The Effect of Prolonged Exposure to Low-Frequency Electromagnetic Fields on the Cholinergic System in the Small Intestine of Male Rat


1 PhD Candidate, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 Master of Animal Physiology, Biology Department, Shiraz University, Shiraz, Iran

3 Associate Professor, Biology Department, Shiraz University, Shiraz, Iran


Background & Aims: In the recent years, increasing rate of using electronic devices which generate electromagnetic fields , has caused researchers, attention to the effects of electromagnetic fields on human health. The aim of this study is to investigate the effect of prolonged exposure to extremely low frequency electromagnetic fields(ELF) on the Cholinergic system in the small intestine of rat. Methods: A total of 21 Adult male rats were divided into three groups: experimental group which were exposed to ELF (50Hz, 1mT) for 75 days, the sham-operated group, which were kept in similar conditions, but with Off solenoid; and the control group, which were kept in normal conditions. The isolated strips of the colon were inserted into organ bath and were linked to power lab A to D system force transducer and their mechanical activity were recorded in response to different doses of acetyl choline. Data were analyzed using one way ANOVA test. Results: The relaxation of ileum in response to the acetyl choline (10-6 M) at different times and after deducting the basic tension showed a significant decrease (p≤0.05) in the experimental group as compared to the sham and control groups. While, the relaxation of ileum in response to the other dozes of acetyl choline (10-5 M) in the experimental group showed no significant difference as compared to sham and control groups. Conclusion: It can be concluded that prolonged exposure to extremely low-frequency electromagnetic fields might cause decreasing of the cholinergic receptors sensitivity.


  1. Neumann E. Digression on chemical electromagnetic field effects in membrane signal transduction-cooperativity paradigm of the acetylcholine receptor. Bioelectrochemistry 2000; 52 (1): 43-9.
  2. Aldinucci C, Carretta A, Maiorca SM, Leoncini S, Signorini C, Ciccoli L, Pessina, GP. Effect of 50 Hz electromagnetic fields on rat cortical synaptosomes. Toxicol Indust Health 2009; 25 (4-5): 249- 252.
  3. Adampourezare M, Bahaodini A. The effect of prolonged exposure to low frequency electromagnetic fields on α1 adrenergic system of isolated clon in rat. J Shahid Sadoughi Univ Med Sci 2013;20(6):724-731 [In Persian].
  4. Javadifar TS, Bahaoddini A, Ketabi MA, Gholampour F, Mirkhanni H. Effect of prolonged exposure to low-frequency electromagnetic fields on the interaction of nitrergic and cholinergic systems in the isolated rat trachea. Physiology and Pharmacology 2011; 15(3): 385-94.
  5. Dindar H, Renda N, Barlas M, Akinay A, Yazgan E, Tincer T, et al. The effect of EMF stimulation on corticosteroids- inhibited intestinal wound healing. Tokai J Exp Clin Med 1993; 18(1-2): 49-55.
  6. Kaszuba-Zwoinsko J, Gil K, Ziomber A, Zaraska W, pawlicki R, Krolczyk G, Matyja A, Thor PJ . Loss of interstitial cells of Cajal after pulsating electromagnetic field (PEMF) in gastrointestinal tract of the rats. J Physiol Pharmacol 2005; 56(3): 421-32.
  7. Keklikci U, Akpolat V, Ozekinci S, Unlu K, Celik MS. The effect of extremely low frequency magnetic field on the conjunctiva and goblet cells. Curr Eye Res 2008; 33(5): 441-6.
  8. Gruchlik AWilczok AChodurek EPolechoński WWolny DDzierzewicz Z. Effects of 300 mT static magnetic field on IL-6 secretion in normal human colon myofibroblasts. Acta Pol Pharm 2012; 69(6): 1320-4.
  9. Krantis A, Rana K, Harding RK. The effects of gamma-radiation on intestinal motor activity and faecal pellet expulsion in the guinea pig. Dig Dis Sci 1996; 41(12): 2307-16.
  10. Belousova T E, Kargina-Larenteva RA. Adrenergic nerve plexuses of heart and adrenal and myocardial catechol amines of spontaneously hypertensive rats under the influence of electromagnetic irradiation in the millimeter range. Morfologiia 1999; 115(1):16-8.
  11. Christ A, Samaras T, Klingenbock A, Kuster N. Characterization of the electromagnetic near –field absorption in layered biological tissue in the frequency range from 30 MHz to 6.000 MHz. Phys Med Biol 2006; 51(19): 4951-65.
  12. Varani K, Gessi S, Merighi S,Iannotta V, Cattabriga E,Pancaldi C, Cadossi R, Borea PA. Alternation of A(3) adenosine receptors in human neutrophils and low frequency electromagnetic fields. Biochem Pharmacol 2003; 66(10): 1897- 906
  13. Antonini R.A, Benfante R, Gotti C, Moretti M, Kuster N, Schuderer J, Clementi F, Fornasari D. Extremely low- frequency electromagnetic field (ELF- EMF) does not affect the expression of alpha3, α5 and α7 nicotinic receptor subunit genes in SH- SY5Y neuroblastoma cell line. Toxicol lett 2006; 164(3): 268- 77
  14. Masuda H, de Gannes FP, Haro E, Billaudel B, Ruffie G, Lagroye I, Veyret B. Lack of effect of 50 Hz magnetic field exposure on the binding affinity of serotonin for the 5-HT 1B receptor subtype. Brain Res 2011; 1368:44-51.
  15. Matsunaga S, Shibata O, Nishioka, K, Tsuda A, Makita T, Somikawa K . Effect of amitriptyline , a tricyclic antidepressant , on smooth muscle reactivity in isolated rat trachea. J Anesth 2009; 23(3):385- 91.
  16. Kao CH, Chu YH, Wang HW, Effects of Lidocaine on rat’s isolated tracheal smooth muscle. Eur Arch Otorhinolaryngol 2010; 267(5):817-20.
  17. Romanski KW. Does the cholinergic system modulate gastrointestinal slow waves during less active phases of migrating myoelectric complex in healthy rams. Folia Med Cracov 2003; 44(1-2):79 91.
  18. Adampourezare M, Bahaodini A. Effects of long term exposure to low frequency electromagnetic fields on the cholinergic system in rat colon. JQUMS 2014:18(2); 24-29.
  19. Krantis A, Rana K, Harding RK. The effects of gamma-radiation on intestinal motor activity and faecal pellet expulsion in the guinea pig. Dig Dis Sci 1996; 41 (12): 2307-16.
  20. Kavaliergs M, Ossenkopp KP. Calcium channel involvement in magnetic field inhibition of morphine-induced analgesia. Naunyn Schmiedebergs Arch Pharmacol 1987; 336(3): 308-15.
  21. Huang C, Ye H, Xu J, Li U, Qu A. Effect of extremely low frequency weak magnetic fields on the intracellular free calcium concentration in PC-12 tumor cells. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2000; 17 (1): 63-5.
  22. Simko M, Mattsson MO. Extremely Low frequency electromagnetic field as effectors of cellular responses in vitro: possible immune cell activation, J Cell Biochem 2004; 93(1) 83-92.