The Assosiation of Promoter Polymorphism of TGF-1 (-509C>T) with Chronic Hepatitis B in Iraninan Patients Referred to Taleghani Hospital, Tehran

Authors

1 Master of Microbiology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Professor of Virology, Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Master of Callular & Molecullar Biology, Gostroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 h.D. of Medical Virology, Gostroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5 Master of Marine Biology, Gostroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6 Associate Professor, Gostroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

7 General Practitioner Gostroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

8 Professor, Gostroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Background & Aims: Hepatitis B infection is a global health problem. Hepatitis B virus can escape from the innate immune system, however adaptive immune system mainly acts against it. Transforming growth factor (TGF-) has three isoforms in mammals. Several studies have recently shown that TGF-1 suppresses replication of hepatitis B virus. Moreover, high expression of this factor is effective in liver fibrosis. In this study, association of promoter polymorphism of TGF-1 with chronic hepatitis B was investigated. Method: In this case-control study, 109 patients with chronic hepatitis B and 109 healthy control subjects formed the study population. Genotyping was performed by PCR-RFLP method. First polymerase chain reaction (PCR) was used for amplification and then its product was digested with Eco81I enzyme. Fifteen samples were sequenced to confirm the results. Results: Genotype frequency of CC, CT, TT in patient group were respectively 19.3%, 59.6% and 21.1%. The corresponding values in the control group were respectively 30.3%, 52.3% and 17.4%. The C and T allele Frequencies in the patient group (49.1% and 50.9%) and in the control group (56.4% and 43.6%) showed no significant difference. Conclusion: There was no significant difference in genotypes of -509C>T polymorphism between control and patient groups, therefore, it can be concluded that this polymorphism is not a prognostic factor for chronic hepatitis B in Iranian patients.

Keywords