To prevent the Gonococcal opthalmia neonatorum in many countries including Iran application of 1℅ tetracycline ophthalmic ointment is a common practice. To evaluate the prevalence of Gonococcal genital infection and the ophthalmic prophylaxis regimen we have tested specimens which were collected from the endocervix of 500 non – pregnant women attending a private and public gynecology clinic in Kerman. These women were at their fertility age belonged to different socioeconomic groups. According to this study the prevalence of Gonococcal genital infection was 0.4℅ . One out the 2 positive cases was Asymptomatic and the other one, Asymptomatic.