1PhD Student, Department of Medical Physics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Professor, Department of Medical Physics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3Associate Professor, Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Background and Aims: Production of acoustic cavitation by sonication has been recently recommended as
a targeted treatment. The experimental results from studies indicate that the activity of cavitation generated
by bi- or multi-frequency ultrasound irradiation is higher than that caused by single frequency irradiation. In
this study, effects dual (1 MHz and 40 kHz) and single frequency sonication on acoustic cavitation were
investigated by chemical dosimeter using iodide.
Methods: In this study, we investigated the exposure parameters in low level dual-frequency ultrasound in
near fields of 40 kHz and 1 MHz in the progressive wave mode by chemical dosimeter using iodide. Finally,
the effective protocol for enhancement of the inertial cavitation activity was determined.
Results: It has been shown that the dosimeter absorbance in the continuous mode sonication is more than the
pulse mode. Moreover, significant correlations were found between the time and intensity of single and dual
frequency sonication and the iodide dosimeter absorbance. In constant energy, inertial cavitation activity in
the pulse mode remained approximately constant. Among different combinations, the combined ultrasound
sonication in continuous mode could produce a more remarkable enhancement of the inertial cavitation
activity (1.78 times) than the algebraic sum.
Conclusion: Simultaneous combined dual frequency sonication is more effective than single frequency
sonication in producing the inertial cavitation activity.