Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
The Expression of P53 in Leukoplakia, Squamous Cell Carcinoma and Normal Oral Epithelium Using Immunohistochemical Staining Method
1
11
EN
SH
Adhami
Assistant Professor of Oral Pathology, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
F
Baghaei
Assistant Professor of Oral Pathology, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
SH
Kakooi
Assistant Professor of Oral Medicine, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
M
Raoof
Assistant Professor of Endodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran
Background & Aims: Leukoplakia is the most common premalignant or potentially malignant lesion of the oral mucosa and its potentiality for malignant transformation is unpredictable. The aim of the present study was to evaluate p53 expression in normal oral epithelium, leukoplakia and oral squamous cell carcinoma. Methods: The standard Biotin streptavaidin peroxidase immunohistochemical staining method was used to study the expression of p53 on formalin fixed, paraffin embedded blocks of 8 cases of squamous cell carcinoma, 20 cases of leukoplakia and 10 cases of normal oral epithelium. Results: There was no significant difference between immunostaining of leukoplakia and normal epithelium groups in the expression of P53, but the distribution patterns of p53 was mainly localized in the basal layer in the group of normal oral mucosa, while it extended into the suprabasal cell layer in leukoplakia group. P53 expression in squamous cell carcinoma group was higher than other groups. Conclusion: Considering the findings the expression of p53 in suprabasal cell layers in leukoplakia might show poor clinical outcome and alterations of p53 might be an important factor in the development of oral cancer.
Squamous Cell Carcinoma,Oral leukoplakia,neoplasm,immunohistochemistry,Tumor suppressor protein p53
https://jkmu.kmu.ac.ir/article_17309.html
https://jkmu.kmu.ac.ir/article_17309_97eed2a99730e3cf8621f4e49afa0e84.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
Elimination of Copper and Zinc from Industrial Wastes by Mutated Bacteria
13
24
EN
M.R
SHekibaei
Associate Professor, Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran.
A
KHosravan
Instructor, High tech Research Center, Kerman, Iran
A
Farahmand
Research Assistant, High tech Research Center, Kerman, Iran
S
Zare
Ph.D. Candidate, Department of Biology, Shahid Bahonar University, Kerman, Iran
Background & Aims: Today, toxic effluents have created ecological and health problems in and around the industrial cities resulting in death of nearby living organisms. The aim of this research was to increase the elimination of copper and zinc from copper factory effluents in Kerman/Iran through mutation inducing in metal-resistant bacteria by using Acriflavine, Acridine orange and Ethidium bromide. Methods: A total of 20 strains of Pseudomonas spp. were isolated from water and soil of the factory and subjected to microbiological identification. Maximum Inhibitory Concentration (MIC) to Cu and Zn were determined by agar dilution method. Those strains with the highest MIC to these metals (5mM) were subjected to 400-3200mg/L of the above mutagenic agents. After determination of MIC those colonies which were capable to grow on 20mM copper were selected for atomic absorption spectroscopy. Results: According to the atomic absorption spectroscopy of dried biomass obtained from resistant strains after exposure to mutagenic agents, strains 6,7,8,9,10,13 & 16 showed the highest accumulation of CU and Zn (10mM for Cu & 20mM for Zn). Strain 13 had the highest absorption of Cu (0. 35%/mg biomass) and strain 10 showed the highest accumulation of Zn (0.33%/mg biomass). Conclusion: Elimination of heavy metals by artificially mutated bacteria can be suggested as a cost effective solution to this environmental health issue.
Pollution,Industrial Waste,Mutation,P.seudomonas
https://jkmu.kmu.ac.ir/article_17310.html
https://jkmu.kmu.ac.ir/article_17310_6c136b9e4ea2095bafe940eecf5adf95.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
Sonographic and Serological Survey of Hydatid Disease in Rural Regions of Shahdad and Chatroud, Kerman Province, 2006-2007
25
34
EN
S.S
Moazezi
Master of Science in Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
M
Fasihi harandi
Associate Professor, Department of Parasitology and Leishmaniasis Research Center, School of Medicine, Kerman University
of Medical Sciences, Kerman, Iran.
M
Saba
Assistant Professor, Department of Radiology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
H
Kamyabi
Research Assistant, Department of Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
F
SHikhzadeh
Research Assistant, Kerman Health Center, Kerman University of Medical Sciences, Kerman, Iran.
Background & Aims: Human cystic Echinococcosis (CE) caused by larval stage of Echinococcus granulosus, has a cosmopolitan distribution and is particularly more prevalent in rural regions. Iran is an endemic focus for CE and human cases are reported regularly from medical centers in different parts of Iran including Kerman. The objective of this study was to determine the prevalence of hydatidosis among rural communities in Shahdad and Chatroud in Kerman province. Methods: A total of 451 individuals (87 males and 364 females) were selected by randomized cluster sampling between 2006 and 2007. Demographic features were recorded in a questionnaire and abdominal ultrasonography was carried out. Then 5 ml venous blood sample was collected for ELISA test. Data were analyzed using Chi-square test and Multivariate logistic regression for risk factors analysis. Results: Two hydatid cases (0.4%) were detected by ultrasonography. Serological results showed 8.8 % (37 cases) seropositivity, and females (9.7%) were more positive than males (4.9%). There was a significant difference in CE prevalence in different age groups (p=0.012). Results showed the seroprevalence of 8.5% and 9% in Shahdad and Chatroud respectively and there was no significant difference in CE prevalence rates between the two regions. Multivariate logistic regression showed that females were 4.6 times more likely to be infected than males (OR=4.6, 95% CI=1.27-16.79). Proper washing of vegetables decreased the probability of infection by 71% (OR=0.29, 95% CI=0.13-0.65).Dog ownership was not a significant risk factor of CE (OR=0.28, 95% CI=0.06-1.22, P=0.09). Housewives (9.6%) and farmers (9.1%) were more seropositve than other groups. Canclusion: The study showed that seroprevalence of hydatid disease is relatively high in rural regions of Shahdad and Chatroud.
Echinococcosis,hydatidosis,Ultrasound,Seroprevalence,Epidemiology,Kerman
https://jkmu.kmu.ac.ir/article_17311.html
https://jkmu.kmu.ac.ir/article_17311_3e1ced571fb7b31baf1efb90dc06a420.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
Anti-hyperglycemic Activity of Four Plants Extracts Effective against Alpha Glucosidase in Normal and Diabetic Rats
35
44
EN
A
GHolamhoseinian
Associate Professor of Biochemistry, Physiology Research Center and School of Medicine, Kerman University of Medical Sciences, Kerman,
Iran.
H
Falah
Master of Science in Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
F
SHarififar
Associate Professor of Pharmacognosy, School of Pharmacy and Pharmaceutics Research Center, Kerman University of Medical Sciences,
Kerman, Iran
Background & Aims: Postprandial hyperglycemia results in the development of chronic complications. Therefore, its control is critical in the treatment of diabetes. One of the main therapeutic approaches for reducing postprandial hyperglycemia is to reduce the absorption of carbohydrates by enzyme inhibitors such as Acarbose which have already been clinically used as a medicine for diabetic patients. Methods: Aqueous and methanolic extracts of Zataria multiflora, Pistachio hulls, Myrtle leaves and Quercus infectoria which had inhibited alpha glucosidase (>90%) in our previous study were prepared by maceration method. Male wistar rats were divided into two, streptozotocin-induced diabetic group and non-diabetic group. Then each group subdivided into 6 groups of control receiving 2g/kg maltose, positive control receiving 2g/kg maltose as well as Acarbose and 4 other groups receiving 2g/kg maltose and one of the prepared extracts (1000mg/kg) after deprivation of food. Blood glucose level was measured before and 30, 60 and 120 min. after extract administration. Results: In non-diabetic rats, Quercus infectoria and Myrtle extracts significantly reduced the postprandial glucose levels (60 and 50% respectively, P<0.0001) comparing to the control. The effect of Pistachio extract on reduction of blood glucose was not significant. Zataria multiflora extract had no significant effect (P>0.05) but showed a reveres effect on plasma glucose and remained at high level up to 60 minutes. Conclusion: Further studies must be done for proving the efficacy of Zataria multiflora and Quercus infectoria for hyperglycemic patients with uncontrolled diets.
Diabetes Mellitus,Alpha glucosidase,Multiflotriol,Pistachio,Myrtle,Quercus
https://jkmu.kmu.ac.ir/article_17312.html
https://jkmu.kmu.ac.ir/article_17312_5060419e7b165ed2a21eab6e93dde05f.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
Demographic and Clinical Features of Ulcerative Colitis Patients in Kerman City during 2005-2007
45
53
EN
M.J
Zahedi
Associate professor of Internal Medicine, School of Medicine, Physiology Research Center, Kerman University of Medical
Sciences, Kerman, Iran
S
Darvish moghadam
Associate professor of Internal Medicine, School of Medicine, Physiology Research Center, Kerman University of Medical
Sciences, Kerman, Iran
M
Hayatbakhsh abasi
Associate professor of Internal Medicine, School of Medicine, Physiology Research Center, Kerman University of Medical
Sciences, Kerman, Iran
Z
Dalir sani
General Practitioner
Background & Aims: Ulcerative colitis is one of the inflammatory bowel diseases with unknown etiology.
Genetic and environmental factors are thought to be effective in this disease. According to the limited studies
in our country, general assessment of demographic features and clinical symptoms seems to be essential.
Methods: In this prospective and cross-sectional study, 85 cases of ulcerative colitis referred to the 3
gastroenterologist offices in Kerman city were evaluated during 2005-2007. The diagnosis was confirmed
based on clinical features, colonoscopy, pathology and ruling out of other causes. Data were collected
through direct interview and analyzed by SPSS 15 software.
Results: Mean age at the time of diagnosis was 33.3113.12. Duration of symptoms onset until diagnosis
was 8 months. Male to female ratio was 0.8/1 and the most important clinical manifestations were
rectorhagia and bloody diarrhea (92.9%), abdominal pain (20%), mucoid diarrhea (13.1%), weight loss
(11.2%) and fever (4.4%). The pattern of disease was chronic intermittent in 83.5%, chronic continuous in
13% and acute self-limited in 3.5%. Anatomically disease was limited to the rectum (8.3%), recto sigmoid
(23.5%), recto sigmoid and descending colon (28.5%), transverse colon (21.2%) and pan colitis. (18.8%).
Extra intestinal manifestations were seen in 22.35% of cases of whom 12.96% had oral involvement, 5.85%
had hepatic involvement and 3.53% had musculoskeletal involvement.
Conclusion: Recurrent inflammatory bowel disease is one of the important reasons of referral to
gastroenterologists. Ulcerative coilitis in Kerman is similar to other countries of world epidemiologically,
demographically and clinically but its seems that severe clinical courses, extra intestinal manifestations and
second old age peak to be less comparing to other studies.
Ulcerative colitis,signs and symptoms
https://jkmu.kmu.ac.ir/article_17313.html
https://jkmu.kmu.ac.ir/article_17313_eba7a2c22fdd9921a969931e1fdeb0eb.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
The Relationship between Serum Level of Osteoprotegerin and Post-lominectomy Epidural Fibrosis in Rabbit
55
64
EN
I
Lotfinia
Associate Professor of Neurosurgery, Tabriz University of Medical Sciences, Tabriz, Iran
E
KHalagi
Resident of Neurosurgery, Tabriz University of Medical Sciences, Tabriz, Iran
M
Mesgari
Research Assistant, Tabriz University of Medical Sciences, Tabriz, Iran
R
Estakhri
Assistant Professor of pathology, Tabriz University of Medical Sciences, Tabriz, Iran
Background & Aims: Epidural fibrosis (EF) is part of natural physiological tissue response to laminctomy and it may be an important cause of failed back surgery syndrome (FBSS). There is still no effective medical or surgical therapy for EF. Osteoprotegerin (OPG) is a soluble member of the tumor necrosis factor receptor family that might play a role in postoperative EF. The objective of this study was to evaluate the relationship between serum OPG level and EF after laminectomy. Methods: Sixty rabbits were employed and divided randomly into two groups. Group A contained 20 rabbits as controls and group B contained 40 rabbits that underwent bilateral laminecyomy at L4 and L5 lumbar levels under an approved surgical protocol. Serum sample was obtained after 8 weeks from all rabbits and then the animals in group B were killed in accordance with the guidelines for euthanasia. The spine segment from L3 to L6 was removed en bloc. The amount of scar tissue at the laminectomy defect was scored on an ordinal score from 0 to 3 with a scoring system similar to that of He et al. According to the amount of scar the rabbits in group B, were divided into two groups: B1 with low fibrosis (grade 0, 1) and B2 with high fibrosis (grade 2, 3). The serum OPG level of groups A, B1 and B2 were determined using ELISA method. The one way ANOVA was used to assess significant difference among groups. Results: Serum OPG level ranged from 0-4 Pmol/l with a median of 1.5 Pmol/l in group A, 0-6 Pmol/l with a median of 2.42 Pmol/l in group B1 and 0-6 Pmol/l with a median of 2.15 Pmol/l in group B2. There was not any significant difference in OPG levels among these 3 groups. Conclusion: This study showed no relation between OPG level and existence of postlaminectomy EF after 8 weeks
Osteoprotegerin,fibrosis,Epidural space,Laminectomy
https://jkmu.kmu.ac.ir/article_17314.html
https://jkmu.kmu.ac.ir/article_17314_07588256899e0d76fcd58b1959d63484.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
Risk Factors of Nasal Carriage of Methicillin-Resistant Staphylococcus Aureus and its Antibiotic Susceptibility Pattern in Namazi hospital Healthcare Workers in Shiraz, Iran
65
77
EN
M
Askarian
Professor of Community Medicine, School of Medicine, Shairaz University of Medical Sciences, Shiraz, Iran
A.H
Zeinalzadeh
Resident of Community Medicine, School of Medicine, Shairaz University of Medical Sciences, Shiraz, Iran
A
Japoni
Assistant Professor of Molecular Bacteriology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
A.V
Alborzi
Professor of Pediatrics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Background & Aims: Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. The aim of this study was to determine the risk factors of nasal carriage of MRSA and its antibiotic susceptibility pattern among healthcare workers at Namazi Hospital (Shiraz-Iran) Methods: In a cross-sectional study from July to November 2006, nasal swabs were taken from 600 stratified randomly selected health care workers. The isolates were identified as S. aureus based on morphology, gram stains, catalase test, coauglase test and DNase Agar. To differentiate Methicillinsusceptible S. aureus (MSSA) and Methicillin Resistant S. aureus (MRSA), agar screen plate was used. All methicillin-resistant isolates were examined for mecA genes existence by PCR performance. The sensitivity patterns of S.aureus isolates were determined by disc diffusion and E-test method. Results: Nasal screening identified 186 (31%) S. aureus carriers of whom, 154 ones (82.8%) were MSSA and 32 ones (17.2%) were MRSA. There was no significant association between related risk factors and gender, age, years of healthcare service and level of education. In the univariate analysis, a statistically significant difference was found only based on occupation (P=0.032) between carriers of MSSA and MRSA. In multivariate analysis(logistic regression), having nursing occupation (p=0.012, OR=3.6, 95%CI=1.3-9.7) was independently associated with MRSA carriage. All of the MRSA strains were sensitive to mupirocin. Conclusion: This study revealed that having nursing occupation is independently associated with MRSA carriage since all S.aureus isolates were susceptible to mupirocin, topical mupirocin could be used successfully to eradicate nasal staphylococcal colonization and carriers.
Staphylococcus aureus,Methicillin,risk factors,Carrier,Microbial sensitivity,Health care providers
https://jkmu.kmu.ac.ir/article_17315.html
https://jkmu.kmu.ac.ir/article_17315_3ed19e7680f839ae9edcd83d63bcb245.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
Composition and Antioxidant Activity of the Essential Oil and Methanolic Extract of Ziziphora Clinopodioides Lam in Preflowering Stage
79
86
EN
H
Amiri
Assistant Professor, Biology Department, Lorestan University, Khoramabad, Iran
Background & Aims: Ziziphora clinopodioides Lam belongs to the Labiatae family and its dried aerial parts are used as flavors and also in the treatment of cold and cough. Methods: The chemical composition of the essential oil obtained from aerial parts by hydro distillation method was analyzed by GC and GC/MS. The methanolic extract was also obtained from the aerial parts of the plant. Samples were subjected to a screening for their possible antioxidant activities by using 2, 2- diphenyl-1-picrylhydrazyl (DPPH) and -carotene-linoleic acid. Results: Twenty three constituents accounting to 96.6% of the total oil were identified. Major components of the oil were pulegon (30.1%), thymol (21.3%), p-mentha-3-en-8-ol (12.9%), piperitenone (9.3%) and 1, 8-cineol (4.1%), respectively. In the DPPH test system, the IC50 value for antioxidant activity of methanolic extract was determined to be 28.4µg/ml, whereas it was 55.3 µg/ml for the oil. In -carotene-linoleic acid test system, antioxidant activities of the extract and oil were 89.3% and 61.6%, respectively. Conclusion: In general, methanolic extract of ziziphora clinopodioides lam showed greater antioxidant activity than its essential oil.
Ziziphora Clinopodioides,Essential oil,Antioxidant activity,Methanolic extract
https://jkmu.kmu.ac.ir/article_17316.html
https://jkmu.kmu.ac.ir/article_17316_62f0bd8d71005d174a886b0d320b5a4b.pdf
Kerman University of Medical Sciences
Journal of Kerman University of Medical Sciences
1023-9510
2008-2843
15
1
2009
12
01
A 3-Year old Child with Gastric Outlet Obstruction due to Pyloric Web: A Case Report
87
91
EN
M
Baghai
Associate Professor of surgery, Kerman University of Medical Sciences, Kerman, Iran
F
Gazanfaripour
Assistant Professor of Pediatrics, School of Medicine & Physiology Research Center, Kerman University of Medical Sciences,
Kerman, Iran
M
Ahmadipour
Resident of Pediatrics, Kerman University of Medical Sciences, Kerman, Iran
The most common cause of gastric outlet obstruction in neonates and infants is hypertrophic pyloric stenosis. Pyloric web as a cause of gastric outlet obstruction is relatively rare in children and presents with non bilious vomiting, abdominal pain and failure to thrive. The onset of symptoms is usually late with nonspecific symptoms like vomiting and failure to thrive that seems to be due to neuromuscular incoordination. Here we report a 3 year-old boy with prolonged fever, anorexia, and intractable postprandial non bilious vomiting and weight loss since 20 days prior to admission. Barium meal study revealed gastric outlet obstruction with a dilated stomach. The patient underwent laparotomy and pyloric web was confirmed as the cause of the symptoms and Heineke-Mikulicz pyloroplasty led to disappearing of the entire patient’s problems.
Pyloric web,Gastric outlet,Obstruction
https://jkmu.kmu.ac.ir/article_17317.html
https://jkmu.kmu.ac.ir/article_17317_a9bacb3e2e2d160ad2a5ee11220465ee.pdf