, and passive avoidance test. All statistical analyses were performed using SPSS software.<br /> <strong>Results:</strong> Serum corticosterone levels significantly decreased in noise stress groups treated with green tea compared to the control group (<em>p </em><0.05). Based on the passive avoidance test, the time spent in the dark box showed a significant decrease in the noise stress group treated with green tea (150 mg/kg) compared to the noise stress group. Based on the territory discrimination test, the delay for entering the foreign segment in the green tea treated-groups showed a significant decrease (p <0.05) as compared to the noise stress group. The results of the elevated plus-maze test showed a significant increase in the time spent on the open arms in green tea extract-treated groups in comparison with noise stress group.<br /> <strong>Conclusion:</strong> Findings show the unique properties of green tea extracts in reducing serum corticosterone levels and improvement of anxiety behaviors caused by noise stress in animals. In addition, the useful effects of herbal medicine on anxiety disorders were confirmed in our study.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701WRAP53 Polymorphism, rs2287498: A Case Study in Northwest of Iran?2712798952010.22062/jkmu.2019.89520ENSepehrAbdolahiDepartment of Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, IranNasserPouladiDepartment of Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran and Department of Molecular Biology and Cancer Research, Azarbaijan Shahid Madani University, Tabriz, IranJournal Article20190703<strong>Background:</strong> Non-coding RNAs apply regulations on expression or function of a gene. A class of non-coding RNAs, natural antisense transcripts, might overlap with their flanking genes and emerge a new complexity upon regulation. WRAP53, is a natural antisense transcript overlapped in a head-to-head manner on the opposite strand of TP53. It has 3 transcripts of which WRAP53β produces a protein and is needed for RNP biogenesis. Single nucleotide polymorphisms in this gene are associated with cancer susceptibility.<br /> <strong>Methods:</strong> In this study, we investigated the impact of WRAP53 Ex2+19 C>T polymorphism (rs2287498) in breast cancer susceptibility in Iranian-Azeri women, by tetra-primer amplification-refractory mutation system-polymerase chain reaction (tetra-ARMS PCR) method, in 222 patients women with breast cancer. We analyzed our data using javastat statistics package (http://statpages.org/ctab2x2.html) online software for allele and genotype frequencies and SPSS v.24 for evaluating rs2287498 association with clinicopathological features. Also, in silico experiments were carried out for predicting the second RNA structure with mfold v3.6, and for prediction of amino acid substitution effect with online software polyphen2.<br /> <strong>Results:</strong> Our results show a statistical significance of tumor size with the risk of breast cancer (p-value=0.036) but no significant genotype frequencies of rs2297498 and clinicopathological features with breast cancer susceptibility. in silico analysis estimated no significant changes in RNA or protein for this polymorphism.<br /> <strong>Conclusion:</strong> In conclusion, these findings suggest no relationship between rs2287498 and breast cancer susceptibility except with tumor size which confers a possible implication as a prognostic marker in relation to the size of the tumor.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701Comparing Fracture Resistance of Endodontically Treated Teeth Using Different Preparation-Obturation Combinations2802888952110.22062/jkmu.2019.89521ENZahra SadatMadaniAssistant Professor, Department of Endodontics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, IranAkamSaeidiAssistant Professor, Department of Endodontics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, IranAliBijaniAssistant Professor, Social Determinant of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IranHanifAllahbakhshiAssistant Professor, Department of Prosthodontics, Faculty of Dentistry, Kashan University of Medical Sciences, Kashan, IranAzadehHesarkhaniAssistant Professor, Department of Endodontics, Faculty of Dentistry, Kashan University of Medical Sciences, Kashan, IranJournal Article20190708<strong>Background:</strong>One of the main reasons for extraction of endodontically treated teeth is vertical root fracture (VRF). Some factors such as root canal treatment are involved in vertical root fracture because of their potential to weaken the tooth structure. Therefore, this study aimed to investigate the effect of combining different obturation materials and rotary systems on the fracture resistance of treated teeth.<br /> <strong>Methods:</strong>Eighty extracted maxillary central incisors were selected. The coronal portions of all teeth wereremoved at the cemento-enamel junction leaving the root segment of nearly 13 mm length. Roots were randomly divided into2 groups according to the system used in root canal preparations, Group I: ProTaper, Group II: Wave 1. Each main group was further subdivided into 2 equal subgroups according to the obturation system being used, Subgroup A: ProTaper, AH26 obturator, Subgroup B: ProTaper, Bioceramic, Subgroup C: Wave 1, AH26 obturator and Subgroup D: Wave 1, Bioceramic. Fracture resistance of each sample was measured by loading in universal testing machine. The results were analyzed with two-way ANOVA followed by multiple comparison Tukey HSD test.<br /> <strong>Results:</strong> The highest mean fracture resistance value (256.7N) was associated with Soft-Core/AH26 obturation and wave one (subgroup C), while the lowest value (239.8 N) was for Bioceramic sealer and Wave 1 (subgroup D); however, there was no statistical significant difference (P>0.05) among all tested subgroups.<br /> <strong>Conclusion:</strong> All instrumentation techniques caused weakening of the root structure and made them susceptible to fracture without any significant difference and therefore alternative strategies should be considered to reinforce endodontically treated roots.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701The Effect of Diclofenac Sodium on Nocturia Caused by Benign Prostatic Hyperplasia2892968952210.22062/jkmu.2019.89522ENMohammadrezaEbadzadehAssistant Professor, Department of Urology, Clinical Research Center, Shahid Bahonar Hospital, Kerman University of Medical Sciences, Kerman, IranRaykaSharifian AmiriMen’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranArsalanJaliliDepartment of Stem Cell and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranAfshinSarrafinejadAssistant Professor of Medical Informatics, Medical Informatics Research Center, Institute of Futures Studies in Health. Kerman University of Medical Sciences, Kerman, IranHamedZanganehMedical Practitioner, Kerman University of Medical Sciences, Kerman, IranAliassgharKetabchiAssociated Professor of Urology, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, IranJournal Article20190703<strong>Background:</strong> Nocturia is a common cause of night awakenings which bothers many patients with urinary tract diseases. NSAIDs can improve nocturia by decreasing urine production and modify the altered neural pathways between bladder and CNS. The aim of this study was to evaluate the efficacy of NSAID in the treatment of nocturia secondary to BPH. Seventy-one patients complaining of BPH with LUTS that was prominent with nocturia entered this study.<br /> <strong>Methods:</strong> Between January 2013 and March 2014, 71 men aged 50-85, complaining of BPH with LUTS that was prominent with nocturia (voiding more than 2 times at night) entered this prospective study. All patients received 100 mg Diclofenac Na suppository for 30 days at night (9pm). The main outcomes included night-time frequency; IPSS, prostate volume and PSA were recorded before and after study. Statistical analysis was performed using the paired t-test, Pearson’s correlation and Wilcoxon rank test.<br /> <strong>Results:</strong> In our study, 88.7 % of patients had an acceptable response to Diclofenac Na. Prostate size showed a significant reduction after one month treatment with diclofenac Na. There were no serious adverse effects.<br /> <strong>Conclusion:</strong> In our study Diclofenac Na can improve symptoms of BPH and can be used for the treatment of nocturia secondary to BPH.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701Buckling in the Iranian Patients with Knee Osteoarthritis: Its Frequency and Correlation with Physical Activities and Falling2973068952310.22062/jkmu.2019.89523ENAzarMoezyAssociate Professor, Sports Medicine Department, Iran University of Medical Sciences, Tehran, IranZahraAliyariM.D. Iran University of Medical Sciences, Tehran, IranSaharKavandAssistant of Sports Medicine, Sports Medicine Department, Iran University of Medical Sciences, Tehran, IranJournal Article20190811<strong>Introduction:</strong>Knee osteoarthritis (KOA), one of the most common articular diseases, sometimes leads to severe disability. This study aimed to estimate the prevalence of knee-buckling in patients with KOA during the past three months and to investigate its relation with the physical function.<br /> <strong>Methods:</strong>A cross-sectional study was performed on 190 patients with KOA. Two checklists and WOMAC questionnaire were completed by the participants. The patients’ function was evaluated by timed up and go (TUG) and six-minute walk tests (6MWT). Comparison between the two groups of patients with and without buckling was done using independent t-test and the correlations were analyzed between the variables using K square and Pearson correlation tests.<br /> <strong>Results:</strong>Eighty three patients (43.68%) had a history of buckling in the past 3 months. Knee-buckling frequency was 40.87% and 48% in women and men, respectively. Statistical analysis showed significant differences between pain (p = 0.007), TUG (p =0.0001), 6MWT (p = 0.0001), WOMAC pain (p= 0.005), WOMAC stiffness (p = 0.006) and total WOMAC scores (p=0.03) concerning the two groups with and without buckling. There was a significant correlation between buckling with gender (ρ= 0.72, p=0.025), KOA history (p=0.67, p= 0.033) and the rate of fall (ρ=0.87, p=0 .012). Moreover, there were reverse significant correlations between the history of buckling with WOMAC score (r= - 0.51, p=0.02) and the functional tests results including TUG (r= - 0.57, p=0.0001) and 6MWT <br /> (r= - 0.67, p=0.0001).<br /> <strong>Conclusion:</strong>The results indicated a prevalence of 43.68% buckling among the patients which was associated with their functional impairment.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701Postural Control and Plantar Pressure Symmetry in Male and Female Athletes With Chronic Low Back Pain When Performing Overhead Squat3073158952410.22062/jkmu.2019.89524ENBehnamGholami BorujeniPhD Candidate Department of Corrective Exercises and Sport Injury, Faculty of Physical Education and Sport Sciences, Bu Ali Sina University, Hamedan, Iran0000-0002-7404-1556AliYalfaniAssociate Professor of Sport Rehabilitation, Department of Corrective Exercises and Sport Injury, Faculty of Physical Education and Sport Sciences, Bu-Ali Sina University, Hamedan, Iran0000-0003-3696-7208Journal Article20190713<strong>Background:</strong> Low Back Pain (LBP) is one of the most prevalent problems associated with sport activities. The present study aimed to investigate the relationship between pain and plantar pressure variables. In addition, we compared these variables in male and female athletes with LBP.<br /> <strong>Methods:</strong> In this study, 47 participants with the age range of 18 to 25 years were selected (22 males and 25 females). Visual Analogue Scale (VAS) was used to evaluate the LBP severity. The plantar pressure variables were recorded using a plantar pressure measurement device (model: Foot pressing FDM-S) made by Zebris Company.<br /> <strong>Results:</strong> Length of minor axis (p <sub>male</sub>= 0.020, p <sub>female</sub>= 0.227), length of major axis <br /> (p <sub>male</sub>= 0.041, p <sub>female</sub>= 0.011), area of sway (p <sub>male</sub>= 0.0001, p <sub>female</sub>= 0.007), path length <br /> (p <sub>male</sub>= 0.053, p <sub>female</sub>= 0.001), velocity of sway (p <sub>male</sub>= 0.023, p <sub>female</sub>= 0.008), and standard deviation X (p <sub>male</sub>= 0.048, p <sub>female</sub>= 0.147) of the COP variables had a positive and significant relationship with the pain. The symmetry of plantar pressure variables did not show strong correlation with the pain intensity (p>0.05). The COP variables also showed a significant difference in area of sway (p=0.042), path length (p=0.044), and standard deviation X (p=0.043) between the males and females. Females had more oscillations than males, but there was no difference in the symmetry of plantar pressure variables between the males and females (p>0.05).<br /> <strong>Conclusion:</strong> LBP is a factor that can impair the postural control, resulting in increased risk of injury among the athletes with LBP, especially in females.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701Oral Submucous Fibrosis: a case report3163198952510.22062/jkmu.2019.89525ENMahrokhImanimoghaddamProfessor of Oral and Maxillofacial Radiology, Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran0000-0002-4705-7018AminRahpeymaProfessor of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, IranAzamsadatMadaniDepartment of Prosthodontics, Faculty of Dentistry and Dental Research Center, Mashhad University of Medical Sciences, Mashhad, IranParvanehArmanpoorDepartment of Oral and Maxillofacial Radiology, Mashhad University of Medical Sciences, Mashhad, IranParisaArmanpoorDepartment of Pediatrics, Mashhad University of Medical Sciences, IranJournal Article20190726We describe an interesting case of oral sub mucous fibrosis accompanied by severe trismus in a 23 years old man. The patient had initially been diagnosed as having internal derangement of the temporomandibular joint and conservative treatment had failed to decrease the symptoms. Despite extremely limited temporomandibular joint movement, cone beam computed tomography revealed no important abnormality. The patient had been referred to Mashhad Dental School for further evaluation. On detailed case history, the patient revealed oral adverse habits such as pan & tobacco chewing and on palpation fibrous bands were noticed on the anterior and some parts of buccal mucosa; therefore, the patient was diagnosed with oral submucous fibrosis and underwent surgical excision of fibrous bands.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951026420190701Evaluation of Medicine Prescription Pattern in Orthopedic Outpatient Clinics of Kerman Province, Iran3203298952610.22062/jkmu.2019.89526ENGholamrezaSepehriKerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran0000 0001 7999 9934ZohrehEbrahimi-MeimandPhysiology Research Center, Kerman University of Medical Sciences, Kerman, IranJournal Article20190721<strong>Background:</strong> This study was aimed to evaluate the orthopedists’ prescription pattern for outpatients referred to private clinics in Kerman province, Iran.<br /> <strong>Methods:</strong> In this study a total of 59,613 outpatients’ prescriptions issued by orthopedists contracting with Iran Health insurance organization and Iran Social Security Organization from the 1<sup>st</sup> May 2014 to the 1<sup>st</sup> May 2015 were analyzed for mean number of drugs per prescription, route of administration, drug category, drug names and the most common prescribed drugs.<br /> <strong>Results:</strong> Mean number of drugs per prescription was 2.48. Non-steroidal anti-inflammatory drugs (NSAIDs) were the most frequently prescribed drugs (60.34%) followed by vitamins & minerals (53.88%), skeletal muscle relaxants (33.18%) and corticosteroids (33.0 %). Naproxen 250mg (14.29%) and diclofenac 100mg (7.5%) were the most frequently prescribed NSAIDs. Vitamin D<sub>3 </sub>(injection & oral soft gelatin capsule) was the most frequently prescribed vitamin & mineral (24.72%). Gabapentin 300mg (12.73%) and methocarbamol 500 mg (10.59%) were the most frequently prescribed skeletal muscle relaxants. Methylprednisolone acetate (10.48%) and triamcinolone acetonide (7.45%) were the most frequently prescribed corticosteroids. Anti-ulcer drugs were prescribed only for 4.11% of outpatients.<br /> Conclusion: NSAIDs, vitamins & minerals and corticosteroids were the most frequent prescribed drugs by orthopedists in Iran. The high rate of vitamin D<sub>3</sub> and calcium prescription is a valuable finding for the prevention and treatment of osteoporosis; however, anti-ulcer drugs were prescribed lower than the requiered rate and this was a remarkable finding which increases the risk of NSAIDs /corticosteroids- induced gastropathies. Therefore, some interventions for improving drug prescription by orthopedists are suggested.