Quinolone antibacterial agents are currently used for the treatment of various bacterial infections. The nature of functional group at the 7 position of the quinolone ring system is known to have strong influence on the spectrum and extent of in vitro antibacterial activity. Accordingly, a series of N-L2- oxo-2-(2- furyl) and N-[2- oxyimino (2- furyl) ethyl] piperazinyl quinolone derivatives were synthesized and evaluated for in vitro antibacterial activity. Compounds having 2- oxo-2-(-2 furyl) ethyl group attached to the piperazine ring were as potent as norfloxacin and ciprofloxacin. The oximes were more active than corresponding ketones and original quinolone s against gram positive bacteria but less active against gram negative bacteria. The methyl oximes and O- benzyloximes were almost a potent as the corresponding quinolones against gram positive bacteria but less active against gram negative bacteria.