Document Type : Original Article
Authors
1 Department of Cell and Molecular Biology, Faculty of chemistry, University of Kashan, Kashan, Iran
2 Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran
Abstract
Background: Antibiotic treatment is used to inhibit the growth of bacterial infections. However, antibiotic resistance is increasing due to the use of these antibiotics. Plant extracts are natural compounds with unique properties that have medical properties. The current study investigated the effect of Melissa officinalis collected from Marivan, Iran, against urinary tract infection (UTI).
Methods: The plant extract was prepared by soxhlet. The bacterial strains were isolated from UTI patients from Al-Zahra Hospital in Najaf, Iraq. To evaluate the antibacterial property of M. officinalis extract, the Kirby-Bauer disk diffusion method and microdilution method were used to determine the minimal inhibitory concentration (MIC).
Results: According to the inhibition zone results, M. officinalis greatly affected Staphylococcus aureus, Proteus vulgaris, and Klebsiella pneumonia, respectively. The plant extract showed the most antibacterial property for standard and clinical of strain S.aureus with the highest inhibition zone record of 8.4±0.163 and 9±00 mm and MIC 0.09 and 0.06 mg/ml, respectively. Also, M. officinalis extract indicated the antimicrobial effect against the standard and clinical strains of K. pneumonia with the highest inhibition zone record of 8±0.0 and 9±00 mm and MIC 0.06 and 0.06 mg/ml, respectively.
Furthermore, the plant extract showed the most antimicrobial activity on standard and clinical strains of P. vulgaris with the highest record of 7.9±0.23 and 9±00 mm and MIC 0.14 and 0.06 mg/ml, respectively.
Conclusion: M. officinalis extract can be a suitable candidate as an alternative to antibiotics for treating UTIs.
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