Myofibroblasts in calcifying odontogenic cyst and dentigerous cyst

Document Type: Original Article


1 Student Research Committee, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran

2 Assistant Professor, Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

3 Department of Pathology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

4 Assistant Professor, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

5 Health Research Institute, Babol University of Medical Sciences, Babol, Iran


Introduction:Calcifying odontogenic cyst (COC) is an odontogenic cyst that sometimes shows aggressive behavior, while dentigerous cyst (DC) always has a benign nonaggressive course. There are evidences that myofibroblasts are involved in invasion and their role in biologic behavior of odontogenic cysts has been less understood. So, the aim of the present study was to compare the role of myofibroblasts in COC and DC.
Methods:In this cross-sectional study, paraffin-embedded tissue blocks of 20 COCs and 20 DCs were studied. Four-micron sections were prepared from tissue blocks and stained with α-SMA antibody using immunohistochemistry. Percentages of myofibroblasts were semi quantitatively classified into negative (<5% of cells were positive), weak (5-50% cells were positive) and strong (>50% of cells were positive). Percentage of myofibroblasts was compared between COC and DC groups using t-test and Chi-Square statistical tests.
Results:Means of myofibroblasts percentages in COC and DC groups were respectively 35±33 and 32±30 that did not show significant difference between COC and DC groups (P=0.76). There was not any significant difference between COC and DC groups with respect to semi quantitative classification of percentage of myofibroblasts (P=0.62).
Conclusion:Myofibroblasts probably do not play a significant role in different biologic behaviors of calcifying odontogenic and dentigerous cysts.


  1. Rajabi-Moghaddam M, Abbaszadeh-Bidokhty H, Bijani A. Comparison of mast cells count in odontogenic cysts using histochemical staining. Iran J Pathol. 2015;10(2):105-110.
  2. Neville BW, Daam DD, Allen CM, Chi AC. Oral and maxillofacial pathology.4thed., Missouri, Saunders Co., 2016; PP 647-8.
  3. Thinakaran M, Sivakumar P, Ramalingam S, Jeddy N, Balaguhan S. Calcifying ghost cell odontogenic cyst: A review on terminologies and classifications. J oral maxillofac pathol 2012; 16 (3):450-3.
  4. Eshghyar N, Jalayer-Nadery N, Ashery R. Calcifying odontogenic cyst: an analysis of thirty-six cases. Acta Medica Iranica 2006 ;44 (1): 59-62.
  5. Dodani A, Siadati S, Hajian-Tilaki K, Abbaszadeh H. Comparative evaluation of the frequency of myofibroblasts between oral and cutaneous squamous cell carcinomas. Caspian J Dent Res 2016; 5 (2):24-9.
  6. Nadalin MR, Fregnani ER, Silva-Sousa YT, da Cruz Perez DE. Presence of myofibroblasts and matrix metalloproteinase 2 in radicular cysts, dentigerous cysts, and keratocystic odontogenic tumors: a comparative immunohistochemical study. J endod 2012; 38 (10):1363-7.
  7. Mashhadiabbas F, Atarbashi Moghadam S, Moshref M, Elahi M. Immunohistochemical detection and ultrastructure of myofibroblasts in the stroma of odontogenic cysts and ameloblastoma. Iran Red Crescent Med J 2010; 12(4): 453-7.
  8. Vered M, Shohat I, Buchner A, Dayan D. Myofibroblasts in stroma of odontogenic cysts and tumors can contribute to variations in the biological behavior of lesions. Oral Oncol 2005; 41 (10):1028-33.
  9. Syamala D, Suresh R, Janardhanan M, Savithri V, Anand PP, Jose A. Immunohistochemical evaluation of myofibroblasts in odontogenic cysts and tumors: A comparative study. J oral maxillofac pathol 2016; 20(2): 208-13.
  10. Kouhsoltani M, Halimi M, Jabbari G. Immunohistochemical evaluation of myofibroblast density in odontogenic cysts and tumors. J Dent Res Dent Clin Dent Prospects 2016;10(1):37-42.