@article { author = {Mohammadi-kechikaghi, Sorayya and HosseinpourFeizi, Mohammad Ali and Pouladi, Nasser and Khalaj-Kondori, Mohammad and Shavali, Mojtaba and Azarfam, Parvin}, title = {Upregulation of HOTAIR Transcript Level in Tumor Tissue of Iranian Women with Breast Cancer}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {382-389}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {Background:Dysregulation of HOX Transcript Antisense Intergenic RNA (HOTAIR) has been linked to the etiopathogenesis of several human cancers. According to epidemiological evidences, the risk of susceptibility to breast cancer varies among different populations. This study was designed to determine the transcriptional level of HOTAIR in tumor cells of breast cancer patients compared to normal marginal cells and to identify if this molecule has the potential to be considered as a biomarker in the prognosis of breast cancer. Methods:A total of 37 patients with breast cancer were recruited. Tumor and matched normal tumor-free margin samples were collected during surgery from each patient. Following total RNA extraction and cDNA synthesis, quantitative analysis was performed by real-time PCR using the SYBR Green PCR Master Mix to determine the transcript level of HOTAIR in samples. Results:It was observed that mRNA expression level of HOTAIR was upregulated in tumor cells compared with normal tumor free marginal cells of breast cancer patients (Fold change= +6.9; p = 0.0001). However, no statistically significant correlations were observed between the mRNA expression level of HOTAIR and clinicopathological manifestations of the patients. Conclusions:The results were in accord with what had previously been reported and therefore, it can be concluded that in Iranian population, too, HOTAIR is upregulated in tumor cells of breast cancer patients and has the potential to be considered as a biomarker for the prognosis of breast cancer.}, keywords = {breast cancer,HOTAIR,mRNA expression,Cancer biomarker}, url = {https://jkmu.kmu.ac.ir/article_83077.html}, eprint = {https://jkmu.kmu.ac.ir/article_83077_32ba557a65b1fd02952d807a7142670a.pdf} } @article { author = {honarvar, zahra and Naderi, Tayebeh and Heydarpour, Masoomeh}, title = {The Prevalence of Pre-invasive and Invasive Lesions of the Cervix in Women with Post Coital Bleeding Referred to Gynecology Oncology Clinic of Afzalipoor Hospital, Kerman from March2015 to March2016}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {390-395}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {Background: Bleeding during or immediately after the intercourse can be seen in women with benign cervical changes such as polyps, ectropion or infection of the cervix. But, sometimes, important diseases such as cancer of the cervix can be associated with post coital bleeding (PCB). The aim of this study was to find the prevalence of microscopic pre-cancerous lesions of the cervix in women with PCB. Methods: In this study, 120 women with PCB were entered and studied by liquid based Pap smear, colposcopy and biopsy. Also, some of them were studied with endocervical brush and the obtained data were analyzed through SPSS software. Results: In this study, 113 of 120 women had normal Pap smear, while 7 ones had abnormal Pap smear and ASCUS was reported in these 7 Pap smear samples. Of 120 studied women, 9 patients (7.5%) had endocervical polyps, 72 patients (60%) had chronic cervicitis, 12 cases (10%) had CIN І, 4 patients (3.3%) had CIN II-III and 2 patients (1.7%) had cervical cancer. Of 18 subjects who had abnormal pathology, 14 patients (77.8%) had normal Pap smear. Conclusion: Since significant percentage of patients with abnormal pathology had normal Pap smears, it is recommended that all women who complain from persistent and untreatable PCB undergo colposcopy and biopsy. Pap smear alone is not enough for triage these patients due to its vast false-negative reports.}, keywords = {Post coital bleeding (PCB),Cervical intra-epithelial neoplasia (CIN),Cervical cancer}, url = {https://jkmu.kmu.ac.ir/article_83078.html}, eprint = {https://jkmu.kmu.ac.ir/article_83078_570e7a82ea0b3c4757bd2dfd321e83e2.pdf} } @article { author = {Shiravand, Babak and Rafinejad, Javad and Enayati, Ahmadali and Bani-Ardalani, Mojgan and Vatandoost, Hassan and Keshavarzi, Davoud and Rafinejad, Atiyeh and Saneidehkordi, Alireza}, title = {Assessing the Susceptibility Status of Cypermethrin resistance in German Cockroaches (Blattella germanica: Blattellidae) to Hydramethylnon Gel Bait}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {396-404}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {Background: The German cockroach (Blatella germanica) is one of the most important vectors of public health interest due to its involvement in the transmission of infectious diseases. Different insecticides are used to control cockroaches; however, resistance developed against most of insecticides has made them ineffective. Attempts should always be made to find proper alternatives to control this insect. Methods: In the present study, B. germanica was collected from a hospital in Tehran. Insecticide susceptibility of the cockroaches to cypermethrin was determined using the WHO glass jar method and discriminating doses.Mortality was assessed at 14 h intervals after exposure to bait. Results: The results showed a resistance ratio of about 3.4 to cypermethrin and a complete susceptibility of the field strain to Hydramethylnon gel bait insecticide when they were compared with the susceptible strain. Therefore, there was no cross resistance pattern between these insecticides. This report is the first study on the susceptibility status of resistantB. germanica to Hydramethylnon bait in Iran. Conclusions:We suggest that the use of Hydramethylnon gel bait could be used as an effective control method especially when other insecticides fail to control the cockroach populations0.0.}, keywords = {Blattella gemanica,Resistance,Cypermethrin,Hydramethylnon}, url = {https://jkmu.kmu.ac.ir/article_83081.html}, eprint = {https://jkmu.kmu.ac.ir/article_83081_084e21645166ad087164f4a06f75eda3.pdf} } @article { author = {Danesh, Behnaz and Mohammadi, Abbas and Fallah, Hossein and Allahyari, Mostafa}, title = {The Effects of Simvastatin on Free Fatty Acids Profile in Fructose-fed Insulin Resistant Rats}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {405-413}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {Backgrounds: Type 2 diabetes mellitus is the most common metabolic disease and free fatty acids, as signaling molecules, can play a crucial role in the development of it. Different free fatty acids, through various cell membrane receptors, induce different effects on metabolic pathways and thereby affect insulin sensitivity. Simvastatin is a cholesterol decreasing drug prescribed for hypercholesterolemic patients. Due to the observing insulin-sensitizing effect of simvastatin in our previous study, we decided to evaluate its effects on free fatty acids profile as a probable mechanism for alteration of insulin sensitivity. Methods: Insulin resistance was developed in male Wistar rats by a high fructose diet for 6 weeks. After this period, animals were treated by intragastric injection of simvastatin for two weeks. Blood samples were collected in EDTA treated tubes and plasma was separated. After isolation of free fatty acids from other lipids fractions, free fatty acids profile were analyzed by gas chromatography system. Results: Simvastatin increased the concentrations of medium chains free fatty acids such as hexanoic acid, octanoic acid, decanoic acid, undecanoic acid, and dodecanoic acid. Simvastatin also increased plasma total free fatty acids concentration. Conclusion: The results of this study demonstrated that simvastatin by increasing the level of medium and long-chain free fatty acids can increase insulin secretion; on the other hand, simvastatin by increasing the level of total and saturated free fatty acids, could induce insulin resistance. More studies are necessary for the evaluation of the precise involved mechanisms in simvastatin effects on insulin function.}, keywords = {insulin resistance,Free fatty acids,Simvastatin,Gas Chromatography,Diabetes Mellitus}, url = {https://jkmu.kmu.ac.ir/article_83082.html}, eprint = {https://jkmu.kmu.ac.ir/article_83082_b78fcc43b6ee6f41cdebe674b5cdfb30.pdf} } @article { author = {Sofiabadi, Mohamad and Haghdoost-Yazdi, Hashem and Piri, Hossein and Esmaeili, Mohamad}, title = {B Vitamins Supplement Potentiates Antiparkinsonian Effect of Flunarizine: the Behavioral and Biochemical Evidences From 6-Hydroxydopamine Animal Model}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {414-427}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {Introduction: Prominent data indicate that flunarizine (flu), a calcium channel blocker, has neuroprotective effect. However, several authors have reported that the chronic use of flu can produce drug-induced Parkinsonism. Previously, we showed that B vitamins supplement (B com) has antiparkinsonian effect. In the present study, we evaluated the effect of pretreatment with flu and a combination of flu and B com on the 6-hydroxydopamine (6-OHDA) - induced Parkinsonism. Methods: 6-OHDA (4 μl, 4 μg/μl) was injected into right striatum by stereotaxic surgery. Different groups of rats received flu (5 or 10 mg/kg) or B com or a combination of them before the toxin to three weeks after that. The severity of Parkinsonism was assessed by conventional behavioral tests and also biochemical measurement of striatal dopamine level. Furthermore, malondialdehyde (MDA) concentration was measured in the serum and brain suspension. Results: Pretreatments with flu or B com significantly attenuated apomorphine- induced rotations and improved rotarod performance, but they had little effect on the 6- OHDA- induced swinging behavior. The pretreatments also reduced the decreasing effect of 6- OHDA on the striatal dopamine level. These antiparkinsonian effects were potentiated when animals were pretreated with a combination of flu and B com. In addition, B com alone or in combination with flu reduced MDA concentration especially in the brain tissue. On the other hand, flu increased MDA concentration in the serum. Conclusion: Our data show that co-administration of B com with flu potentiate largely the antiparkinsonian effect and may attenuate its adverse effects.}, keywords = {6-hydroxydopamine,Flunarizine,B vitamins,Striatal dopamine level,Malondialdehyde}, url = {https://jkmu.kmu.ac.ir/article_83083.html}, eprint = {https://jkmu.kmu.ac.ir/article_83083_ebaa63ecf02de31a780db16fb6946fb8.pdf} } @article { author = {Firouz, Farnaz and Izadi, Alireza and Fotovat, Farnoush and Ghorbani Gholiabad, Somaye and nikanjam, Saeed}, title = {The Effect of Different Solutions on Optical Behavior of Polished and Glazed Zirconia-Reinforced Lithium Silicate Ceramics}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {428-440}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {Background: This study sought to assess the effect of glazing and polishing surface treatments on the optical behavior of zirconia-reinforced lithium silicate ceramics thermocycled in different solutions. Methods:Computer-aided design/computer-aided manufacturing ceramics (VITA Suprinity) were cut into samples (n=104) measuring 1.5x14x10 mm according to the manufacturer’s instructions. Also, the samples were then randomly divided into four groups for immersion in artificial saliva, tea, orange juice and cola. A spectrophotometer was used to assess the color change and changes in L* coordinate against a white and a black background. The translucency parameter and contrast ratio were also calculated. The ∆E and L* values were analyzed using two-way ANOVA test. Student’s t-test was applied to analyze TP and CR. Results: The interaction effect of the type of solution and type of surface treatment on color change against a white (P=0.008) and black (p <0.001) background was statistically significant. Immersion of ceramic samples in orange juice and cola caused significant changes in TP and CR in the polishing and glazing groups (P < 0.05). Conclusion:Immersion in orange juice causes clinically perceivable color change in glazed ZLS ceramics. Immersion in cola decreases the translucency of both polished and glazed ZLS ceramics.}, keywords = {Dental ceramics,Zirconia reinforced ceramics,CAD/CAM ceramic,Optical properties,Translucency}, url = {https://jkmu.kmu.ac.ir/article_83084.html}, eprint = {https://jkmu.kmu.ac.ir/article_83084_a711efde336dbdb912dbf1070bd1980a.pdf} } @article { author = {Mansuri, Negin and abbaszadeh, hamid and Sharbatdaran, Majid and Saghravanian, Nasrollah and Gholinia, Hemmat}, title = {Myofibroblasts in calcifying odontogenic cyst and dentigerous cyst}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {441-445}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {Introduction:Calcifying odontogenic cyst (COC) is an odontogenic cyst that sometimes shows aggressive behavior, while dentigerous cyst (DC) always has a benign nonaggressive course. There are evidences that myofibroblasts are involved in invasion and their role in biologic behavior of odontogenic cysts has been less understood. So, the aim of the present study was to compare the role of myofibroblasts in COC and DC. Methods:In this cross-sectional study, paraffin-embedded tissue blocks of 20 COCs and 20 DCs were studied. Four-micron sections were prepared from tissue blocks and stained with α-SMA antibody using immunohistochemistry. Percentages of myofibroblasts were semi quantitatively classified into negative (<5% of cells were positive), weak (5-50% cells were positive) and strong (>50% of cells were positive). Percentage of myofibroblasts was compared between COC and DC groups using t-test and Chi-Square statistical tests. Results:Means of myofibroblasts percentages in COC and DC groups were respectively 35±33 and 32±30 that did not show significant difference between COC and DC groups (P=0.76). There was not any significant difference between COC and DC groups with respect to semi quantitative classification of percentage of myofibroblasts (P=0.62). Conclusion:Myofibroblasts probably do not play a significant role in different biologic behaviors of calcifying odontogenic and dentigerous cysts.}, keywords = {Calcifying odontogenic cyst,Dentigerous cyst,Myofibroblasts}, url = {https://jkmu.kmu.ac.ir/article_83085.html}, eprint = {https://jkmu.kmu.ac.ir/article_83085_eb1271d715988f5e088dbb7ab5137025.pdf} } @article { author = {Dadpour, Bita and Silakhori, Samaneh and Tajoddini, Shahrad and Jafari Shahroudi, Mahdieh and Najaf Najafi, Mona}, title = {Lead Poisoning: a 12-year Report from North East of Iran}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {446-455}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {Background: Lead poisoning is an issue of concern in developing countries and high levels of lead in blood could be resulted following occupational and environmental exposures. Several reports have raised concerns on the increasing prevalence of lead intoxication among opioid addicts. This study investigated the clinical manifestations and demographic data of subjects who were diagnosed with lead poisoning and also possible correlations of job type and opium abuse with lead poisoning. Methods: This cross-sectional study was carried out on 82 patients admitted to Imam Reza Hospital, Mashhad, from 2004 to 2016 with the diagnosis of lead poisoning. Demographic information, clinical signs and symptoms, as well as, hematological and biochemical profiles and their possible correlations were investigated. Opium-addiction status was judged based on patient self-report. Results: In this study, 82 patients were admitted to Toxicology Department. Patients’ age ranged between 19 and 81 years old (mean ±SD: 42.2±14.90) and 78 patients were male (95.1%). With regard to the occupational exposure, 29 individuals (35.4%) had occupational risk factors for lead poisoning; however, no significant relation was found between the type of occupation and blood lead level (p=0.95). Moreover, 43 individuals (52.4%) were opioid-addicted but opioid addiction was not significantly correlated with blood lead level (p=0.91). Among all, 70 individuals (85.4%) were anemic and with increasing blood lead level, the levels of Hb (p= 0.011, r= -0.279) and Hct (p=0.003, r= -0.332) showed significant decrease. Conclusion: Though blood lead level was not significantly correlated with opioid addiction, but most of patients were opioid addicts. Apparently, public awareness and health-care-providers’ knowledge should be improved about the potential hazardous effects of using adulterated opium.}, keywords = {Lead Poisoning,Heavy metal poisoning,Lead Intoxication,Addiction}, url = {https://jkmu.kmu.ac.ir/article_83086.html}, eprint = {https://jkmu.kmu.ac.ir/article_83086_edaa958298a04304d470870284fcd5fe.pdf} } @article { author = {Ezzatabadipour, Massood and Nematollahi-mahani, Seyed Noureddin and Hosseini, Ahmad and babaee, abdolreza}, title = {Pregnancy in a Breastfeeding Mouse without Mating}, journal = {Journal of Kerman University of Medical Sciences}, volume = {25}, number = {5}, pages = {456-461}, year = {2018}, publisher = {Kerman University of Medical Sciences}, issn = {1023-9510}, eissn = {2008-2843}, doi = {}, abstract = {In this report, a rare case of pregnancy in a mouse without mating and during breastfeeding is presented. Out-bred NMRI mice were prepared for mating. Two female mice without superovulation were caged with one male. The next morning, vaginal plaque was not detected; therefore, females were kept in the cage with the same male for 4 more days. One of the females delivered 4 offspring: one female and three males. This female conceived again without mating, 21 days after the first delivery and during the breastfeeding period and this time delivered 16 offspring: 13 males and 3 females. The more likely explanation for this rare, second parturition is embryonic diapause.}, keywords = {Pregnancy,Breastfeeding,Embryonic diapause,Parthenogenesis,Mouse}, url = {https://jkmu.kmu.ac.ir/article_83087.html}, eprint = {https://jkmu.kmu.ac.ir/article_83087_516aa809579eea0b91225b1217576705.pdf} }