Correlation between fatty acids intake, serum lipid and lipoproteins with serum creatinine and kidney function in patients with renal failure has been suggested by other investigators. This relationship has not been reported for all types of fatty acids. This study was conducted to determine the association of the consumption of different type of fatty acids, serum lipids and lipoproteins with serum creatinine and glumerular filtration rate(GFR) in healthy adult subjects residing in district 13 of Tehran. There were 15005 subjects participated in TLGS. 1470 persons were randomly selected for nutritional assessment. After excluding under- and over-reporter 486 subjects aged over 20 years remained in the study. Dietary data were collected by two 24-hour dietary recalls. Serum creatinine, total cholesterol, triglyceride and HDL were measured by Selectra 2 autoanalyzer after 12 hour fasting. GFR was calculated based on serum creatinine. Mean (±SD) of serum creatinine, cholesterol, triglyceride, HDL and LDL levels and GFR were 1.04±0.16, 201±41, 153±125, 43±10, 128±37 mg/dl and 99±19 ml/min respectively. Subjects with saturated fatty acids intake of more than 10 percent of calorie had higher levels of serum creatinine versus the others (P<0.01, 10.8±0.009 vs 1.03±0.2). People with oleic and linoleic acid intake of more than 10% and 1% of calorie ,respectively, had higher levels of serum creatinine and GFR (p<0.01). There were significant correlations between serum cholesterol, LDL, HDL, and triglyceride with serum creatinine (p<0.05, p<0.05, p<0.001, p<0.001 respectively). According to observed correlations the control of intake of desirable amount of fatty acids and serum lipid and lipoproteins level is necessary for renal health.