Kerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Supporting the in vitro Expansion of Human Cumulus Cells as an Initial Step for Culturing the Ovarian Follicles and Assembling an Artificial Ovary3133329200710.22062/jkmu.2022.92007ENMojganMoshrefiMedical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran & Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Science, Yazd, Iran0000-0002-6306-7881AbbasAflatoonianResearch and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Science, Yazd, Iran0000-0002-2896-6136SaeedGhasemi-EsmailabadMedical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran & Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran & Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran0000-0002-9652-8699MojganKarimi-ZarchiEndometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran & Department of Gynecology and Oncology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran0000-0002-8734-9971FatemehSadeghian-NodoushanMedical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran & Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran0000-0003-2767-7882SajadShahmohammadiMedical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran & Department of Biology, Science and Arts University, Yazd, IranHabibNikukarMedical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran & Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran0000-0001-9225-6019Journal Article20210628<strong>Background:</strong> Assembling an artificial ovary needs supporting the in vitro growth of cumulus cells, and finally, follicles. This study aimed to determine the appropriate cocktail for culture of cumulus cells (CCs).<br /><strong>Methods:</strong> CCs were collected from healthy women and cultured with 9 cocktails of basal media, supplemented with 10% and 20% fetal bovine serum (FBS) and 1% and 2% human serum albumin (HSA). Ovarian cells were isolated from cortex, medulla, and hilum, and their conditioned media (CM) were collected. Expression of GDF9 in ovarian cells was evaluated. CCs were treated with various concentrations of CMs from ovarian cells and mesenchymal stem cells. Also, they were cultured with various concentrations of supplements including L-Glutamine, bovine serum albumin (BSA), HSA, insulin transferrin selenium (ITS), Follitropin alfa, and Pregnyl. Also, they were treated with various concentrations of follicular fluids (FFs), collected from patients with different infertility etiologies. Finally, CCs proliferation and culture stability were evaluated.<br /><strong>Results:</strong> All the ovarian cells expressed GDF9. DMEMF12 + 20% FBS was the most suitable cocktail for CCs. 20% FBS was superior to 10% FBS. HSA alone could not support the growth of CCs. The CMs of (cortical + hilar + medullar) cells and FFs from healthy women caused higher CCs proliferation. 17 mM/l L-Glutamine, 24 mg/ml BSA, 20 mg/ml HSA, 10 ng/ml ITS, 300 mIU/ml Follitropinα, and 3.5 IU/ml Pregnyl led to higher CCs proliferation.<br /><strong>Conclusion:</strong> Supplementation of the basal medium with CMs, serums, FFs, hormones, ITS and L-Glutamine, can better support the culture of CCs.https://jkmu.kmu.ac.ir/article_92007_f1f72ac27726fba61b2e43f354b6457b.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Using EMA and Calretinin on Cell Blocks for the Differentiation of Reactive Mesothelial Cells from Adenocarcinoma Cells in Pleural Effusions3333409200810.22062/jkmu.2022.92008ENMassoodHosseinzadehDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran0000-0003-0531-0457NedaSoleimaniDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran0000-0003-0803-9818MaryamMohammadnia AvvalDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran0000-0002-8276-4121PerikalaVijayananda KumarDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran0000-0001-8315-7000NavidOmidifarDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran0000-0003-3391-926XMansourehShokripourDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran0000-0002-8339-322XSahandMohammadzadehDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran0000-0001-6974-1323Journal Article20211210<strong>Background:</strong> Effusion in body cavities can be considered as the presentation of malignancy or inflammatory conditions. Pleural fluid cytology is a popular diagnostic tool for the differentiation of adenocarcinoma cells (AC) from reactive mesothelial cells (RMC). However, there are many sources of controversies and errors in this technique that should be addressed.<br /><strong>Methods</strong>: This case-control study aimed to evaluate the use of immunohistochemistry markers, namely epithelial membrane antigen (EMA) and calretinin, on cell blocks to differentiate between RMC and AC in pleural effusions. Suspected malignant effusions were selected according to the clinical data and their equivocal cytological smears. A total of 80 samples corresponding to the fresh specimens sent from the Department of Internal Medicine to the Cytology Laboratory of Faghihi Hospital during Jan 2017-Feb 2018 comprised the case group. In addition, the control group entailed 80 non-malignant pleural samples with RMC.<br /><strong>Results</strong>: We observed that 74 (out of 80) effusion samples were strongly positive for EMA (92.5%). The sensitivity, specificity, and efficiency of the EMA marker were 92.5%, 95%, and 93.7%, respectively. The results of the calretinin assessment indicated 78 (out of 80) positive cases in the control group (97.5%). The sensitivity, specificity, and efficiency of calretinin staining were 97.5%, 98.7%, and 98.1%, respectively.<br /><strong>Conclusion</strong>: According to the results of the current study, EMA and calretinin are two reliable markers with acceptable accuracy in differentiating between RMC and AC.https://jkmu.kmu.ac.ir/article_92008_4df2a480232a3d56ed7b009958f5f0f6.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Dose-dependent Effect of β-caryophyllene on Glycemic Control of High-Fat Diet and Fructose-Induced Type-2 Diabetic Rats3413479200910.22062/jkmu.2022.92009ENVadivelManiDepartment of Biochemistry, Arunai Medical College and Hospital, Tiruvanamalai-606603, Tamilnadu, India0000-0002-7592-7666AnandhiDanavelDepartment of Biochemistry, Meenakshi Ammal Dental College and Hospital, Chennai-600095, Tamilnadu, India0000-0002-2863-6671ManikandanBalrajDepartment of Physiology, Konaseema Institute of Medical Sciences and Research Foundation, Amalapuram, East Godavari Dt-533201, Andhra Pradesh, India0000-0001-6028-1260GayathriVenkatasanDepartment of Physiology, Krishnadevaraya College of Dental Science and Hospital, Bangalore, Karnadaka, India0000-0002-0416-1499MegalathaLibinDepartment of Biochemistry, Zydus Medical College and Hospital, Dahod-389151, Gujarat, India0000-0002-9034-8881Journal Article20211218<strong>Background:</strong> Among many human diet-related disorders, Diabetes mellitus (DM) stands on the top of the table, its persistent and perdurable threat/stress response to systemic functions and endocrine control makes it's more popular. The management of diabetes and related chronic diseases has focused on the use of indigenous natural compounds, derived from plant sources that possess medicinal properties.<br /><strong>Methods: </strong>Wistar albino rats were fed with high-fat diet comprising 2% cholesterol, 1% cholic acid, 30% coconut oil, 67 % percent regular rat feed, and 25% fructose through drinking water for 60 days to induce type-2diabetic. After induction, type-2 diabetic rats were treated with β-Carophyllene (50, 100, 200, and 400mg/kg body weight once a day, orally) for 30 days, respectively. Fasting Blood glucose, liver and kidney function markers were analyzed.<br /><strong>Results:</strong> Diabetic animals showed elevated blood glucose level when compared to control. Treatment with 50 and 100 mg/kg b.wt β-Carophyllene did not reach control level. Whereas, 200 and 400 mg/kg b.wt doses effectively reduced the blood glucose levels in diabetic animals.<br /><strong>Conclusion:</strong> Liver function markers such as ALT, AST, and ALP and kidney function markers like urea and creatinine were also found to be elevated in diabetic animals. β-Carophyllene effectively reduced it. No toxicity was found in 200 and 400 mg/kg b.wt β-Carophyllene treated animals. Since blood glucose was restored to normal range at 200 mg dose itself, the same dose was selected as optimal dose for further study to elucidate the anti-diabetic potential.https://jkmu.kmu.ac.ir/article_92009_d4820edd2a7d6cc24b5a8a0b0956cc62.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701The Relationship between Opium Addiction and the Severity of Atherosclerosis, Lipid Profile, Inflammatory Cytokines, and Renal Function in Non-diabetic Patients Subjected to Angiography3483579201010.22062/jkmu.2022.92010ENParinazOnikzehCardiovascular Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran0000-0002-2587-0198AidaKazemiClinical Research Development Unit, Shafa Hospital, kerman University of Medical Sciences, Kerman, IranFatemehRahmaniDepartment of Cardiology, School of Medicine, Bam University of Medical Sciences, Bam, IranMohamadMasoomiCardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, IranMinaMoridiCardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran0000-0001-9936-8339Journal Article20220112<strong>Background: </strong>Despite traditional beliefs about the beneficial effects of opium use, current evidence suggests an adverse effect of this substance on cardiovascular disease (CVD) risk factors. The present study aimed to investigate the association between opium-addiction status and lipid profile, the severity of atherosclerosis (measured by Gensini score), inflammatory cytokines, and renal function among non-diabetic patients subjected to angiography.<br /><strong>Methods: </strong>This cross-sectional study was conducted on 95 post-angiography patients. Routine tests including lipid profile, blood urea, creatinine, and hematocrit were collected at the time of admission. Also, serum levels of IL-6 and IL-8 were measured using ELISA. Independent sample t-test, Mann-Whitney U test, and chi-square were used to compare variables according to opium addiction. Multivariate regression was conducted to adjust the effect of potential confounding variables.<br /><strong>Results: </strong>Opium-addict subjects had a higher IL-6 (P=0.049) level and PLT number (P=0.005). In contrast, there was a lower level of TG (P=0.015) and GFR (P=0.039) in the opium-addict group. There was no association between opium addiction and other variables (P>0.05).<br /><strong>Conclusion: </strong>Although no significant association was observed between addiction and atherosclerosis and even there was a lower level of TG in addict subjects, there was a direct association between the serum IL-6 levels (an important inflammatory cytokine with adverse effects on coronary artery disease) and opium addiction. It has been revealed that confounding variables affect the relationship between opium use and CVD outcomes. Therefore, well-designed prospective studies controlling a vast range of general variables seem to be necessary.https://jkmu.kmu.ac.ir/article_92010_d8adb8b353cc7ec72ad6767f45e54643.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701The Effect of Crocin and Losartan on Biochemical Parameters and Oxidative Stress Markers in Diabetic Nephropathy Rats Model3583679201110.22062/jkmu.2022.92011ENYaserMohammadiQaen School of Nursing and Midwifery, Birjand University of Medical Sciences, Birjand, Iran
& Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran0000-0003-1819-853XFatemehSalmaniDepartments of Epidemiology and Biostatistics, School of Health Social Determinants of Health Research Center, Birjand University of Medical Sciences, Birjand, Iran0000000303708429MohammadZangooeiDepartment of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran0000-0003-3733-4410AzamRezaei FarimaniDepartment of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran & Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran0000-0002-7853-2993Journal Article20210127<strong>Background: </strong>Diabetic nephropathy (DN) is the most common cause of the end-stage renal disease (ESRD) globally. This study aimed to evaluate the effect of Crocin and Losartan on DN in diabetic rats.<br /><strong>Methods:</strong> A single dose of Streptozotocin (50 mg/kg IP) was administered to 40 male Wistar rats to induce diabetes. Crocin and Losartan (50 and 25 mg/kg, respectively) were given orally for four weeks. The study groups were untreated control, Diabetes, Crocin, Losartan, and Losartan-Crocin.<em> </em>At the end of the intervention, blood was tested for FBS, urea, UA, Cr, albumin, TG, TC, LDL-C, HDL-C, TAC, and MDA.<br /><strong>Results:</strong> Serum levels of FBS, urea, UA, TG, TC, and LDL-C increased significantly in the diabetic group compared to the untreated control group (P = 0.001), while albumin and HDL-C decreased significantly (P = 0.001). In the Crocin group, serum FBS, urea, TG, TC, and LDL-C levels were significantly lower than the diabetic group (P = 0.001), while serum albumin levels were significantly higher (P = 0.02). Serum levels of TAC and MDA in the Losartan group increased (P=0.04) and decreased significantly (P = 0.001) compared to the diabetic group, respectively.<br /><strong>Conclusion:</strong> The findings of the present study showed that Crocin could control hyperglycemia and prevent DN progression. It appears that combining Losartan with sufficient doses of Crocin improves its efficacy. However, understanding the exact mechanism of these changes requires further studies.<em> </em>https://jkmu.kmu.ac.ir/article_92011_7a6de2f3fcb165854c4a590b2ea134b7.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Protein E-Peptide Driven Vaccine for Novel Coronavirus: Immunoinformatics3683779201210.22062/jkmu.2022.92012ENSaraIranparastStudent Research Committee, Ahvaz J undishapur University of Medical Sciences, Ahvaz, Iran
& Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran0000-0002-5134-487XMehriGhafourianDepartment of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran0000-0003-0140-7083MaryamTahmasebi BirganiDepartment of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
& Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran0000-0002-9624-1903Journal Article20210823<strong>Background:</strong> The COVID-19 pandemic is a red alarm for global health, so researchers around the world are working on it to design an effective vaccine against it. Protein is one of the candidates for vaccine development which plays an important role in virus pathogenesis. Accordingly, this study was done to evaluate the critical characteristic of this protein as a vaccine candidate using in-silico analysis.<br /><strong>Methods:</strong> The sequence of SARS-CoV-2-associated E protein was recruited from NCBI and subjected to the IEDB software to evaluate the most potent epitopes. The capacity of the interactions of HLA-I and HLA-II molecules with selective peptides was studied using IEBD tool kit. The E protein sequence was subjected to B cell and T cell tests to realize the most promising peptides that could act as COVID-19 vaccine.<br /><strong>Results:</strong> Among the tested peptides for the T cell-test, this study found two interesting epitopes: VSEETGTLI and LTALRLCAY that exhibit high binding affinity as a strong indicator to HLA-I and HLA-II alleles together. The results of the analysis demonstrated that some epitopes in the E protein have a relatively higher immunogenicity score based on interaction with HLA-II, such as SEETGTLIVNSVLLF, TLIVNSVLLFLAFVV, LAFVVFLLVTLAILT, LAILTALRLCAYCCN, and SVLLFLAFVVFLLVT. Furthermore, two sequences (FVSEET and PSFYVYSRVKNLNSSRVP) were reported as the selective linear epitopes for B cell, on the surface of SARS-CoV-2 E protein and being Immunogenic.<br /><strong>Conclusion: </strong>Since E protein can stimulate favorable immune responses, T and B- cell responses, its evaluation in patients with COVID-19 is of a great importance.https://jkmu.kmu.ac.ir/article_92012_27a3cbedd39765356787d359c9aef8d5.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Evaluation of Plasma Level of Heme-Oxygenase-1 in Neonatal Hypoxic Ischemic Encephalopathy3783849201310.22062/jkmu.2022.92013ENShahinMafinezhadDepartment of Pediatrics, North Khorasan University of Medical Sciences, Bojnurd, Iran0000-0003-3600-3943GhasemBayaniDepartment of Pediatrics, North Khorasan University of Medical Sciences, Bojnurd, Iran0000-0001-8395-7470HojatolahEhteshammaneshDepartment of Pediatrics, North Khorasan University of Medical Sciences, Bojnurd, Iran0000-0002-9751-5224Ananth KumarKammalaDepartment of Obstetrics and Gynecology, School of Medicine, University of Texas Medical Branch, Texas, USA0000-0001-5300-2083HasanNamdar AhmadabadDepartment of Pathobiology and Laboratory Sciences, North Khorasan University of Medical Sciences, Bojnurd, IranJournal Article20210930<strong>Background:</strong><strong> </strong>Neonatal hypoxic-ischemic encephalopathy (HIE) is one of the most common causes of long-term neurological disabilities among children. Various types of cellular stress stimuli, including oxidative stress, inflammation, and hypoxia, induce heme oxygenase-1 (HO-1) enzyme for different kinds of tissues. The purpose of this study was to evaluate the plasma level of HO-1 enzyme in neonatal HIE patients and to determine the relationship between HO-1 enzyme level and clinical severity of HIE.<br /><strong>Methods: </strong>In this case-control study, the plasma level of HO-1 enzyme was measured through sandwich ELISA in 28 newborns with a proven diagnosis of HIE and 31 healthy full-term newborns admitted to Bentolhoda Hospital, Bojnourd, Iran. Newborns with HIE were classified according to the Sarnat staging to mild, moderate, and severe HIE. Maternal and neonatal data were recorded in checklists and compared between the two groups.<br /><strong>Results: </strong>The mean plasma level of HO-1 enzyme in HIE patients <em>was significantly higher than that in the control group </em>(104.0 ± 4.01 and 91.63± 2.67 pg/ml, respectively, P=0.011<em>). We also found that </em>plasma HO-1 levels were significantly higher in severe neonatal HIE patients compared to mild and moderate neonatal HIE patients (121.0 ± 8.48Vs. 91.23 ± 3.35 and 105.5 ± 5.76, <br /><em>P</em> ˂ 0.001)<em>. </em><br /><strong>Conclusion: </strong>Our findings suggested that HO-1enzyme may be associated with the pathophysiology and clinical severity of neonatal HIE. We suggest further research on the correlation of plasma level of HO-1 enzyme at birth with the multi-organ dysfunction and abnormal neurodevelopmental outcomes in full-term newborns with HIE.https://jkmu.kmu.ac.ir/article_92013_59642f4d629be6571bc3546f6338b887.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Resiliency and its Relationship with Secondary Traumatic Stress among Nursing Staff during COVID-19 Pandemic3853949201410.22062/jkmu.2022.92014ENMahdiAbdolkarimiDepartment of Health Education and Health Promotion, School of Health, Rafsanjan University of Medical Sciences, Rafsanjan, Iran0000-0002-2781-2821SeyedhamidSeyedbagheriDepartment of Pediatric Nursing, School of Nursing and Midwifery, Geriatric Care Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran0000-0002-2591-9131HamidGanjehAli-Ibn Abi-Talib Hospital, Rafsanjan University of Medical Sciences, Rafsanjan, Iran0000-0003-3635-9106MahmoodKahnoojiDepartment of Internal Medicine, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran0000-0002-8851-8917Mohammad AliZakeriSocial Determinants of Health Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran & Non-Communicable Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran0000-0000-1500-391XJournal Article20211018<strong>Background</strong><strong>: </strong>Nurses are front-line health-care workers for patients with severe Coronavirus disease (COVID-19) symptoms. The aim of this study was to determine the resiliency of nurses and its relationship with secondary traumatic stress (STS) in pandemic conditions.<br /><strong>Methods: </strong>This cross-sectional study was performed on 233 nurses working in a medical center providing services to patients with COVID-19 in southeast Iran from May to August 2020. Data were collected using demographic questionnaire, Connor-Davidson Resilience Scale (CD-RISC) and The Secondary Traumatic Stress Scale (STSS). Pearson correlation coefficient was used to determine the relationship between variables and data were analyzed through SPSS22.<br /><strong>Results:</strong> The mean STS score of nurses was 40.82 ± 11.7. The results showed a significant relationship between STS score and Job satisfaction. The mean score of resilience was 60.91± 17.1 in nurses. The resiliency score showed significant difference based on work experience and exposure to COVID-19 disease. The results of correlation test showed that the overall stress score was significantly related to all aspects of resilience except trust (p < 0.05). Also, based on the multiple regression model, positive acceptance (one of the dimensions of resilience) and exposure to COVID-19 disease were the predictors of STS. <br /><strong>Conclusion:</strong> The results showed that there is a significant and inverse relationship between resiliency score and STS. Therefore, according to the present conditions, it is recommended to design programs to improve resilience and reduce stress of nurses during COVID-19 pandemic.https://jkmu.kmu.ac.ir/article_92014_d16fd1949e302e1d1e0f65c3e67a00cc.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Maxillofacial 3D Imaging in Cleidocranial Dysplasia: A Case Report and Literature Review3954009201510.22062/jkmu.2022.92015ENMohamed FaizalAsanDepartment of Oral Medicine and Radiology, A B Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte deemed to be University, Mangalore, India0000-0001-9747-1914SupriyaBhatDepartment of Oral Medicine and Radiology, A B Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte deemed to be University, Mangalore, India0000-0002-4380-0214AnanyaMadiyalDepartment of Oral Medicine and Radiology, A B Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte deemed to be University, Mangalore, India0000-0002-0907-3244Renita LorinaCastelinoDepartment of Oral Medicine and Radiology, A B Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte deemed to be University, Mangalore, India0000-0002-8696-549XG SubhasBabuDepartment of Oral Medicine and Radiology, A B Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte deemed to be University, Mangalore, India0000-0001-9383-7886Journal Article20211017Cleidocranial dysplasia (CCD) is an inherited autosomal dominant disorder affecting the skeletal and craniofacial structures, exhibiting distinct maxillofacial abnormalities. A case of CCD in an adolescent girl who presented with significant maxillofacial abnormalities is reported in this study. She had a total of 58 teeth including the retained deciduous, impacted, and unerupted teeth. The role of radiography in the diagnosis and treatment planning of CCD is inevitable. In our case, the application of cone-beam computed tomography aided in effective treatment planning which included surgical and orthodontic interventions for the management of the maxillofacial abnormalities. Gene testing was done for the patient and the diagnosis of CCD was confirmed. A dental surgeon can be the first person to diagnose the disease because of its striking craniofacial features and associated dental problems. Hence, adequate knowledge about the maxillofacial abnormalities of CCD among dental and maxillofacial surgeons is necessary to aid in an early diagnosis and better aesthetic outcome.https://jkmu.kmu.ac.ir/article_92015_8bc2670f383ae175ca5b77b785d0368f.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Central and Peripheral Thermoregulatory Responses to Cold Exposure: Involvement of Sympathetic System, Nitric Oxide, and Orexin4014109201610.22062/jkmu.2022.92016ENZahraRezaeiDepartment of Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran0000-0001-6506-2286SohrabHajizadehDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran0000-0001-7920-6949Journal Article20220120In hypothermia, the core temperature of the body decreases below 35°C. In this situation, the body initiates some thermal regulatory process. Thermal regulation is the balance between heat production (thermogenesis) and heat loss (thermolysis) during thermal changes. Thermoregulation in skin blood flow can maintain body temperature and so homeostasis. A large body of literature has shown that in cold exposure, the hypothalamus contributes to thermoregulation by affecting skin blood flow. Moreover, some peripheral factors contribute to thermoregulation through modification of skin blood flow. Furthermore, the sympathetic nervous system can regulate the body temperature through a noradrenergic vasoconstrictor and a vasodilator system. As orexin receptors are also found in several peripheral mammal tissues, the activation of the orexin may stimulate the autonomic nervous system to increase blood pressure leading to control of heat balance. The present study aimed to evaluate the activity level and involvement of thermal regulators in cold stress. Generally, more experiments should be accomplished to find the regulatory pathways in these situations. Furthermore, this study was focused on the effect of orexin on thermoregulatory functions. This brief review intended to report the studies revealing the prime effects of orexin on the body temperature through influences exerted on the sympathetic nervous system.https://jkmu.kmu.ac.ir/article_92016_a74f792b270eb235b3bd5003e7c7ff9d.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Intraocular Pressure Changes Two-Six Hours, One Day, and One Week after Phacoemulsification Cataract Surgery and Implantation of Intraocular Lens4114179201710.22062/jkmu.2022.92017ENAliSharifiDepartment of Ophthalmology, Shafa Hospital, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran0000-0003-0713-088XVaresTahmooresiSchool of Medicine, Kerman University of Medical Sciences, Kerman, IranMohammadSharifiSchool of Medicine, Tehran University of Medical Sciences, Tehran, Iran0000-0002-9044-3272NaserNasiriHIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran0000-0002-1505-0866HamidSharifiHIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran0000-0002-9008-7618Journal Article20220212<strong>Background</strong><strong>: </strong>This study was conducted to evaluate the status of eye and intraocular pressure (IOP), two-six hours, one day, and one week after cataract surgery and implantation of the intraocular lens.<br /><strong>Methods:</strong> In a longitudinal study, 159 patients with senile cataracts were evaluated. Phacoemulsification surgery and intraocular lens (IOL) implantation in the capsular bag was done. The examinations including visual acuity measurement, slit-lamp examination, and measurement of IOP were done preoperatively, and three times after the surgery.<br /><strong>Results:</strong> The mean±standard deviation (range) age of the patients was 65.8 ± 10.5 (40-90) years. Preoperatively the IOP was 12.8 ± 3.5 mmHg, it increased to 20.8 ± 10.7, 2-6 hours after surgery (P < 0.0001), and then reached 13.7 ± 4.4 mmHg on the first postoperative day (P < 0.0001), then it decreased to 12.4 ± 2.9 mmHg one week after the surgery (P=0.02). The IOP was 28.6 ± 12.2 mmHg in eyes with epithelial edema which was significantly higher than those with stromal edema (17.3 ± 7.5 mmHg), and those without edema (16.5 ± 7.1 mmHg) 2-6 hours after surgery.<br /><strong>Conclusion:</strong> Since the IOP spike can damage the structure and function of the eye and cause pain, discomfort, and corneal edema that in turn prolongs recovery of vision, especially in those previously damaged, early postoperative examination on the same day after cataract surgery is strongly advised. IOP spikes can be managed safely by aqueous paracentesis without oral, parenteral, or topical drugs. Elevated IOP is more prevalent in eyes with postoperative epithelial corneal edema and in individuals whose cornea is totally edematous.https://jkmu.kmu.ac.ir/article_92017_3af1d9b9e30936084074f084ccaf947c.pdfKerman University of Medical SciencesJournal of Kerman University of Medical Sciences1023-951029420220701Geographical Distribution and Underlying Diseases of Patients with COVID-19 Referred to Afzalipour Hospital in Kerman city4184279201810.22062/jkmu.2022.92018ENMohammad MehdiGhaemiMedical Informatics Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran0000-0001-6427-2740Hamid RezaSamzadehDepartment of Health Information Sciences, Faculty of Management and Medical Information Sciences, Kerman University of Medical Sciences, Kerman, IranSadriehHajesmaeel-GohariMedical Informatics Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran0000-0001-5212-6148ElahehShafieiDepartment of Health Information Sciences, Faculty of Management and Medical Information Sciences, Kerman University of Medical Sciences, Kerman, Iran0000-0002-7746-9355RoghayehErshadSarabiMedical Informatics Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran0000-0001-8926-0156Journal Article20220118<strong>Background: </strong>COVID-19 is a novel disease that infected many people around the world. It is essential to find the potential high-risk locations and provide suitable healthcare interventions to control COVID-19 distribution. Using a geographic information system, this study aimed to investigate the distribution trends of patients with COVID-19 in Kerman, Iran.<br /><strong>Methods:</strong> The information of registered patients of Afzalipour hospital in Kerman city who were admitted before July 22, 2020, was collected and used in this paper. The patients’ addresses were converted into geocodes. The trend of disease prevalence in connection with population density in different parts of Kerman was investigated. After that, the average nearest neighbor analysis was performed to check the random distribution of disease cases. Data were checked for randomness by High/Low clustering analysis.<br /><strong>Results: : </strong>The spread of the COVID-19 disease started in Kerman city’s north, south, and west and then distributed to the center. The southern and western regions were in high-high clusters, and the central and northern regions were in low-low clusters in terms of COVID-19 outbreak risk. Regression showed a significant correlation between underlying diseases and patients' age with the incidence of Covid-19 disease.<br /><strong>Conclusion: </strong>The prevalence of COVID-19 had been higher in densely populated areas and also in areas with poorer economic conditions. Therefore, paying attention to these areas as well as applying strict rules can help control the spread of COVID-19. The result of this study could be useful for public health experts and healthcare managers to manage better this pandemic.https://jkmu.kmu.ac.ir/article_92018_4dba69a05ab77ba3d53bf8597af76a23.pdf