Document Type : Original Article

Authors

1 Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute & Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

2 2. Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

3 Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

4 Department of Medical Parasitology and Mycology, Faculty of public health, Tehran University of Medical Sciences, Tehran, Iran

5 Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran

Abstract

Background: Toxoplasma gondii is one of the most prevalent zoonotic opportunistic parasitic infections in the world. The aim of this study was to examine chronic and acute toxoplasmosis molecularly and serologically in Hemodialysis patients.
Methods: A total of 238 serum samples of hemodialysis patients with chronic renal disease (119 samples) as case group and healthy individuals (119 samples) as control group were enrolled in Zahedan City, southeastern Iran. The molecular and serological detection of toxoplasmosis were conducted on all samples using nested-PCR and ELISA.
Results: The prevalence rates of anti-T. gondii IgM and IgG and T. gondii DNA in the case group were respectively 0%, 44.5% and 29.4% and the corresponding values in the control group were 0.8%,23.5% and 2.52 % respectively (p≥0.05).
Conclusion: In conclusion, preliminary Toxoplasma gondii infection screening is required using serological techniques, particularly in hemodialysis patients who are frequently exposed to hemodialysis so as to stop infection dissemination.

Keywords

  1. Karami MF, Rafiei A, Raiesi O, Getso M, Akhlaghi E, Jalali P, et al. The Relation between toxocariasis and toxoplasmosis co-infection and the presence of Rheumatoid Factor (RF) in people with hydatidosis in Southwestern Iran, from 2013 to 2018. J Parasit Dis 2019; 43(3):379-84. doi: 10.1007/s12639-019-01101-x
  2. Eskandarian A, Jahani S, Hejazi H, Yousefi H, Raissi V. Investigation of Toxoplasma gondii infection in cutaneous leishmaniasis patients of the Isfahan province. Int J Infect 2017; 4(2):e40260. doi: 10.5812/iji.40260.
  3. Sekandarpour S, Jafari Modrek M, Shafiei R, Mohammadiha A, Etemadi S, Mirahmadi H. Determination of parasitic burden in the brain tissue of infected mice in acute toxoplasmosis after treatment by fluconazole combined with sulfadiazine and pyrimethamine. Eur J Med Res 2021; 26:65.
  4. Soltani S, Khademvatan S, Saki J, Shahbazian H. Detection of toxoplasmosis in renal transplant recipients by ELISA and PCR methods in Ahvaz, south-west of Iran. Jundishapur J Microbiol 2013; 6(9):e7642. doi : 10.5812/jjm.7642.
  5. Zarean M, Shafiei R, Gholami M, Fata A, Balaghaleh MR, Karimi A, et al. Seroprevalence of anti–Toxoplasma gondii antibodies in healthy voluntary blood donors from Mashhad City, Iran. Arch Iran Med 2017; 20(7): 441-445.
  6. Zarean M, Shafiei R, Gholami M, Fata A, Rahmati Balaghaleh M, Kariminik A, et al. Seroprevalence of anti-Toxoplasma gondii antibodies in healthy voluntary blood donors from Mashhad city, Iran. Arch Iran Med 2017; 20(7):441-5.
  7. Mirahmadi H, Nozari B, Raissi V, Alijani E, Etemadi S. Frequency and parasite load of Toxoplasma gondii in hemodialysis patients based on RE gene by real-time PCR. Gene Reports 2021; 23:101145. doi: 10.1016/j.genrep.2021.101145.
  8. Dalimi A, Abdoli A. Latent toxoplasmosis and human. Iran J Parasitol 2012; 7(1):1-17.
  9. Mirahmadi H, Hasanzadeh R, Raeesi HM, Fallahi S, Shahraki MK, Badirzadeh A. Loop-mediated isothermal amplification (Lamp) assay to detect toxoplasmosis in schizophrenia patients. Iran J Parasitol 2020;15(3):299-306.
  10. Jafari Modrek M, Hasanzadeh R, Foroutan M, Mirahmadi H, Rahmati-Balaghaleh M, Zarean M. Seroprevalence and molecular evaluation of Toxoplasma gondii in Schizophrenic patients hospitalized in Sistan and Baluchestan province, Southeast of Iran. Trop Biomed 2019; 36(2):422-9.
  11. Keshavarz H, Mamishi S, Daneshvar H. The prevalence of Toxoplasma infection in hospitalized patients in selected hospitals of Kerman. J Kerman Univ Medical Sci 2000; 7(3):129-36. [In Persian].
  12. Rezazadeh Varaghchi J, Ahmadi Shadmehri A, Ahmadi Shadmehri A. Seroepidemiology of toxoplasmosis in women referred to Birjand Milad genetic counseling center during 2011-2013. J Kerman Univ Medical Sci 2015; 22(5):524-32. [In Persian].
  13. Kamyabi Z, Atapour Investigation of the prevalence of Toxoplasma antibodies in women during marriage consultation in Kerman city. J Kerman Univ Medical Sci 1999; 6(3):127-33. [In Persian].
  14. Shafiei R, Riazi Z, Sarvghad M, Galian Sharifdini M, Mahmoodzadeh A, Hajia M. Prevalence of IgG and IgM anti-toxoplasma gondii Antibodies in HIV positive patients in Northeast of Iran. Iran J Pathol 2011; 6(2):68-72.
  15. Arab-Mazar Z, Fallahi S, Koochaki A, Mirahmadi H, Seyyed Tabaei SJ. Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein. Iran J Microbiol 2016; 8(5):331-37.
  16. Foroutan M, Rostami A, Majidiani H, Riahi SM, Khazaei S, Badri M, et al. A systematic review and meta-analysis of the prevalence of toxoplasmosis in hemodialysis patients in Iran. Epidemiol Health 2018; 40:e2018016. doi: 10.4178/epih.e2018016.
  17. Raissi V, Bayat F, Taghipour A, Raiesi O, Ibrahim A, Getso M, et al. Seroepidemiology and risk factors of toxoplasmosis among children age ranged from 1 to 14 years referred to medical diagnostic laboratories in Southeast Iran. Clin Epidemiol Glob Health 2020; 8(2):595-9. doi: 10.1016/j.cegh.2019.12.009.
  18. Ebrahim Zadeh A, Bamedi T, Etemadi S, Shahrakipour M, Saryazdipour K. Toxoplasmosis as a complication of transfusion in hemodialysis patients. Iran J Ped Hematol Oncol 2014; 4(1):22-5.
  19. Mousavi M, Saravani R, Jafari Modrek M, Shahrakipour M, Sekandarpour S. Detection of Toxoplasma gondii in diabetic patients using the Nested PCR assay via RE and B1 genes. Jundishapur J Microbiol 2016; 9(2):e29493. doi: 10.5812/jjm.29493.
  20. SakiJ, Khademvatan S, Soltani S, Shahbazian Detection of toxoplasmosis in patients with end-stage renal disease by enzyme-linked immunosorbent assay and polymerase chain reaction methods. Parasitol Res 2013; 112(1):163-8. doi: 10.1007/s00436-012-3120-6.
  21. Bayani M, Mostafazadeh A, Oliaee F, Kalantari N. The prevalence of Toxoplasma gondii in hemodialysis patients. Iran Red Crescent Med J 2013; 15(10):e5225. doi: 10.5812/ircmj.5225.
  22. Rezavand B, Mahmoodzadeh Poornaki A, Rahim Mokhtari K, Mohammad A, Andalibian A, Abdi J. Identification and determination of the prevalence of Toxoplasma gondii in patients with chronic renal failure by ELISA and PCR. Asian Pac J Trop Dis 2016; 6(5):347-9. Doi: 10.1016/S2222-1808(15)61044-1.
  23. Solhjoo K, Sotoodeh Jahromi A, Parnian-Rad A. Anti-Toxoplasma gondii antibodies in haemodialysis patients. Am J Infect Dis 2010; 6(1):13-7. doi: 10.3844/ajidsp.2010.13.17.
  24. Maraghi S, Yadyad MJ, Sheikhi M, Shamakhteh F, Latifi SM. Study the anti-toxoplasma antibodies (IgG and IgM) in hemodialysis patients of Abadan and Khoramshahr cities southwest Iran in 2011 using ELISA. Jundishapur J Microbiol 2013; 6(7):e7113. doi:10.5812/jjm.7113.
  25. Kazemi F, Fallahizadeh S, Feizhaddad MH. Seroepidemiological study of toxoplasmosis in hemodialysis patients of Ahvaz, Southwest of Iran. Int J BioMed Public Health 2018; 1(1):12-6. doi: 10.22631/ijbmph.2018.55340.
  26. Hamidi F, Etemadi J, Ghabouli Mehrabani N, Mahami Oskouei M, Motavalli R, Ardalan MR. Comparison of Toxoplasma gondii seropositivity in hemodialysis and peritoneal dialysis patients. Journal of Coastal Life Medicine 2015; 3(8):621-2. doi: https://doi.org/10.12980/JCLM.3.2015J5-76.
  27. Khalili B, Mortezaei S, Fazeli M. The comparison of anti-Toxoplasma antibody (IgM, IgG) in hemodialysed patients and those undergoing chemoterapy with healthy blood donor, shahr-E-kord, 1392. Iran J Parasitol 2015; 10(Suppl 1):311-311.
  28. Rasti S, Hassanzadeh M, Soliemani A, Hooshyar H, Mousavi SG, Nikoueinejad H, et al. Serological and molecular survey of toxoplasmosis in renal transplant recipients and hemodialysis patients in Kashan and Qom regions, central Iran. Ren Fail 2016; 38(9):970-3. doi: 10.3109/0886022X.2016.1172940.
  29. Seyyedpour SH, Afshar P, Barzegarnejad A, Kalhori S, Agah R. Evaluation of anti-Toxoplasma gondii antibodies in hemodialysis patients with chronic kidney disease in Sari, Iran. Nephrourol Mon 2016; 8(6):e40182. doi: 10.5812/numonthly.40182.
  30. Dorri M, Dabirzadeh M, Maroufi Y, Afshari M, Badri Chokamy M. Prevalence of anti-Toxoplasma IgG and IgM in hemodialysis patients comparing to healthy individuals in Sistan area, Iran. J Nephropharmacol 2017; 6(2):106-9. doi: 10.15171/npj.2017.13.
  31. Aufy SM, Mahgoub AM, Saadi MG, Adel Elmallawany M. Serological detection of Toxoplasma gondii in chronic renal failure patients and renal transplant recipients. J Egypt Soc Parasitol 2009; 36(4):943-50.
  32. Ocak S, Duran N, Eskiocak AF, Aytac H. Anti-Toxoplasma gondii antibodies in hemodialysis patients receiving long-term hemodialysis therapy in Turkey. Saudi Med J 2005; 26(9):1378-82.
  33. Abbas MM, Zaki M, Afify NA. Prevalence of Toxoplasma gondii and Cytomegalovirus antibodies in patients with chronic renal failure. J Egypt Soc Parasitol 1996; 26(3):671-6.
  34. Esposito R, Orlando G, Inzoli C, Calello G, Moroni M, Rivolta E, et al. Asyptomatic parasitic infections in renal transplant recipients and in haemodialysis patients. Boll Ist Sieroter Milan 1987; 66(3):210-3.
  35. Tong DS, Yang J, Xu GX, Shen GQ. Serological investigation on Toxoplasma gondii infection in dialysis patients with renal insufficiency. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi 2011; 23(2):144-53. [In Chinese].