The Effects of Rosa canina Fruit Hydro alcoholic Extract on Oxidative Stress, Total Antioxidant Capacity and Haematological Parameters in Diabetic Mice

Document Type: Original Article


1 Instructor, Department of Biological Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran

2 Assistant Professor, Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran

3 Associate Professor, Biotechnology Department, East Azerbaijan Research and Education Center for Agriculture and Natural Resources, AREEO, Tabriz, Iran



Background: Diabetes is a metabolic disorder with adverse effects on haematological parameters level, oxidative stress and antioxidant defence system. This study aimed to investigate the effects of Rosa canina (RC) fruit hydro alcoholic extract on oxidative stress, total antioxidant capacity (TAC) and haematological parameters in diabetic mice.
Method: In this study, 96 mice were randomly divided into the four groups (n=24). The control and diabetic groups received normal saline (p.o., 0.2 ml). Also, RC and treatment (diabetes+RC) groups received RC hydro alcoholic extract (p.o., 500 mg/kg). Diabetes was induced by a single dose of streptozotocin (i.p., 200 mg/kg).The study parameters were evaluated on day 10, 20, and 30 after the initiation of experiments.
Results: In the second and third sampling  days, WBCs, lymphocytes, haemoglobin, RBC, MCV, MCHC, platelets, TAC and weight had a significant reduction (p<0.01) in the diabetic group in comparison to the control group. However, granulocytes, RDW, malondialdehyde (MDA) and glucose in the diabetic group significantly increased compared with the control group (p<0.01). Administration of the extract in the diabetic group significantly increased hemoglobin, MCV, MCHC, platelets, RBC, serum TAC and resulted in significant reduction in RDW and MDA levels in comparison to normal saline received diabetic animals (p<0.01).
Conclusion: Based on our results, RC fruit extract has a regulatory role in controlling oxidative stress, serum TAC and hematologic factors in mice model of diabetes.


  1. Murugesh K, Yeligar V, Dash DK, Sengupta P, Maiti BC, Maity TK. Antidiabetic, antioxidant and antihyperlipidemic status of heliotropium zeylanicum extract on streptozotocin-induced diabetes in rats. Biol Pharm Bull 2006; 29: 2202-5.
  2. Uko EK, Erhabor O, Isaac IZ, Abdulrahaman Y, Adias TC, Sani Y, et al. Some haematological parameters in patients with type-1 diabetes in Sokoto, North Western Nigeria. J Blood Lymph 2013; 3(1): 110-116.
  3. Kothari R, Bokariya P. A comparative study of haematological parameters in type i diabetes mellitus patients & healthy young adolescents. Int J Biol Med Res 2012; 3(4): 2429-32.
  4. Polo CF, Vázquez ES, Gerez EN, Caballero FA, Batlle AM. STZ-induced diabetes in mice and heme pathway enzymes. Effect of allylisopropylacetamide and alpha-tocopherol. Chem Biol Interact 1995; 95(3): 327-34.
  5. Thomas M, Tsalamandris C, MacIsaac R, Jerums G. Anaemia in diabetes: An emerging complication of microvascular disease. Curr Diabetes Rev 2005; 1(1): 107-126.
  6. Deray G, Heurtier A, Grimaldi A, Launay Vacher V, Isnard Bagnis C. Anemia and diabetes. Am J Nephrol 2004; 24(5): 522-6.
  7. Askarishahi M, Hajizadeh E, Afkhami-Ardakani M. Factors affecting retinopathy in patients with type 2 diabetes by analyzing the current status data. Tehran Uni Med J 2011; 68(11): 674-80.
  8. Alba-Loureiro TC, Hirabara SM, Mendonca JR, Curi R, Pithon-Curi TC. Diabetes causes marked changes in function and metabolism of rat neutrophils. J Endocrinol 2006; 188(2): 295-303.
  9. Papanas N, Symeonidis G, Maltezos E, Mavridis G, Karavageli E, Vosnakidis T, et al. Mean platelet volume in patients with type 2 diabetes mellitus. Platelets 2004; 15(8): 475-8.
  10. Kodiatte TA, Manikyam UK, Rao SB, Jagadish TM, Reddy M, Lingaiah HK, et al. Mean platelet volume in type 2 diabetes mellitus. J Lab Physicians 2012; 4(1): 5-9.
  11. Uemura S, Matsushita H, Li W, Glassford AJ, Asagami T, Lee K-H, et al. Diabetes mellitus enhances vascular matrix metalloproteinase activity: role of oxidative stress. Circ Res 2001; 88(12): 1291-8.
  12. Mohammadi MT, Ramezani Binabaj M, Mirjalili MH, Gaedniaye Jahromi M, Jafari M, Salem F. Effect of atorvastatin on pancreatic oxidative stress in streptozotocin-induced diabetic rat. Iranian Journal of Endocrinology and Metabolism 2013; 15(2): 197-204.
  13. Kaneto H, Kawamori D, Matsuoka TA, Kajimoto Y, Yamasaki Y. Oxidative stress and pancreatic β-cell dysfunction. Am J Ther 2005; 12(6): 529-33.
  14. Pazdro R, Burgess JR. The role of vitamin e and oxidative stress in diabetes complications. Mech Ageing Dev 2010; 131(4): 276-86.
  15. Coruh N, Celep AS, Özgökçe F. Antioxidant properties of prangos ferulacea (L.) lindl., chaerophyllum macropodum Boiss. And Heracleum persicum desf. From apiaceae family used as food in eastern anatolia and their inhibitory effects on glutathione-S-transferase. Food Chem 2007; 100(3): 1237-42.
  16. Sadigh-Eteghad S, khayat-Nuri H, Abadi N, Ghavami S, Golabi M, Shanebandi D. Synergetic effects of oral administration of levamisole and echinacea purpurea on immune response in wistar rat. Res vet sci 2011; 91(1): 82-5.
  17. Hamidi S, Vaez H, Asgharian P. Rosa canina as an adjunctive treatment of asthma: A hypothesis. Adv Biosci Clin Med 2015; 3(1): 48-52.
  18. El-Masry AA: Effect of some medicinal plant oils on some physiological parameters in streptozotocin induced diabetic rat. Wudpecker J Medicin Plants 2011; 2: 22-35.
  19. Dutta M, Biswas UK, Chakraborty R, Banerjee P, Kumar A, Raychaudhuri U. Enhanced antioxidant enzymea ctivity in tissues and reduced total oxidative stress in plasma by the effect of swietenia macrophylla king seeds in type ii diabetes rats. Inter J Herb Med 2014; 1(6): 31-6.
  20. Colomeu TC, Figueiredo D, Cazarin CB, Schumacher NS, Marostica MR, MelettiLM, et al. Antioxidant and anti-diabetic potential of passiflora alata curtis aqueous leaves extract in type 1 diabetes mellitus (nod-mice). Int Immunopharmacol 2014; 18(1): 106-15.
  21. Demir F, Özcan M. Chemical and technological properties of rose (rosa canina L.) fruits grown wild in turkey. J Food Eng 2001; 47(4): 333-36.
  22. Kilicgun H, Altiner D. Correlation between antioxidant effect mechanisms and polyphenol content of rosa canina. Pharmacogn Mag 2010; 6(23): 238-41.
  23. Chrubasik C, Roufogalis BD, Müller‐Ladner U, Chrubasik S. A systematic review on the rosa canina effect and efficacy profiles. Phytother Res 2008; 22(6): 725-33.
  24. Tayefi‐Nasrabadi H, Sadigh‐Eteghad S, Aghdam Z. The effects of the hydroalcohol extract of rosa canina L. Fruit on experimentally nephrolithiasic wistar rats. Phytother Res 2012; 26(1): 78-85.
  25. Kirkeskov B, Christensen R, Bügel S, Bliddal H, Danneskiold-Samsøe B, Christensen LP, et al. The effects of rose hip (rosa canina) on plasma antioxidative activity and c-reactive protein in patients with rheumatoid arthritis and normal controls: A prospective cohort study. Phytomedicine 2011; 18(11): 953-8.
  26. Sadigh-Eteghad S, Tayefi-Nasrabadi H, Aghdam Z, Zarredar H, Shanehbandi D, Khayyat L, et al. Rosa canina L. Fruit hydro-alcoholic extract effects on some immunological and biochemical parameters in rats. BioImpacts 2011; 1(4): 219-24.
  27. Orhan N, Aslan M, Hosbas S, Deliorman OD. Antidiabetic effect and antioxidant potential of rosa canina fruits. Pharmacogn Mag 2009; 5(20): 309-15.
  28. Deeds MC, Anderson JM, Armstrong AS, Gastineau DA, Hiddinga HJ, Jahangir A, et al. Single dose streptozotocin-induced diabetes: Considerations for study design in islet transplantation models. Lab Anim 2011; 45(3): 131-40.
  29. Gireesh G, Thomas SK, Joseph B, Paulose C. Antihyperglycemic and insulin secretory activity of costus pictus leaf extract in streptozotocin induced diabetic rats and in in vitro pancreatic islet culture. J Ethnopharmacol 2009; 123(3): 470-4.
  30. Malek Mahdavi A, Mahdavi R, Kolahi S, Zemestani M, Vatankhah AM. L-carnitine supplementation improved clinical status without changing oxidative stress and lipid profile in women with knee osteoarthritis. Nutr Res 2015; 35(8): 707-15.
  31. Saliu J, Elekofehinti O, Komolafe K, Oboh G. Effects of some green leafy vegetables on the haematological parameters of diabetic rats. J Nat Prod Plant Resour 2012; 2(4): 482-5.
  32. Edet AE, Patrick EE, Olorunfemi A. Hematological parameters of alloxan-induced diabetic rats treated with ethanol extracts and fractions of nauclea lafiloia leaf. Eur Sci J 2013; 9(27): 203-210.
  33. Aladodo RA, Muhammad NO, Balogun EA. Effects of aqueous root extract of jatropha curcas on hyperglycaemic and haematological indices in alloxan-induced diabetic rats. Fountain J Nat App Sci 2013; 2(1): 52-8.
  34. Longe A, Momoh J, Adepoju P. Effects of cinnamon aqueous extract on blood glucose levell, liver biomarker enzymes, hematological and lipid profile parameters in aloxan-induced diabetic male albino rats. Eur Sci J 2015; 1: 418-26.
  35. Kanter M, Coskun O, Korkmaz A, Oter S. Effects of nigella sativa on oxidative stress and β‐cell damage in streptozotocin‐induced diabetic rats. Anat Rec A Discov Mol Cell Evol Biol 2004; 279(1): 685-91.
  36. Ugochukwu N, Babady N, Cobourne M, Gasset S. The effect of gongronema latifolium extracts on serum lipid profile and oxidative stress in hepatocytes of diabetic rats. J Biosci 2003; 28(1): 1-5.
  37. Fallahi F, Roghani M, Bagheri A. Time-dependent hypoglycemic and hypolipidemic effect of allium ascalonicum L. Feeding in diabetic rats. J Babol Univ Med Sci 2010; 12(1): 16-23.
  38. Khalili M, Vaez Mahdavi M, Kiasalari Z, Ansari F. The effect of long-term consumption of commiphora mukul feeding on the serum levels of glucose and lipids of diabetic rats. Iranian Journal of Endocrinology and Metabolism 2009; 11(1): 71-7.
  39. Morshedi A, Dashti M, Rafati A, Salami A, Mosaddegh M. The chronic effect of kombucha tea consumption on weight loss in diabetic rats. Journal of Medicinal Plants 2006; 5: 42-7.