Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701Epidermal Growth Factor Receptor Expression in Oral Squamous Cell Carcinoma by Immunohistochemical Technique and its Correlation with Clinicopathological Features2832939101510.22062/jkmu.2020.91015ENZohrehDalirsaniAssociate Professor of Oral and Maxillofacial Medicine , Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran0000-0002-5898-3180BahramMemarAssociate Professor, Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, IranAtessaPakfetratProfessor of Oral and Maxillofacial Medicine, Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran0000-0003-0480-6442NooshinMohtashamProfessor of Oral and Maxillofacial pathology, Dental Research Center, Mashhad University of Medical Sciences, Mashhad, IranKazemAnvariAssociate Professor, Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, IranSaraKavehSpecialist of Cosmetic and Restorative Dentistry, Tehran, IranJournal Article20190810<strong>Background:</strong>Oral squamous cell carcinoma (OSCC) is the most common malignancy of the oral cavity. Despite some improvements in treatment, the survival rate is still very low, mainly due to the possible development of secondary malignancy or metastasis. Clinical and pathological features as well as molecular biomarkers might predict the recurrence.<br /> In recent years, many studies have been carried out on molecular biomarkers that can predict the prognosis of OSCC. One of these markers is the epidermal growth factor receptor (EGFR), which has led to different results. The aim of this study was to determine EGFR level in OSCC and to analyze its correlation with clinicopathological features.<br /> <strong>Methods:</strong> A total of 62 paraffin-embedded samples from OSCC patients treated in the oncology department of the Omid Hospital in the city of Mashhad, Iran were selected and EGFR staining was performed. The clinical and histopathological data were extracted from the medical records.<br /> <strong>Results:</strong> EGFR expression was positive in 98.4% of the cases. There was a significant difference between EGFR expression in the tumor and control cases in terms of cellularity and intensity (p˂0.001 and p=0.004, respectively). No statistically significant correlation was observed between EGFR and clinicopathological parameters. There was also no significant relationship between the cellularity and intensity expression of EGFR and patient survival (p=0.92 and p=0.42, respectively).<br /> <strong>Conclusion:</strong> In view of the high EGFR expression in squamous cell carcinoma, further studies on the role of EGFR in cell processes such as proliferation, angiogenesis and differentiation of the tumor are recommended<strong>.</strong>Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701The Impact of Cardiac Rehabilitation on Pulmonary Artery Systolic Pressure and Left Ventricular End-Diastolic Pressure in Patients after Coronary Artery Bypass Graft Surgery2943039101610.22062/jkmu.2020.91016ENMansoorMoazenzadehAssociate Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, Iran0000-0002-4391-8503KhadijehMohammadiAssistant Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, IranAfshinSarafi NejadClinical Informatics Research and Development Lab, Shafa Clinical Research Unit, Kerman University of Medical Sciences, Kerman, IranFatemehKarimi AfsharAssistant Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, IranHamidrezaRashidinejadAssociate Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, Iran0000-0002-3553-5246Journal Article20200503<strong>Background:</strong>Cardiac rehabilitation program (CRP) is a useful method of modifying cardiovascular risk factors, improving life expectancy and quality of life in patients with ischemic heart disease (IHD). The present study was conducted to evaluate the effects of cardiac rehabilitation on the pulmonary artery systolic pressure (PASP) and left ventricular end-diastolic pressure (LVEDP).<br /> <strong>Methods:</strong> This Quasi-experimental study with pretest-posttest design was conducted on 80 patients with IHD who had participated in CRP after undergoing coronary artery bypass graft (CABG) surgery. Echocardiography was performed before the beginning of CRP (the 1st session) and at the end of the rehabilitation sessions, and ventricular function indices (ejection fraction), PASP (using the tricuspid regurgitation velocity), and LVEDP (using Nagueh formula: 1.24×E/e´+1.9) were measured.<br /> <strong>Results:</strong> Ejection fraction (EF) was changed from 49.3 ± 7.8 before rehabilitation to 50.7 ± 7.4 after rehabilitation, which was a statistically significant difference (P=0.003). The pulmonary artery systolic pressure altered from 30.3 ± 8.4 before rehabilitation to 27.3 ± 6.6 after rehabilitation. The left ventricular end-diastolic pressure (LVEDP) changed from 10.5 ± 3.7 before rehabilitation to 9.1 ± 2.9 after rehabilitation, which was a statistically significant difference (P= 0.000).<br /> <strong>Conclusion:</strong> According to the results, LVEDP and PASP in patients with IHD who underwent CABGs decreased after cardiac rehabilitation.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701The Effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) Inhibition on Metabolic Flexibility during Endurance Training in Skeletal Muscles of Streptozotocin-induced Diabetic Rats3043179101710.22062/jkmu.2020.91017ENHamidMarefatiAssociate Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, IranYaserMasoumi-ArdakaniPh.D. Candidate, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, IranSaeedShakerianAssociate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran0000-0001-8717-7279AbdolhamidHabibiAssociate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, IranSoheilAminizadehAssistant Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran0000-0003-3651-3505BeydolahShahouzehiAssistant Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences & Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran0000-0002-8758-6686Journal Article20200222<strong>Background:</strong>Metabolic flexibility is the capacity of a system to adjust fuel (primarily glucose and fatty acids) oxidation based on nutrient availability. Pyruvate Dehydrogenase Kinase 4 (PDK4) is one of the main enzymes that play a critical role in metabolic flexibility. In current study, we examined PDK4 inhibition along with exercise training (ET) on the gene expression of Estrogen related-receptor alpha (ERRα), medium-chain acyl-CoA dehydrogenase (MCAD), carnitine palmitoyl transferase-<em><em>1b</em></em> (CPT-1b), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), PDK4 and citrate synthase (CS) in skeletal muscle.<br /><strong>Method:</strong>Sixty-four male Wistar rats (8 week-old) were randomly divided into 8 groups (n=8); 1- untreated control, 2- STZ-induced diabetic, 3- PDK4 inhibition, 4- endurance training (ET), 5- diabetic + PDK4 inhibition, 6- diabetic + ET, 7- PDK4 inhibition + ET, and 8- diabetic +ET + PDK4 inhibition. ERRα, MCAD, CPT-1b, PGC-1α, PDK4 and CS genes expressions were measured by Real-Time PCR and quantified by 2<sup>-ΔΔCt</sup> method.<br /><strong>Results:</strong>ERRα, MCAD, CPT-1b, PGC-1α, PDK4, and CS expressions were significantly higher in non-diabetic+ Endurance Training group compared to the control group. The expressions of CPT-1b, MCAD and CS genes were significantly lower in the non-diabetic+ endurance training/PDK4 inhibition compared to the non-diabetic+ endurance training group, and the expressions of ERRα, CPT-1b and MCAD were significantly lower in the diabetic + PDK4 inhibition group compared to the diabetic group.<br /><strong>Conclusion:</strong>In sum, PDK4 inhibition has negative effects on lipid metabolism in healthy rats, but in animals with diabetes, PDK4 inhibition can be used for improving lipid metabolism (over-expression of CS and PGC-1α).Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701A Novel in vitro Co-culture Systems on Differentiation of Embryonic Stem Cells into Oocyte-like Cells in an in vivo Manner3183289101810.22062/jkmu.2020.91018ENAliDelbariAssistant Professor, Cellular and Molecular Research Center & Department of Anatomical Sciences, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, IranMaryamNazm BojnordiAssistant Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranSinaMojaverrostamiDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, IranHatefGhasemi HamidabadiAssociate Professor, Immunogenetic Research Center & Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranZahraBagheri‑HosseinabadiAssistant Professor, Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
6- Associate Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran UniversityNourollahRezaeiAssociate Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranJournal Article20190814<strong>Background:</strong>Differentiation of Embryonic Stem Cells into Oocyte-like cells <em>in vitro</em> is challenging. Successful derivation of oocyte from stem cells can provide an alternative source for curing ovogenesis problems. The current study aims to demonstrate a new protocol with two different types of media for differentiating embryonic stem cells (ESCs) into oocyte-like cells (OLCs).<br /> <strong>Methods:</strong> After culturing mouse ESCs, embryoid bodies (EBs) were generated from ESCs by hanging drop (HD) method. To final differentiation of oocyte-like cells (OLCs), the EBs were cultured in two different types of media for 12 days (first 7 days EBs were cultured in <em>in vitro</em> maturation diluted in Granulose Cell- Conditioned Medium and Follicular Fluid [1:1:1] followed by 5 days of culture in in vitro maturation diluted in uterine condition medium [1:1] ).<br /> <strong>Results:</strong>According tothe MTT test, the viability rate increased in the experimental group compared to the control EBs cultured alone. Expression of <em>Oct4</em>, as a pluripotency marker, decreased during the differentiation process of EBs in the experimental group. Co-culturing of EBs with our mentioned protocol increased germ cell markers (<em>Stella</em> and <em>Mvh</em>) and increased Oocyte-specific markers (ZP1,<em> Fig</em>α and <em>GDF9</em>).<br /> <strong>Conclusion:</strong>Our study introduces a promising <em>in vitro</em> protocol for achieving successful oogenesis through creating interactions of EBs with granulosa cells and uterine condition medium.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701Oral Lichen Planus and Celiac Disease: is there any Relationship?3293379101910.22062/jkmu.2020.91019ENAtessaPakfetratProfessor of Oral and Maxillofacial Medicine, Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran0000-0003-0480-6442ZohrehDalirsaniAssociate Professor of Oral and Maxillofacial Medicine, Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran0000-0002-5898-3180HoomanMosannen MozaffariAssistant Professor of Gastroenterology and Hepatology, Gastroenterology and Hepatology Department, Mashhad University of Medical Sciences, Mashhad, IranMinaSheikhveysiOral and Maxillofacial Medicine Specialist, Oral and Maxillofacial Medicine Department, Faculty of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran0000-0001-7381-7394Journal Article20191114<strong>Background:</strong> Lichen planus is an autoimmune disorder and is associated with other autoimmune diseases. There is, however, little evidence of the association of oral lichen planus with celiac disease. The aim of this work was to investigate, for the first time, such an association in patients in the city of Mashhad, Iran.<br /> <strong>Methods:</strong> This case-control study was performed during October 2017 to March 2018 in the department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Mashhad University of Medical Sciences, in Iran. All participants were evaluated for Anti-TTG (IgA) and Total IgA, and in some cases for Anti-TTG IgG. Data were analyzed using SPSS software v.20.<br /> <strong>Results:</strong> A total of 96 subjects were considered in the study; 32 in the case group, and the rest in the control group. The mean value of Anti-TTG IgA was 0.12 ± 1.51 Au/ml in the oral lichen planus group, while it was 0.57 ± 1.20 Au/ml in the control group with no significant difference (P=0.167). The mean value of the Total IgA was 134.96 ± 42.86 mg/dl in the lichen planus group, and it was 129.85 ± 55.28 mg/dl in the control group, as they differ negligibly either (P=0.639). Moreover, celiac disease was not present in the population.<br /> <strong>Conclusions:</strong> We showed that there was no celiac disease present in the oral lichen planus patients as well as healthy subjects. Further studies are required to imply or to rule out the association of oral lichen planus and celiac disease.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701Effect of Troxerutin on Oxidative Stress Induced by Sciatic Nerve Ischemia-reperfusion Injury in Rats3383479102010.22062/jkmu.2020.91020ENSaidehDadpishehM.Sc. of Physiology, Department of Physiology, Iranshahr University of Medical Sciences, Iranshahr, IranHassanAhmadvandProfessor in Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranLeilaJafaripourMSc. of Anatomical Scinces, Department of Anatomy, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran and Department of Anatomical Sciences, Afzalipour Medical School, Kerman University of Medical Sciences, Kerman, Iran0000-0003-3451-0757NegarNouryazdanM.Sc. of Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranEsmaeelBabaeenezhadPh.D. Student in Clinical Biochemistry, Department of Clinical Biochemistry, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, IranHamzehShatiStudent of Medicine, Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, IranShahrokhBagheriPh.D. Student in Clinical Biochemistry, Razi Herbal Medicines Researches Center, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20190606<strong>Background:</strong>Troxerutin has antioxidant and anti-inflammatory properties and in this study, its antioxidant effect on the reduction of oxidative stress induced by ischemia-reperfusion sciatic nerve injury was investigated.<br /> <strong>Methods:</strong>In this study, 64 male rats were randomly divided into 8 groups as follows: 1- IR2: ischemia (3 hours) and reperfusion (2 days), 2- Trox+IR2: ischemia (3 hours) and reperfusion (2 days), 3- IR7: ischemia (3 hours) and reperfusion (7 days), 4- Trox+IR7: ischemia (3 hours) and reperfusion (7 days), 5- IR14: ischemia (3 hours) and reperfusion (14 days), 6- Trox+IR14: ischemia (3 hours) and reperfusion (14 days), 7- IR28: ischemia (3 hours) and reperfusion (28 days), 8- Trox+IR28: ischemia (3 hours) and reperfusion (28 days). The rats received 150 mg/kg troxerutin in one injection (single dose). After separation of serum, biochemical parameters of the serums such as NO, PON1, CAT, and GPX were measured.<br /> <strong>Results:</strong>Troxerutin significantly increased the GPX and PON1 levels in groups that their reperfusion time was 2 and 14 days (p <0.05). There was no significant difference in the levels of NO and CAT between the groups received troxerutin and control groups (P>0.05).<br /> <strong>Conclusion:</strong>Troxerutin relatively decreased the oxidative stress in the sciatic nerve ischemia-reperfusion injury by increasing the level of antioxidant enzymes.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701Effects of Boswellia serrata on Improvement of Memory Impairment in Patients with Mild Cognitive Impairment: A Double-blind, Randomized, Placebo-controlled Study3483559102110.22062/jkmu.2020.91021ENSoheilaRezakhaniAssistant Professor, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IrBehnazSedighiAssociate Professor, Neurology Research Center, Kerman University of Medical Sciences, Kerman, IranAbbasPardakhtyProfessor, Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran0000-0002-1848-5961KavehShafieeAssistant Professor, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranJournal Article20191204<strong>Background:</strong> Mild cognitive impairment (MCI) is the stage between the expected cognitive decline of normal aging and the more serious decline of dementia. In the present study, the effect of Boswellia serrata (BS) on improvement of memory impairment in patients with MCI was investigated.<br /> <strong>Methods:</strong> In this single-center randomized double-blind placebo-controlled clinical trial study, 118 patients with mild cognitive impairment (MCI) were included and randomly divided into two groups (case and control). Control group (n=59) received BS 300 mg/kg body weight twice a day and control group (n=59) received placebo for a period of three and six months. Montreal Cognitive Assessment (MoCA) test for detecting cognitive impairment was done at baseline, three and six months after the intervention.<br /> <strong>Results:</strong> A significant difference was reported in the MoCA mean score between the groups after three months (24.64 vs. 22.83) and six months of the intervention (25.22 vs. 22.7). Memory item had the greatest impact on the average final score (P≤0.0001).<br /> <strong>Conclusion:</strong> BS has a significant effect on the improvement of memory impairment in patients with MCI. Further studies are required with higher doses of BS and longer duration of treatment to assess the effects of BS on memory of patients with MCI.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701Live Birth Rate following Intrauterine Insemination in Women with Low or Very Low Level of Serum Anti-müllerian Hormone3563619102210.22062/jkmu.2020.91022ENMarziehMehrafzaObstetrician and Gynecologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran0000-0002-0476-6535TaherehZare YousefiObstetrician and Gynecologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran0000-0002-4368-6554SaharSaghati JalaliObstetrician and Gynecologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, IranAzadehRaoufiDevelopmental Biologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran0000-0002-6005-1999ElmiraHosseinzadehEmbryologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran0000-0002-5895-4937SajedehSamadniaStatistician, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran0000-0002-8981-306MalihehHabibdoostMidwife, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, IranAhmadHosseiniProfessor, Embryologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran0000-0003-1871-2957Journal Article20190912<strong>Background:</strong> While anti-Müllerian hormone (AMH) level allows quantitative evaluation of ovarian reserve, its predictive value for live births following assisted reproductive technology cycles has remained controversial. The aim of the present study was to assess the importance of AMH in predicting live birth following intrauterine insemination (IUI) in the case of low or very low ovarian reserve.<br /> <strong>Methods:</strong> In this retrospective cohort study, 123 patients with AMH≤1 ng/ml, who underwent a total of 137 IUI cycles were enrolled and evaluated for live birth rate. Patients were divided into two groups based on serum AMH levels: group 1 with low level of AMH (0.4-1 ng/ml, n=83, cycles: 95) and group 2 with very low level of AMH (≤0.4 ng/ml, n=40, cycles: 42). The results were compared between the two groups. Main outcome was the pregnancy rate.<br /> <strong>Results:</strong> The rates of biochemical pregnancy, clinical pregnancy and live birth in all patients were 11%, 8% and 7.3%, respectively. The two groups showed no significant difference in the rates of biochemical pregnancy (10.4% vs. 14.3%, p=0.3), clinical pregnancy (6.3% vs. 11.9%, p=0.2) and live birth (6.3% vs. 9.8%, p=0.5). In univariate regression analysis, baseline characteristics and ovarian stimulation parameters showed no significant relationship with the rates of pregnancy and live birth.<br /> <strong>Conclusion:</strong> In women with AMH≤1 ng/ml, serum levels of AMH did not appear to reflect pregnancy outcomes and live births following IUI. It can be concluded that in women with low or very low levels of AMH, there is chance of pregnancy, and live birth following IUI.Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951027420200701Node-First Kawasaki Disease Presented with Marked Pancarditis: a Case Report3623689102310.22062/jkmu.2020.91023ENYazdanGhandiAssociate Professor, Amirkabir Hospital, Department of Pediatrics, School of Medicine, Arak University of Medical Sciences, Arak, IranFatemehDorrehAssociate Professor, Amirkabir Hospital, Department of Pediatrics, School of Medicine, Arak University of Medical Sciences, Arak, IranRoghayehAhmadiAmirkabir Hospital, School of Medicine, Arak University of Medical Sciences, Arak, IranDanialHabibiPh.D. candidate, Department of Biostatistics, Arak University of Medical Sciences, Arak, IranJournal Article20191010Kawasaki disease is an acute inflammatory disorder of medium-sized arteries that predominantly affects cardiac coronary arteries and children under the age of 5 years. Cardiac involvement usually happens later than 10 days after the onset of illness. Most of cardiac complications are coronary artery abnormalities (ectasia or aneurysms) and subclinical myocarditis. Clinical myocarditis (symptomatic congestive heart failure), pericarditis, valvulitis and pericardial effusion, as well as pancarditis are rare.<br /> This paper reports a 5-year-old boy who had heart failure (ejection fraction 48%) in the acute stage of Kawasaki disease and pericarditis. He was admitted to the hospital following 3 days of continuous fever, bilateral cervical adenopathy and dominant right side neck of torticollis. The results of physical examination after 5 days showed typical Kawasaki disease. Cardiac examination also revealed cardiac murmur and gallop rhythm. In laboratory tests, mild liver dysfunction, hypoproteinemia and hyponatremia were discovered. During hospitalization, troponin levels were positive. The patient was treated with oral high dose aspirin (100 mg/kg/d), two doses of intravenous immunoglobulin (IVIG 2 gm/kg) and three pulses of methylprednisolone. Two weeks later, cardiac evolvements were improved without further complications. The patient exhibited dramatically clinical recovery and was healthy after 8 weeks of follow-up.<br /> This case indicates that Lymph-node-first presentation of Kawasaki disease could be examined in children with Kawasaki disease who exhibit symptoms of congestive cardiac failure, pericardial effusion and pericarditis during the acute phase of the disease.