Document Type : Original Article
Authors
- Navid Omidifar 1
- Mohammad Hadi Imanieh 2
- farshad kakian 3
- Mohsen Moghadami 4
- Khadijehsadat najib 5
- Sahand Mohammadzadeh 6
- Mohammad Motamedifar 7
1 Clinical education research center, and Department of pathology, School of medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Pediatrics, School of Medicine, Gastroenterohepatology Research Center, Internal Medicine Research Institute, Shiraz University of Medical Sciences, Shiraz, Iran
3 Department of Bacteriology and Virology, School of Medicine, Student Research Committee of Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5 Neonatal research center, Shiraz University Of Medical Sciences, Shiraz, Iran
6 Department of Pathology, Shiraz University of medical science, Shiraz, Iran
7 Shiraz HIV/AIDS Research Center, Institute of Health & Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Background: Bacterial infections are the major causes of death in neonates. Growing antibiotics resistance among bacterial infections is an important concern. We detected bacterial isolates from blood cultures, and their antibiotic resistance pattern was determined in neonatal insensitive care unit (NICU) of a major referral hospital in Shiraz, Southwest of Iran.
Methods: In a four-year period, all neonates admitted to the NICU were evaluated. Blood samples were cultured in BACTEC. We examined 5937 blood cultures from May 2015 to March 2020. Antibacterial sensitivity testing was done by disk diffusion method, following the standard criteria of CLSI.
Results: Out of total 705 Gram positive and negative isolates, the identified Gram-negative bacteria were 61 Acinetobacter (8.7%), 40 Escherichia coli (5.7%), 11 Klebsiella (1.6%), 10 Enterobacter (1.4%), and 6 Pseudomonas (0.9%) isolates. Of Gram positive isolates, Coagulase-positive 359 Staphylococci (51.1%), 108 CoNS (15.4%), 74 Staphylococcus epidermidis (10.2%), 17 Non-hemolytic Streptococci (2.4%), 10 Alpha hemolytic Streptococci (1.4%), and 9 Streptococcus pneumoniae (1.3%) isolates were identified. Among Staphylococcus aureus isolates, 318 (88.5%) isolates were methicillin resistant (MRSA).
Conclusion: In this study, the most common bacteria in the NICU was Coagulase-positive Staphylococci. Also in infants who died Acinetobacter isolates had commonly been isolated.
Keywords
Main Subjects
)