%0 Journal Article %T Evaluation of the Cytotoxicity, Antibacterial, Antioxidant, and Anti-inflammatory Effects of Different Extracts of Punica granatum var. pleniflora %J Journal of Kerman University of Medical Sciences %I Kerman University of Medical Sciences %Z 1023-9510 %A Mehrabani, Mehrnaz %A Raeiszadeh, Mahboobeh %A Najafipour, Hamid %A Esmaeli Tarzi, Mojde %A Amirkhosravi, Arian %A Poustforoosh, Alireza %A Mohammadi, Mohammad Ali %A Naghdi, Sara %A Mehrabani, Mitra %D 2020 %\ 09/01/2020 %V 27 %N 5 %P 414-425 %! Evaluation of the Cytotoxicity, Antibacterial, Antioxidant, and Anti-inflammatory Effects of Different Extracts of Punica granatum var. pleniflora %K Punica granatum var. pleniflora Anti %K inflammation Antioxidant Antibacterial Cytotoxicity Murine macrophage J774A.1 %R 10.22062/jkmu.2020.91474 %X Background:Punica granatum var. pleniflora (PGP) has been used for thousands of years as an effective agent to treat various types of diseases. However, there are a few new evidences addressing its therapeutic effects and related mechanisms. Therefore, the aim of this study was to investigate the cytotoxic, antioxidant, antibacterial, and anti-inflammatory effects of ethanolic (ET), dichloromethane (DM), and ethyl acetate (EA) extracts of PGP. Methods: ET, DM, and EA extracts of PGP were first prepared using maceration method. Total phenolic content (TPC) of PGP was then assessed by the Folin-Ciocalteu assay, and its antioxidant capacity was determined by DPPH and FRAP methods. Furthermore, in-vitro antibacterial activity of the PGP extracts was performed. The effect of PGP on the viability of J774A.1, HUVECs, HT29, and MCF-7 cell lines was evaluated by the MTT assay. The anti-inflammatory effect of PGP was assessed in the lipopolysaccharide (LPS)-stimulated J774A.1 cell line using qRT-PCR method. Results: EA extract contained the highest phenolic content (383.3 ± 9.1 mg gallic acid/g extract) and showed the highest antioxidant activity (IC50 = 36.5 ± 2.3 µg/mL). PGP at concentration of 15 µg/mL significantly decreased the expression of COX-2 (ET) and iNOS (ET and EA) in J774A.1 cell. Also, EA showed the highest antibacterial activity. Furthermore, the PGP extracts decreased the viability of all tested cell lines in a concentration-dependent manner. As indicated by IC50, EA demonstrated the lowest IC50 for all tested cell lines. Conclusion: According to the results, antioxidant, anti-inflammatory, antibacterial, and cytotoxic effects of PGP might be driven by its phenolic compounds highly presented in the EA extract. %U https://jkmu.kmu.ac.ir/article_91474_96cdafb062c30f332bc1b2f421cc4312.pdf