Journal of Kerman University of Medical Sciences

Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective Fraction

Authors

M.H Moshefi 1
M Mehrabani 2
M Moeini 3
F Saffari 4

1 Professor, Department of Pharmaceutics, School of Pharmacy, Kerman University of Medial Sciences, Kerman, Iran

2 Associate Professor, Department of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

3 Pharmacist

4 Ph.D. Candidate of Medical Bacteriology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract
Background & Aims: Today, the emergence of antimicrobial resistance against conventional antibiotics is increasing. Many researchers consider plants with antimicrobial properties as a good alternative. In this study, the antimicrobial activity of Ajuga chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. on 8 bacterial strains wasinvestigated. Methods: The leaf extract was prepared by methanolic maceration. The concentrated, dried extract was fractionated by different solvents including petroleum ether, dichloro methane, and ethyl acetate. Determination of minimum inhibitory concentration (MIC) of crude extract was performed using agar dilution method. Disk diffusion method was used for antimicrobial assay of different fractions. Then, the chemical constituents of the most effective fraction were separated on thin-layer chromatography (TLC) plates. Then, the prepared chromatograms were analyzed using immersion bioautography. Results: According to disk diffusion method, ethyl acetate fraction had stronger antibacterial activity against all tested bacteria. Bioautography of thisfraction showed antibacterial activity against Staphylococcus aureus in Rf = 0.9. However, all tested bacteria had inhibition spots in the site of fraction loading. Conclusion: Antibacterial activity of ethyl acetate fraction will appear if all components are used together.

Keywords

Ajuga chamaecistus
Antibacterial Activity
Immersion bioautography
Journal of Kerman University of Medical Sciences
Volume 21, Issue 2
2014
Pages 313-320

Home

Editorial Board

Guide for Authors

Submit Manuscript

Guide for Reviewers

Contact Us