Larvicidial Effects of essential oil and methanol extract of Achilleawilhelmsii C. Koch (Asteraceae) against Anopheles stephensi Liston (Diptera: Culicidae), a malaria vector

Soleimani-Ahmadi, Moussa; Gorouhi, Mohammad Amin; Mohammadi Azani, Sadegh; SalimAbadi, Yaser; Paksa, Azim; Rashid, Golsoom; Sanei-Dehkordi, Alireza

Larvicidial Effects of essential oil and methanol extract of Achilleawilhelmsii C. Koch (Asteraceae) against Anopheles stephensi Liston (Diptera: Culicidae), a malaria vector

Document Type : Original Article

Authors

Moussa Soleimani-Ahmadi ¹

Mohammad Amin Gorouhi ²

Sadegh Mohammadi Azani ³

Yaser SalimAbadi ⁴

Azim Paksa ⁵

Golsoom Rashid ⁶

Alireza Sanei-Dehkordi ⁷

¹ Associate Professor, Department of Medical Entomology and Vector Control, Faculty of Health &Infectious and Tropical Diseases Center,Hormozgan University of Medical Sciences, Bandar Abbas, Iran

² Assistant Professor, Department of Medical Entomology and Vector Control, School of Health, Kerman University of Medical Sciences, Kerman, Iran

³ Research Assistant, Faculty of Health, Semnan University of Medical Sciences, Semnan, Iran

⁴ Assistant Professor, Department of Health Services and Health Promotion, School of Health& Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

⁵ Postgraduate student, Department of Medical Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

⁶ Postgraduate student, Department of Medical Entomology and Vector Control, Faculty of Health, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

⁷ AssistantProfessor, Department of Medical Entomology and Vector Control, Faculty of Health& Infectious and Tropical Diseases Research Center,Hormozgan University of Medical Sciences, Bandar Abbas, Iran

Abstract

Background: Mosquitoes are responsible for the transmission of many pathogens and parasites and consequently serious diseases in humans. Currently, application of plant derivatives has been suggested as an alternative bio-control technique for these medically important vectors.
Methods: In this study the essential oil and methanol extract of Achilleawilhelmsii were tested against late-3^rd or young-4^th instar larvae stages of mosquito vector,Anopheles stephensi, under laboratory condition. The larval mortality was calculated after 24 h of the exposure period. Data were subjected to Probit analysis in order to estimate the lethal concentration for 50% and 90% of mortality values.
Results: Results showed that the essential oil induced 100% larval mortality of An. stephensilarvae after 24 h with a dosage of 160 ppm, However, a dosage of 320 ppm of methanol extract was required to reach 100% larval mortality.Theessential oil methanol extract exerted significant larvicidal activity with LC₅₀ values of 39.04 and 115.73 ppm, respectively.
Conclusion: Our finding suggests that A. wilhelmsii oil is a potential source and has valuable larvicidal compounds for mosquito larval control.

Keywords

Achilleawilhelmsii

Anophelesstephensi

Larvicidal effect

Essential oil

Methanol extract

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