ORIGINAL_ARTICLE
Epidermal Growth Factor Receptor Expression in Oral Squamous Cell Carcinoma by Immunohistochemical Technique and its Correlation with Clinicopathological Features
Background:Oral squamous cell carcinoma (OSCC) is the most common malignancy of the oral cavity. Despite some improvements in treatment, the survival rate is still very low, mainly due to the possible development of secondary malignancy or metastasis. Clinical and pathological features as well as molecular biomarkers might predict the recurrence. In recent years, many studies have been carried out on molecular biomarkers that can predict the prognosis of OSCC. One of these markers is the epidermal growth factor receptor (EGFR), which has led to different results. The aim of this study was to determine EGFR level in OSCC and to analyze its correlation with clinicopathological features. Methods: A total of 62 paraffin-embedded samples from OSCC patients treated in the oncology department of the Omid Hospital in the city of Mashhad, Iran were selected and EGFR staining was performed. The clinical and histopathological data were extracted from the medical records. Results: EGFR expression was positive in 98.4% of the cases. There was a significant difference between EGFR expression in the tumor and control cases in terms of cellularity and intensity (p˂0.001 and p=0.004, respectively). No statistically significant correlation was observed between EGFR and clinicopathological parameters. There was also no significant relationship between the cellularity and intensity expression of EGFR and patient survival (p=0.92 and p=0.42, respectively). Conclusion: In view of the high EGFR expression in squamous cell carcinoma, further studies on the role of EGFR in cell processes such as proliferation, angiogenesis and differentiation of the tumor are recommended.
https://jkmu.kmu.ac.ir/article_91015_71ba1a85beba1861481e5f4cfcd15da1.pdf
2020-07-01
283
293
10.22062/jkmu.2020.91015
Oral carcinoma
Squamous cell
Epidermal Growth Factor
survival rate
immunohistochemistry
Zohreh
Dalirsani
dalirsaniz@mums.ac.ir
1
Associate Professor of Oral and Maxillofacial Medicine , Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Bahram
Memar
memarb@mums.ac.ir
2
Associate Professor, Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Atessa
Pakfetrat
pakfetrata@mums.ac.ir
3
Professor of Oral and Maxillofacial Medicine, Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
Nooshin
Mohtasham
mohtashamn@mums.ac.ir
4
Professor of Oral and Maxillofacial pathology, Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Kazem
Anvari
anvarik@mums.ac.ir
5
Associate Professor, Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Sara
kaveh
sara.kaveh@semums.ac.ir
6
Specialist of Cosmetic and Restorative Dentistry, Tehran, Iran
AUTHOR
Jonsson EL, Nylander K, Hallen L, Laurell G. Effect of radiotherapy on expression of hyaluronan and EGFR and presence of mast cells in squamous cell carcinoma of the head and neck. Oncol Lett 2012; 4(6):1177-82.
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Zuo JH, Zhu W, Li MY, Li XH, Yi H, Zeng GQ, et al. Activation of EGFR promotes squamous carcinoma SCC10A cell migration and invasion via inducing EMT-like phenotype change and MMP-9-mediated degradation of E-cadherin. J Cell Biochem 2011; 112(9):2508-17.
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Eriksen JG, Steiniche T, Askaa J, Alsner J, Overgaard J. The prognostic value of epidermal growth factor receptor is related to tumor differentiation and the overall treatment time of radiotherapy in squamous cell carcinomas of the head and neck. Int J Radiat Oncol Biol Phys 2004; 58(2):561-6.
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Chuang CY, Chen MK, Hsieh MJ, Yeh CM, Lin CW, Yang WE, et al. High level of plasma EGFL6 is associated with clinicopathological characteristics in patients with oral squamous cell carcinoma. Int J Med Sci 2017; 14(5):419-24.
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Mahipa A, Mcdonald M, Witkiewicz A, Carr BI. Cell membrane and cytoplasmic epidermal growth factor receptor expression in pancreatic ductal adenocarcinoma. Med Oncol 2011; 29(1):134-9.
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Kong Q, Majeska RJ, Vazquez M. Migration of connective tissue-derived cells is mediated by ultra-low concentration gradient fields of EGF. Exp Cell Res 2011; 317(11):1491-502.
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Chung CH, Ely K, McGavran L, Varella-Garcia M, Parker J, Parker N, et al. Increased epidermal growth factor receptor gene copy number is associated with poor prognosis in head and neck squamous cell carcinomas. J Clin Oncol 2006; 24(25):4170-6.
36
Licitra L, Mesia R, Rivera F, Remenar E, Hitt R, Erfan J, et al. Evaluation of EGFR gene copy number as a predictive biomarker for the efficacy of cetuximab in combination with chemotherapy in the first-line treatment of recurrent and/or metastatic squamous cell carcinoma of the head and neck: EXTREME study. Ann Oncol 2011; 22(5):1078-87.
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Gao J, Ulekleiv CH, Halstensen TS. Epidermal growth factor (EGF) receptor-ligand based molecular staging predicts prognosis in head and neck squamous cell carcinoma partly due to deregulated EGF- induced amphiregulin expression. J Exp Clin Cancer Res 2016; 35(1):151.
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Smid EJ, Stoter TR, Bloemena E, Lafleur MV, Leemans CR, Van Der Waal I, et al. The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy. Int J Radiat Oncol Biol Phys 2006; 65(5):1323-9.
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45
ORIGINAL_ARTICLE
The Impact of Cardiac Rehabilitation on Pulmonary Artery Systolic Pressure and Left Ventricular End-Diastolic Pressure in Patients after Coronary Artery Bypass Graft Surgery
Background:Cardiac rehabilitation program (CRP) is a useful method of modifying cardiovascular risk factors, improving life expectancy and quality of life in patients with ischemic heart disease (IHD). The present study was conducted to evaluate the effects of cardiac rehabilitation on the pulmonary artery systolic pressure (PASP) and left ventricular end-diastolic pressure (LVEDP). Methods: This Quasi-experimental study with pretest-posttest design was conducted on 80 patients with IHD who had participated in CRP after undergoing coronary artery bypass graft (CABG) surgery. Echocardiography was performed before the beginning of CRP (the 1st session) and at the end of the rehabilitation sessions, and ventricular function indices (ejection fraction), PASP (using the tricuspid regurgitation velocity), and LVEDP (using Nagueh formula: 1.24×E/e´+1.9) were measured. Results: Ejection fraction (EF) was changed from 49.3 ± 7.8 before rehabilitation to 50.7 ± 7.4 after rehabilitation, which was a statistically significant difference (P=0.003). The pulmonary artery systolic pressure altered from 30.3 ± 8.4 before rehabilitation to 27.3 ± 6.6 after rehabilitation. The left ventricular end-diastolic pressure (LVEDP) changed from 10.5 ± 3.7 before rehabilitation to 9.1 ± 2.9 after rehabilitation, which was a statistically significant difference (P= 0.000). Conclusion: According to the results, LVEDP and PASP in patients with IHD who underwent CABGs decreased after cardiac rehabilitation.
https://jkmu.kmu.ac.ir/article_91016_c0f21618e9c06acff3e3b2176a26c811.pdf
2020-07-01
294
303
10.22062/jkmu.2020.91016
Cardiac Rehabilitation Coronary Artery Bypass Graft Left Ventricular End
diastolic Pressure Pulmonary Artery Pressure Blood Pressure
Mansoor
Moazenzadeh
m_moazenzadeh@kmu.ac.ir
1
Associate Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, Iran
AUTHOR
Khadijeh
Mohammadi
2
Assistant Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, Iran
AUTHOR
Afshin
Sarafi Nejad
a.sarafi@gmail.com
3
Clinical Informatics Research and Development Lab, Shafa Clinical Research Unit, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Fatemeh
Karimi Afshar
f.karimi.afshar@gmail.com
4
Assistant Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, Iran
AUTHOR
Hamidreza
Rashidinejad
h.rashidinejad@gmail.com
5
Associate Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, Iran
LEAD_AUTHOR
Balady GJ, Williams MA, Ades PA, Bittner V, Comoss P, Foody JM, et al. Core components of cardiac rehabilitation/secondary prevention programs: 2007 update a scientific statement from the American Heart Association exercise, cardiac rehabilitation, and prevention committee, the council on clinical cardiology; the councils on cardiovascular nursing, epidemiology and prevention, and nutrition, physical activity, and metabolism; and the American Association of Cardiovascular and Pulmonary Rehabilitation. Circulation 2007; 115(20):2675-82.
1
Woods SL, Sivarajan Froelicher ES, Motzer SA, Bridges EJ. Cardiac Nursing. 6th ed. China: LWW; 2009.
2
O'Sullivan SB, Schmitz TJ, Fulk G. Physical Rehabilitation. 6th ed. Philadelphia: F. A. Davis; 2013.
3
Yu CM, Lau CP, Chau J, McGhee S, Kong SL, Cheung BM, et al. A short course of cardiac rehabilitation program is highly cost effective in improving long-term quality of life in patients with recent myocardial infarction or percutaneous coronary intervention. Arch Phys Med Rehabil 2004; 85(12):1915-22.
4
Kulcu DG, Kurtais Y, Tur BS, Gulec S, Seckin B. The effect of cardiac rehabilitation on quality of life, anxiety and depression in patients with congestive heart failure: a randomized controlled trial, short-term results. Eura Medicophys 2007; 43(4):489-97.
5
Jelinek MV, Thompson DR, Ski C, Bunker S, Vale MJ. 40 years of cardiac rehabilitation and secondary prevention in post-cardiac ischaemic patients. Are we still in the wilderness? Int J Cardiol 2015; 179:153-9.
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10
Yamamoto T, Okada O, Tanabe N, Yasuda J, Satou K, Saitou M, et al. Relation of pulmonary vascular response to pressure-flow relationship during incremental exercise in patients with chronic obstructive pulmonary disease (COPD). Nihon Kyobu Shikkan Gakkai Zasshi 1994; 32(3):225-32.
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12
Wuthiwaropas P, Bellavia D, Omer M, Squires RW, Scott CG, Pellikka PA. Impact of cardiac rehabilitation exercise program on left ventricular diastolic function in coronary artery disease: a pilot study. Int J Cardiovasc Imaging 2013; 29(4):777-85.
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Yu CM, Li LS, Lam MF, Siu DC, Miu RK, Lau CP. Effect of a cardiac rehabilitation program on left ventricular diastolic function and its relationship to exercise capacity in patients with coronary heart disease: experience from a randomized, controlled study. Am Heart J 2004; 147(5):e24.
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Basati F, Kargarfard M, Sadeghi M, Golabchi A, Rozbahani R. Effects of a cardiac rehabilitation program on left ventricular systolic function and mass in patient after myocardial infarction. Journal of Isfahan Medical School 2012; 30(187):561-71. [In Persian].
15
Taylor RS, Brown A, Ebrahim S, Jolliffe J, Noorani H, Rees K, et al. Exercise-based rehabilitation for patients with coronary heart disease: systematic review and meta-analysis of randomized controlled trials. Am J Med 2004; 116(10):682-92.
16
Meurs M, Burger H, van Riezen J, Slaets JP, Rosmalen JG, van Melle JP, et al. The association between cardiac rehabilitation and mortality risk for myocardial infarction patients with and without depressive symptoms. Journal of Affective Disorders 2015; 188:278-83.
17
Rutledge T, Redwine LS, Linke SE, Mills PJ. A meta-analysis of mental health treatments and cardiac rehabilitation for improving clinical outcomes and depression among patients with coronary heart disease. Psychosom Med 2013; 75(4):335-49.
18
Giannuzzi P, Temporelli PL, Corra U, Gattone M, Giordano A, Tavazzi L. Attenuation of unfavorable remodeling by exercise training in postinfarction patients with left ventricular dysfunction: results of the Exercise in Left Ventricular Dysfunction (ELVD) trial. Circulation 1997; 96(6):1790-7.
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Kargarfard M, Rouzbehani R, Basati F. Effects of exercise rehabilitation on blood pressure of patients after myocardial infarction. Int J Prev Med 2010; 1(2):124-30.
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21
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22
Abtahi F, Tahamtan M, Homayouni K, Moaref A, Zamirian M. The assessment of cardiac rehabikitation on echocardiographic Parameters of left ventricular systolic functionin patient treated by primary percutaneous coronary intervention due to acute ST segmentelevation myocardial infarction :a randomized clinical trial. Int Cardiovasc Res j 2017; 11(4):130-6.
23
Temporelli PL, Giannuzzi P, Nicolosi GL, Latini R, Franzosi MG, Gentile F, et al. Doppler-derived mitral deceleration time as a strong prognostic marker of left ventricular remodeling and survival after acute myocardial infarction: results of the GISSI-3 echo substudy. J Am Coll Cardiol 2004; 43(9):1646-53.
24
Hillis GS, Møller JE, Pellikka PA, Gersh BJ, Wright RS, Ommen SR, et al. Noninvasive estimation of left ventricular filling pressure by E/e′ is a powerful predictor of survival after acute myocardial infarction. J Am Coll Cardiol 2004; 43(3):360-7.
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Whalley GA, Gamble GD, Doughty RN. Restrictive diastolic filling predicts death after acute myocardial infarction: systematic review and meta-analysis of prospective studies. Heart 2006; 92(11):1588-94.
26
Tongyoo S, Jakrapanichakul D, Chaowalit N. Estimation of left ventricular end-diastolic pressure by tissue doppler imaging in patients with coronary artery disease. Thai Heart J 2006; 19(3):105-13.
27
Nagueh SF, Appleton CP, Gillebert TC, Marino PN, Oh JK, Smiseth OA, et al. Recommendations for the evaluation of left ventricular diastolic function by echocardiography. J Am Soc Echocardiogr 2009; 22(2):107-33.
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Nagueh SF, Middleton KJ, Kopelen HA, Zoghbi WA, Quinones MA. Doppler tissue imaging: a noninvasive technique for evaluation of left ventricular relaxation and estimation of filling pressures. J Am Coll Cardiol 1997; 30(6):1527-33.
29
Ozer N, Kepez A, Kaya B, Kilic H, Deniz A, Arslan U, et al. Determination of left ventricular filling pressure by new echocardiographic methods in patients with coronary artery disease. Int J Cardiovasc Imaging 2008; 24(2):141-7.
30
Giallauria F, Lucci R, De Lorenzo A, D’Agostino M, Del Forno D, Vigorito C. Favourable effects of exercise training on N-terminal pro-brain natriuretic peptide plasma levels in elderly patients after acute myocardial infarction. Age Ageing 2006; 35(6):601-7.
31
Xu X, Wan W, Powers AS, Li J, Ji LL, Lao S, et al. Effects of exercise training on cardiac function and myocardial remodeling in post myocardial infarction rats. J Mol Cell Cardiol 2008; 44(1):114-22.
32
Malfatto G, Branzi G, Osculati G, Valli P, Cuoccio P, Ciambellotti F, et al. Improvement in left ventricular diastolic stiffness induced by physical training in patients with dilated cardiomyopathy. J Card Fail 2009; 15(4):327-33.
33
Golabchi A, Basati F, Kargarfard M, Sadeghi M. Can cardiac rehabilitation programs improve functional capacity and left ventricular diastolic function in patients with mechanical reperfusion after ST elevation myocardial infarction?: A double-blind clinical trial. ARYA Atheroscler 2012; 8(3):125-29.
34
Guazzi M, Galiè N. Pulmonary hypertension in left heart disease. European Respiratory Review 2012; 21(126):338-46.
35
Sahni S, Capozzi B, Iftikhar A, Sgouras V, Ojrzanowski M, Talwar A. Pulmonary rehabilitation and exercise in pulmonary arterial hypertension: an underutilized intervention. J Exerc Rehabil 2015; 11(2):74-9.
36
Weinstein AA, Chin LM, Keyser RE, Kennedy M, Nathan SD, Woolstenhulme JG, et al. Effect of aerobic exercise training on fatigue and physical activity in patients with pulmonary arterial hypertension. Respir Med 2013; 107(5):778-84.
37
Dubach P, Myers J, Dziekan G, Goebbels U, Reinhart W, Muller P, et al. Effect of high intensity exercise training on central hemodynamic responses to exercise in men with reduced left ventricular function. J Am Coll Cardiol 1997; 29(7):1591-8.
38
Newman JH, Robbins IM. Exercise training in pulmonary hypertension implications for the evaluation of drug trials. Circulation 2006; 114(14):1448-9.
39
Mereles D, Ehlken N, Kreuscher S, Ghofrani S, Hoeper MM, Halank M, et al. Exercise and respiratory training improve exercise capacity and quality of life in patients with severe chronic pulmonary hypertension. Circulation 2006; 114(14):1482-9.
40
Grunig E, Maier F, Ehlken N, Fischer C, Lichtblau M, Blank N, et al. Exercise training in pulmonary arterial hypertension associated with connective tissue diseases. Arthritis Res Ther 2012; 14(3):R148.
41
Maeda S, Tanabe T, Miyauchi T, Otsuki T, Sugawara J, Iemitsu M, et al. Aerobic exercise training reduces plasma endothelin-1 concentration in older women. J Appl Physiol (1985) 2003; 95(1):336-41.
42
ORIGINAL_ARTICLE
The Effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) Inhibition on Metabolic Flexibility during Endurance Training in Skeletal Muscles of Streptozotocin-induced Diabetic Rats
Background:Metabolic flexibility is the capacity of a system to adjust fuel (primarily glucose and fatty acids) oxidation based on nutrient availability. Pyruvate Dehydrogenase Kinase 4 (PDK4) is one of the main enzymes that play a critical role in metabolic flexibility. In current study, we examined PDK4 inhibition along with exercise training (ET) on the gene expression of Estrogen related-receptor alpha (ERRα), medium-chain acyl-CoA dehydrogenase (MCAD), carnitine palmitoyl transferase-1b (CPT-1b), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), PDK4 and citrate synthase (CS) in skeletal muscle.Method:Sixty-four male Wistar rats (8 week-old) were randomly divided into 8 groups (n=8); 1- untreated control, 2- STZ-induced diabetic, 3- PDK4 inhibition, 4- endurance training (ET), 5- diabetic + PDK4 inhibition, 6- diabetic + ET, 7- PDK4 inhibition + ET, and 8- diabetic +ET + PDK4 inhibition. ERRα, MCAD, CPT-1b, PGC-1α, PDK4 and CS genes expressions were measured by Real-Time PCR and quantified by 2-ΔΔCt method.Results:ERRα, MCAD, CPT-1b, PGC-1α, PDK4, and CS expressions were significantly higher in non-diabetic+ Endurance Training group compared to the control group. The expressions of CPT-1b, MCAD and CS genes were significantly lower in the non-diabetic+ endurance training/PDK4 inhibition compared to the non-diabetic+ endurance training group, and the expressions of ERRα, CPT-1b and MCAD were significantly lower in the diabetic + PDK4 inhibition group compared to the diabetic group.Conclusion:In sum, PDK4 inhibition has negative effects on lipid metabolism in healthy rats, but in animals with diabetes, PDK4 inhibition can be used for improving lipid metabolism (over-expression of CS and PGC-1α).
https://jkmu.kmu.ac.ir/article_91017_021aed81532676834f8ec4d03e11a763.pdf
2020-07-01
304
317
10.22062/jkmu.2020.91017
Pyruvate Dehydrogenase Kinase 4 Endurance Training Metabolic Flexibility Estrogen
Related Receptor
Hamid
Marefati
1
Associate Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Yaser
Masoumi-Ardakani
2
Ph.D. Candidate, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Saeed
Shakerian
soleimaniasma47@yahoo.com
3
Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Abdolhamid
Habibi
s.amini@sport.uk.ac.ir
4
Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Soheil
Aminizadeh
soheilaminizadeh@gmail.com
5
Assistant Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
LEAD_AUTHOR
Beydolah
Shahouzehi
bshahouzehi@gmail.com
6
Assistant Professor, Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences & Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Smekal G, von Duvillard SP, Pokan R, Tschan H, Baron R, Hofmann P, et al. Effect of endurance training on muscle fat metabolism during prolonged exercise: agreements and disagreements. Nutrition 2003; 19(10):891-900.
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Wajner M, Amaral AU. Mitochondrial dysfunction in fatty acid oxidation disorders: insights from human and animal studies. Biosci Rep 2015; 36(1):e00281.
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Suwa M, Nakano H, Radak Z, Kumagai S. Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor gamma coactivator-1alpha protein expressions in rat skeletal muscle. Metabolism 2008; 57(7):986-98.
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Tripathi M, Yen PM, Singh BK. Estrogen-related receptor alpha: an under-appreciated potential target for the treatment of metabolic diseases. Int J Mol Sci 2020; 21(5):1645.
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16
Wang T, McDonald C, Petrenko NB, Leblanc M, Wang T, Giguere V, et al. Estrogen-related receptor alpha (ERRalpha) and ERRgamma are essential coordinators of cardiac metabolism and function. Mol Cell Biol 2015; 35(7):1281-98.
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Huang B, Wu P, Popov KM, Harris RA. Starvation and diabetes reduce the amount of pyruvate dehydrogenase phosphatase in rat heart and kidney. Diabetes 2003; 52(6):1371-6.
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23
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Mostafavinia A, Amini A, Ghorishi SK, Pouriran R, Bayat M. The effects of dosage and the routes of administrations of streptozotocin and alloxan on induction rate of type1 diabetes mellitus and mortality rate in rats. Lab Anim Res 2016; 32(3):160-5.
26
Mansouri M, Nikooie R, Keshtkar A, Larijani B, Omidfar K. Effect of endurance training on retinol-binding protein 4 gene expression and its protein level in adipose tissue and the liver in diabetic rats induced by a high-fat diet and streptozotocin. J Diabetes Investig 2014; 5(5):484-91.
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Ping Z, Zhang LF, Cui YJ, Chang YM, Jiang CW, Meng ZZ, et al. The Protective Effects of Salidroside from Exhaustive Exercise-Induced Heart Injury by Enhancing the PGC-1 alpha -NRF1/NRF2 Pathway and Mitochondrial Respiratory Function in Rats. Oxidative Medicine and Cellular Longevity 2015; 2015:876825.
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Hu JZ, Long H, Wu TD, Zhou Y, Lu HB. The effect of estrogen-related receptor alpha on the regulation of angiogenesis after spinal cord injury. Neuroscience 2015; 290:570-80.
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Deblois G, Giguere V. Functional and physiological genomics of estrogen-related receptors (ERRs) in health and disease. Biochim Biophys Acta 2011; 1812(8):1032-40.
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40
ORIGINAL_ARTICLE
A Novel in vitro Co-culture Systems on Differentiation of Embryonic Stem Cells into Oocyte-like Cells in an in vivo Manner
Background:Differentiation of Embryonic Stem Cells into Oocyte-like cells in vitro is challenging. Successful derivation of oocyte from stem cells can provide an alternative source for curing ovogenesis problems. The current study aims to demonstrate a new protocol with two different types of media for differentiating embryonic stem cells (ESCs) into oocyte-like cells (OLCs). Methods: After culturing mouse ESCs, embryoid bodies (EBs) were generated from ESCs by hanging drop (HD) method. To final differentiation of oocyte-like cells (OLCs), the EBs were cultured in two different types of media for 12 days (first 7 days EBs were cultured in in vitro maturation diluted in Granulose Cell- Conditioned Medium and Follicular Fluid [1:1:1] followed by 5 days of culture in in vitro maturation diluted in uterine condition medium [1:1] ). Results:According tothe MTT test, the viability rate increased in the experimental group compared to the control EBs cultured alone. Expression of Oct4, as a pluripotency marker, decreased during the differentiation process of EBs in the experimental group. Co-culturing of EBs with our mentioned protocol increased germ cell markers (Stella and Mvh) and increased Oocyte-specific markers (ZP1, Figα and GDF9). Conclusion:Our study introduces a promising in vitro protocol for achieving successful oogenesis through creating interactions of EBs with granulosa cells and uterine condition medium.
https://jkmu.kmu.ac.ir/article_91018_f44b9d540fea885c20ab85d716ee7f6c.pdf
2020-07-01
318
328
10.22062/jkmu.2020.91018
Embryonic Stem Cells
Conditioned Medium
Embryoid Bodies
in vitro maturation
Ali
Delbari
ali_delbari@yahoo.com
1
Assistant Professor, Cellular and Molecular Research Center & Department of Anatomical Sciences, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
AUTHOR
Maryam
Nazm Bojnordi
2
Assistant Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Sina
Mojaverrostami
3
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
Hatef
Ghasemi Hamidabadi
hatefdr@gmail.com
4
Associate Professor, Immunogenetic Research Center & Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
LEAD_AUTHOR
Zahra
Bagheri‑Hosseinabadi
5
Assistant Professor, Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran 6- Associate Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University
AUTHOR
Nourollah
Rezaei
nourrezaei@gmail.com
6
Associate Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Patel H, Bhartiya D. Testicular stem cells express follicle-stimulating hormone receptors and are directly modulated by FSH. Reprod Sci 2016; 23(11):1493-508.
1
Moussaieff A, Rouleau M, Kitsberg D, Cohen M, Levy G, Barasch D, et al. Glycolysis-mediated changes in acetyl-CoA and histone acetylation control the early differentiation of embryonic stem cells. Cell Metab 2015; 21(3):392-402.
2
Bhartiya D. Ovarian stem cells are always accompanied by very small embryonic-like stem cells in adult mammalian ovary. J Ovarian Res 2015; 8:70.
3
Bhartiya D, Shaikh A, Anand S, Patel H, Kapoor S, Sriraman K, et al. Endogenous, very small embryonic-like stem cells: critical review, therapeutic potential and a look ahead. Hum Reprod Update 2016; 23(1):41-76.
4
Khosravi-Farsani S, Amidi F, Roudkenar MH, Sobhani A. Isolation and enrichment of mouse female germ line stem cells. Cell J 2015; 16(4):406-15.
5
Kurkure P, Prasad M, Dhamankar V, Bakshi G. Very small embryonic-like stem cells (VSELs) detected in azoospermic testicular biopsies of adult survivors of childhood cancer. Reprod Biol Endocrinol 2015; 13:122.
6
Edessy M, Hosni HN, Shady Y, Waf Y, Bakr S, Kamel M. Autologous stem cells therapy, the first baby of idiopathic premature ovarian failure. Acta Medica International 2016; 3(1):19-23.
7
Makoolati Z, Movahedin M, Forouzandeh-Moghadam M. In vitro germ cell differentiation from embryonic stem cells of mice: induction control by BMP4 signalling. Biosci Rep 2016; 36(6): e00407.
8
Parvari S, Yazdekhasti H, Rajabi Z, Gerayeli Malek V, Rastegar T, Abbasi M. Differentiation of mouse ovarian stem cells toward oocyte-like structure by coculture with granulosa cells. Cell Reprogram 2016; 18(6):419-28.
9
Evron A, Blumenfeld Z. Ovarian stem cells–-the pros and cons. Clin Med Insights Reprod Health 2013; 7:43-7.
10
Hamidabadi HG, Pasbakhsh P, Amidi F, Soleimani M, Forouzandeh M, Sobhani A. Functional concentrations of BMP4 on differentiation of mouse embryonic stem cells to primordial germ cells. Int J Fertil Steril 2011; 5(2):104-9.
11
Qing T, Shi Y, Qin H, Ye X, Wei W, Liu H, et al. Induction of oocyte‐like cells from mouse embryonic stem cells by co‐culture with ovarian granulosa cells. Differentiation 2007; 75(10):902-11.
12
Acharya A, Brungs S, Henry M, Rotshteyn T, Singh Yaduvanshi N, Wegener L, et al. Modulation of differentiation processes in murine embryonic stem cells exposed to parabolic flight-induced acute hypergravity and microgravity. Stem Cells Dev 2018; 27(12):838-47.
13
Behringer R, Gertsenstein M, Nagy KV, Nagy A. Differentiating mouse embryonic stem cells into embryoid bodies by hanging-drop cultures. Cold Spring Harb Protoc 2016; 2016(12).
14
Li S, Wang M, Chen Y, Wang W, Wu J, Yu C, et al. Role of the hedgehog signaling pathway in regulating the behavior of germline stem cells. Stem Cells Int 2017; 2017:5714608.
15
Lai D, Wang F, Dong Z, Zhang Q. Skin-derived mesenchymal stem cells help restore function to ovaries in a premature ovarian failure mouse model. PLoS One 2014; 9(5):e98749.
16
Ge W, Ma HG, Cheng SF, Sun YC, Sun LL, Sun XF, et al. Differentiation of early germ cells from human skin-derived stem cells without exogenous gene integration. Scientific Reports 2015; 5:13822.
17
Tan H, Wang JJ, Cheng SF, Ge W, Sun YC, Sun XF, et al. Retinoic acid promotes the proliferation of primordial germ cell–like cells differentiated from mouse skin-derived stem cells in vitro. Theriogenology 2016; 85(3):408-18.
18
Ge W, Cheng SF, Dyce PW, De Felici M, Shen W. Skin-derived stem cells as a source of primordial germ cell-and oocyte-like cells. Cell Death Dis 2016; 7(11):e2471.
19
Yu X, Wang N, Qiang R, Wan Q, Qin M, Chen S, et al. Human amniotic fluid stem cells possess the potential to differentiate into primordial follicle oocytes in vitro. Biol Reprod 2014; 90(4):73.
20
Ghasemzadeh-Hasankolaei M, Batavani R, Eslaminejad MB, Sayahpour F. Transplantation of autologous bone marrow mesenchymal stem cells into the testes of infertile male rats and new germ cell formation. Int J Stem Cells 2016; 9(2):250-63.
21
Bhartiya D, Anand S, Patel H, Parte S. Making gametes from alternate sources of stem cells: past, present and future. Reprod Biol Endocrinol 2017; 15:89.
22
Brankin V, Mitchell MR, Webb B, Hunter MG. Paracrine effects of oocyte secreted factors and stem cell factor on porcine granulosa and theca cells in vitro. Reprod Biol Endocrinol 2003; 1(1):55.
23
Ge W, Chen C, De Felici M, Shen W. In vitro differentiation of germ cells from stem cells: a comparison between primordial germ cells and in vitro derived primordial germ cell-like cells. Cell Death Dis 2015; 6(10):e1906.
24
Yan G, Fan Y, Li P, Zhang Y, Wang F. Ectopic expression of DAZL gene in goat bone marrow‐derived mesenchymal stem cells enhances the trans‐differentiation to putative germ cells compared to the exogenous treatment of retinoic acid or bone morphogenetic protein 4 signalling molecules. Cell Biol Int 2015; 39(1):74-83.
25
Shah SM, Saini N, Singh MK, Manik R, Singla SK, Palta P, et al. Testicular cell–conditioned medium supports embryonic stem cell differentiation toward germ lineage and to spermatocyte-and oocyte-like cells. Theriogenology 2016; 86(3):715-29.
26
Rashidi B, Soleimani Rad JI, Roshangar L, Alizadeh Miran R. Evaluation of pinopodes expression on the mouse endometrium immediately before implantation by treatment with HMG/HCG and sildenafil citrate administration. Iran J Basic Med Sci 2012; 15(5):1091-6.
27
Heydari L, Noori Mugahi SM, Fazelipour S, Koruji M, Alizadeh R, Abbasi N, et al. Effects of ovarian varicose vein on mitochondrial structure, malondialdehyde and prooxidants: antioxidants balance in rat ovaries. International Journal of Morphology 2015; 33(3):930-5.
28
Mohammadi S, Gholamin M, Mansouri A, Mahmoodian RS, Babazadeh B, Kebriaei SM, et al. Effect of cadmium and nickel on expression of CatSper 1 and 2 genes in mice. Toxin Reviews 2018; 37(3):216-22.
29
Vértesy Á, Arindrarto W, Roost MS, Reinius B, Torrens-Juaneda V, Bialecka M, et al. Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells. Nature Communications 2018; 9(1):1-10.
30
Xuemei L, Jing Y, Bei X, Juan H, Xinling R, Qun L, et al. Retinoic acid improve germ cell differentiation from human embryonic stem cells. Iran J Reprod Med 2013; 11(11):905-12.
31
Conrad S, Azizi H, Hatami M, Kubista M, Bonin M, Hennenlotter J, et al. Differential gene expression profiling of enriched human spermatogonia after short-and long-term culture. Biomed Res Int 2014; 2014:138350.
32
Azizi H, Skutella T, Shahverdi A. Generation of mouse spermatogonial stem-cell-colonies in a non-adherent culture. Cell J 2017; 19(2):238-49.
33
Bahmanpour S, Zarei Fard N, Talaei‐Khozani T, Hosseini A, Esmaeilpour T. Effect of BMP 4 preceded by retinoic acid and co‐culturing ovarian somatic cells on differentiation of mouse embryonic stem cells into oocyte‐like cells. Dev Growth Differ 2015; 57(5):378-88.
34
Mansouri V, Salehi M, Nourozian M, Fadaei F, Farahani RM, Piryaei A, Delbari A. The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells. Genetics and molecular biology. 2015;38(2):220-6.
35
Sun YC, Cheng SF, Sun R, Zhao Y, Shen W. Reconstitution of gametogenesis in vitro: meiosis is the biggest obstacle. J Genet Genomics 2014; 41(3):87-95.
36
Gomes Fernandes M, Bialecka M, Salvatori DC, Chuva de Sousa Lopes SM. Characterization of migratory primordial germ cells in the aorta-gonad-mesonephros of a 4.5-week-old human embryo: a toolbox to evaluate in vitro early gametogenesis. Molecular Human Reproduction 2018; 24(5):233-43.
37
ORIGINAL_ARTICLE
Oral Lichen Planus and Celiac Disease: is there any Relationship?
Background: Lichen planus is an autoimmune disorder and is associated with other autoimmune diseases. There is, however, little evidence of the association of oral lichen planus with celiac disease. The aim of this work was to investigate, for the first time, such an association in patients in the city of Mashhad, Iran. Methods: This case-control study was performed during October 2017 to March 2018 in the department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Mashhad University of Medical Sciences, in Iran. All participants were evaluated for Anti-TTG (IgA) and Total IgA, and in some cases for Anti-TTG IgG. Data were analyzed using SPSS software v.20. Results: A total of 96 subjects were considered in the study; 32 in the case group, and the rest in the control group. The mean value of Anti-TTG IgA was 0.12 ± 1.51 Au/ml in the oral lichen planus group, while it was 0.57 ± 1.20 Au/ml in the control group with no significant difference (P=0.167). The mean value of the Total IgA was 134.96 ± 42.86 mg/dl in the lichen planus group, and it was 129.85 ± 55.28 mg/dl in the control group, as they differ negligibly either (P=0.639). Moreover, celiac disease was not present in the population. Conclusions: We showed that there was no celiac disease present in the oral lichen planus patients as well as healthy subjects. Further studies are required to imply or to rule out the association of oral lichen planus and celiac disease.
https://jkmu.kmu.ac.ir/article_91019_5e26bd8738a34f2e5c150b3d5eb7b8d3.pdf
2020-07-01
329
337
10.22062/jkmu.2020.91019
celiac disease
Oral lichen planus
Tissue transglutaminase antibody
Atessa
Pakfetrat
pakfetrata@mums.ac.ir
1
Professor of Oral and Maxillofacial Medicine, Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Zohreh
Dalirsani
dalirsaniz@mums.ac.ir
2
Associate Professor of Oral and Maxillofacial Medicine, Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Hooman
Mosannen Mozaffari
3
Assistant Professor of Gastroenterology and Hepatology, Gastroenterology and Hepatology Department, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mina
Sheikhveysi
sheikhveysim90@gmail.com
4
Oral and Maxillofacial Medicine Specialist, Oral and Maxillofacial Medicine Department, Faculty of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran
LEAD_AUTHOR
Lodi G, Scully C, Carrozzo M, Griffiths M, Sugerman PB, Thongprasom K. Current controversies in oral lichen planus: report of an international consensus meeting. Part 2. Clinical management and malignant transformation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 100(2):164-78.
1
Erickson BA, Elliott SP, Myers JB, Voelzke BB, Smith 3rd TG, McClung CD, et al. Understanding the relationship between chronic systemic disease and lichen sclerosus urethral strictures. J Urol 2016; 195(2):363-8.
2
Kurago ZB. Etiology and pathogenesis of oral lichen planus: an overview. Oral Surg Oral Med Oral Pathol Oral Radiol 2016; 122(1):72-80.
3
Robledo-Sierra J, Landin-Wilhelmsen K, Nyström HF, Mattsson U, Jontell M. Clinical characteristics of patients with concomitant oral lichen planus and thyroid disease. Oral Surg Oral Med Oral Pathol Oral Radiol 2015; 120(5):602-8.
4
Carrozzo M. How common is oral lichen planus? Evid Based Dent 2008; 9(4):112-3.
5
Gupta S, Jawanda MK. Oral lichen planus: an update on etiology, pathogenesis, clinical presentation, diagnosis and management. Indian J Dermatol 2015; 60(3):222-9.
6
Rashid M, Zarkadas M, Anca A, Limeback H. Oral manifestations of celiac disease: a clinical guide for dentists. J Can Dent Assoc 2011; 77(b39).
7
Ruiz Villaverde R, Blasco Melguizo J, Menéndez García Estrada A, Díez García F. Erosive mucosal lichen associated to hyper IgE syndrome and coeliac disease. An Pediatr (Barc) 2004; 60(3):281-2. [In Spanish].
8
Compilato D, Carroccio A, Campisi G. Hidden coeliac disease in patients suffering from oral lichen planus. J Eur Acad Dermatol Venereol 2012; 26(26):390-1.
9
Fortune F, Buchanan J. Oral lichen planus and coeliac disease. Lancet 1993; 341(8853):1154-5.
10
Acar S, Yetkıner AA, Ersın N, Oncag O, Aydogdu S, Arıkan C. Oral findings and salivary parameters in children with celiac disease: a preliminary study. Med Princ Pract 2012; 21(2):129-33.
11
Muñoz F, Del Río N, Sóñora C, Tiscornia I, Marco A, Hernández A. Enamel defects associated with coeliac disease: putative role of antibodies against gliadin in pathogenesis. Eur J Oral Sci 2012; 120(2):104-12.
12
Green PH, Rostami K, Marsh MN. Diagnosis of coeliac disease. Best Practice & Research Clinical Gastroenterology 2005; 19(3):389-400.
13
Cigic L, Gavic L, Simunic M, Ardalic Z, Biocina-Lukenda D. Increased prevalence of celiac disease in patients with oral lichen planus. Clin Oral Investig 2015; 19(3):627-35.
14
Rodrigo L, Beteta-Gorriti V, Alvarez N, Gómez de Castro C, de Dios A, Palacios L, et al. Cutaneous and Mucosal Manifestations Associated with Celiac Disease. Nutrients 2018; 10(7):800.
15
Goddard CJ, Gillett HR. Complications of coeliac disease: are all patients at risk? Postgrad Med J 2006; 82(973):705-12.
16
Nejad MR, Rostami K, Emami MH, Zali MR, Malekzadeh R. Epidemiology of celiac disease in Iran: a review. Middle East J Dig Dis 2011; 3(1):5-12.
17
Pakfetrat A, Javadzadeh-Bolouri A, Basir-Shabestari S, Falaki F. Oral lichen planus: a retrospective study of 420 Iranian patients. Med Oral Patol Oral Cir Bucal 2009; 14(7):E315-8.
18
Thongprasom K, Carrozzo M, Furness S, Lodi G. Interventions for treating oral lichen planus. Cochrane Database Syst Rev 2011; 7:CD001168.
19
Losurdo G, Principi M, Iannone A, Amoruso A, Ierardi E, Di Leo A, et al. Extra-intestinal manifestations of non-celiac gluten sensitivity: an expanding paradigm. World J Gastroenterol 2018; 24(14):1521-30.
20
Catassi C, Bai JC, Bonaz B, Bouma G, Calabrò A, Carroccio A, et al. Non-celiac gluten sensitivity: the new frontier of gluten related disorders. Nutrients 2013; 5(10):3839-53.
21
Catassi C, Elli L, Bonaz B, Bouma G, Carroccio A, Castillejo G, et al. Diagnosis of non-celiac gluten sensitivity (NCGS): the Salerno experts’ criteria. Nutrients 2015; 7(6):4966-77.
22
Spencer J, Sollid LM. The human intestinal B-cell response. Mucosal Immunol 2016; 9(5):1113-24.
23
Jabri B, Sollid LM. Mechanisms of disease: immunopathogenesis of celiac disease. Nat Clin Pract Gastroenterol Hepatol 2006; 3(9):516-25.
24
Green PH, Jabri B. Celiac Disease. Annu Rev Med 2003; 57:207-21.
25
Pastore L, Carroccio A, Compilato D, Panzarella V, Serpico R, Muzio LL. Oral manifestations of celiac disease. J Clin Gastroenterol 2008; 42(3):224-32.
26
Scully C, Porter SR, Eveson JW. Oral lichen planus and coeliac disease. Lancet 1993; 341(8861):1660.
27
Jokinen J, Peters U, Mäki M, Miettinen A, Collin P. Celiac sprue in patients with chronic oral mucosal symptoms. J Clin Gastroenterol 1998; 26(1):23-6.
28
Compilato D, Carroccio A, Campisi G. Hidden coeliac disease in patients suffering from oral lichen planus. J Eur Acad Dermatol Venereol 2012; 26(3):390-1.
29
Nosratzehi T. Oral lichen planus: an overview of potential risk factors, biomarkers and treatments. Asian Pac J Cancer Prev 2018; 19(5):1161-7.
30
ORIGINAL_ARTICLE
Effect of Troxerutin on Oxidative Stress Induced by Sciatic Nerve Ischemia-reperfusion Injury in Rats
Background:Troxerutin has antioxidant and anti-inflammatory properties and in this study, its antioxidant effect on the reduction of oxidative stress induced by ischemia-reperfusion sciatic nerve injury was investigated. Methods:In this study, 64 male rats were randomly divided into 8 groups as follows: 1- IR2: ischemia (3 hours) and reperfusion (2 days), 2- Trox+IR2: ischemia (3 hours) and reperfusion (2 days), 3- IR7: ischemia (3 hours) and reperfusion (7 days), 4- Trox+IR7: ischemia (3 hours) and reperfusion (7 days), 5- IR14: ischemia (3 hours) and reperfusion (14 days), 6- Trox+IR14: ischemia (3 hours) and reperfusion (14 days), 7- IR28: ischemia (3 hours) and reperfusion (28 days), 8- Trox+IR28: ischemia (3 hours) and reperfusion (28 days). The rats received 150 mg/kg troxerutin in one injection (single dose). After separation of serum, biochemical parameters of the serums such as NO, PON1, CAT, and GPX were measured. Results:Troxerutin significantly increased the GPX and PON1 levels in groups that their reperfusion time was 2 and 14 days (p <0.05). There was no significant difference in the levels of NO and CAT between the groups received troxerutin and control groups (P>0.05). Conclusion:Troxerutin relatively decreased the oxidative stress in the sciatic nerve ischemia-reperfusion injury by increasing the level of antioxidant enzymes.
https://jkmu.kmu.ac.ir/article_91020_6b31884859dced42598b8bc25402c402.pdf
2020-07-01
338
347
10.22062/jkmu.2020.91020
Troxerutin Sciatic Nerve Ischemia
Reperfusion Rat
Saideh
Dadpisheh
s.dadpisheh@gmail.com
1
M.Sc. of Physiology, Department of Physiology, Iranshahr University of Medical Sciences, Iranshahr, Iran
AUTHOR
Hassan
Ahmadvand
hassan_a46@yahoo.com
2
Professor in Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
AUTHOR
Leila
Jafaripour
elahejafari62@gmail.com
3
MSc. of Anatomical Scinces, Department of Anatomy, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran and Department of Anatomical Sciences, Afzalipour Medical School, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Negar
Nouryazdan
negar.noury@gmail.com
4
M.Sc. of Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
AUTHOR
Esmaeel
Babaeenezhad
es.babaeenezhad1391@gmail.com
5
Ph.D. Student in Clinical Biochemistry, Department of Clinical Biochemistry, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Hamzeh
Shati
hamzehshati@gmail.com
6
Student of Medicine, Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
AUTHOR
Shahrokh
Bagheri
sbg1660@yahoo.com
7
Ph.D. Student in Clinical Biochemistry, Razi Herbal Medicines Researches Center, Lorestan University of Medical Sciences, Khorramabad, Iran
LEAD_AUTHOR
McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 1985; 312(3):159-63.
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Maurya DK, Salvi VP, Nair CK. Radioprotection of normal tissues in tumor-bearing mice by troxerutin. J Radiat Res 2004; 45(2):221-8.
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Vinothkumar R, Vinoth Kumar R, Karthikkumar V, Viswanathan P, Kabalimoorthy J, Nalini N. Oral supplementation with troxerutin (trihydroxyethylrutin), modulates lipid peroxidation and antioxidant status in 1, 2-dimethylhydrazine-induced rat colon carcinogenesis. Environ Toxicol Pharmacol 2014; 37(1):174-84.
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Kessler M, Ubeaud G, Walter T, Sturm F, Jung L. Free radical scavenging and skin penetration of troxerutin and vitamin derivatives. J Dermatolog Treat 2002; 13(3):133-41.
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Saray A, Apan A, Kisa U. Free radical-induced damage in experimental peripheral nerve injection injury. J Reconstr Microsurg 2003; 19(6):401-6.
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Tokmak M, Sehitoglu MH, Yuksel Y, Guven M, Akman T, Aras AB, et al. The axon protective effects of syringic acid on ischemia/reperfusion injury in a rat sciatic nerve model. Turk Neurosurg 2017; 27(1):124-32.
18
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Kandhare AD, Mukherjee AA, Bodhankar SL. Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: Evidence from anti-inflammatory, antioxidant and anti-apoptotic studies. EXCLI J 2017; 16:546-65.
22
Gholami M, Khanipour Khayat Z, Anbari K, Obidavi Z, Varzi A, Boroujeni MB, et al. Quercetin ameliorates peripheral nerve ischemia–reperfusion injury through the NF-kappa B pathway. Anat Sci Int 2017; 92(3):330-7.
23
Zhang MT, Wang B, Jia YN, Liu N, Ma PS, Gong SS, et al. Neuroprotective effect of liquiritin against neuropathic pain induced by chronic constriction injury of the sciatic nerve in mice. Biomedicine & Pharmacotherapy 2017; 95:186-98.
24
Guven M, Gölge UH, Aslan E, Sehitoglu MH, Aras AB, Akman T, et al. The effect of aloe vera on ischemia—Reperfusion injury of sciatic nerve in rats. Biomed Pharmacother 2016; 79:201-7.
25
Yu Y, Zheng G. Troxerutin protects against diabetic cardiomyopathy through NF‑κB/AKT/IRS1 in a rat model of type 2 diabetes. Mol Med Rep 2017; 15(6):3473-8.
26
Najafi M, Noroozi E, Javadi A, Badalzadeh R. Anti-arrhythmogenic and anti-inflammatory effects of troxerutin in ischemia/reperfusion injury of diabetic myocardium. Biomed Pharmacother 2018; 102:385-91.
27
Badalzadeh R, Chodari L, Ghorbanzadeh V. Troxerutin, a bioflavonoid, improves oxidative stress in blood of streptozotocin-induced type-1 diabetic rats. Iranian Journal of Pharmaceutical Sciences 2017; 13(2):75-86.
28
Elangovan P, Ramakrishnan R, Amudha K, Jalaludeen AM, Sagaran GK, Babu FR, et al. Beneficial Protective Effect of Troxerutin on Nickel-Induced Renal Dysfunction in Wistar Rats. J Environ Pathol Toxicol Oncol 2018; 37(1):1-14.
29
Raja B, Saranya D, Prabhu R. Role of flavonoid troxerutin on blood pressure, oxidative stress and regulation of lipid metabolism. Front Biosci (Elite Ed) 2019; 11:121-9.
30
Jalali M, Hassanipour M, Hajizadeh M, Khanamani Falahati Pour S, Khoshdel A, Roustai F, et al. Troxerutin chronic treatment protects against fructose-induced metabolic syndrome in male rats. International Journal of Medical Laboratory 2017; 4(3):201-10.
31
Costa LG, Cole TB, Garrick JM, Marsillach J, Furlong CE. Metals and paraoxonases. Adv Neurobiol 2017; 18:85-111.
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Farajdokht F, Amani M, Mirzaei Bavil F, Alihemmati A, Mohaddes G, Babri S. Troxerutin protects hippocampal neurons against amyloid beta-induced oxidative stress and apoptosis. EXCLI J 2017; 16:1081-9.
33
Thomas NS, George K, Arivalagan S, Mani V, Siddique AI, Namasivayam N. The in vivo antineoplastic and therapeutic efficacy of troxerutin on rat preneoplastic liver: biochemical, histological and cellular aspects. Eur J Nutr 2017; 56(7):2353-66.
34
ORIGINAL_ARTICLE
Effects of Boswellia serrata on Improvement of Memory Impairment in Patients with Mild Cognitive Impairment: A Double-blind, Randomized, Placebo-controlled Study
Background: Mild cognitive impairment (MCI) is the stage between the expected cognitive decline of normal aging and the more serious decline of dementia. In the present study, the effect of Boswellia serrata (BS) on improvement of memory impairment in patients with MCI was investigated. Methods: In this single-center randomized double-blind placebo-controlled clinical trial study, 118 patients with mild cognitive impairment (MCI) were included and randomly divided into two groups (case and control). Control group (n=59) received BS 300 mg/kg body weight twice a day and control group (n=59) received placebo for a period of three and six months. Montreal Cognitive Assessment (MoCA) test for detecting cognitive impairment was done at baseline, three and six months after the intervention. Results: A significant difference was reported in the MoCA mean score between the groups after three months (24.64 vs. 22.83) and six months of the intervention (25.22 vs. 22.7). Memory item had the greatest impact on the average final score (P≤0.0001). Conclusion: BS has a significant effect on the improvement of memory impairment in patients with MCI. Further studies are required with higher doses of BS and longer duration of treatment to assess the effects of BS on memory of patients with MCI.
https://jkmu.kmu.ac.ir/article_91021_ce12fecc975275b853c9e7f27588ea1c.pdf
2020-07-01
348
355
10.22062/jkmu.2020.91021
Traditional Iranian Medicine (TIM)
Mild Cognitive Impairment (MCI)
Boswellia serrata
Dementia
Soheila
Rezakhani
drrezakhani@gmail.com
1
Assistant Professor, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Ir
AUTHOR
Behnaz
Sedighi
behnaz.sedighi@gmail.com
2
Associate Professor, Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran
LEAD_AUTHOR
Abbas
Pardakhty
abpardakhty@kmu.ac.ir
3
Professor, Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Kaveh
Shafiee
4
Assistant Professor, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Victor M, Adams R, Collins G. Mild Cognitive Impairment. 10th ed. Philadelphia: Davis; 2014.
1
Petersen RC. Mild cognitive impairment. Continuum (Minneap Minn) 2016; 22(2 Dementia):404-18.
2
Freitas S, Simões MR, Alves L, Santana I. Montreal cognitive assessment: influence of sociodemographic and health variables. Arch Clin Neuropsychol 2012; 27(2):165-75.
3
Feldman HH, Ferris S, Winblad B, Sfikas N, Mancione L, He Y, et al. Effect of rivastigmine on delay to diagnosis of Alzheimer's disease from mild cognitive impairment: the InDDEx study. Lancet Neurol 2007; 6(6):501-12.
4
Singh GB, Singh S, Bani S. Anti-inflammatory actions of boswellic acids. Phytomedicine 1996; 3(1):81-5.
5
Sharma R, Singh S, Singh GD, Khajuria A, Sidiq T, Singh SK, et al. In vivo genotoxicity evaluation of a plant based antiarthritic and anticancer therapeutic agent Boswelic acids in rodents. Phytomedicine 2009; 16(12):1112-8.
6
Safayhi H, Mack T, Sabieraj J, Anazodo MI, Subramanian LR, Ammon HP. Boswellic acids: novel, specific, nonredox inhibitors of 5-lipoxygenase. J Pharmacol Exp Ther 1992; 261(3):1143-6.
7
Farshchi A, Ghiasi G, Farshchi S, Malek Khatabi P. Effects of boswellia papyrifera gum extract on learning and memory in mice and rats. Iran J Basic Med Sci 2010; 13(2):9-15.
8
Karima O, Riazi G, Yousefi R, Movahedi AA. The enhancement effect of beta-boswellic acid on hippocampal neurites outgrowth and branching (an in vitro study). Neurol Sci 2010; 31(3):315-20.
9
Mahmoudi A, Hosseini-Sharifabad A, Monsef-Esfahani HR, Yazdinejad AR, Khanavi M, Roghani A, et al. Evaluation of systemic administration of Boswellia papyrifera extracts on spatial memory retention in male rats. J Nat Med 2011; 65(3-4):519-25.
10
Hosseini Sharifabad M, Esfandiary E. A morphometeric study on CA3 hippocampal field in young rats following maternal administration of Boswellia serrata resin during gestation. Iranian Journal of Basic Medical Sciences 2007; 10(3):176-82.
11
Nasreddine ZS, Phillips NA, Bédirian V, Charbonneau S, Whitehead V, Collin I, et al. The montreal cognitive assessment, MoCA: a brief screening tool for mild cognitive impairment. J Am GeriatrSoc 2005; 53(4):695-9.
12
Smith T, Gildeh N, Holmes C. The montreal cognitive assessment: validity and utility in a memory clinic setting. Can J Psychiatry 2007; 52(5):329-32.
13
Petersen RC, Thomas RG, Grundman M, Bennett D, Doody R, Ferris S, et al. Vitamin E and donepezil for the treatment of mild cognitive impairment. N Engl J Med 2005; 352(23):2379-88.
14
Beck AT, Epstein N, Brown G, Steer RA. An inventory for measuring clinical anxiety: psychometric properties. J Consult ClinPsychol 1988; 56(6):893-7.
15
Basch E, Boon H, Heerema TD, Foppo I, Hashmi S, Hasskarl J, et al. Boswellia: An evidence-based systematic review by the natural standard research collaboration. J Herb Pharmacother 2004; 4(3):63-83.
16
Paul R, Lane EM, Tate DF, Heaps J, Romo DM, Akbudak E, et al. Neuroimaging signatures and cognitive correlates of the Montreal cognitive assessment screen in a nonclinical elderly sample. Arch Clin Neuropsychol 2011; 26(5):454-60.
17
Peila R, Launer LJ. Inflammation and dementia: epidemiologic evidence. Acta Neurol Scand Suppl 2006; 185:102-6.
18
Karplus TM, Saag KG. Nonsteroidal anti-inflammatory drugs and cognitive function. Drug Saf 1998; 19(6):427-33.
19
Poeckel D, Werz O. Boswellic acids: biological actions and molecular targets. Curr Med Chem 2006; 13(28):3359-69.
20
Rainer E. Use of Francincence (olibanum) in the treatment of alzhaimer s disease. Chem ABS 1996; 125:1327-94.
21
Sedighi B, Pardakhty A, Kamali H, Shafiee K, Hasani BN. Effect of Boswellia papyrifera on cognitive impairment in multiple sclerosis. Iran J Neurol 2014; 13(3):149-53.
22
Gupta I, Parihar A, Malhotra P, Singh GB, Lüdtke R, Safayhi H, et al. Effects of Boswellia serrata gum resin in patients with ulcerative colitis. Eur J Med Res 1997; 2(1):37-43.
23
Gupta I, Parihar A, Malhotra P, Gupta S, Lüdtke R, Safayhi H, et al. Effects of gum resin of Boswellia serrata in patients with chronic colitis. Planta Med 2001; 67(5):391-5.
24
Gerhardt H, Seifert F, Buvari P, Vogelsang H, Repges R. Therapy of active Crohn disease with Boswellia serrata extract H 15. Z Gastroenterol 2001; 39(1):11-7. [In German].
25
Qiu C, De Ronchi D, Fratiglioni L. The epidemiology of the dementias: an update. Curr Opin Psychiatry 2007; 20(4):380-5.
26
Taghizadeh M, Maghaminejad F, Aghajani M, et al. The effect of tablet containing Boswellia serrata and Melisa officinalis extract on older adults' memory: A randomized controlled trial. Archives of Gerontology and Geriatrics 2017; 75: 146-150.
27
ORIGINAL_ARTICLE
Live Birth Rate following Intrauterine Insemination in Women with Low or Very Low Level of Serum Anti-müllerian Hormone
Background: While anti-Müllerian hormone (AMH) level allows quantitative evaluation of ovarian reserve, its predictive value for live births following assisted reproductive technology cycles has remained controversial. The aim of the present study was to assess the importance of AMH in predicting live birth following intrauterine insemination (IUI) in the case of low or very low ovarian reserve. Methods: In this retrospective cohort study, 123 patients with AMH≤1 ng/ml, who underwent a total of 137 IUI cycles were enrolled and evaluated for live birth rate. Patients were divided into two groups based on serum AMH levels: group 1 with low level of AMH (0.4-1 ng/ml, n=83, cycles: 95) and group 2 with very low level of AMH (≤0.4 ng/ml, n=40, cycles: 42). The results were compared between the two groups. Main outcome was the pregnancy rate. Results: The rates of biochemical pregnancy, clinical pregnancy and live birth in all patients were 11%, 8% and 7.3%, respectively. The two groups showed no significant difference in the rates of biochemical pregnancy (10.4% vs. 14.3%, p=0.3), clinical pregnancy (6.3% vs. 11.9%, p=0.2) and live birth (6.3% vs. 9.8%, p=0.5). In univariate regression analysis, baseline characteristics and ovarian stimulation parameters showed no significant relationship with the rates of pregnancy and live birth. Conclusion: In women with AMH≤1 ng/ml, serum levels of AMH did not appear to reflect pregnancy outcomes and live births following IUI. It can be concluded that in women with low or very low levels of AMH, there is chance of pregnancy, and live birth following IUI.
https://jkmu.kmu.ac.ir/article_91022_57c091d9de5d3cb9de2cb6dd612d9a8b.pdf
2020-07-01
356
361
10.22062/jkmu.2020.91022
Anti
müllerian hormone Intrauterine insemination Live birth Assisted reproductive technology
Marzieh
Mehrafza
marzieh.mehrafza@gmail.com
1
Obstetrician and Gynecologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
LEAD_AUTHOR
Tahereh
Zare Yousefi
t.z.yousefi@gmail.com
2
Obstetrician and Gynecologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
AUTHOR
Sahar
Saghati Jalali
saharjalali2008@yahoo.com
3
Obstetrician and Gynecologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
AUTHOR
Azadeh
Raoufi
az.raoufi@gmail.com
4
Developmental Biologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
AUTHOR
Elmira
Hosseinzadeh
elmira.hosseinzadeh@yahoo.com
5
Embryologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
AUTHOR
Sajedeh
Samadnia
s.samadnia@gmail.com
6
Statistician, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
AUTHOR
Maliheh
Habibdoost
maliheh.habibdoost@gmail.com
7
Midwife, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
AUTHOR
Ahmad
Hosseini
8
Professor, Embryologist, Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
AUTHOR
La Marca A, Sighinolfi G, Radi D, Argento C, Baraldi E, Artenisio AC, et al. Anti-Müllerian hormone (AMH) as a predictive marker in assisted reproductive technology (ART). Hum Reprod Update 2010; 16(2):113-30.
1
Nardo LG, Gelbaya TA, Wilkinson H, Roberts SA, Yates A, Pemberton P, et al. Circulating basal anti-Müllerian hormone levels as predictor of ovarian response in women undergoing ovarian stimulation for in vitro fertilization. Fertil Steril 2009; 92(5):1586-93.
2
Riggs R, Kimble T, Oehninger S, Bocca S, Zhao Y, Leader B, et al. Anti-Müllerian hormone serum levels predict response to controlled ovarian hyperstimulation but not embryo quality or pregnancy outcome in oocyte donation. Fertil Steril 2011; 95(1):410-2.
3
Tremellen K, Kolo M. Serum anti‐Mullerian hormone is a useful measure of quantitative ovarian reserve but does not predict the chances of live‐birth pregnancy. Aust N Z J Obstet Gynaecol 2010; 50(6):568-72.
4
La Marca A, Giulini S, Tirelli A, Bertucci E, Marsella T, Xella S, et al. Anti-Müllerian hormone measurement on any day of the menstrual cycle strongly predicts ovarian response in assisted reproductive technology. Hum Reprod 2006; 22(3):766-71.
5
Gleicher N, Weghofer A, Barad DH. Anti-Müllerian hormone (AMH) defines, independent of age, low versus good live-birth chances in women with severely diminished ovarian reserve. Fertil Steril 2010; 94(7):2824-7.
6
Weghofer A, Dietrich W, Barad DH, Gleicher N. Live birth chances in women with extremely low-serum anti-Mullerian hormone levels. Hum Reprod 2011; 26(7):1905-9.
7
Kedem A, Haas J, Geva LL, Yerushalmi G, Gilboa Y, Kanety H, et al. Ongoing pregnancy rates in women with low and extremely low AMH levels. A multivariate analysis of 769 cycles. PLoS One 2013; 8(12):e81629.
8
Reijnders IF, Nelen WL, IntHout J, van Herwaarden AE, Braat DD, Fleischer K. The value of Anti-Müllerian hormone in low and extremely low ovarian reserve in relation to live birth after in vitro fertilization. Eur J Obstet Gynecol Reprod Biol 2016; 200:45-50.
9
Łukaszuk K, Kunicki M, Liss J, Bednarowska A, Jakiel G. Probability of live birth in women with extremely low anti-Müllerian hormone concentrations. Reprod Biomed Online 2014; 28(1):64-9.
10
Freiesleben N, Rosendahl M, Johannsen TH, Løssl K, Loft A, Bangsbøll S, et al. Prospective investigation of serum anti-Müllerian hormone concentration in ovulatory intrauterine insemination patients: a preliminary study. Reprod Biomed Online 2010; 20(5):582-7.
11
Li HW, Yeung WS, Lau EY, Ho PC, Ng EH. Evaluating the performance of serum antimullerian hormone concentration in predicting the live birth rate of controlled ovarian stimulation and intrauterine insemination. Fertil Steril 2010; 94(6):2177-81.
12
Lie Fong S, Baart EB, Martini E, Schipper I, Visser JA, Themmen AP, et al. Anti-Müllerian hormone: a marker for oocyte quantity, oocyte quality and embryo quality? Reprod Biomed Online 2008; 16(5):664-70.
13
Smeenk JM, Sweep FC, Zielhuis GA, Kremer JA, Thomas CM, Braat DD. Antimüllerian hormone predicts ovarian responsiveness, but not embryo quality or pregnancy, after in vitro fertilization or intracyoplasmic sperm injection. Fertil Steril 2007; 87(1):223-6.
14
Koo HS, Song IO, Cha SH, Park CW, Kim HO. The likelihood of achieving pregnancy through timed coitus in young infertile women with decreased ovarian reserve. Clin Exp Reprod Med 2018; 45(1):31-7.
15
Wang MH, Chen CH, Wang CW, Hsu MI, Tzeng CR. A higher anti-Müllerian hormone level is associated with an increased chance of pregnancy in patients undergoing controlled ovarian stimulation and intrauterine insemination. J Obstet Gynaecol 2015; 35(1):64-8.
16
ORIGINAL_ARTICLE
Node-First Kawasaki Disease Presented with Marked Pancarditis: a Case Report
Kawasaki disease is an acute inflammatory disorder of medium-sized arteries that predominantly affects cardiac coronary arteries and children under the age of 5 years. Cardiac involvement usually happens later than 10 days after the onset of illness. Most of cardiac complications are coronary artery abnormalities (ectasia or aneurysms) and subclinical myocarditis. Clinical myocarditis (symptomatic congestive heart failure), pericarditis, valvulitis and pericardial effusion, as well as pancarditis are rare. This paper reports a 5-year-old boy who had heart failure (ejection fraction 48%) in the acute stage of Kawasaki disease and pericarditis. He was admitted to the hospital following 3 days of continuous fever, bilateral cervical adenopathy and dominant right side neck of torticollis. The results of physical examination after 5 days showed typical Kawasaki disease. Cardiac examination also revealed cardiac murmur and gallop rhythm. In laboratory tests, mild liver dysfunction, hypoproteinemia and hyponatremia were discovered. During hospitalization, troponin levels were positive. The patient was treated with oral high dose aspirin (100 mg/kg/d), two doses of intravenous immunoglobulin (IVIG 2 gm/kg) and three pulses of methylprednisolone. Two weeks later, cardiac evolvements were improved without further complications. The patient exhibited dramatically clinical recovery and was healthy after 8 weeks of follow-up. This case indicates that Lymph-node-first presentation of Kawasaki disease could be examined in children with Kawasaki disease who exhibit symptoms of congestive cardiac failure, pericardial effusion and pericarditis during the acute phase of the disease.
https://jkmu.kmu.ac.ir/article_91023_c152abf4886dfe8a226fcb4aefb0cacc.pdf
2020-07-01
362
368
10.22062/jkmu.2020.91023
Cervical adenopathy
Congestive heart failure
Kawasaki disease
Myocarditis
Mucocutaneous lymph node syndrome
Pericardial Effusion
Yazdan
ghandi
y.ghandi@arakmu.ac.ir
1
Associate Professor, Amirkabir Hospital, Department of Pediatrics, School of Medicine, Arak University of Medical Sciences, Arak, Iran
LEAD_AUTHOR
Fatemeh
Dorreh
2
Associate Professor, Amirkabir Hospital, Department of Pediatrics, School of Medicine, Arak University of Medical Sciences, Arak, Iran
AUTHOR
Roghayeh
Ahmadi
3
Amirkabir Hospital, School of Medicine, Arak University of Medical Sciences, Arak, Iran
AUTHOR
Danial
Habibi
dhabibi67@gmail.com
4
Ph.D. candidate, Department of Biostatistics, Arak University of Medical Sciences, Arak, Iran
AUTHOR
Nomura Y, Arata M, Koriyama C, Masuda K, Morita Y, Hazeki D, et al. A severe form of Kawasaki disease presenting with only fever and cervical lymphadenopathy at admission. J Pediatr 2010; 156(5):786-91.
1
Kanegaye JT, Van Cott E, Tremoulet AH, Salgado A, Shimizu C, Kruk P, et al. Lymphnodefirst presentation of Kawasaki disease compared with bacterial cervical adenitis and typical Kawasaki disease. J Pediatr 2013; 162(6):1259-63.
2
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