Antibacterial and Antioxidant Characteristics of Pigments and Coelomic Fluid of Sea Urchin, Echinodermata Mathaei Species, from the Persian Gulf

Authors

1 Department of Marine Biology, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran

2 Associate Professor, Department of Marine Biology, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran

3 Associate Professor, Molecular Medicine Research Center AND Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

4 Assistant Professor, Department of Marine Biology, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran

Abstract

Background & Aims: Sea urchin immune responses are directly exposed to potentially pathogenic
microorganisms and develop defence responses mainly based on immunocytes and humoral factors
contained in the coelomic fluid. In addition, the polyhydroxylated 1, 4-naphthoquinone pigments are found
to possess excellent antimicrobial, antialgal and antioxidant activities. The present research aimed to study
the bioactive potentials (antioxidant, antibacterial and cytotoxic) of coelomic fluid and pigments shells and
spines of sea urchin, Echinodermata mathaei species.
Methods: The coelomic fluid and pigments shell and spine of sea urchin were isolated using buffered mode
and hydrogen chloride (HCl), respectively. Then, antioxidant [reducing power, DPPH radical (1, 1-diphenyl
2-picryhydrazyl) scavenging, and total antioxidant capacity), antibacterial (minimum inhibitory
concentration or MIC) and cytotoxic potentials were evaluated.
Results: The free cells of the coelomic fluid had the highest activity in the all antioxidant methods, and
the coelomocyte lysate had the highest antibacterial activity. All the differences were significant at the
level of P < 0.05.
Conclusion: The result of this research indicated that coelomic fluid and pigments shell and spine of sea
urchin, Echinodermata mathaei species, have potent antioxidant activity and the ability for scavenging
cytotoxic effects. This suggests that sea urchin shells and spines, most of which are discarded as waste after
removal of gonads, would be a new bioresource for natural antioxidants

Keywords


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