Isoenzyme Characterization of Trichomonas vaginalis Isolated from HIV Patients in Fars and Kerman, Southeast Iran

Document Type : Original Article


1 Instructor, Department of Parasitology and Mycology‚ School of Medicine‚ Shiraz University of Medical Sciences, Shiraz‚ Iran

2 Associate Professor, Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran

3 Assistant Professor, Department of Parasitology and Mycology‚ School of Medicine‚ Kerman University of Medical Sciences, Kerman‚ Iran

4 Assistant Professor, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran

5 Professor, Basic Sciences in Infectious Diseases Research Center‚ Shiraz University of Medical Sciences, Shiraz‚ Iran



Background: Trichomonas vaginalis is an anaerobic flagellated protozoan which is responsible for human urogenital infections. Several zymodemes of T. vaginalis have been reported from various parts of the worlds on the basis of isoenzyme patterns. This study was conducted to characterize the isolated organisms of T. vaginalis from HIV patients using isoenzyme electrophoresis in Fars and Kerman provinces, southeast Iran.
Methods: Eighteen mass cultivated isolates of T. vaginalis in the modified TYI-S-33 medium were analyzed using isoenzyme electrophoresis. Polyacrylamide gel electrophoresis (PAGE) of five different enzyme systems were used to characterize T. vaginalis isolates: (i) Glucose-6-phosphate dehydrogenase (G6PD), (ii) Glucose phosphate isomerase (GPI), (iii) Malate dehydrogenase (MDH), (iv) Malic enzyme (ME), and (v) Phosphoglucomutase (PGM). 
Results: MDH, GPI, PGM, and ME enzyme systems showed a homogeneity and detected an identical enzyme pattern in all isolates. Meanwhile, G6PD revealed two different enzyme patterns. The isoenzyme electrophoretic profiles divided 18 T. vaginalis isolates into two zymodemes. Zymodeme 1 contained Shiraz isolates and zymodeme 2 contained Kerman isolates.
Conclusion:The polymorphism of Iranian human isolates of T. vaginalis could be assessed by biochemical study using appropriate enzyme systems. Isoenzyme analysis is a promising method for the characterization of T. vaginalis. New molecular studies with increased number of enzyme loci and genetic markers are suggested to classify more zymodemes of Trichomonas in Iran.


Johnston VJ, Mabey DC. Global epidemiology and control of Trichomonas vaginalis. Curr Opin Infect Dis 2008; 21(1):56-64.
Kashan ZF, Arbabi M, Delavari M, Hooshyar H, Taghizadeh M, Joneydy Z. Effect of Verbascum thapsus ethanol extract on induction of apoptosis in Trichomonas vaginalis in vitro. Infect Disord Drug Targets 2015; 15(2):125-30.
Arbabi M, Delavari M, Fakhrieh-Kashan Z, Hooshyar H. Review of Trichomonas vaginalis in Iran, based on epidemiological situation. J Reprod Infertil 2018; 19(2):82-8.
Hezarjaribi HZ, Fakhar M, Shokri A, Teshnizi SH, Sadough A, Taghavi M. Trichomonas vaginalis infection among Iranian general population of women: a systematic review and meta-analysis. Parasitol Res 2015; 114(4):1291-300.
Meade JC, Carlton JM. Genetic diversity in Trichomonas vaginalis. Sex Transm Infect 2013; 89(6):444-8.
Secor WE, Meites E, Starr MC, Workowski KA. Neglected parasitic infections in the United States: trichomoniasis. Am J Trop Med Hyg 2014; 90(5):800-4.
Thompson RC, Meloni BP. Molecular variation in Giardia. Acta Trop 1993; 53(3-4):167-84.
Hatam GR, Hosseini SM, Ardehali S. Isoenzyme studies in characterization of Leishmania isolated in Iran. Iran J Med Sci 1999; 24(1-Jan):8-13.
Meloni B, Lymbery AJ, Thompson RC. Genetic characterization of isolates of Giardia duodenalis by enzyme electrophoresis: implications for reproductive biology, population structure, taxonomy, and epidemiology. J Parasitol 1995; 81(3):368-83.
Miles M, Lainson R, Shaw J, Povoa M, De Souza A. Leishmaniasis in Brazil: XV. Biochemical distinction of Leishmania mexicana amazonensis, L. braziliensis braziliensis and L. braziliensis guyanensis-aetiological agents of cutaneous leishmaniasis in the Amazon Basin of Brazil. Trans R Soc Trop Med Hyg 1981; 75(4):524-9.
Ebert F. Further isoenzymatic studies on Trypanosoma cruzi stocks from Brazil, Colombia and Chile. Trop Med Parasit 1985; 36(2):85-7.
Darde ML, Bouteille B, Pestre-Alexandre M. Isoenzymic characterization of seven strains of Toxoplasma gondii by isoelectrofocusing in polyacrylamide gels. Am J Trop Med Hyg 1988; 39(6):551-8.
Sargeaunt PG, Baveja UK, Nanda R, Anand BS. Influence of geographical factors in the distribution of pathogenic zymodemes of Entamoeba histolytica: identification of zymodeme XIV in India. Trans R Soc Trop Med Hyg 1984; 78(1):96-101.
Homan WL, Van Enckevort FV, Limper L, Van Eys GJ, Schoone GJ, Kasprzak W, et al. Comparison of Giardia isolates from different laboratories by isoenzyme analysis and recombinant DNA probes. Parasitol Res 1992; 78(4):316-23.
Rayani M, Unyah NZ, Vafafar A, Hatam GR. Isoenzyme profiles and phylogenetic analysis of Giardia duodenalis isolates from Iranian patients. Environ Sci Pollut Res 2020; 27(32):40652-63.
Keister DB. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 1983; 77(4):487-8.
Clark CG, Diamond LS. Methods for cultivation of luminal parasitic protists of clinical importance. Clin Microbiol Rev 2002; 15(3):329-41.
Hatam GR, Bahrami S, Razavi SM, Oryan A. Isoenzyme and ultrastructural characterization of Leishmania tropica axenic amastigotes and promastigotes. Parasitol Res 2013; 112(2):643-8.
Hatam GR, Riyad M, Bichichi M, Hejazi SH, Guessous-Idrissi N, Ardehali S. Isoenzyme characterization of iranian leishmania isolates from cutaneous leishmaniasis. Iran J Sci Technol Trans A Sci 2005; 29(1):65-70.
Evans D. Handbook on Isolation, Characterization and Cryopreservation of Leishmania. Geneva: WHO; 1989.
Chýle M, Štěpán J, Chýle P, Patočka F. Some enzyme and isoenzyme activities in Trichomonas vaginalis. Folia Microbiol 1971; 16(2):142-3.
Soliman MA, Ackers JP, Catterall RD. Isoenzyme characterisation of Trichomonas vaginalis. Br J Vener Dis 1982; 58(4):250-6.
Coombs GH, North MJ. An analysis of the proteinases of Trichomonas vaginalis by polyacrylamide gel electrophoresis. Parasitology 1983; 86(Pt 1):1-6.
Gradus MS, Matthews HM. Electrophoretic analysis of soluble proteins and esterase, superoxide dismutase and acid phosphatase isoenzymes of members of the protozoan family trichomonadidae. Comp Biochem Physiol B 1985; 81(1):229-33.
Nadler SA, Honigberg BM. Genetic differentiation and biochemical polymorphism among trichomonads. J Parasitol 1988; 74(5):797-804.
Proctor EM, Naaykens W, Wong Q, Bowie WR. Isoenzyme patterns of isolates of Trichomonas vaginalis from Vancouver. Sex Transm Dis 1988; 15(4):181-5.
Vohra H, Sharma P, Sofi BA, Gupta I, Ganguly NK, Mahajan RC, et al. Correlation of zymodeme patterns, virulence & drug sensitivity of Trichomonas vaginalis isolates from women. Indian J Med Res 1991; 93:37-9.
Azab ME, Salem SA, Abd el Ghaffar FM, el Sherif EA, Habib KS, Habib FS. Characterization of Egyptian isolates of Trichomonas vaginalis: I. Serotyping. J Egypt Soc Parasitol 1992; 22(3):775-82.
Yuan LJ, Gao XZ. [Isoenzyme analysis on different isolates of Trichomonas vaginalis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 2003; 21(2):102-5. [In Chinese].