Document Type : Original Article
Authors
1 Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
2 Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
3 Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Abstract
Background: Liver oxidative metabolism disorder is dependent on several factors that lead to various forms of liver disease like non-alcoholic fatty liver disease. We evaluate the effect of high-intensity interval training (HIIT) in the presence of L-carnitine on the expression of Monocarboxylate transporter 1 (MCT1), peroxisome proliferator-activated receptor-γ coactivator-1α; (PGC-1α), and citrate synthase (Cs) in the liver of male Wistar rats.
Methods: Thirty-two male Wistar rats were randomly divided into 4 groups (n = 8); Control, L-carnitine ( i.p 200 mg/kg/day; 4 weeks), HIIT, and HIIT + L-carnitine( i.p 200 mg/kg/day; 4weeks). HIIT training was performed for 4 weeks (5 days a week), and twenty-four hours after the last training session, animals were anesthetized and their liver tissues were extracted for performing Real time-PCR to measure MCT1, PGC-1α, and CS. Data obtained in different groups were analyzed in SPSS 22 software, and a level of P<0.05 was considered significant.
Results: The MCT1 expression was significantly increased in HIIT and HIIT+L-carnitine groups compared to the control group (P<0.001). The HIIT+L-carnitine group significantly increased PGC-1α expression compared to the control and L-carnitine groups (P<0.001). Also, the HIIT group significantly increased PGC-1α expression compared to the control group (P<0.001). Furthermore, in all groups, CS expression significantly increased compared to the control (P<0.001).
Conclusion: HIIT exercise combined with L-carnitine supplementation increases the expression of MCT1, PGC-1α, and CS in the liver. Therefore, it seems that performing HIIT exercises and taking L-carnitine supplements can prevent the consequences of fat accumulation in the liver.
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