Genetic Association of ADIPOQ Gene Variant (rs822396) with Type 2 Diabetes in Iranian Patients

Document Type : Short Communication

Authors

1 Departments of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran

2 Department of Genetic, Faculty of Biological Science, Tehran North Branch, Islamic Azad University, Tehran, Iran

3 Department of Medical, Alborz Medical University, Karaj, Iran

4 Fazeli-Sanati Genetics Laboratory, Tehran, Iran

5 Professor, Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Abstract

Background: Genetic and environmental factors influence serum adiponectin and may contribute to the risk of metabolic syndrome and type 2 diabetes (T2D). There are many studies conducted to investigate the association between different polymorphisms of the ADI­POQ geneand T2D risk in all around the world. Therefore, the present study was conducted to investigate the association between adiponectin gene (ADIPOQ) polymorphism (rs822396) and the risk of T2D, which has not been studied yet in the Iranian patients.
Methods: Selected SNP was genotyped in 40 T2D patients and 40 controls by PCR amplification and direct Sanger sequencing. Fisher's exact test and Chi2 test were used to estimate the risk of T2D associated with the selected SNP, and genotypic and allelic distributions were compared between the case and control groups.
Results: It was revealed that there were no significant differences in the distribution of genotypes and allele frequencies of rs822396 between Iranian patients with T2D and controls.
Conclusion: According to the results, -3971 A/G polymorphism is unlikely to be involved in the susceptibility to or the severity of T2D in Iranian patients. Further large prospective studies are required to confirm these findings.

Keywords

References

  1. Zhao N, Li N, Zhang S, Ma Q, Ma C, Yang X, et al. Associations between two common single nucleotide polymorphisms (rs2241766 and rs1501299) of ADIPOQ gene and coronary artery disease in type 2 diabetic patients: a systematic review and meta-analysis. Oncotarget 2017; 8(31):51994-2005.
  2. Chaikhiandee S, Phonrat B, Tungtrongchitr A, Suriyaprom K, Chuengsamarn S, Uttamachai C, et al. Adipoq polymorphisms among thais with pre-diabetes. Southeast Asian J Trop Med Public Health 2016; 47(6):1306-14.
  3. Chu H, Wang M, Zhong D, Shi D, Ma L, Tong N, et al. AdipoQ polymorphisms are associated with type 2 diabetes mellitus: a meta‐analysis study. Diabetes Metab Res Rev2013; 29(7):532-45.
  4. Zhang H, Pollin TI. Epigenetics variation and pathogenesis in diabetes. Curr Diab Rep 2018; 18(11):121.
  5. Misra A, Sattar N, Tandon N, Shrivastava U, Vikram NK, Khunti K, et al. Clinical management of type 2 diabetes in south Asia. Lancet Diabetes Endocrinol 2018; 6(12):979-91.
  6. Fuchsberger C, Flannick J, Teslovich TM, Mahajan A, Agarwala V, Gaulton KJ, et al. The genetic architecture of type 2 diabetes. Nature 2016; 536(7614):41-7.
  7. Kyriakou T, Collins LJ, Spencer-Jones NJ, Malcolm C, Wang X, Snieder H, et al. Adiponectin gene ADIPOQ SNP associations with serum adiponectin in two female populations and effects of SNPs on promoter activity. J Hum Genet 2008; 53(8):718-27.
  8. Ahima RS, Flier JS. Adipose tissue as an endocrine organ. Trends Endocrinol Metab 2000;11(8):327-32.
  9. Duncan BB, Schmidt MI, Pankow JS, Bang H, Couper D, Ballantyne CM, et al. Adiponectin and the development of type 2 diabetes: the atherosclerosis risk in communities study. Diabetes 2004; 53(9):2473-8.
  10. Marseglia L, Manti S, D'Angelo G, Nicotera A, Parisi E, Di Rosa G, et al. Oxidative stress in obesity: a critical component in human diseases. Int J Mol Sci 2014; 16(1):378-400.
  11. Kitamoto A, Kitamoto T, So R, Matsuo T, Nakata Y, Hyogo H, et al. ADIPOQ polymorphisms are associated with insulin resistance in Japanese women. Endocr J 2015; 62(6):513-21.
  12. Ramya K, Ayyappa KA, Ghosh S, Mohan V, Radha V. Genetic association of ADIPOQ gene variants with type 2 diabetes, obesity and serum adiponectin levels in south Indian population. Gene 2013; 532(2):253-62.
  13. Warodomwichit D, Shen J, Arnett DK, Tsai MY, Kabagambe EK, Peacock JM, et al. ADIPOQ polymorphisms, monounsaturated fatty acids, and obesity risk: the GOLDN study. Obesity (Silver Spring) 2009; 17(3):510-7.
  14. Matsuzawa Y. Therapy insight: adipocytokines in metabolic syndrome and related cardiovascular disease. Nat Clin Pract Cardiovasc Med 2006; 3(1):35-42.
  15. Hotta K, Funahashi T, Bodkin NL, Ortmeyer HK, Arita Y, Hansen BC, et al. Circulating concentrations of the adipocyte protein adiponectin are decreased in parallel with reduced insulin sensitivity during the progression to type 2 diabetes in rhesus monkeys. Diabetes 2001; 50(5):1126-33.
  16. Jee SH, Sull JW, Lee JE, Shin C, Park J, Kimm H, et al. Adiponectin concentrations: a genome-wide association study. Am J Hum Genet 2010; 87(4):545-52.
  17. Dendana M, Bahia W, Finan RR, Al-Mutawa M, Almawi WY. Association of adiponectin gene variants with idiopathic recurrent miscarriage according to obesity status: a case-control study. J Transl Med 2018; 16(1):76.
  18. Biswas D, Vettriselvi V, Choudhury J, Jothimalar R. Adiponectin gene polymorphism and its association with type 2 diabetes mellitus. Indian J Clin Biochem 2011; 26(2):172-7.
  19. Kang ES, Magkos F, Kim BS, Zhai R, Su L, Kim YS, et al. Variants of the adiponectin and adiponectin receptor-1 genes and posttransplantation diabetes mellitus in renal allograft recipients. J Clin Endocrinol Metab 2012; 97(1):E129-35.
  20. Mtiraoui N, Ezzidi I, Turki A, Chaieb A, Mahjoub T, Almawi WY. Single-nucleotide polymorphisms and haplotypes in the adiponectin gene contribute to the genetic risk for type 2 diabetes in Tunisian Arabs. Diabetes Res Clin Pract 2012; 97(2):290-7.
  21. Tso AW, Sham PC, Wat NM, Xu A, Cheung BM, Rong R, et al. Polymorphisms of the gene encoding adiponectin and glycaemic outcome of Chinese subjects with impaired glucose tolerance: a 5-year follow-up study. Diabetologia 2006; 49(8):1806-15.
  22. Wassel CL, Pankow JS, Jacobs DR Jr, Steffes MW, Li N, Schreiner PJ. Variants in the adiponectin gene and serum adiponectin: the Coronary Artery Development in Young Adults (CARDIA) Study. Obesity (Silver Spring) 2010; 18(12):2333-8.
  23. Mofarrah M, Ziaee S, Pilehvar-Soltanahmadi Y, Zarghami F, Boroumand M, Zarghami N. Association of KALRN, ADIPOQ, and FTO gene polymorphism in type 2 diabetic patients with coronary artery disease: possible predisposing markers. Coron Artery Dis 2016; 27(6):490-6.
  24. Erfanian S, Moradzadeh M, Solhjoo K, Jahromi AS. Data describing the association between rs266729 polymorphism inadiponectin promoter gene and Type 2 Diabetes Mellitus. Data Brief 2016; 9:1138-40.
  25. Namvaran F, Rahimi-Moghaddam P, Azarpira N, Dabbaghmanesh MH. Polymorphism of adiponectin (45T/G) and adiponectin receptor-2 (795G/A) in an Iranian population: relation with insulin resistance and response to treatment with pioglitazone in patients with type 2 diabetes mellitus. Mol Biol Rep 2012; 39(5):5511-8.

 

Journal of Kerman University of Medical Sciences
Volume 28, Issue 2
March and April 2021
Pages 212-218

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