1PhD Student, Department of Biology, School of Science, The University of Isfahan, Isfahan, Iran
2Professor, Department of Biology, School of Science, The University of Isfahan, Isfahan, Iran
Background and Aims: Gold nanoparticles have potential applications in the areas of medicine, target drug delivery, cancer diagnosis and therapy, electronic, etc. Recently, biological system is considered as an environmental friendly method for synthesis of stable nanoparticles. Methods: We demonstrated a biological system for formation of stable gold nanoparticle by using Cupriavidus necator without using additives such as stabilizing agents. The sizes and composition of nanoparticles were characterised by UV-Vis spectrophotometer, transmission electron microscopy, energy dispersive spectra, and ImageJ software. Results: Stable bio-gold nanoparticle was formed by using metalophilic bacterium Cupriavidus necator. High uniformity was observed in the presence of 1.55 mg/ml dry biomass, 1 mM Hydrogen tetrachloroaurate and 25 mM formate. Conclusions: Microbial synthesis of nanoparticle, such as gold, is an easy and eco-friendly method to preparation of stable nanoparticles.