Journal of Kerman University of Medical Sciences

The Relationship between Hepatitis C Genotypes and Viral Load: a study on hepatitis C patients in Kerman, Iran using Real-time PCR

Document Type : Original Article

Authors

Farideh Doostan 1
Marziye Jahanshahi Jahanshahi 2
Farida Hassanzadeh 3
Mohammadreza Bazrafshani 4

1 Assistant Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences & Department of Nutrition, Kerman University of Medical Sciences, Kerman, Iran

2 MSc. Student, Department of Microbiology, Islamic Azad University, Kerman, Iran

3 BSc. in Molecular and Cellular Biology-Genetic, Dr. Bazrafshani Medical Genetic Lab, Kerman, Iran

4 Assistant Professor, Department of Medical Genetics, Kerman University of Medical Sciences, Kerman, Iran

Abstract
Abstract

Background: Distribution of hepatitis C virus genotypes varies geographically and can be associated with clinical aspects. The virus has six major genotypes and eleven sub-genotypes. According to the recent studies, in hepatitis C patients, treatment duration and ribavirin dosage should be determined based on the type of virus genotype. Because of contradictory results reported in different countries and races, the present study aimed to determine the genotype of hepatitis C and its relationship with viral load in HCV patients in Kerman, Iran.
Methods: The study included 106 patients with hepatitis C referred to Dr. Bazrafshani Medical Genetic Lab. in Kerman. Patients’ blood plasma was collected and used for virus genotyping and viral load determination by Real-Time PCR technique.
Results: From 106 studied patients, 82 ones were male (77.4%) and 24 ones were female (22.6%). Most frequent genotypes were respectively genotype 3 (55.7%) and genotype 1(44.3%). Viral load in genotype1 was greater than that in genotype 3 (P=0.011).
Conclusions: In this study, the predominant genotype was genotype 3. Therefore, treatment strategies should go towards type 3 virus. On the other hand, copy number of genotype 1virus was higher than that of type 3 that should be considered very important in determining the treatment duration in HCV patients.
 

Keywords

Hepatitis C Virus
Genotype
Viral load
Real-Time PCR
  1. Vezali E, Aghemo A, Colombo M. A review of the treatment of chronic hepatitis C virus infection in cirrhosis. Clin Ther 2010; 32(13): 2117-38.
  2. Irshad M, Ansari MA, Singh A, Nag P, Raghvendra L, Singh S, Badhal SS. Hcv-genotypes: A review on their origin, global status, assay system, pathogenecity and response to treatment. Hepato Gastroenterology 2010; 57(104):1529-38.
  3. Keyvani H. Virology of Hepatitis C. Hepatitis B and C Seminar Oct 30 2008. Naft Hospital, Tehran, Iran.
  4. Ali Asadi Hashtroudi. Iranian Medical information database.
  5. Zali MR, Mayumi M, Raoufi M, Nowroozi A. Hepatitis C virus genotypes in the Islamic Republic of Iran: a preliminary study. East Mediterr Health 2000; 6(2-3):372-7.
  6. Samimi-Rad K, Nategh R, Malekzadeh R, Norder H, Magnius L. Molecular epidemiology of hepatitis Cvirus in Iran as reflected by phylogenetic analysis of the NS5B region. J Med Virol2004; 74(2):246-52.
  7. Harris KA, Gilham C, Mortimer PP, Teo CG. The most prevalent hepatitis C virus genotypes in England and Wales are 3a and 1a. J Med Virol 1999; 58(2):127-31.
  8. Ohno T, Mizokami M, Saleh MG, et al. Usefulness and limitation of phylogenetic analysis for hepatitis C virus core region: application to isolates from Egyptian and Yemeni patients. Arch Virol 1996; 141(6):1101-13.
  9. Alavian S, Sali S. A review of the Hepatitis C and how to deal with it. Journal of Medical Council of Islamic Republic of Iran 2000; 18(2): 49-65.
  10. Khaliq S, Jahan S, Pervaiz A. Sequence variability of HCV core region: important predictors of HCV induced pattogenesis and viral psduction. Infect genet Evol 2011; 11(3): 543-56.
  11. Von Wagner M, Huber M, Berg T, et al. Peginterferon-alpha-2a (40KD) and ribavirin for 16 or 24 weeks in patients with genotype 2 or 3 chronic hepatitis C. Gastroenterology 2005;129(2):522-7.
  12. Rodriguez JC, Garcia J, Moya I, Ayelo A, Vazquez N, Sillero C, Royo G. Genetic variability of hepatitis C virus in the health area of Elche (Spain). Correlation between core antigen and viral load. GastroenterolHepatol2003; 26(7): 407-10.
  13. Garcia F, Roldan C, Hernandez-Quero J, Bernal MC, Martinez MA, Lopez MA, Piedrola G, Maroto MC. Relationship between viral genotype and viral load in patients with chronic hepatitis C. Eur J ClinMicrobiol Infect Dis 1996; 15(11):884-7.
  14. Berg T, Hopf U, Stark K, Baumgarten R, Lobeck H, Schreier E. Distribution of hepatitis C virus genotypes in German patients with chronic hepatitis C: correlation with clinical and virological parameters. J Hepatol 1997; 26(3):484-91.
  15. Booth JC, O’grady J, Neuberger J. Clinical guidelines on the management of hepatitis C. Gut, 2001; 49 Suppl 1, I1-21.

16-       Molaabedin M, Pedarzadeh M. Study of Various HCV Genotypes in Patients Managing by Referral Clinic in Yazd Province. Journal of Medical Sciences Shahid Sadoughi yazd. 2012; 19(6): 784-790.

17-       Sarvghad M, Bejadi A, Farrokhneia M, Javanian M. Study of hepatitis c virus genotypes in patients with chronic hepatitis in Mashhad]. Med J Mashad Univ Med Sci 2006; 49(93): 309–14.

18-       Manns MP, Wedemeyer H, Cornberg M. Treating viral hepatitis C: efficacy, side effects, and complications. Gut 2006; 55(9): 1350-9.

19-       Watanabe K, Yoshioka K, Yano M, Ishigami M, Ukai K, Ito H, Miyata F, Mizutani T, Goto H. Mutations in the nonstructural region 5B of hepatitis C virus genotype 1b: their relation to viral load, response to interferon, and the nonstructural region 5A. J Med Virol 2005; 75(4): 504-12.

20-       Chakravarti A, Dogra G, Verma V, Srivastava AP. Distribution pattern of HCV genotypes & its association with viral load. Indian J Med Res 2011; 133: 326-31.

21-       Rodriguez JC, Garcia J, Moya I, Ayelo A, Vazquez N, Sillero C, Royo G. Genetic variability of hepatitis C virus in the health area of Elche (Spain), Correlation between core antigen and viral load. GastroenterolHepatol2003; 26(7): 407-10.

22-       Singh P, BhatiaV, Pandey M, Shashank M, Tidke P, Jha N, Dutt S. HCV Genotypes Distribution Pattern & ITS Association with Viralloadinindia. International J ournal of Recent Scien Researchs .2013; 4(11): 1682-84.

23-       Hadinedoushan H, Salmanroghani H, Amirbaigy MK, Akhondi-Meybodi M. Hepatitis C Virus Genotypes and Association with Viral Load in Yazd, Central Province of Iran. Hepat Mon 2014; 14(3): e11705.

24-      Alizadeh A, Keyvani H, Ranjbar M. Prevalence of hepatitis C virus genotypes in patients with chronic hepatitis C virus in Tehran. Journal of Infectious Diseases and Tropical Medicine. 2008; 13 (40):15-19.

Journal of Kerman University of Medical Sciences
Volume 24, Issue 2
2017
Pages 159-165

Article View 7,697
PDF Download 15,864

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