Effect of Troxerutin on Oxidative Stress Induced by Sciatic Nerve Ischemia-reperfusion Injury in Rats

Document Type: Original Article

Authors

1 M.Sc. of Physiology, Department of Physiology, Iranshahr University of Medical Sciences, Iranshahr, Iran

2 Professor in Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

3 MSc. of Anatomical Scinces, Department of Anatomy, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran and Department of Anatomical Sciences, Afzalipour Medical School, Kerman University of Medical Sciences, Kerman, Iran

4 M.Sc. of Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

5 Ph.D. Student in Clinical Biochemistry, Department of Clinical Biochemistry, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6 Student of Medicine, Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran

7 Ph.D. Student in Clinical Biochemistry, Razi Herbal Medicines Researches Center, Lorestan University of Medical Sciences, Khorramabad, Iran

10.22062/jkmu.2020.91020

Abstract

Background:Troxerutin has antioxidant and anti-inflammatory properties and in this study, its antioxidant effect on the reduction of oxidative stress induced by ischemia-reperfusion sciatic nerve injury was investigated.
Methods:In this study, 64 male rats were randomly divided into 8 groups as follows: 1- IR2: ischemia (3 hours) and reperfusion (2 days), 2- Trox+IR2: ischemia (3 hours) and reperfusion (2 days), 3- IR7: ischemia (3 hours) and reperfusion (7 days), 4- Trox+IR7: ischemia (3 hours) and reperfusion (7 days), 5- IR14: ischemia (3 hours) and reperfusion (14 days), 6- Trox+IR14: ischemia (3 hours) and reperfusion (14 days), 7- IR28: ischemia (3 hours) and reperfusion (28 days), 8- Trox+IR28: ischemia (3 hours) and reperfusion (28 days). The rats received 150 mg/kg troxerutin in one injection (single dose). After separation of serum, biochemical parameters of the serums such as NO, PON1, CAT, and GPX were measured.
Results:Troxerutin significantly increased the GPX and PON1 levels in groups that their reperfusion time was 2 and 14 days (P<0.05). There was no significant difference in the levels of NO and CAT between the groups received troxerutin and control groups (P>0.05).
Conclusion:Troxerutin relatively decreased the oxidative stress in the sciatic nerve ischemia-reperfusion injury by increasing the level of antioxidant enzymes.

Keywords


  1. McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 1985; 312(3):159-63.
  2. Huang L, Chen N, Ge M, Zhu Y, Guan S, Wang JH. Ca2+ and acidosis synergistically lead to the dysfunction of cortical GABAergic neurons during ischemia. Biochemical and Biophysical Research Communications 2010; 394(3):709-14.
  3. Šimonová Z, Štěrbová K, Brožek G, Komárek V, Syková E. Postnatal hypobaric hypoxia in rats impairs water maze learning and the morphology of neurones and macroglia in cortex and hippocampus. Behavioral Brain Research 2003; 141(2):195-205.
  4. Jafari M. Dose-and time-dependent effects of sulfur mustard on antioxidant system in liver and brain of rat. Toxicology 2007; 231(1):30-9.
  5. Salehipour M, Monabbati A, Salahi H, Nikeghbalian S, Bahador A, Marvasti VE, et al. Protective effect of parenteral vitamin E on ischemia-reperfusion injury of rabbit kidney. Urology 2010; 75(4):858-61.
  6. Maurya DK, Salvi VP, Nair CK. Radioprotection of normal tissues in tumor-bearing mice by troxerutin. J Radiat Res 2004; 45(2):221-8.
  7. Vinothkumar R, Vinoth Kumar R, Karthikkumar V, Viswanathan P, Kabalimoorthy J, Nalini N. Oral supplementation with troxerutin (trihydroxyethylrutin), modulates lipid peroxidation and antioxidant status in 1, 2-dimethylhydrazine-induced rat colon carcinogenesis. Environ Toxicol Pharmacol 2014; 37(1):174-84.
  8. Kessler M, Ubeaud G, Walter T, Sturm F, Jung L. Free radical scavenging and skin penetration of troxerutin and vitamin derivatives. J Dermatolog Treat 2002; 13(3):133-41.
  9. Milcan A, Arslan E, Bagdatoglu OT, Bagdatoglu C, Polat G, Kanik A, et al. The effect of alprostadil on ischemia–reperfusion injury of peripheral nerve in rats. Pharmacological Research 2004; 49(1):67-72.
  10. Saray A, Apan A, Kisa U. Free radical-induced damage in experimental peripheral nerve injection injury. J Reconstr Microsurg 2003; 19(6):401-6.
  11. Iida H, Schmelzer JD, Schmeichel AM, Wang Y, Low PA. Peripheral nerve ischemia: reperfusion injury and fiber regeneration. Exp Neurol 2003; 184(2):997-1002.
  12. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra W. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973; 179(4073):588-90.
  13. Ahmadvand H, Yalameha B, Adibhesami G, Nasri M, Naderi N, Babaeenezhad E, et al. The protective role of gallic acid pretreatment on renal ischemia-reperfusion injury in rats. Rep Biochem Mol Biol 2019; 8(1):42-8.
  14. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105:121-6.
  15. Nasri M, Mahdavifard S, Babaeenezhad E, Adibhesami G, Nouryazdan N, Veiskarami S, et al. Ameliorative effects of histidine on oxidative stress, tumor necrosis factor alpha (TNF-α), and renal histological alterations in streptozotocin/nicotinamide-induced type 2 diabetic rats. Iran J Basic Med Sci 2020; 23(6):714-23.
  16. Blatter MC, James RW, Messmer S, Barja F, Pometta D. Identification of a distinct human high‐density lipoprotein subspecies defined by a lipoprotein‐associated protein, K‐45: Identity of K‐45 with paraoxonase. European Journal of Biochemistry 1993; 211(3):871-9.
  17. Griess P. Ber. Deutsch Chem. Ges 1879; 12: 426.
  18. Tokmak M, Sehitoglu MH, Yuksel Y, Guven M, Akman T, Aras AB, et al. The axon protective effects of syringic acid on ischemia/reperfusion injury in a rat sciatic nerve model. Turk Neurosurg 2017; 27(1):124-32.
  19. Bridges D, Thompson SW, Rice AS. Mechanisms of neuropathic pain. British Journal of Anaesthesia 2001; 87(1):12-26.
  20. Adil M, Kandhare AD, Visnagri A, Bodhankar SL. Naringin ameliorates sodium arsenite-induced renal and hepatic toxicity in rats: decisive role of KIM-1, Caspase-3, TGF-β, and TNF-α. Ren Fail 2015; 37(8):1396-407.
  21. Levy D, Zochodne DW. NO pain: potential roles of nitric oxide in neuropathic pain. Pain Pract 2004; 4(1):11-8.
  22. Kandhare AD, Mukherjee AA, Bodhankar SL. Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: Evidence from anti-inflammatory, antioxidant and anti-apoptotic studies. EXCLI J 2017; 16:546-65.
  23. Gholami M, Khanipour Khayat Z, Anbari K, Obidavi Z, Varzi A, Boroujeni MB, et al. Quercetin ameliorates peripheral nerve ischemia–reperfusion injury through the NF-kappa B pathway. Anat Sci Int 2017; 92(3):330-7.
  24. Zhang MT, Wang B, Jia YN, Liu N, Ma PS, Gong SS, et al. Neuroprotective effect of liquiritin against neuropathic pain induced by chronic constriction injury of the sciatic nerve in mice. Biomedicine & Pharmacotherapy 2017; 95:186-98.
  25. Guven M, Gölge UH, Aslan E, Sehitoglu MH, Aras AB, Akman T, et al. The effect of aloe vera on ischemia—Reperfusion injury of sciatic nerve in rats. Biomed Pharmacother 2016; 79:201-7.
  26. Yu Y, Zheng G. Troxerutin protects against diabetic cardiomyopathy through NF‑κB/AKT/IRS1 in a rat model of type 2 diabetes. Mol Med Rep 2017; 15(6):3473-8.
  27. Najafi M, Noroozi E, Javadi A, Badalzadeh R. Anti-arrhythmogenic and anti-inflammatory effects of troxerutin in ischemia/reperfusion injury of diabetic myocardium. Biomed Pharmacother 2018; 102:385-91.
  28. Badalzadeh R, Chodari L, Ghorbanzadeh V. Troxerutin, a bioflavonoid, improves oxidative stress in blood of streptozotocin-induced type-1 diabetic rats. Iranian Journal of Pharmaceutical Sciences 2017; 13(2):75-86.
  29. Elangovan P, Ramakrishnan R, Amudha K, Jalaludeen AM, Sagaran GK, Babu FR, et al. Beneficial Protective Effect of Troxerutin on Nickel-Induced Renal Dysfunction in Wistar Rats. J Environ Pathol Toxicol Oncol 2018; 37(1):1-14.
  30. Raja B, Saranya D, Prabhu R. Role of flavonoid troxerutin on blood pressure, oxidative stress and regulation of lipid metabolism. Front Biosci (Elite Ed) 2019; 11:121-9.
  31. Jalali M, Hassanipour M, Hajizadeh M, Khanamani Falahati Pour S, Khoshdel A, Roustai F, et al. Troxerutin chronic treatment protects against fructose-induced metabolic syndrome in male rats. International Journal of Medical Laboratory 2017; 4(3):201-10.
  32. Costa LG, Cole TB, Garrick JM, Marsillach J, Furlong CE. Metals and paraoxonases. Adv Neurobiol 2017; 18:85-111.
  33. Farajdokht F, Amani M, Mirzaei Bavil F, Alihemmati A, Mohaddes G, Babri S. Troxerutin protects hippocampal neurons against amyloid beta-induced oxidative stress and apoptosis. EXCLI J 2017; 16:1081-9.
  34. Thomas NS, George K, Arivalagan S, Mani V, Siddique AI, Namasivayam N. The in vivo antineoplastic and therapeutic efficacy of troxerutin on rat preneoplastic liver: biochemical, histological and cellular aspects. Eur J Nutr 2017; 56(7):2353-66.