The Effect of High-intensity Interval Training and L-carnitine on the Expression of Some Pro-inflammatory Genes in the Liver and Cardiac Tissues of Rats

Document Type : Original Article


1 Assistant Professor, Student Research Committee & Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

2 Associate Professor, Cardiovascular Research Center & Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

3 Assistant Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

4 Ph.D. Candidate, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran



Background: Inflammation is characterized by interactions between cytokines and inflammatory pathogens. Cytokines are glycoproteins involved in inflammation. Exercise training and physical activity are associated with a healthy lifestyle. In the current study, we aimed to determine the effects of high intensity interval training (HIIT) and L-Carnitine (LCAR) on the expression of genes involved in inflammation and inflammatory pathways in the liver and heart of rats.
Method:Thirty-two male Wistar rats were randomly divided into the four groups (n = 8): 1. Untreated control group, 2. LCAR group (received 200 mg/kg LCAR daily), 3. HIIT group (performed high intensity interval training), 4. Exercise training + LCAR.
Results:The results of our study showed that HIIT + LCAR significantly reduced the expression of IL-1β in the liver compared to the HIIT group (p = 0.038). The combination of HIIT and LCAR decreased IL-6 expression in the liver tissue compared to the control (p <0.001), LCAR (p <0.001), and HIIT (p = 0.002) groups. The HIIT + LCAR group decreased Cox 2 gene expression in the liver tissue compared to the untreated control group (p <0.001), and LCAR group (p = 0.007). The combination of HIIT and LCAR reduced IL-1β expression in the cardiac tissue compared to the untreated control (p <0.001), LCAR (p = 0.034), and HIIT (p = 0.041) groups. The combination of HIIT and LCAR increased IL-6 expression in the cardiac tissue compared to the other three groups (p <0.001).
Conclusion:According to the obtained results, HIIT combined with LCAR administration is very useful in reducing the expression of pro-inflammatory genes in the heart and liver tissues.


Petersen AM, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol (1985) 2005; 98(4):1154-62.
Meckel Y, Eliakim A, Seraev M, Zaldivar F, Cooper DM, Sagiv M, et al. The effect of a brief sprint interval exercise on growth factors and inflammatory mediators. J Strength Cond Res 2009; 23(1):225-30.
Ostrowski K, Hermann C, Bangash A, Schjerling P, Nielsen JN, Pedersen BK. A trauma-like elevation of plasma cytokines in humans in response to treadmill running. J Physiol 1998; 513 (Pt 3):889-94.
Baumann H, Gauldie J. The acute phase response. Immunol Today 1994; 15(2):74-80.
Shek PN, Shephard RJ. Physical exercise as a human model of limited inflammatory response. Canadian Journal of Physiology and Pharmacology 1998; 76(5):589-97.
Hovanloo F, Arefirad T, Ahmadizad S. Effects of sprint interval and continuous endurance training on serum levels of inflammatory biomarkers. J Diabetes Metab Disord 2013; 12(1):22.
Suzuki K, Nakaji S, Yamada M, Liu Q, Kurakake S, Okamura N, et al. Impact of a competitive marathon race on systemic cytokine and neutrophil responses. Med Sci Sports Exerc 2003; 35(2):348-55.
Zwetsloot KA, John CS, Lawrence MM, Battista RA, Shanely RA. High-intensity interval training induces a modest systemic inflammatory response in active, young men. J Inflamm Res 2014; 7:9-17.
Ganter U, Arcone R, Toniatti C, Morrone G, Ciliberto G. Dual control of C-reactive protein gene expression by interleukin-1 and interleukin-6. EMBO J 1989; 8(12):3773-9.
Coyle EF. Very intense exercise-training is extremely potent and time efficient: a reminder. J Appl Physiol (1985) 2005; 98(6):1983-4.
Gibala MJ, Little JP, van Essen M, Wilkin GP, Burgomaster KA, Safdar A, et al. Short-term sprint interval versus traditional endurance training: similar initial adaptations in human skeletal muscle and exercise performance. J Physiol 2006; 575(Pt 3):901-11.
Gaesser GA, Angadi SS. High-intensity interval training for health and fitness: can less be more? J Appl Physiol (1985) 2011; 111(6):1540-1.
Robson-Ansley PJ, Blannin A, Gleeson M. Elevated plasma interleukin-6 levels in trained male triathletes following an acute period of intense interval training. Eur J Appl Physiol 2007; 99(4):353-60.
Cha YS. Effects of L-carnitine on obesity, diabetes, and as an ergogenic aid. Asia Pac J Clin Nutr 2008; 17 (Suppl 1):306-8.
Eskandari HG, Cimen MY, Tamer L, Kanik A, Atik U. Short term effects of L-carnitine on serum lipids in STZ-induced diabetic rats. Diabetes Res Clin Pract 2004; 66(2):129-32.
Carnevali LC Jr, Eder R, Lira FS, Lima WP, Gonçalves DC, Zanchi NE, et al. Effects of high-intensity intermittent training on carnitine palmitoyl transferase activity in the gastrocnemius muscle of rats. Braz J Med Biol Res 2012; 45(8):777-83.
Holloway TM, Bloemberg D, da Silva ML, Quadrilatero J, Spriet LL. High-intensity interval and endurance training are associated with divergent skeletal muscle adaptations in a rodent model of hypertension. Am J Physiol Regul Integr Comp Physiol 2015; 308(11):R927-34.
Fallahi AA, Shekarfroush S, Rahimi M, Jalali A, Khoshbaten A. Alteration in cardiac uncoupling proteins and eNOS gene expression following high-intensity interval training in favor of increasing mechanical efficiency. Iran J Basic Med Sci 2016; 19(3):258-64.
Nayebifar S, Afzalpour ME, Kazemi T, Eivary SH, Mogharnasi M. The effect of a 10-week high-intensity interval training and ginger consumption on inflammatory indices contributing to atherosclerosis in overweight women. J Res Med Sci 2016; 21:116.
Kaspar F, Jelinek HF, Perkins S, Al-Aubaidy HA, deJong B, Butkowski E. Acute-phase inflammatory response to single-bout hiit and endurance training: a comparative study. Mediators Inflamm 2016; 2016:5474837.
de Souza DC, Matos VA, Dos Santos VO, Medeiros IF, Marinho CS, Nascimento PR, et al. Effects of high-intensity interval and moderate-intensity continuous exercise on inflammatory, leptin, IgA, and lipid peroxidation responses in obese males. Front Physiol 2018; 9:567. 
Gerosa-Neto J, Antunes BM, Campos EZ, Rodrigues J, Ferrari GD, Rosa Neto JC, et al. Impact of long-term high-intensity interval and moderate-intensity continuous training on subclinical inflammation in overweight/obese adults. J Exerc Rehabil 2016; 12(6):575-80.
Hadiono, Kushartanti BW.High Intensity Interval Training (HIIT) and Moderate Intensity Training (MIT) Against TNF-α and IL-6 levels In Rats. 2nd International Conference on Sports Sciences and Health. Paris: Atlantis Press; 2018. p. 87-90.
Masoumi-Ardakani Y, Aminizadeh S, Fallah H, Shahouzehi B. L-Carnitine different doses affect serum and pancreas tissue antioxidative defense and histopathology in STZ-induced diabetic rats. Biologia 2020; 75(1).
Sobhani V, Mirdar S, Arabzadeh E, Hamidian G, Mohammadi F. High-intensity interval training-induced inflammation and airway narrowing of the lung parenchyma in male maturing rats. Comparative Clinical Pathology 2018; 27(3):577-82.
Karim S, Adams DH, Lalor PF. Hepatic expression and cellular distribution of the glucose transporter family. World J Gastroenterol 2012; 18(46):6771-81.
Holloszy JO, Coyle EF. Adaptations of skeletal muscle to endurance exercise and their metabolic consequences. J Appl Physiol Respir Environ Exerc Physiol 1984; 56(4):831-8.
Prabhu SD. Cytokine-induced modulation of cardiac function. Circ Res 2004; 95(12):1140-53.
Amanollahi N, Tartibian B, Gholami F. Effect of high intensity interval training and honey consumption on some inflammatory indices in sedentary subjects. Int J Health Stud 2020; 6(2):1-5.
Khademi Y, Hosseini SA, Dana F, Hamidi A, Azadmanesh M, Pirouz M. Effect of high intensity interval training with flaxseed on Interleukin-1 Beta and Lipocalin-2 gene expressions in the heart tissue of rats. J Arch Mil Med 2018; 6(4):e83888.
Adiyani P, Abbassi Daloii A, Shadmehri S. The effect of endurance training and L-Carnitine consumption on TNF-a and IL-1β gene expression of heart tissue in wistar male rats following anabolic steroid consumption (Boldenone). Journal of Fasa University of Medical Sciences 2019; 9(4):1903-12. [In Persian].