Journal of Kerman University of Medical Sciences

Determination of electrophoretype of rotaviruses causative agents of diarrhea in children under two years oid referred to Tehran and Zahedan hospitals

Document Type : Original Article

Authors

M Saeb 1
A.V Moradi 2
T Mokhtari azad 1
J Sajadian fard

1 Associate professor

2 Instrutor

Abstract
rotavirus infections are one of the etiological agents of infantile and children,s gastroenteritis.in this study 450 fecal samples from children under 2 years old from cities of Tehran nad Zahedan were collected.of these specimens.73 samples which were cosidered as moderately positive(++) by ELISA were selected.SDS-PAGE analysis showed fifty six different rotavirus electrophoretypes from which eight and forth eight patterns belonged to  Zahedan and Tehran samples respectively.basically,from eight patterns of Zahedan samples,five(71.5%) were related to L and two (28.5%) to S rotavirus group A which includes seven different electrophoretypes.from forty eight patterns of Tehran samples,thirth six belonged to L series with twenty six defferent electrophoretypes (76.5) and twelve to S types with eight different electrophoretypes (23.5%).in addition, a mixed S and L electrophoretype and two abnormal genom of rotavirus with the pattern of 2&3&3&4 were identified.different electrophoretype of rotaviruses in Tehran and Zahedan showed a high diversity in comparison with electrophoretic patterns from other countries.
 

Keywords

roravirus
infantile diarrhea
electrophoretype
viral diseases
Journal of Kerman University of Medical Sciences
Volume 4, Issue 3
1997
Pages 101-106

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