The Effect of Pine Cones Aqueous Extract on Renal Function in Male Rats

Document Type: Original Article


1 Department of Biochemistry, Fars Sciences and Researches Branch, Islamic Azad University, Shiraz, Iran

2 Associate Professor, Department of Biochemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran

3 Associate Professor, Department of Clinical Sciences, School of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran


Background:Antioxidant effects of cypress cones extract have been previously demonstrated. In this study, the protective effect of cypress cones extract was investigated. Gentamicin, an aminoglycoside antibiotic administrated for the treatment of gram-negative bacteria infections, was used for nephrotoxicity induction.
Methods: In this study, 60 wistar male rats were randomly divided into the six groups (n=10); control group (C) received nothing, sham group (S) received distilled water, group D received gentamicin for induction of nephrotoxicity, group GE received gentamicin as well as Cypress cones aqueous extract intra- peritoneally for 16 days, group DE received the extract after being sick and group E received just the extract. Sampling was done after 16 and 32 days of study. Sodium and potassium concentrations were measured using flame photometery method. Other parameters were assayed by colorimetric method.
Results: Statistically significant difference was detected between the control group and group D in regard to the mean level of Bun, creatinine, sodium, potassium, calcium and chloride (P<0.05), while no statistically significant difference was observed in the mean level of these parameters in DE and GE groups (the groups receiving Gentamicin and pine cones aqueous extract) in comparison with control group in days 16 and 32 (P>0.05). In this study, cypress cones aqueous extract could change the increased levels of Bun and serum creatinine and decreased levels of sodium, potassium, calcium and chloride resulted from kidney injury into their natural levels.
Conclusion: The present study revealed that cypress cones extract can improve Gentamicin-induced renal failure in rats. As previous studies have proved the existence of antioxidants in cypress cones, the observed health-promoting effects of aqueous extract can be attributed to these properties.


  1. Ghafari S, Esmaeili S, Naghibi F, Mosaddegh M. Plants used to treat “taberebá” (malaria like fever) in Iranian Traditional Medicine. International Journal of Traditional and Herbal Medicine 2013; 1(6):168-176.
  2. Gozelulusal B, Tufan H, Enginulusal A, Haberal c, Seyhan T, et al. Pretreatment with cypress cones water extract enhaces survival of is chemically challenged skin flaps a preliminary study. Turkish Journal of Plastic Surgery 2009; 17(1):25-9.
  3. Boukhnis M, Regane G, Yangui T, Sayadi S, Bouaziz M. Chemical composition and biological potential of essential oil from Tunisian Cupressussempervirens L. Journal of Arid Land Studies 2012;22(1):329-32.
  4. Koriem KM. Lead toxicity and the protective role of Cupressussempervirens seeds growing in Egypt. Rev LatinoamerQuím2009; 37(3):230-42.
  5. Zhang J, Rahman AA, Jains S, Jacob MR, Khan SI, Tekwani BL, et al. Antimicrobial and antiparasiticabietanediterpenoids from Cupressussempervirens. Research and reports in medicinal chemistry 2012; 2:1-6.
  6. Sanaa AA, Maha ZR, Nabawia AI, Mohga SA, Hayat MS, Magda MM. protective role of Juniperusphoenicea and Cupressussempervirens against CCl4. World J GastrointestPharmacolTher2010; 1(6):123-31.
  7. Karkabounas S, NikiforosKiortsis D, Zelovitis J, Skafida p, , Dementzos C Malamas M, Elisaf M, Evangelou A. Effects of Cupressussempervrenes cone extract on lipid parameters in Wistar rats . In Vivo 2003; 17(1): 101-104.
  8. Brahma SR, Suresh B, Elango K, Ramanathan M, Satishkumar MN. Wound healing properties of cupressussempervirens Linn. An International Journal of Advances Pharmacology Sciences 2012; 1(2): 231- 3.
  9. Maldonado PD,Barrera D, Medina- campos ON, Hernandez –Pando R, Ibarra-Rubio ME, Pedraza–cheverri J. Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats. Life Sci 2003; 73(20):2543-56.
  10. Dehgani F, Namavar MR, Noorafshan A, Karbalay-dous S, Esmael pour T. Evaluation of the kidney extract on gentamicin induced–nephrotoxcity in rat. Kidney Research Journal2011; 1(1):24-32.
  11. Emami SA, Asili J, Rahimizadeh M, Fazely-Bazzaz BS, Hassazadeh-Khayyat M. Chemical and antimicrobial studies of Cupressus semper virenes and C. horizentalis Mill. Essential oils. Iranian Journal of Pharmaceutical Sciences 2006; 2(2): 103-8.
  12. Amiri I, Hamrouni L, Hanane M, Gargouri S, Jamoussi B. Chemical composition bio-herbicidal and antifungal activities of essential oils isolated from Tunisian common cypress (Cupressussempervirnes L). Journal of Medical Plants Research 2013; 7(16): 1070-80.
  13. TumenIB, SezerSenolF, OrhanIE. Evaluation of possible in vitro nevrobiological effects of two varieties of cupressussempervirems (Mediterranean cypress) through their antioxidant and enzyme inhibition actions. Turkish Journal of Biochemistry 2012; 37(1):5-13.
  14. Nusaibah A. The histological changes on liver and spleen of Mice treated with Alcoholic and Aquatic Extract of Cupressussempervirens. Al-Anbar Medical Journal 2012; 10(2): 51-7.
  15. Panahi M, Namjoyan F, Shakerin Z. Evaluation of antioxidant effects of nigella sativa extract on the ultera structure of neural tube defects in diabetic rats offspring. Jundishapur Journal of Natural Pharmaceutical Products 2011; 6(1):16-23.
  16. Ahmadi M, Hajihashemi S, Chehrei A, Hosseini N. Therapeutic effects of Urticadioicamethanolic extract on gentamicin induced nephrotoxicity in rats. Koomesh 2014; 15(2):220-31. [In Persian].
  17. Bonsnese RW,Taussky HH.On the colorimetric of creatinine by the reaction. JBiolChem1945; 158:581-91.
  18. Tietze NW. Fundamental of Clinical Chemistry.3thed.Philadelphia: Saunders; 1987.
  19. Lubran MM. The measurement of total serum proteins by the biuret method. Annals of Clinical and Laboratory Sciences 1978; 8(2):106-10.
  20. Stockhom SL, Scott MA. Fundamentals of Veterinary Clinical Pathology.2thed.Ames, Iowa, USA: Wiley-Blackwell; 2008.
  21. Watkins BA, Reinwald S, Li Y, Seifert MF. Protective actions of soy isoflavones and n-3 PUFAs on bone mass in ovariectomized rats. J NutrBiochem 2005; 16(8):479-88.
  22. Kadir FA, Kassim NM, Abdulla MA, Yehye WA. Effect of oral administration of ethanolicextract of Vitexnegundo on thioacetamide-induced nephrotoxicity in rats. BMC Complement Altern Med 2013;13:294.
  23. Denamur S, Tyteca D, Marchand-Brynaert J, Van Bambeke F, Tulkens PM, Courtoy PJ, et al. Role of oxidative stress in lysosomal membrane permeabilization and apoptosis induced by gentamicin, an aminoglycoside antibiotic. Free RadicBiol Med 2011; 51(9):1656-65.
  24. Farombi EO, Ekor M. Curcumin attenuates gentamicin-induced renal oxidative damage in rats. Food ChemToxicol 2006; 44(9):1443-8.
  25. Amini FG, Rafieian-Kopaei M, Nematbakhsh M, Baradaran A, Nasri H. Ameliorative effects of metformin on renal histologic and biochemical alterations of gentamicin-induced renal toxicity in Wistar rats. J Res Med Sci 2012; 17(7): 621–5.
  26. Karahan I, Ateşşahin A, Yilmaz S, Ceribaşi AO, Sakin F. Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. Toxicology 2005; 215(3):198-204.
  27. Ibrahim NA, El-Seedi HR, Mohammed MM. Phytochemical investigation and hepatoprotective activity of Cupressussempervirens L. leaves growing in Egypt. Nat Prod Res 2007; 21(10):857-66.
  28. Weigert P, Scheck K, Lemmer B, Noreisch W. Laboratory diagnostic studies of haflinger horses and mules (pack-animals of the Federal German Army). 2. Enzyme activity in serum. TierarztlPrax1980; 8(3):387-93.
  29. Stuart F. Human Physiology. Translated by Dilmaghani J, Gharibzadeh S. 1th ed. Tehran: Iran University of Medical Sciences; 2004. p. 426-75.
  30. Ciric M, Radenkovic M, Nesic M, Cekic S, Puskas N, Stoilakovic N, et al. The effects of Enalappil on experimental gentamicin nephrotoxicity. ActaMedicaMedianae2014; 53(2):16-21.
  31. Parsons PP, Garland HO, Harpur ES. Localization of the nephron site of gentamicin-induced hypercalciuria in the rat: a micropuncture study.Br J Pharmacol 2000; 130(2):441-9.
  32. Makhloogh A. Aminoglycosides and Nephrotoxisity. Mazandaran Medicine University and Health Services. Surrey Journal of Food and Drug Department and Faculty of Pharmacy 2012; 16:16-20.
  33. Garry F, Chew DJ, Hoffsis GF. Enzymuria as an index of renal damage in sheep with induced aminoglycoside nephrotoxicosis. Am J Vet Res1990; 51(3):428-32.
  34. Milos M, Mastelic J, Radonic A. Free and Glycosidically Bound Volatile Compounds from Cypress Cones (CupressusSempervirens L.). CroaticaChemica Acta1998; 71(1): 139-45. 
  35. Gowrisi M, Sarita K, VrushabendraSwamy BM, KotagiriS, VishwanathKM. Anti-oxidant and nephroprotective activities of cassia occidentalis leaf extract against gentamicin induced nephrotoxicity in rats. ResearchJournal of Pharmaceutical, Biological and Chemical Sciences 2012; 3(3):684-94.
  36. NamjooA, Sadri S M, Rafeian M, Ashrafi K, ShahinFard N, Ansari Samani R, et al. Comparing the effects of nigella sativa extract and gentamicin in treatment of urinary tract infection caused by Ecoli. J MazandaranUniv Med Sci 2013; 22(96):22-9.[In Persian].
  37. Ghaznavi R, Kadkhodaee M, Khastar H, Zahmatkesh M. Renal oxidative stress status and histology in gentamicin nephrotoxicity: The effects of antioxidant vitamins. Tehran Univ Med J 2006; 64(5):15-22.[In Persian].
  38. Kadir FA, Kassim NM, Abdulla MA, Yehye WA. Effect of oral administration of ethanolicextract of Vitexnegundo on thioacetamide-induced nephrotoxicity in rats. BMC Complement Altern Med 2013; 13:294.
  39. Johari H, SharifiE, Delirnasab F, Hemayatkhah V, Kargar H. The effect of hydro-alcoholic extracts of ginger on lead detoxification of kidney in the immature Wistar rats. Journal of Rafsanjan University of Medical Sciences 2012; 12(6):417-24. [In Persian].
  40. GhasemiPirbalouti A, Shahvali A, Saghaee F, Azizi S, Hamedi B, Shahgholian L. Effect of Cichoriumintybus L. extracts and KelussiaoderatassimaMozaff. Essential oil on toxic of organophosphouros insecticides in rat. Journal of Herbal Drugs (An International Journal on Medicinal Herbs) 2010; 1(2):30-5.[In Persian].
  41. Nouri M, Khaki A, Fathi Azad F, Rezazadeh H, Doulatkhah H, Vatankhah AM. Antioxidant effect of carrot seed and it's protective role in gentamicin receiving rats. Journal of Tabriz University of Medical Sciences 2009; 31(1):79-84. [In Persian].