Journal of Kerman University of Medical Sciences

Neuroprotective effects of voluntary wheel running and eriobotrya japonica flower extract on Parkinsonian rats

Document Type : Original Article

Authors

Hossein Shirvani 1
Hossein TaheriChadorneshin 2
Hadi Sarir 3
Jalil Aslani 4

1 Assistant Professor, Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Assistant Professor, Department of Sport Sciences, University of Bojnord, Bojnord, Iran

3 Associate Professor, Department of Animal Science, University of Birjand, Birjand, Iran

4 Instructor, Exercise Physiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Abstract
Backgrounds: The loss of dopamine-secreting cells and the decrease in the status of antioxidant is associated with incidents of Parkinson's disease. The purpose of the present study was to determine the protective effect of a 12- weeks voluntary wheel running (VWR) along with the injection of eriobotrya japonica flower extract (EJFE, 200 mg/kg body weight, 3 days a week) on cerebral dopamine neurotrophic factor (CDNF), superoxide dismutase (SOD), and malondialdehyde (MDA) in the cerebral cortex of a rat model of Parkinson’s disease (PD).
Method: To do so, the rats were trained for 12 weeks with and without EJFE prior to the induction of Parkinson. In order to obtain the Parkinsonian model, 6-hydroxydopamine (6-OHDA) (5 µL) was injected intracerebrally. Data were statistically analyzed by one-way analysis of variance followed by LSD post-hoc test (P < 0.05).
Results: 6-OHDA injection significantly decreased the CDNF contents, and SOD activity while it increased MDA levels in cerebral cortex of the Parkinsonian control group. The pre-training of PD rats with and without EJFE increased the CDNF content and SOD activity and also decreased MDA levels.
Conclusion: Preconditioning by VWR and EJFE may be effective in reducing the consequences of toxins resulted in Parkinson's disease.
 

Keywords

Parkinson's disease
Voluntary wheel running
Eriobotrya japonica flower extract
Cerebral dopamine neurotrophic factor
Superoxide dismutase
Malondialdehyde
  1. Klamroth S, Steib S, Devan S, Pfeifer K. Effects of Exercise Therapy on Postural Instability in Parkinson Disease: A Meta-analysis. Journal of Neurologic Physical Therapy 2016; 40(1): 3-14.
  2. Lindholm P, Voutilainen MH, Laurén J, Peränen J, Leppänen V-M, Andressoo J-O, et al. Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo. Nature 2007; 448(7149): 73-7.
  3. Nadella R, Voutilainen MH, Saarma M, Gonzalez-Barrios JA, Leon-Chavez BA, Jiménez JMD, et al. Transient transfection of human CDNF gene reduces the 6-hydroxydopamine-induced neuroinflammation in the rat substantia nigra. Journal of Neuroinflammation 2014; 11(1): 209.
  4. Huang Y, Li J, Cao Q, Yu S-C, Lv X-W, Jin Y, et al. Anti-oxidative effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf in chronic bronchitis rats. Life sciences 2006; 78(23): 2749-57.
  5. Tieu K, Ischiropoulos H, Przedborski S. Nitric oxide and reactive oxygen species in Parkinson's disease. IUBMB life 2003; 55(6): 329-35.
  6. Dávalos A, Bartolomé B, Gómez-Cordovés C. Antioxidant properties of commercial grape juices and vinegars. Food chemistry 2005; 93(2): 325-30.
  7. Mabandla MV, Kellaway LA, Daniels WM, Russell VA. Effect of exercise on dopamine neuron survival in prenatally stressed rats. Metabolic Brain Disease 2009; 24(4): 525-39.
  8. Yoon M-C, Shin M-S, Kim T-S, Kim B-K, Ko I-G, Sung Y-H, et al. Treadmill exercise suppresses nigrostriatal dopaminergic neuronal loss in 6-hydroxydopamine-induced Parkinson's rats. Neuroscience Letters 2007; 423(1): 12-7.
  9. Grillo CA, Piroli GG, Rosell DR, Hoskin EK, Mcewen BS, Reagan LP. Region specific increases in oxidative stress and superoxide dismutase in the hippocampus of diabetic rats subjected to stress. Neuroscience 2003; 121(1): 133-40.
  10. Esmaeili AH, Khavari-Nejad RA, Moghaddam AH, Chaichi MJ, Ebrahimzadeh MA. Effects of Eriobotrya japonica (Lindl.) flower extracts on mercuric chloride-induced hepatotoxicity in rats. Chinese Science Bulletin 2012; 57(30): 3891-7.
  11. Hamada A, Yoshioka S, Takuma D, Yokota J, Cui T, Kusunose M, et al. The effect of Eriobotrya japonica seed extract on oxidative stress in adriamycin-induced nephropathy in rats. Biol Pharm Bull 2004; 27(12): 1961-4.
  12. Poulton NP, Muir GD. Treadmill training ameliorates dopamine loss but not behavioral deficits in hemi-parkinsonian rats. ExpNeurol 2005; 193(1): 181-97.
  13. Wu S-Y, Wang T-F, Yu L, Jen CJ, Chuang J-I, Wu F-S, et al. Running exercise protects the substantia nigra dopaminergic neurons against inflammation-induced degeneration via the activation of BDNF signaling pathway. Brain Behav Immun 2011; 25(1): 135-46.
  14. Lau YS, Patki G, Das‐Panja K, Le WD, Ahmad SO. Neuroprotective effects and mechanisms of exercise in a chronic mouse model of Parkinson’s disease with moderate neurodegeneration. European Journal of Neuroscience 2011; 33(7): 1264-74.
  15. Nishioka Y, Yoshioka S, Kusunose M, Cui T, Hamada A, Ono M, et al. Effects of extract derived from Eriobotrya Japonica on liver function improvement in rats. Biological and Pharmaceutical Bulletin. 2002; 25(8): 1053-7.
  16. Budantsev Alu, Kisliuk OS, Shul'govskiĭ VV, Rykunov DS, Iarkov AV. The brain in stereotaxic coordinates (a textbook for colleges). Zh Vyssh Nerv Deiat IM I P Pavlova 1993; 43(5): 1045-51.
  17. Sharma R, McMillan CR, Niles LP. Neural stem cell transplantation and melatonin treatment in a 6‐hydroxydopamine model of Parkinson’s disease. Journal of Pineal Research 2007; 43(3): 245-54.
  18. Nezhadi A, Sheibani V, Esmaeilpour K, Shabani M, Esmaeili-Mahani S. Neurosteroid allopregnanolone attenuates cognitive dysfunctions in 6-OHDA-induced rat model of Parkinson's disease. Behav Brain Res 2016; 305: 258-64.
  19. Sachs C, Jonsson G. Mechanisms of action of 6-hydroxydopamine. Biochemical pharmacology1975; 24(1): 1-8.
  20. Van Kampen J, Robertson H, Hagg T, Drobitch R. Neuroprotective actions of the ginseng extract G115 in two rodent models of Parkinson's disease. Exp Neurol 2003; 184(1): 521-9.
  21. Kono Y. Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Archives of Biochemistry and Biophysics 1978; 186(1): 189-95.
  22. Draper HH, Hadley M. [43] Malondialdehyde determination as index of lipid Peroxidation. Methods in enzymology 1990; 186: 421-31.
  23. Radak Z, Toldy A, Szabo Z, Siamilis S, Nyakas C, Silye G, et al. The effects of training and detraining on memory, neurotrophins and oxidative stress markers in rat brain. Neurochem Int 2006; 49(4): 387-92.
  24. TaheriChadorneshin H, Cheragh-Birjandi S, Ramezani S, Abtahi-Eivary SH. Comparing sprint and endurance training on anxiety, depression and its relation with brain-derived neurotrophic factor in rats. Behav Brain Res 2017; 329: 1-5.
  25. Lohoff FW, Bloch PJ, Ferraro TN, Berrettini WH, Pettinati HM, Dackis CA, et al. Association analysis between polymorphisms in the conserved dopamine neurotrophic factor (CDNF) gene and cocaine dependence. Neuroscience Letters 2009; 453(3): 199-203.
  26. Choi JM, Hong JH, Chae MJ, Hung NP, Kang HS, Ma HI, et al. Analysis of mutations and the association between polymorphisms in the cerebral dopamine neurotrophic factor (CDNF) gene and Parkinson disease. Neuroscience Letters 2011; 493(3): 97-101.
  27. Yokota J, Takuma D, Hamada A, Onogawa M, Yoshioka S, Kusunose M, et al. Scavenging of reactive oxygen species by Eriobotrya japonica seed extract. Biological and Pharmaceutical Bulletin 2006; 29(3): 467-71.
  28. Ding C-K, Chachin K, Ueda Y, Imahori Y, Wang CY. Metabolism of phenolic compounds during loquat fruit development. J Agric Food Chem 2001; 49(6): 2883-8.
Journal of Kerman University of Medical Sciences
Volume 24, Issue 4
2017
Pages 289-297

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