Effect of Troxerutin on Oxidative Stress Induced by Sciatic Nerve Ischemia-reperfusion Injury in Rats

Document Type : Original Article


1 M.Sc. of Physiology, Department of Physiology, Iranshahr University of Medical Sciences, Iranshahr, Iran

2 Professor in Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

3 MSc. of Anatomical Scinces, Department of Anatomy, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran and Department of Anatomical Sciences, Afzalipour Medical School, Kerman University of Medical Sciences, Kerman, Iran

4 M.Sc. of Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

5 Ph.D. Student in Clinical Biochemistry, Department of Clinical Biochemistry, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6 Student of Medicine, Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran

7 Ph.D. Student in Clinical Biochemistry, Razi Herbal Medicines Researches Center, Lorestan University of Medical Sciences, Khorramabad, Iran


Background:Troxerutin has antioxidant and anti-inflammatory properties and in this study, its antioxidant effect on the reduction of oxidative stress induced by ischemia-reperfusion sciatic nerve injury was investigated.
Methods:In this study, 64 male rats were randomly divided into 8 groups as follows: 1- IR2: ischemia (3 hours) and reperfusion (2 days), 2- Trox+IR2: ischemia (3 hours) and reperfusion (2 days), 3- IR7: ischemia (3 hours) and reperfusion (7 days), 4- Trox+IR7: ischemia (3 hours) and reperfusion (7 days), 5- IR14: ischemia (3 hours) and reperfusion (14 days), 6- Trox+IR14: ischemia (3 hours) and reperfusion (14 days), 7- IR28: ischemia (3 hours) and reperfusion (28 days), 8- Trox+IR28: ischemia (3 hours) and reperfusion (28 days). The rats received 150 mg/kg troxerutin in one injection (single dose). After separation of serum, biochemical parameters of the serums such as NO, PON1, CAT, and GPX were measured.
Results:Troxerutin significantly increased the GPX and PON1 levels in groups that their reperfusion time was 2 and 14 days (p <0.05). There was no significant difference in the levels of NO and CAT between the groups received troxerutin and control groups (P>0.05).
Conclusion:Troxerutin relatively decreased the oxidative stress in the sciatic nerve ischemia-reperfusion injury by increasing the level of antioxidant enzymes.


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