Mumijo Protection gainst Acetaminophen-Induced Acute Hepatic Injury: Role of Oxidative Stress

Document Type: Original Article

Authors

1 MSc Student in Physiology, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

2 Associate Professor of Physiology, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences , Kerman University of Medical Sciences, Kerman, Iran

3 Professor of Physiology, Endocrinology and Metabolism Research Center, Kerman University of Medical Sciences, Kerman, Iran

4 Professor of Biochemistry, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

5 Medical Student, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

6 MSc Student in Physiology, Gastroenterology and Hhepatology Research Center, Kerman University of Medical Sciences, Kerman, Iran

Abstract

Background: A majority of people widely use acetaminophen as a sedative. Overusing the drug for prolonged periods of time can lead to acute liver damage. Mumijo, as a strong antioxidant and anti-inflammatory drug, could possibly reduce some of the acetaminophen-induced side effects on the liver. Thus, the aim of this study is to evaluate the effect of Mumijo on the liver damage caused by the use of acetaminophen.
Methods: 40 male Wistar rats were randomly divided into five groups: sham, acetaminophen, low and high doses of mumijo, and vehicle. All groups except the sham group received a single dose of 500 mg/kg acetaminophen via ip injection. Then the groups that were under treatment received 150 mg/kg (low dose) and 250 mg/kg (high dose) of mumijo, and the vehicle group received distilled water as vehicle. After 24 hours, blood samples were taken for biochemical tests, and a part of the liver was extracted for histopathological examination.
Results: acetaminophen increases the activities of functional liver enzymes including alanine amino transferase (ALT), aspartate aminotransferase (AST), and gamma glutamine transferase (GGT). In groups under treatment, values of the mentioned enzymes were significantly reduced in comparison with the acetaminophen and vehicle groups (P <0.05), and on the other hand, malondialdehyde (MDA), nitric oxide (NO), and protein carbonyl (PC) increase caused by acetaminophen were reduced by mumijo. Furthermore, the amount of glutathione (GPX) was increased by mumijo (P <0.05). From a histopathological point of view, necrosis and liver damage caused by acetaminophen was decreased by mumijo.
Conclusion:The findings showed that mumijo is salient in preventing liver damage caused by consumption of high doses of acetaminophen probably through reducing oxidant activities and also through increasing anti-inflammatory and antioxidant activities.
 

Keywords


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