Reduction of Saliva and Serum 25-Hydroxycholecalciferol in Multiple Sclerosis

Document Type: Original Article


1 Student Research Committee, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

2 Student Research Committee, School of Dentistry, Aja University of Medical Sciences; Tehran, Iran

3 Professor Department of Physiology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran


Methods:The prevalence of multiple sclerosis (MS) is high in patients suffering from 25-hydroxyvitamin D3 [25(OH)D] deficiency. The aim of this study was to investigate the possible association of saliva and serum 25(OH)D concentrations with MS in women.
Methods: Serum and saliva 25(OH)D levels of 30 MS women and 30 matched healthy controls in this case-control study were measured by ELISA. Data were analyzed by unpaired two-tailed student’s t-test, Pearson correlation test and Receiver operating characteristic (ROC).
Results: The mean levels of 25(OH)D in serum and in both stimulated and unstimulated saliva were significantly lower in patients with MS. Serum levels of 25(OH) significantly correlated with stimulated (r = 0.575; P=0.003) and unstimulated saliva (r = 0.548; P=0.004). The mean (±SD) EDSS was 3.6±1.9 in the MS group. EDSS was not significantly correlated with 25(OH)D in serum or in stimulated and unstimulated saliva. The cut-off points of 25(OH)D in serum and saliva were 18 ng/ml and 65 pg/ml, respectively.
Conclusions:25(OH)D level in saliva like in serum was low in MS women. Serum 25(OH)D levels correlated positively with saliva 25(OH)D in women suffering from MS.


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