Journal of Kerman University of Medical Sciences

Reduction of Saliva and Serum 25-Hydroxycholecalciferol in Multiple Sclerosis

Document Type : Original Article

Authors

Mohammad-Reza Mirzaii-Dizgah 1
Mohammad-Hosein Mirzaii-Dizgah 2
Iraj Mirzaii-Dizgah 3

1 Student Research Committee, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

2 Student Research Committee, School of Dentistry, Aja University of Medical Sciences; Tehran, Iran

3 Professor Department of Physiology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran

https://doi.org/10.22062/jkmu.2020.90613
Abstract
Methods:The prevalence of multiple sclerosis (MS) is high in patients suffering from 25-hydroxyvitamin D3 [25(OH)D] deficiency. The aim of this study was to investigate the possible association of saliva and serum 25(OH)D concentrations with MS in women.
Methods: Serum and saliva 25(OH)D levels of 30 MS women and 30 matched healthy controls in this case-control study were measured by ELISA. Data were analyzed by unpaired two-tailed student’s t-test, Pearson correlation test and Receiver operating characteristic (ROC).
Results: The mean levels of 25(OH)D in serum and in both stimulated and unstimulated saliva were significantly lower in patients with MS. Serum levels of 25(OH) significantly correlated with stimulated (r = 0.575; P=0.003) and unstimulated saliva (r = 0.548; P=0.004). The mean (±SD) EDSS was 3.6±1.9 in the MS group. EDSS was not significantly correlated with 25(OH)D in serum or in stimulated and unstimulated saliva. The cut-off points of 25(OH)D in serum and saliva were 18 ng/ml and 65 pg/ml, respectively.
Conclusions:25(OH)D level in saliva like in serum was low in MS women. Serum 25(OH)D levels correlated positively with saliva 25(OH)D in women suffering from MS.

Keywords

Multiple sclerosis 25
Hydroxycholecalciferol Saliva
  1. Harirchian MH, Fatehi F, Sarraf P, Honarvar NM, Bitarafan S. Worldwide prevalence of familial multiple sclerosis: a systematic review and meta-analysis. Mult Scler Relat Disord 2018; 20:43-47.
  2. Eskandarieh S, Molazadeh N, Moghadasi AN, Azimi AR, Sahraian MA. The prevalence, incidence and familial recurrence of multiple sclerosis in Tehran, Iran. Mult Scler Relat Disord 2018; 25:143.
  3. Bettencourt A, Boleixa D, Reguengo H, Samões R, Santos E, Oliveira JC, et al. Serum 25-hydroxyvitamin D levels in multiple sclerosis patients from the north of Portugal. J Steroid Biochem Mol Biol 2018; 180:137-141.
  4. Smolders J, Damoiseaux J. Vitamin D as a T-cell modulator in multiple sclerosis. Vitam Horm 2011; 86:401-28.
  5. Szymczak I, Pawliczak R. The active metabolite of vitamin D3 as a potential immunomodulator. Scand J Immunol 2016; 83(2):83-91.
  6. Ashtari F, Toghianifar N, Zarkesh-Esfahani SH, Mansourian M. Short-term effect of high-dose vitamin D on the level of interleukin 10 in patients with multiple sclerosis: a randomized, double-blind, placebo-controlled clinical trial. Neuroimmunomodulation 2015; 22(6):400-4.
  7. D'Ambrosio D, Cippitelli M, Cocciolo MG, Mazzeo D, Di Lucia P, Lang R, et al. Inhibition of IL-12 production by 1,25-dihydroxyvitamin D3. Involvement of NF-kappaB downregulation in transcriptional repression of the p40 gene. J Clin Invest 1998; 101(1):252-62.
  8. da Costa DS, Hygino J, Ferreira TB, Kasahara TM, Barros PO, Monteiro C, et al. Vitamin D modulates different IL-17-secreting T cell subsets in multiple sclerosis patients. J Neuroimmunol 2016; 299:8-18.
  9. Haas J, Schwarz A, Korporal-Kuhnke M, Faller S, Jarius S, Wildemann B. Hypovitaminosis D upscales B-cell immunoreactivity in multiple sclerosis. J Neuroimmunol 2016; 294:18-26.
  10. Zhang Y, Liu G, Han X, Dong H, Geng J. The association of serum 25-hydroxyvitamin D levels with multiple sclerosis severity and progression in a case-control study from China. J Neuroimmunol. 2016; 297:127-31.
  11. Karampoor S, Zahednasab H, Ramagopalan S, Mehrpour M, Safarnejad Tameshkel F, Keyvani H. 25-hydroxyvitamin D levels are associated with multiple sclerosis in Iran: a cross-sectional study. J Neuroimmunol 2016; 290:47-8.
  12. Duan S, Lv Z, Fan X, Wang L, Han F, Wang H, et al. Vitamin D status and the risk of multiple sclerosis: a systematic review and meta-analysis. Neurosci Lett 2014; 570:108-13.
  13. Munger KL, Levin LI, Hollis BW, Howard NS, Ascherio A. Serum 25-hydroxyvitamin D levels and risk of multiple sclerosis. JAMA 2006; 296(23):2832-8.
  14. Polman CH, Reingold SC, Banwell B, Clanet M, Cohen JA, Filippi M, et al. Diagnostic criteria for multiple sclerosis: 2010 revisions to the McDonald criteria. Ann Neurol 2011; 69(2):292-302.
  15. Manconi B, Liori B, Cabras T, Vincenzoni F, Iavarone F, Lorefice L, et al. Top-down proteomic profiling of human saliva in multiple sclerosis patients. J Proteomics 2018; 187:212-22.
  16. Pietz K, Haas J, Wurster U. Protein composition, IgG, and IgA analysis in the saliva of patients with multiple sclerosis. Ann N Y Acad Sci 1993; 694:305-7.
  17. Agha-Hosseini F, Mirzaii-Dizgah I, Mirjalili N. Relationship of unstimulated saliva cortisol level with severity of oral dryness feeling in menopausal women. Aust Dent J 2011; 56(2):171-4.
  18. Kurtzke JF. Rating neurologic impairment in multiple sclerosis: an expanded disability status scale (EDSS). Neurology 1983; 33(11):1444-52.
  19. Soilu-Hänninen M, Laaksonen M, Laitinen I, Erälinna JP, Lilius EM, Mononen I. A longitudinal study of serum 25-hydroxyvitamin D and intact parathyroid hormone levels indicate the importance of vitamin D and calcium homeostasis regulation in multiple sclerosis. J Neurol Neurosurg Psychiatry 2008; 79(2):152-7.
  20. Soilu-Hänninen M, Airas L, Mononen I, Heikkilä A, Viljanen M, Hänninen A. 25-Hydroxyvitamin D levels in serum at the onset of multiple sclerosis. Mult Scler 2005; 11(3):266-71.
  21. Shaygannejad V, Golabchi K, Haghighi S, Dehghan H, Moshayedi A. A comparative study of 25 (OH) vitamin D serum levels in patients with multiple sclerosis and control group in Isfahan, Iran. Int J Prev Med 2010; 1(3):195-201.
  22. Hatamian H, Bidabadi E, Seyed Saadat SM, Saadat NS, Kazemnezhad E, Ramezani H, et al. Is serum vitamin D levels associated with disability in patients with newly diagnosed multiple sclerosis? Iran J Neurol 2013; 12(2):41-6.
  23. Niino M, Sato S, Fukazawa T, Masaki K, Miyazaki Y, Matsuse D, et al. Decreased serum vitamin D levels in Japanese patients with multiple sclerosis. J Neuroimmunol 2015; 279:40-5.
  24. Hartl C, Obermeier V, Gerdes LA, Brügel M, von Kries R, Kümpfel T. Seasonal variations of 25-OH vitamin D serum levels are associated with clinical disease activity in multiple sclerosis patients. J Neurol Sci 2017; 375:160-4.
  25. Harandi AA, Shahbeigi S, Pakdaman H, Fereshtehnejad SM, Nikravesh E, Jalilzadeh R. Association of serum 25(OH) vitamin D3 concentration with severity of multiple sclerosis. Iran J Neurol 2012; 11(2):54-8.
  26. Royal W 3rd, Mia Y, Li H, Naunton K. Peripheral blood regulatory T cell measurements correlate with serum vitamin D levels in patientswith multiple sclerosis. J Neuroimmunol 2009; 213(1-2):135-41.
  27. Mominzadeh M, Mirzaii-Dizgah I, Mirzaii-Dizgah MR, Mirzaii-Dizgah MH. Stimulated saliva aminotransaminase alteration after experiencing acute hypoxia training. Air Med J 2014; 33(4):157-60.
  28. Mirzaii-Dizgah MH, Mirzaii-Dizgah MR, Mirzaii-Dizgah I. Serum and saliva total tau protein as a marker for relapsing-remitting multiple sclerosis. Med Hypotheses 2019; 135:109476.
  29. Mirzaii-Dizgah I, Riahi E. Salivary high-sensitivity cardiac troponin T levels in patients with acute myocardial infarction. Oral Dis 2013; 19(2):180-4.
  30. Mirzaii-Dizgah I, Riahi E. Serum and saliva levels of cathepsin L in patients with acute coronary syndrome. J Contemp Dent Pract 2011; 12(2):114-9.
  31. Fairney A, Saphier PW. Studies on the measurement of 25-hydroxy vitamin D in human saliva. Br J Nutr 1987; 57(1):13-25.


Journal of Kerman University of Medical Sciences
Volume 27, Issue 2
March and April 2020
Pages 106-112

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