Nanoparticles of copper and copper oxides: Synthesis and Determination of antibacterial activity

Document Type: Short Communication


1 Assistant Professor, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

3 Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran



In the present study, nanoparticles of copper and copper oxides were synthesized and their antibacterial activity was evaluated and compared with silver nanoparticles. The nanoparticles were synthesized using facile chemical reactions, and then characterized using field emission scanning microscopy. The nanoparticles were stable for at least two weeks. The antibacterial activity of the nanoparticles against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Enterococcusfaecalis and Escherichia coli was investigated based on inhibition zone in disk diffusion assay. The minimum inhibitory concentration and minimum bactericidal concentration of the nanoparticles were also reported. Antibacterial activity of the nanoparticles showed better inhibitory activity against gram positive bacteria.


  1. Nalwa HS. Encyclopedia of Nanoscience and Nanotechnology. vols 1-10. USA, California: American Scientific Publishers; 2004.
  2. Rahi A, Karimian K, Heli H. Nanostructured materials in electro analysis of pharmaceuticals. Anal Biochem 2016; 497, PP 39-47.
  3. Rizzello L, Pompa PP. Nano silver-based antibacterial drugs and devices: mechanisms, methodological drawbacks, and guidelines. Chem Soc Rev 2014; 43(5): 1501-18.
  4. Pelgrift RY, Friedman AJ. Nanotechnology as a therapeutic tool to combat microbial resistance. Adv Drug Deliv Rev 2013; 65(13-14): 1803-15.
  5. Chwalibog A, Sawosz E, Hotowy A, Szeliga J, Mitura S, Mitura K, Grodzik M, Orlowski P, Sokolowska A. Visualization of interaction between inorganic nanoparticles and bacteria or fungi. Int J Nanomedicine 2010; 5: 1085-94.
  6. Hajipour MJ, Fromm KM, Ashkarran AA, Jimenez de Aberasturi D, de Larramendi IR, Rojo T, Serpooshan V, Parak WJ, Mahmoudi M. Antibacterial properties of nanoparticles. Trends Biotechnol 2012; 30(10): 499-511.
  7. Effenberger F.B, Sulca M.A, Machini M.T, Couto R.A, Kiyohara P.K, Machado G, Rossi L.M. Copper nanoparticles synthesized by thermal decomposition in liquid phase: the influence of capping ligands on the synthesis and bactericidal activity. Journal of Nanoparticle Research 2014; 16: 2588.
  8. Jeziorska R, Zielecka M, Gutarowska B, Zakowska Z. High-density polyethylene composites filled with Nano silica containing immobilized Nano silver or Nano copper: thermal, mechanical, and bactericidal properties and morphology and interphase characterization. International Journal of Polymer Science 2014; pp. 1-13.
  9. Zain NM, Stapley AG, Shama G. Green synthesis of silver and copper nanoparticles using ascorbic acid and chitosan for antimicrobial applications. Carbohydr Polym 2014; 112: 195-202.
  10. Parks G.A. the isoelectric points of solid oxides, solid hydroxides, and aqueous hydroxo complex systems. Chemical Reviews 1965; 65(2): 177-198.