Journal of Kerman University of Medical Sciences

Comparative Evaluation of Immunohistochemical Expression of Endothelin A Receptor between Oral Squamous Cell Carcinoma and Normal Oral Mucosa

Document Type : Short Communication

Authors

Elahe Mehranfar 1
Forough Foroughi 2
Hemmat Gholinia 3
hamid abbaszadeh 4

1 Student Research Committee, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran

2 Assistant Professor, Department of Pathology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Health Research Institute, Babol University of Medical Sciences, Babol, Iran

4 Assistant Professor, Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

Abstract
Background: Recent research has provided evidences indicating the importance of endothelin axis in carcinogenesis. According to our knowledge, there are little information about endothelin A receptor (ETA) expression in oral squamous cell carcinoma (OSCC). So, the aim of the present study was to evaluate the immunohistochemical expression of ETA in OSCC and normal oral mucosa (NOM).
Methods:In this cross-sectional study, studied group composed of paraffin-embedded tissue blocks of 21 OSCCs and 20 NOMs. Four micron sections were prepared from tissue blocks and stained with ETA antibody using immunohistochemistry (IHC). Percentage of stained cells and staining intensity were compared between OSCC and NOM groups and also between different grades of OSCC using Mann-Whitney, Chi-Square and Kruskal-Wallis statistical tests.
Results:In OSCC group, all cases showed positive staining  for ETA while in NOM group, 17 cases showed no staining. Comparison of the percentage of stained cells and staining intensity for ETArevealed a significant difference between OSCC and NOM groups (p <0.001). There was also a significant difference between different grades of OSCC with respect to the percentage of stained cells (P=0.01) so that with increase in grade, ETA expression was also increased. 
Conclusion:The results of this study support the role of ETA receptor in carcinogenesis process and progression of OSCC.

Keywords

oral squamous cell carcinoma
Normal mucosa
Endothelin A receptor
  1. Hoffmann RR, Yurgel LS, Campos MM. Endothelins and their receptors as biological markers for oral cancer. Oral Oncol 2010; 46(9):644-7.
  2. Alaizari NA, Abdelbary SN, Amin NR. Immunohistochemical expression of endothelin protein in oral squamous cell carcinoma. Indian J Pathol Microbiol 2013; 56(2):151-4.
  3. Ishimoto S, Wada K, Tanaka N, Yamanishi T, Ishihama K, Aikawa T, et al. Role of endothelin receptor signalling in squamous cell carcinoma. Int J Oncol 2012; 40(4):1011-9.
  4. Bagnato A, Natali PG. Endothelin receptors as novel targets in tumor therapy. J Transl Med 2004; 2(1):16.
  5. Hoffmann RR, Yurgel LS, Campos MM. Evaluation of salivary endothelin-1 levels in oral squamous cell carcinoma and oral leukoplakia. Regul Pept 2011; 166(1-3):55-8.
  6. Neville BW, Daam DD, Allen CM, Bouqout JE. Oral and Maxillofacial Pathology. 4th ed. Missouri: Saunders; 2016.
  7. Wulfing P, Tio J, Kersting C, Sonntag B, Buerger H, Wulfing C, et al. Expression of endothelin-A-receptor predicts unfavourable response to neoadjuvant chemotherapy in locally advanced breast cancer. Br J Cancer 2004; 91(3):434-40.
  8. Salem SA, Gamal Aly D, Salah Youssef N, Moneim El-Shaer MA. Immunohistochemical assessment of endothelin-1 axis in psoriasis, basal cell carcinoma and squamous cell carcinoma. G Ital Dermatol Venereol 2015; 150(3):283-91.
  9. Ishibashi Y, Hanyu N, Nakada K, Suzuki Y, Yamamoto T, Takahashi T, et al. Endothelin protein expression as a significant prognostic factor in oesophageal squamous cell carcinoma. Eur J Cancer 2003; 39(10):1409-15.
  10. Awano S, Dawson LA, Hunter AR, Turner AJ, Usmani BA. Endothelin system in oral squamous carcinoma cells: specific siRNA targeting of ECE-1 blocks cell proliferation. Int J Cancer 2006; 118(7):1645-52.
  11. Pickering V, Jordan RC, Schmidt BL. Elevated salivary endothelin levels in oral cancer patients--a pilot study. Oral Oncol 2007; 43(1):37-41.
  12. Hinsley EE, Hunt S, Hunter KD, Whawell SA, Lambert DW. Endothelin-1 stimulates motility of head and neck squamous carcinoma cells by promoting stromal-epithelial interactions. Int J Cancer 2012; 130(1):40-7.
  13. Cong N, Li Z, Shao W, Li J, Yu S. Activation of ETA receptor by endothelin-1 induces hepatocellular carcinoma cell migration and invasion via ERK1/2 and AKT signaling pathways. J Membr Biol 2016; 249(1-2):119-28.
Journal of Kerman University of Medical Sciences
Volume 25, Issue 1
January and February 2018
Pages 84-92

Home

Editorial Board

Guide for Authors

Submit Manuscript

Guide for Reviewers

Contact Us