SNHG6 203 and SNHG6 201 Transcripts Can be Used as Contributory Factors for a Well-Timed Prognosis and Diagnosis of Colorectal Cancer

Document Type : Original Article


Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran


Background:Long non-coding RNAs, as a big part of non-coding RNAs, are considered functionally more than past. These transcripts could be involved in carcinogenesis. SNHG6, as a long non-coding RNA, has been reported to be expressed more in colorectal cancer tissues than non-cancerous ones.  Colorectal cancer as a malignancy needs fast prognostic and diagnostic methods for well-timed treatment. SNHG6 RNA and its relative variants can be considered as biomarkers for a well-timed treatment of colorectal cancer.
Methods:RNA extraction from 32 colorectal cancer tissues and their relative non-cancerous tissues were carried out and cDNA of the mentioned RNAs was synthesized and RT-qPCR was performed. Relative expression of SNHG6 201 and 203 were studied in colorectal cancer samples with different clinicopathological characteristics.
Results:The expression patterns of SNHG6 201 and 203 variants were different. SNHG6 203 was expressed significantly higher in colorectal tumor tissues than non-cancerous ones. In spite of SNHG6 203, SNHG6 201 was expressed significantly in colorectal non-cancerous tissues more than tumor ones. Additionally, expression of these variants in different colorectal cancer cell lines was different.
Conclusion:It seems that SNHG6 203 transcript might be considered as a prognostic and diagnostic biomarker in colorectal cancer case studies and treatments. Also, SNHG6 201 can distinguish precisely the tumor and non-tumor tissues in colorectal cancer.


  1. Kitagawa M, Kitagawa K, Kotake Y, Niida H, Ohhata T. Cell cycle regulation by long non-coding RNAs. Cell Mol Life Sci 2013; 70(24):4785-94.
  2. Rossi MN, Antonangeli F. LncRNAs: new players in apoptosis control. Int J Cell Biol 2014; 2014:473857.
  3. Degirmenci U, Lei S. Role of lncRNAs in cellular aging. Front Endocrinol (Lausanne) 2016; 7:151.
  4. Zhao W, Fu H, Zhang S, Sun S, Liu Y. LncRNA SNHG16 drives proliferation, migration, and invasion of hemangioma endothelial cell through modulation of miR-520d-3p/STAT3 axis. Cancer Med 2018; 7(7):3311-20.
  5. Huang Z, Ye B, Wang Z, Han J, Lin L, Shan P, et al. Inhibition of LncRNA-HRIM increases cell viability by regulating autophagy levels during hypoxia/reoxygenation in myocytes. Cell Physiol Biochem 2018; 46(4):1341-51.
  6. Dhamija S, Diederichs S. From junk to master regulators of invasion: lncRNA functions in migration, EMT and metastasis. Int J Cancer 2016; 139(2):269-80.
  7. Yu X, Li Z. Long non-coding RNA HOTAIR: a novel oncogene (Review). Mol Med Rep 2015; 12(4):5611-18.
  8. Hokii Y, Sasano Y, Sato M, Sakamoto H, Sakata K, Shingai R, et al. A small nucleolar RNA functions in rRNA processing in Caenorhabditis elegans. Nucleic Acids Res 2010; 38(17):5909-18.
  9. Kiss T. Small nucleolar RNA‐guided post‐transcriptional modification of cellular RNAs. EMBO J 2001; 20(14):3617-22.
  10. Gerbi SA, Borovjagin AV. Pre-ribosomal RNA Processing in Multicellular Organisms. Texas: Landes Bioscience; 2000-2013. p.170-98.
  11. Sloan KE, Warda AS, Sharma S, Entian KD, Lafontaine DL Bohnsack MT. Tuning the ribosome: the influence of rRNA modification on eukaryotic ribosome biogenesis and function. RNA Biol 2017; 14(9):1138-52.
  12. Gerbi SA, Borovjagin AV, Ezrokhi M, Lange TS. Ribosome biogenesis: role of small nucleolar RNA in maturation of eukaryotic rRNA. Cold Spring Harb Symp Quant Biol 2001; 66:575-90.
  13. Nazar R. Ribosomal RNA processing and ribosome biogenesis in eukaryotes. IUBMB life 2004; 56(8):457-65.
  14. Penzo M, Montanaro L. Turning uridines around: role of rRNA pseudouridylation in ribosome biogenesis and ribosomal function. Biomolecules 2018; 8(2):E38.
  15. Donati G, Montanaro L, Derenzini M. Ribosome Biogenesis and control of cell proliferation: p53 is not alone. Cancer Res 2012; 72(7):1602-7.
  16. Pelletier J, Thomas G, Volarevic S. Ribosome biogenesis in cancer: new players and therapeutic avenues. Nat Rev Cancer 2017; 18(1):51-63.
  17. Bastide A, David A. The ribosome, (slow) beating heart of cancer (stem) cell. Oncogenesis 2018; 7(4):34.
  18. Chang L, Yuan Y, Li C, Guo T, Qi H, Xiao Y, et al. Upregulation of SNHG6 regulates ZEB1 expression by competitively binding miR-101-3p and interacting with UPF1 in hepatocellular carcinoma. Cancer Lett 2016; 383(2):183-94.
  19. Yang T, Zhou H, Liu P, Yan L, Yao W, Chen K, et al. lncRNA PVT1 and its splicing variant function as competing endogenous RNA to regulate clear cell renal cell carcinoma progression. Oncotarget 2017; 8(49):85353-67.
  20. Birzele F, Csaba G, Zimmer R. Alternative splicing and protein structure evolution. Nucleic Acids Res 2008; 36(2):550-8.
  21. Black DL. Protein diversity from alternative splicing: a challenge for bioinformatics and post-genome biology. Cell 2000; 103(3):367-70.
  22. Shahryari A, Rafiee MR, Fouani Y, Oliae NA, Samaei NM, Shafiee M, et al. Two novel splice variants of SOX2OT, SOX2OT‐S1, and SOX2OT‐S2 are coupregulated with SOX2 and OCT4 in esophageal squamous cell carcinoma. Stem cells 2014; 32(1):126-34.
  23. Cao C, Zhang T, Zhang D, Xie L, Zou X, Lei L, et al. The long non-coding RNA, SNHG6-003, functions as a competing endogenous RNA to promote the progression of hepatocellular carcinoma. Oncogene 2017; 36(8):1112-22.
  24. Yan K, Tian J, Shi W, Xia H, Zhu Y. LncRNA SNHG6 is associated with poor prognosis of gastric cancer and promotes cell proliferation and EMT through epigenetically silencing p27 and sponging miR-101-3p. Cell Physiol Biochem 2017; 42(3):999-1012.
  25. Meng Q, Yang BY, Liu B, Yang J-X, Sun Y. Long non-coding RNA SNHG6 promotes glioma tumorigenesis by sponging miR-101-3p. Int J Biol Markers 2018; 33(2):148-55.