Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
19
6
2012
02
01
Evaluation of Nucleostemin Gene Expression as a New Molecular Marker in Breast Tumors
113
125
EN
M.A
Hoseinporfeizi
Professor of Radiobiology, School of Natural Sciences, Tabriz University, Tabriz, Iran
S
Saed
. M.Sc. in Genetics, Department of Biology, School of Natural Sciences, Tabriz University, Tabriz, Iran
E
Babaei
Ph.D. student of Molecular Genetics, School of Natural Sciences, Tarbiat Modares University, Tehran, Iran
V
Montazeri
Professor of Thorax Surgery, Department of Surgery, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
M
Halimi
Assistant Professor of Pathology, Department of Pathology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Background & Aims: Nucleostemin is one of the stem cell enriched proteins which encodes a novel nucleolar GTP-binding protein found at high levels in the adult and embryonic stem (ES) cells but not in terminally differentiated cells. It is also expressed in tumor cell lines as well as in the several types of human cancers. Due to the increasing rate of breast cancer in recent years, in the present study we evaluate the usefulness of Nucleostemin as a potential diagnostic and therapeutic molecular marker in breast tumors. Methods: A total of 41 tumoral and 20 non-tumoral adjacent tissues were studied by Semiquantitative Reverse Transciptase-Polymerase Chain Reaction (RT-PCR). 2m was used as an internal control. Data were analyzed through SPSS software. Results: According to the obtained results, nucleostemin is a proliferation marker with higher eapression in breast tumors rather than in adjacent normal tissues. Nucleostemin expression level was significantly correlated with profilertion potential of breast benign tumors (p< 0.05). The expression of Nucleostemin was significantly correlated with the advanced stages of breast tumors (p<0.05). Conclusion: Nucleostemin expression level may be used in estimating tumor size and as a potential prognostic marker for determinig breast tumors stage and future metastases. Moreover, nucleostemin inhibition can be an effective sterategy in decreasing the proliferation of breast tumor cell lines
Nucleostemin,Breast Neoplasms,Reverse Transcriptace Polimeras Chain Reaction (RT-PCR)
https://jkmu.kmu.ac.ir/article_16531.html
https://jkmu.kmu.ac.ir/article_16531_d8fbdff27e701a61e0dcc6598ad6718e.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
19
6
2012
02
01
Measurement of Left Ventricular Myocardium Wall Instantaneous Motions with Echocardiographic Sequence Images
126
139
EN
Z
Arabbafrani
Postgraduate Student of Medical Physics, Tarbiat Modares University, Tehran, Iran
M
Mokhtaridezji
Professor of Medical Physics, Trabiat Modares University, Tehran, Iran
F
Roshanali
Cardiologist, Dey Hospital, Tehran, Iran
E
Emamdaditaremi
Postgraduate Student of Computer Engineering and Information Technology, Amir Kabir University of Technology, Tehran, Iran
Background & Aims: One of the important aims of quantitative cardiac image processing is the clarification of myocardial motions in order to derive biomechanical behavior of the heart in the disease condition. In this study we presented a computerized analysis method for detecting the instantaneous myocardial changes by using 2D echocardiography images. Methods: The analysis was performed on the myocardial septum wall of 10 healthy participants in longitudinal and short axis views. Myocardial muscle's vertical and horizontal displacements in the basal and apex segments were also estimated. In order to detemin the validity of the planned program, manual measurements were performed too and the results of automatic and manual methods were compared. Pearson's test used to find out the correlation between manual and automatic methods and the linear correlation function between these two methods was extracted by the confidence level of 95%. Results: There was significant correlation between the displacements of the base and apex segments of the interventricular septum acquired using block matching and manual methods (R2= 0.91, p<0.05). In studying the difference between the two methods based on their average, significant correlation between the manual and block maching methods was observed. Maximum displacement of the interventricular septum wall measured by block matching algorithm and manual method showed no significant difference. Conclusion: Since the proposed approach based on block matching is capable of assessing the instantaneous changes of regional myocardial wall in longitudinal and short axes views of sequential B-mode images, it has the potential to be used in clinical practice
echocardiography,Motion detection,Block matching algorithm
https://jkmu.kmu.ac.ir/article_16532.html
https://jkmu.kmu.ac.ir/article_16532_e8e63f85eb8615ab66232b6e1b4b90ba.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
19
6
2012
02
01
Comparison of Fluoride Uptake into Enamel from Sodium Fluoride Gel 0.05% Produced in Iran and Stannous Fluoride 0.4% Gel
140
148
EN
H.R
Poreslami
Associate Professor, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran
P
KHazaeli
Associate Professor, School of pharmacy, Kerman University of Medical Sciences, Kerman, Iran
F.S
Sajadi
Assistant Professor, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran
R
Hasanzadeh
Dentist
Background & Aims: The present study compared fluoride uptake into enamel from sodium fluoride 0.05% gel and American Dental Association approved fluoride gel (Stannous fluoride 0.4% , Sultan Co.) when used on healthy enamel of the intact teeth. Methods: In an experimental study, 30 intact teeth extracted for orthodontic purposes were randomly assigned into two groups of 15 teeth. The teeth were sectioned in two mesial and distal halves as control and experimental sides. Defined semi-circular areas on the enamel of experimental halves were treated with gel for 4 minutes, the halves were stored in artificial saliva for 24 hours at 37 ºC, etched for 30 seconds by 0.5 M perchlorid Acid and washed by 0.2 ml KOH after each etching. Biopsy of the samples was obtained by Acid Etch Enamel Biopsy technique and the fluoride and calcium concentration were calculated by potentiometer and spectrophotometer respectively. Paired t test and student t test were used for statistical analyses. Results: The results showed significant increase of enamel fluoride content in the experimental halves after exposure to both stannous fluoride 0.4% (P=0.0001) and sodium fluoride 0.05% (P=0.009). Mean fluoride uptakes in stannous fluoride 0.4% gel and sodium fluoride 0.05% gel groups were respectively 4052.5 ppm and 892.5 ppm that shows statistically significant difference (P=0.0001). Conclusion: Although both sodium fluoride 0.05% and stannous fluoride 0.4% increased fluoride Content of tooth enamel after application, stannous fluoride 0.4% caused more fluoride uptake into the tooth enamel that is due to its higher amount of fluoride ion (1000 ppm) compared to sodium fluoride gel (225 ppm).
Fluoride,Dental Caries,Prevention,Enamel
https://jkmu.kmu.ac.ir/article_16533.html
https://jkmu.kmu.ac.ir/article_16533_9f23c6aa540c9325dcc8fd95e90f44d8.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
19
6
2012
02
01
Comparing the Effectiveness of Psychological Debriefing, Eye Movement Desensitization Reprocessing, and Imaginal Exposure on Treatment of Chronic Post-traumatic Stress Disorder
149
159
EN
F
KHosropor
Ph.D. in Psychology, Tehran Central Branch, Islamic Azad University, Tehran, Iran
GH.R
Ebrahiminejad
Associate Professor, Department of Psychiatry & Behavior Science Committee, Kerman University of Medical Sciences, Kerman, Iran
M
Salehi
Assistant Professor, Department of Psychology, Tehran Central Branch, Islamic Azad University, Tehran, Iran
V
Farzad
Assistant Professor, Department of Psychology, Tehran Central Branch, Islamic Azad University, Tehran, Iran
Background & Aims: Post-traumatic stress disorder (PTSD) is considered as one of the most prevalent disorder during the life time and can negatively influence the individual, family and social relationships of patients; so, prevention and treatment of this disorder is highly important. Eye movement desensitization and reprocessing (EMDR), psychological debriefing (PD), and imaginal exposure (IE) are some treatment methods; but there is controversy about long effects of these treatments, especially among chronic patients. Method: In a semi experimental study, a total of 54 adult male patients, based on Davidson scale and psychiatric diagnostic, were randomly selected, and then were divided into 3 equal therapy groups. All participants were evaluated before, after and 3 months after the treatment. Data were analyzed through the repeated variance and Duncan post-hoc tests. Results: Psychological debriefing and eye movement desensitization and reprocessing were better than imaginal exposure in relief of chronic post-traumatic stress disorder signs and remaining the effectiveness in three months follow-up. Conclusion: It is concluded that all of the above methods are effective on chronic post-traumatic stress disorder and the efficacy of the therapeutic techniques would be still in force even after 3 months. Considering the importance of psychological interventions, it is necessary that such methods be taught to psychologists so that they can use them after traumatic accidents.
Chronic post-traumatic stress disorder, Eye movement desensitization reprocessing, Debriefing,,exposure therapy
https://jkmu.kmu.ac.ir/article_16534.html
https://jkmu.kmu.ac.ir/article_16534_1ec831077a88dcbc75ccb5027b4d058d.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
19
6
2012
02
01
Comparison of the Efficacy of Vaginal Misoprostol with and without Laminaria in Second Trimester Induction Abortion
160
167
EN
Z
Salari
Assistant Professor of Obstetrics & Gynecology, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
L
SHarifi
Resident of Obstetrics & Gynecology, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
B.SH
Aali
Professor of Obstetrics & Gynecology, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Background & Aims: Therapeutic abortion in the second trimester pregnancy is the choice treatment in cases with severe anomalies or intrauterine fetal death. It can be done by a number of medical (e.g. misoprostol) or mechanical methods (e.g. laminaria). The aim of this study was to compare the efficacy of misoprostol alone and misoprostol in combination with laminaria in the induction of second trimester abortion. Method: In this interventional study, 80 pregnant women with the gestational age of 13-25 weeks who were candidates of therapeutic abortion were selected and randomly divided into two groups (n=40). One group received misoprostol alone and the other received misoprostol in combination with laminaria. Results: Combination of misoprostol and laminaria caused not only a significant shorter time interval from the initiation of intervention to abortion (P=0.034), but also lower dose of administered misoprostol (P=0.001), less need for curettage (P=0.001), and shorter hospitalization period (P = 0.042). Conclusion: Based on the obtained results, misoprostol in combination with laminaria is recommended for the induction of second trimester therapeutic abortion.
Laminaria,misoprostol,Second trimester,Therapeutic abortion
https://jkmu.kmu.ac.ir/article_16535.html
https://jkmu.kmu.ac.ir/article_16535_0d2cc29fbc9252b5c763b436439318ea.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
19
6
2012
02
01
The Prevalence of P53 Mutations in Laryngeal Cancer in Kerman
168
175
EN
M.A
Damghani
Assistant Professor of Ear, Nose and Throat, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
M.R
Bazrafshani
Assistant Professor of Medical Genetics, Physiology Research Center & Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
T,R
Mirshekari
Clinical Pathologist, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran
Background &Aims: Laryngeal cancer is the second common cancer of respiratory tract, following the lung cancer. Carcinogenesis is a complex multistage process; molecular genetics has provided the evidence that activation of proto-oncogene and loss or inactivation of tumor suppressor genes (TSG) are involved in a large number of malignancies. One of the earliest significant tumor suppressor genes identified in head and neck squamous cell carcinoma (HNSCC) was P53 have a role in growth suppression activities. Thus, when P53 is deleted or silenced, the cell develops a selective growth advantage and becomes a cancer. Mutations in P53 are correlated with poorer survival and response to treatment. The aim of this study was to survey the prevalence of P53 gene mutation in patients with laryngeal cancer and to select an appropriate method of treatment. Methods: The samples were 52 patients with laryngeal cancer diagnosis have been treated by surgery. Investigation of TP53 mutation where performed by multiple ligation probe amplification (MLPA) technique which analyze the full length of gene from exon 1 to 12. Results: The TP53 mutation was discovered in 80.8 percent of samples. By contrast between two main forms of mutation (i.e. deletions and duplications), we found that the deletions mostly occurred within exons 1, 3, 6, 9 and 12 by 59.6 percent and duplications observed in exons 1, 2, 7, 8 and 11 by 21.2 percent. Conclusion: Considering our results and reminding this fact that nowadays the definitive diagnosis of laryngeal cancer is made using biopsy and pathology techniques, we suggest that all biopsy specimens should be tested and those confirmed positive for TP53 mutations need some further decisions by physicians.
Mutation,p53,cancer,Multiple ligation probe amplification (MLPA),Larynx
https://jkmu.kmu.ac.ir/article_16536.html
https://jkmu.kmu.ac.ir/article_16536_60a4d72104a29032e2d49dd95bc74f1e.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
19
6
2012
02
01
Validity and Reliability of the Persian Version of JSPE-HP Questionnaire (Jefferson Scale of Physician Empathy-Health Professionals Version)
201
211
EN
M.S
Hashempor
Assistant Professor of Oral Medicine, Oral and Dental Diseases Research Center & School of Dentistry, Kerman University of
Medical Sciences, Kerman, Iran
M.A
Karami
Dentist
Background & Aims: Empathy plays an important role in patient’s relationship with his/her dentist or doctor. One of the most used tools for evaluation of empathy is the Jefferson Scale of Physician EmpathyHealth Professionals Version (JSPE-HP). The aim of this study was to validate the JSPE-HP for use among Iranian population. Methods: The English original version of the JSPE-HP questionnaire was translated into Persian language by a forward–backward translation method. Reliability was tested on 30 Dental and Medical students. Then validity and internal consistency were tested on 554 students. Results: The reliability coefficient (Cronbach's alpha) of the JSPE-HP was excellent (alpha: 0.83). The coefficient of test re-test reliability measured by ICC (Intra class correlation coefficient) was 0.82 (CI 95%: 0.80-0.87). Freshman students had higher scores than other students and this difference was significant (P=0.03). There was no significant difference on empathy scores between Dental and Medical students. Conclusion: The overall findings of this study indicate that the Persian version of the JSPE is a valid and reliable instrument for evaluating physician-patient empathy in Iranian Population
Physician-patient relation,Empathy,Questionnaire,Cross-cultural comparison,Iran
https://jkmu.kmu.ac.ir/article_16537.html
https://jkmu.kmu.ac.ir/article_16537_bb1a9b30eef2a622b1b2ee2cf10f082e.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
19
6
2012
02
01
Challenges in the Treatment of Epilepsy
212
224
EN
H.A
Ebrahimi
Professor of Neurology, Neurology Research Center, Afzalipour School of Medicine, Kerman University of Medical Sciences,
Kerman, Iran
Epilepsy is one of the most important diseases of nervous system that involves 0.5-1% of the population. In spite of new developments in antiepileptic drugs, between 20-30% of epileptic patients can not be controlled and even those who are controlled with antiepileptic drugs suffer from their adverse events. Epileptic patients have different challenges in relation to the disease and its medications. This article has reviewed these challenges. The most common challenges are misdiagnosis of epilepsy or type of epilepsy resulting in wrong drug administration, noncompliance with antiepileptic drugs, adverse effects of antiepileptic drugs, drug resistance, non-pharmacological interventions and their limitations, photosensitivity, epilepsy problems in children and the elderly, pregnancy and epilepsy, driving and epilepsy and finally sudden death in epileptic patients.
Epilepsy,Anticonvulsants,Drug resistance,Adverse effects
https://jkmu.kmu.ac.ir/article_16538.html
https://jkmu.kmu.ac.ir/article_16538_d80f6393a8b2811cd6b6ac53499f5c02.pdf