Polystyrene-Hydroxyapatite Nanocomposite as a New Adsorbent of Aflatoxin M1

Document Type: Original Article

Authors

1 M.Sc. Student, Department of Food Science and Technology, School of Agriculture, Yazd Science and Research Branch, Islamic Azad University, Yazd, Iran

2 Assistant Professor, Department of Food Science and Technology, School of Agriculture, Yazd Science and Research Branch, Islamic Azad University, Yazd, Iran

3 Assistant Professor, Department of Laboratory Sciences, School of Allied Medical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Abstract

Background & Aims: Aflatoxin M1 is one of the most important toxins present in milk and the product of aflatoxin B1 metabolism in liver microsomes. The aim of this project was to study the aflatoxin M1 adsorption ability of polystyrene-hydroxyapatite nanocomposite.
Methods: First, the probability of aflatoxin M1 adsorption by the nanocomposite was evaluated using the HyperChem simulation software. Then, polystyrene-hydroxyapatite nanocomposite was synthesized and its properties were determined through chemical deposition. Subsequently, serial concentrations of the nanocomposite were prepared and separately incubated with aflatoxin M1 solution at different temperatures and pH. Finally, the adsorption quantity was measured through high performance liquid chromatography (HPLC).
Results: The computer simulation showed that aflatoxin M1 was adsorbed by the nanocomposite. In addition, scanning electron microscope (SEM) images showed that polystyrene and hydroxyapatite nanoparticles were formed and formed a nanocomposite. Image analysis showed that the nanoparticle size distribution was between 50-500 nm. This study revealed that although the adsorption quantity was not affected by temperature, it was affected by pH in all concentrations. The adsorption quantity in a pH of 5 was higher than that in pH of 7 and 9.
Conclusion: In the present study, it was shown for the first time that polystyrene-hydroxyapatite nanocomposite can adsorb aflatoxin M1. This study also demonstrated that although the adsorption was not affected by temperature, pH remarkably affected it.

Keywords


  1. Brase S, Glaser F, Kramer CS, Lindner S, Linsenmeier AM, Masters KS, et al. Progress in the chemistry of organic natural products. The chemistry of mycotoxins. Prog Chem Org Nat Prod 2013; 97: v-300.
  2. Matsuda Y, Wakai T, Kubota M, Osawa M, Sanpei A, Fujimaki S. Mycotoxins are conventional and novel risk biomarkers for hepatocellular carcinoma. World J Gastroenterol 2013; 19(17): 2587-90.
  3. Mohd-Redzwan S, Jamaluddin R, Abd-Mutalib MS, Ahmad Z. A mini review on aflatoxin exposure in Malaysia: past, present and future. Front Microbiol 2013; 4: 334.
  4. Ibeh IN, Saxena DK, Uraih N. Toxicity of aflatoxin: effects on spermatozoa, oocytes, and in vitro fertilization. J Environ Pathol Toxicol Oncol 2000; 19(4): 357-61.
  5. Moudgil V, Redhu D, Dhanda S. A review of molecular mechanisms in the development of hepatocellular carcinoma by aflatoxin and hepatitis B and C viruses. Journal of Environmental Pathology Toxicology and Oncology 2013; 32(2): 165-75.
  6. Milita NM, Mihaescu G, Chifiriuc C. [Aflatoxins--health risk factors]. Bacteriol Virusol Parazitol Epidemiol 2010; 55(1): 19-24.
  7. Caloni F, Cortinovis C, Pizzo F, de Angelis I. Transport of aflatoxin M1 in human intestinal Caco-2/TC7 cells. Front Pharmacol 2012; 3: 111.
  8. Wild C, Fortuin M, Donato F, Whittle HC, Hall A, Wolf CR, et al. Aflatoxin, liver enzymes, and hepatitis B virus infection in Gambian children. Cancer Epidemiol Biomarkers Prev 1993; 2: 555.
  9. Choudhary P, Sharma R, Borkhartria V. Effect of chilling and heating on aflatoxin M1 content of contaminated Indian cow's milk. Egyptian Journal of Dairy Science 1998; 26(2): 223-9.
  10. Kutz RE, Sampson JD, Pompeu LB, Ledoux DR, Spain JN, Vazquez-Anon M, et al. Efficacy of Solis, NovasilPlus, and MTB-100 to reduce aflatoxin M1 levels in milk of early to mid-lactation dairy cows fed aflatoxin B1. J Dairy Sci 2009; 92(8): 3959-63.
  11. Phillips TD, Kubena LF, Harvey RB, Taylor DR, Heidelbaugh ND. Hydrated sodium calcium aluminosilicate: a high affinity sorbent for aflatoxin. Poult Sci 1988; 67(2): 243-7.
  12. Phillips TD, Clement BA, Kubena LF, Harvey RB. Detection and detoxification of aflatoxins: prevention of aflatoxicosis and aflatoxin residues with hydrated sodium calcium aluminosilicate. Vet Hum Toxicol 1990; 32(Suppl): 15-9.
  13. Phillips TD, Sarr AB, Grant PG. Selective chemisorption and detoxification of aflatoxins by phyllosilicate clay. Nat Toxins 1995; 3(4): 204-13.
  14. Galvano F, Pietri A, Bertuzzi T, Fusconi G, Galvano M, Piva A, et al. Reduction of carryover of aflatoxin from cow feed to milk by addition of activated carbons. Journal of Food Protection 1996; 5: 448-561.
  15. Lickly TD, Breder CV, Rainey ML. A model for estimating the daily dietary intake of a substance from food-contact articles: styrene from polystyrene food-contact polymers. Regul Toxicol Pharmacol 1995; 21(3): 406-17.
  16. Hu Q, Li B, Wang M, Shen J. Preparation and characterization of biodegradable chitosan/hydroxyapatite nanocomposite rods via in situ hybridization: a potential material as internal fixation of bone fracture. Biomaterials 2004; 25(5): 779-85.
  17. Jebali A, Yasini Ardakani A, Sedighi N, Hekmatimoghaddam SH. Erratum to: Nanocellulose conjugated with retinoic acid: its capability to adsorb aflatoxin B1. Cellulose 2015; 22(3): 2139.
  18. Saffarzadeh N, Kalantar SM, Jebali A, Hekmatimoghaddam SH, Sheikhha MH, Farashahi E. The cellular uptake of antisense oligonucleotid of E6 mRNA into cervical cancer cells by DOPE-modified hydroxyapatite nanoparticles. Nanomedicine Journal 2014; 1(5): 292-7.
  19. Zhang SW, Zhou SX, Weng YM, Wu LM. Synthesis of SiO2/polystyrene nanocomposite particles via miniemulsion polymerization. Langmuir 2005; 21(6): 2124-8.
  20. Kim EK, Shon DH, Ryu D, Park JW, Hwang HJ, Kim YB. Occurrence of aflatoxin M1 in Korean dairy products determined by ELISA and HPLC. Food Addit Contam 2000; 17(1): 59-64.
  21. Thom DC, Davies JE, Santerre JP, Friedman S. The hemolytic and cytotoxic properties of a zeolite-containing root filling material in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003; 95(1): 101-8.