Zinc Finger Protein 510 Levels in the Saliva of Patients with Oral Lichen Planus Compared with Healthy Controls

Document Type : Original Article


1 Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

4 Dentist, Mashhad, Iran

5 Department of Oral Medicine and Radiology, New Horizon Dental College and Research Institute Bilaspur, India



Background: The aim of this study was to evaluate the concentration of zinc finger protein 510 (ZNF510) in the saliva of patients with oral lichen planus and healthy individuals in 2019.
Methods: This cross-sectional analytical study was performed on 24 patients with oral lichen planus and 25 healthy individuals referred to the School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran, between June and October 2019. In the case group, the severity of lesions was determined according to the Thongprasom index. Unstimulated saliva was collected from the subjects and the samples were examined for the presence of the ZNF510 protein using ELISA method. The data were statistically analyzed through SPSS 23. For data analysis, Kolmogorov-Smirnov test, the independent t-test, the independent samples Kruskal-Wallis test, and one-way analysis of variance were used.
Results: This study included 32 females (65.3%), and 17 males (34.7%). The subjects’ age range was between 23-70 years and the mean age of them was 46.26 ± 10.90 years. The mean ZNF510 (ppm) in the case group was 86.12 ± 34.88, while in the control group, it was 46.43 ± 23.32. The two groups were significantly different in terms of the mean ZNF510 (P <0.001). In patients with non-keratotic lichen planus, the mean ZNF510 was significantly higher than that in those with keratotic lesions (P = 0.028). Moreover, in patients with oral lichen planus, the severity of lesions according to the Thongprasom index was significantly and directly related to the ZNF510 concentration (P = 0.002).
Conclusion: The concentration of ZNF510 protein in the saliva can be a good indicator for assessing the severity of oral lichen planus lesions and its diagnosis. However, its clinical application is possible only if extensive prospective studies are performed.


  • Alrashdan MS, Cirillo N, McCullough M. Oral lichen planus: a literature review and update. Arch Dermatol Res 2016; 308(8):539-51. doi: 10.1007/s00403-016-1667-2.
  • Chiang CP, Yu-Fong Chang J, Wang YP, Wu YH, Lu SY, Sun A. Oral lichen planus - Differential diagnoses, serum autoantibodies, hematinic deficiencies, and management. J Formos Med Assoc 2018; 117(9):756-65. doi: 10.1016/j.jfma.2018.01.021.
  • Mutafchieva MZ, Draganova-Filipova MN, Zagorchev PI, Tomov GT. Oral lichen planus - known and unknown: a review. Folia Med (Plovdiv) 2018; 60(4):528-35. doi: 10.2478/folmed-2018-0017.
  • Talungchit S, Buajeeb W, Lerdtripop C, Surarit R, Chairatvit K, Roytrakul S, et al. Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study. BMC Oral Health 2018; 18(1):42. doi: 10.1186/s12903-018-0504-8.
  • Ion DI, Setterfield JF. Oral Lichen Planus. Prim Dent J 2016; 5(1):40-4. doi: 10.1177/205016841600500104.
  • Nosratzehi T. Oral lichen planus: an overview of potential risk factors, biomarkers and treatments. Asian Pac J Cancer Prev 2018; 19(5):1161-7. doi: 10.22034/APJCP.2018.19.5.1161.
  • Lopez-Jornet P, Cayuela CA, Tvarijonaviciute A, Parra-Perez F, Escribano D, Ceron J. Oral lichen planus: salival biomarkers cortisol, immunoglobulin A, adiponectin. J Oral Pathol Med 2016; 45(3):211-7. doi: 10.1111/jop.12345.
  • Rekha VR, Sunil S, Rathy R. Evaluation of oxidative stress markers in oral lichen planus. J Oral Maxillofac Pathol 2017; 21(3):387-93. doi: 10.4103/jomfp.JOMFP_19_17.
  • Jou YJ, Lin CD, Lai CH, Tang CH, Huang SH, Tsai MH, et al. Salivary zinc finger protein 510 peptide as a novel biomarker for detection of oral squamous cell carcinoma in early stages. Clinica Chimica Acta 2011; 412(15-16):1357-65. doi: 10.1016/j.cca.2011.04.004.
  • Ladomery M, Dellaire G. Multifunctional zinc finger proteins in development and disease. Ann Hum Genet 2002; 66(Pt 5-6):331-42. doi: 10.1017/S0003480002001215.
  • Fu M, Blackshear PJ. RNA-binding proteins in immune regulation: a focus on CCCH zinc finger proteins. Nat Rev Immunol 2017; 17(2):130-43. doi: 10.1038/nri.2016.129.
  • Amirchaghmaghi M, Pakfetrat A, Delavarian Z, Ghalavani H, Ghazi A. Evaluation of the efficacy of curcumin in the treatment of oral lichen planus: a randomized controlled trial. J Clin Diagn Res 2016; 10(5):ZC134-7. doi: 10.7860/JCDR/2016/16338.7870.
  • Duffey DC, Eversole LR, Abemayor E. Oral lichen planus and its association with squamous cell carcinoma: an update on pathogenesis and treatment implications. Laryngoscope 1996; 106(3 Pt 1):357-62. doi: 10.1097/00005537-199603000-00022.
  • Thongprasom K, Luangjarmekorn L, Sererat T, Taweesap W. Relative efficacy of fluocinolone acetonide compared with triamcinolone acetonide in treatment of oral lichen planus. J Oral Pathol Med 1992; 21:456-8. doi: 10.1111/j.1600-0714.1992.tb00974.x.
  • Malamud D. Saliva as diagnostic fluid. Dent Clin North Am 2011; 55(1):159-78. doi: 10.1016/j.cden.2010.08.004.
  • West IC. Radicals and oxidative stress in diabetes. Diabet Med 2000; 17(3):171-80. doi: 10.1046/j.1464-5491.2000.00259.x.
  • Lorenzo-Pouso AI, Pérez-Sayáns M, Bravo SB, López-Jornet P, García-Vence M, Alonso-Sampedro M, et al. Protein-based salivary profiles as novel biomarkers for oral diseases. Dis Markers 2018; 2018:6141845. doi: 10.1155/2018/6141845.
  • Gholizadeh, N., Sheykhbahaei, N., Mansourian, A., Amini-Shakib, P., Sadrzadeh-Afshar, M., Fooladvand, S. Lack of association between Estrogen and progesterone receptors and Oral Lichen Planus. Journal of Kerman University of Medical Sciences, 2020; 27(1): 24-35. doi: 10.22062/jkmu.2020.89593
  • Agha-Hosseini, F., Mirzaii-Dizgah, I., Mohebbian, M., Sarookani, M. Vascular Endothelial Growth Factor in Serum and Saliva of Oral Lichen Planus and Oral Squamous Cell Carcinoma Patients. Journal of Kerman University of Medical Sciences, 2018; 25(1): 27-33.
  • Amirchaghmaghi M, Pakfetrat A, Delavarian Z, Ghalavani H, Ghazi A. Evaluation of the Efficacy of Curcumin in the Treatment of Oral Lichen Planus: A Randomized Controlled Trial. J Clin Diagn Res. 2016 May;10(5):ZC134-7. doi: 10.7860/JCDR/2016/16338.7870. Epub 2016 May 1. PMID: 27437348; PMCID: PMC4948524.
  • Lodi G, Carrozzo M, Furness S, Thongprasom K. Interventions for treating oral lichen planus: a systematic review. Br J Dermatol 2012; 166(5):938-47. doi: 10.1111/j.1365-2133.2012.10821.x.
  • Maheswari TN, Venugopal A, Sureshbabu NM, Ramani P. Salivary micro RNA as a potential biomarker in oral potentially malignant disorders: A systematic review. Ci Ji Yi Xue Za Zhi 2018; 30(2):55-60. doi: 10.4103/tcmj.tcmj_114_17.
  • Rezaei Chianeh Y, Prabhu K. Biochemical markers in saliva of patients with oral squamous cell carcinoma. Asian Pac J Trop Dis 2014; 4(Suppl 1):S33-40. doi: 10.1016/S2222-1808(14)60412-6.
  • Humberto JS, Pavanin JV, Rocha MJ, Motta AC. Cytokines, cortisol, and nitric oxide as salivary biomarkers in oral lichen planus: a systematic review. Braz Oral Res 2018; 32:e82. doi: 10.1590/1807-3107bor-2018.vol32.0082.
  • Totan A, Miricescu D, Parlatescu I, Mohora M, Greabu M. Possible salivary and serum biomarkers for oral lichen planus. Biotech Histochem 2015; 90(7):552-8. doi: 10.3109/10520295.2015.1016115.
  • Srinivasan M, Kodumudi KN, Zunt SL. Soluble CD14 and toll-like receptor-2 are potential salivary biomarkers for oral lichen planus and burning mouth syndrome. Clin Immunol 2008; 126(1):31-7. doi: 10.1016/j.clim.2007.08.014.
  • Mizukawa N, Sugiyama K, Ueno T, Mishima K, Takagi S, Sugahara T. Defensin-1, an antimicrobial peptide present in the saliva of patients with oral diseases. Oral Dis 1999; 5(2):139-42. doi: 10.1111/j.1601-0825.1999.tb00078.x.
  • Yang LL, Liu XQ, Liu W, Cheng B, Li MT. Comparative analysis of whole saliva proteomes for the screening of biomarkers for oral lichen planus. Inflamm Res 2006; 55(10):405-7. doi: 10.1007/s00011-006-5145-8.
  • Liu J, Shi Q, Yang S, Wang Q, Xu J, Guo B. The relationship between levels of salivary and serum interleukin-6 and oral lichen planus: A systematic review and meta-analysis. J Am Dent Assoc 2017; 148(10):743-9. doi: 10.1016/j.adaj.2017.05.007.
  • Cassandri M, Smirnov A, Novelli F, Pitolli C, Agostini M, Malewicz M, et al. Zinc-finger proteins in health and disease. Cell Death Discov 2017; 3:17071. doi: 10.1038/cddiscovery.2017.71.
  • Ahuja P. Oral cancer biomarkers-as powerful diagnostic and prognostic tools. Int J Curr Microbiol App Sci 2016; 5(1):209-20.