Journal of Kerman University of Medical Sciences

The Effect of Eight Weeks of Aquatic Aerobic Training on ABCA1 and ABCG1 Genes Expression in the Blood Mononuclear Cells in Women After Coronary Artery Bypass Grafting

Document Type : Original Article

Authors

Mahdiyeh Haj Hosseini 1
Amir Rashidlamir 2
Mahtab Moazzami 2
Mansoor Moazenzadeh 3

1 PhD Student in Sports Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

2 Associate Professor in Sports Physiology Department, Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

3 Associate Professor, Cardiovascular Research Center, Kerman University of Medical Sciences, Kerman, Iran

https://doi.org/10.22062/jkmu.2020.90638
Abstract
Background: The aim of this research was to investigate the effect of aquatic aerobic training on regulatory factors related to Reverse Cholesterol Transport in women after coronary artery bypass grafting (CABG).
Methods: 24 middle-aged women were studied after coronary artery bypass grafting (12 were in control group and 12 in aquatic aerobic training group). The aquatic aerobic training program was performed in a pool of 1.20 m depth for eight weeks (three sessions per week with 50-75% intensity of the maximum heart rate). Furthermore, 48 hours before initiating the training program as well as 48 hours after the last training session, blood samples were taken in a fasting state. Then, Leukocytes were isolated, total cellular RNAs were extracted and complementary DNAs were synthesized. Gene expressions of ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette sub-family G member 1(ABCG1) were evaluated at messenger RNA levels using real-time PCR method. The amounts of ApolipoproteinA-1(Apo A-1), high-density lipoprotein cholesterol (HDL) and low-density lipoprotein cholesterol (LDL) were measured in plasma using an enzyme-linked immune sorbent assay method. Statistical analysis was performed using an independent-sample t-test and covariance, with a significance level accepted at P < 0.05.
Results: The findings showed that aquatic training was able to express ABCA1 and ABCG1 gene in women after coronary artery bypass grafting.
Conclusion: The data pointed to the possibility that aquatic training during the cardiac rehabilitation period can improve the reverse cholesterol transport and can be an alternative exercise program to achieve physical preparation and rehabilitation objectives in individuals who may have trouble doing exercises on the ground.

Keywords

Reverse Cholesterol Transport
Training
CABG
ABCA1
ABCG1
  1. Amiri F, Lotfi MH, Fallahzadeh H, Forouzannia SK. The association between lifestyle-related risk factors and coronary artery disease in residents of Yazd province: a case-control study. Health and Development Journal 2016; 5(2):98-109.[In Persian].
  2. Paynter NP, Balasubramanian R, Giulianini F, Wang DD, Tinker LF, Gopal S, et al. Metabolic predictors of incident coronary heart disease in women. Circulation 2018; 137(8):841-53.
  3. Karimi Zarchi AA, Naghiei MR. Prevalence of risk factors of coronary heart disease and effect of life-style modification guides. Kowsar Medical Journal 2009; 14(3):157-62. [In Persian].
  4. Kosmas CE, Martinez I, Sourlas A, Bouza KV, Campos FN, Torres V, et al. High-density lipoprotein (HDL) functionality and its relevance to atherosclerotic cardiovascular disease. Drugs Context; 2018: 7:212525.
  5. Schumacher T, Benndorf RA. ABC transport proteins in cardiovascular disease-A brief summary. Molecules 2017; 22(4):589.
  6. Rafieian-Kopaei M, Setorki M, Doudi M, Baradaran A, Nasri H. Atherosclerosis: process, indicators, risk factors and new hopes. Int J Prev Med 2014; 5(8):927-46.
  7. Milasan A, Jean G, Dallaire F, Tardif JC, Merhi Y, Sorci‐Thomas M, et al. Apolipoprotein A‐I modulates atherosclerosis through lymphatic vessel‐dependent mechanisms in mice. J Am Heart Assoc 2017; 6(9):e006892.
  8. Tofighi A. The effect of twelve weeks of aerobic training on ABCG1 gene expression in inactive obese women. Journal of Sport in Biomotor Sciences 2013; 5(10):62-70. [In Persian].
  9. Treat-Jacobson DJ, Lindquist R. Exercise, quality of life, and symptoms in men and women five to six years after coronary artery bypass graft surgery. Heart Lung 2007; 36(6):387-97.
  10. Eagle KA, Guyton RA, Davidoff R, Edwards FH, Ewy GA, Gardner TJ, et al. ACC/AHA 2004 guideline update for coronary artery bypass graft surgery: summary article. a report of the American College of Cardiology/American Heart Association task force on practice Guidelines (Committee to Update the 1999 guidelines for coronary artery bypass graft surgery). J Am Coll Cardiol 2004; 44(5):e213-310.
  11. Moazzami Z, Rahimi T, Tarbiat M, Soltanian A. Stages of physical activity in patients after coronary artery bypass graft surgery: application of trans-theoretical model. Health Education and Health Promotion (HEHP) 2016; 4(3):23-36.
  12. Moholdt TT, Amundsen BH, Rustad LA, Wahba A, Løvø KT, Gullikstad LR, et al. Aerobic interval training versus continuous moderate exercise after coronary artery bypass surgery: a randomized study of cardiovascular effects and quality of life. Am Heart J 2009; 158(6):1031-7.
  13. Adsett JJ, Morris N, Kuys S, Hwang R, Mullins R, Khatun M, et al. Aquatic exercise training is effective in maintaining exercise performance in trained heart failure patients: a randomised crossover pilot trial. Heart Lung Circ 2016; 26(6):572-9.
  14. Abedi HA, Bik Mohammadi S, Abdeyazdan GH, Nazari H. A survey on the the lifestyle of the heart desease patients after discharge from hospital in urmia seyyed alshohada in 1391. Nursing and Midwifery Journal 2014; 11(12):944-54. [In Persian].
  15. Hajighasemi A, Ravasi A, Kordi M, Rashidlamir A, Ghorghi A. Investigation of the effect of cardiac rehabilitation program on peripheral blood mononuclear cells ABCA1 gene expression in myocardial infractions patient. Journal of Knowledge & Health 2017; 11(4):23-9. [In Persian].
  16. Zeiaadini Dashtkhaki L, Rashid Lamir A, Naghibi S. The effect of aquatic and dryland resistance training on peroxisome proliferator activated receptor-ɑ gene expression in middle-aged women’s peripheral blood mononuclear cell after coronary artery bypass grafting. Annals of Applied Sport Science 2017; 5(4):13-22.
  17. Khajei R, Haghighi AH, Hamedinia M, Rashid Lamir A. The effect of eight weeks of aerobic exercise on the expression of liver monocyte X-receptor gene value and lipid profile of middle-aged men after cardiac artery bypass graft surgery. Journal of Sabzevar University of Medical Sciences 2017; 25(4):507-17. [In Persian].
  18. Volaklis KA, Smilios I, Spassis AT, Zois CE, Douda HT, Halle M, et al. Acute pro-and anti-inflammatory responses to resistance exercise in patients with coronary artery disease: a pilot study. J Sports Sci Med 2015; 14(1):91-7.
  19. Gaieni A. Comparison of eight weeks of combined and aerobic training on functional capacity, body composition and strength in post-coronary artery bypass graft cardiac patients. Iranian Journal Of Cardiovascular Nursing 2013; 2(1):34-41.
  20. Dalir Z, Vahdat Feizabadi E, Mazlom S, Rajaee Khorasani A. The Effect of short-term cardiac rehabilitation program on anxiety and depression in patients after coronary artery bypass surgery. Evidence Based Care 2013; 3(3):33-42. [In Persian].
  21. Fallahi AA, Gaeini AA, Kordi MR, Nejatian M, Etemadi S. The effects of continuous and interval exercise training on quality of life in heart patients after coronary artery bypass graft surgery (CABG). Sport Biosciences (Harakat) 2011.
  22. Dor-Haim H, Barak S, Horowitz M, Yaakobi E, Katzburg S, Swissa M, et al. Improvement in cardiac dysfunction with a novel circuit training method combining simultaneous aerobic-resistance exercises. A randomized trial. PloS One 2018; 13(1):e0188551.
  23. Kachur S, Chongthammakun V, Lavie CJ, De Schutter A, Arena R, Milani RV, et al. Impact of cardiac rehabilitation and exercise training programs in coronary heart disease. Prog Cardiovasc Dis 2017; 60(1):103-14.
  24. Ayanian JZ, Guadagnoli E, Cleary PD. Physical and psychosocial functioning of women and men after coronary artery bypass surgery. JAMA 1995; 274(22):1767-70.
  25. Covinsky KE, Lin F, Bittner V, Hlatky MA, Knight SJ, Vittinghoff E. Health-related quality of life following coronary artery bypass graft surgery in post-menopausal women. J Gen Intern Med 2008; 23(9):1429-34.
  26. Rosenson RS, Brewer Jr HB, Davidson WS, Fayad ZA, Fuster V, Goldstein J, et al. Cholesterol efflux and atheroprotection: advancing the concept of reverse cholesterol transport. Circulation 2012; 125(15):1905-19.
  27. Vaccarino V, Parsons L, Every NR, Barron HV, Krumholz HM. Sex-based differences in early mortality after myocardial infarction. N Engl J Med 1999; 341(4):217-25.
  28. Bergamin M, Ermolao A, Tolomio S, Berton L, Sergi G, Zaccaria M. Water-versus land-based exercise in elderly subjects: effects on physical performance and body composition. Clin Interv Aging 2013; 8:1109-17.
  29. Volaklis KA, Spassis AT, Tokmakidis SP. Land versus water exercise in patients with coronary artery disease: effects on body composition, blood lipids, and physical fitness. Am Heart J 2007; 154(3):560. e1-6.
  30. American College of Sports Medicine, Chodzko-Zajko WJ, Proctor DN, Singh MA, Minson CT, Nigg CR, et al. American College of Sports Medicine position stand. Exercise and physical activity for older adults. Med Sci Sports Exerc 2009; 41(7):1510-30.
  31. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 2001; 25(4):402-8.
  32. Marques LR, Diniz TA, Antunes BM, Rossi FE, Caperuto EC, Lira FS, et al. Reverse cholesterol transport: Molecular mechanisms and the non-medical approach to enhance HDL cholesterol. Front Physiol 2018; 9:526.
  33. Milasan A, Ledoux J, Martel C. Lymphatic network in atherosclerosis: the underestimated path. Future Sci OA 2015; 1(4): FSO61.
  34. Milasan A, Dallaire F, Mayer G, Martel C. Effects of LDL receptor modulation on lymphatic function. Sci Rep 2016; 6:27862.
  35. Martel C, Randolph GJ. Atherosclerosis and transit of HDL through the lymphatic vasculature. Curr Atheroscler Rep 2013; 15(9):354.
  36. Martel C, Li W, Fulp B, Platt AM, Gautier EL, Westerterp M, et al. Lymphatic vasculature mediates macrophage reverse cholesterol transport in mice. J Clin Invest 2013; 123(4):1571-9.
  37. Vuorio T, Nurmi H, Moulton K, Kurkipuro J, Robciuc MR, Öhman M, et al. Lymphatic vessel insufficiency in hypercholesterolemic mice alters lipoprotein levels and promotes atherogenesis. Arterioscler Thromb Vasc Biol 2014; 34(6):1162-70.
  38. Tofighi A, Rahmani F, Qarakhanlou BJ, Babaei S. The effect of regular aerobic exercise on reverse cholesterol transport A1 and apo lipoprotein aI gene expression in inactive women. Iran Red Crescent Med J 2015; 17(4):e26321.
  39. Jafari M, Rashidlamir A, Dastani M, Fathi M, Alavinya SE. The effect of cardiac rehabilitation on ApoA1 and ApoB in men with coronary heart disease (CHD) after coronary artery bypass graft (CABG). Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch 2018; 28(2):117-23. [In Persian].
  40. Rashidlamir A, Dastani M, Saadatnia A, Bassami MR. Effect of cardiac rehabilitation training on ABCA1 expression in lymphocytes of patients undergoing coronary artery bypass graft operation. Zahedan Journal of Research in Medical Sciences 2018; In Press(In Press):e11277.
  41. Koba S, Ayaori M, Uto-Kondo H, Furuyama F, Yokota Y, Tsunoda F, et al. Beneficial effects of exercise-based cardiac rehabilitation on high-density lipoprotein-mediated cholesterol efflux capacity in patients with acute coronary syndrome. J Atheroscler Thromb 2016; 23(7):865-77.
  42. Balady GJ, Williams MA, Ades PA, Bittner V, Comoss P, Foody JM, et al. Core components of cardiac rehabilitation/secondary prevention programs: 2007 update: a scientific statement from the american heart association exercise, cardiac rehabilitation, and prevention committee, the council on clinical cardiology; the councils on cardiovascular nursing, epidemiology and prevention, and nutrition, physical activity, and metabolism; and the american association of cardiovascular and pulmonary rehabilitation. Circulation 2007; 115(20):2675-82.
  43. Warburton DE, Katzmarzyk PT, Rhodes RE, Shephard RJ. Evidence-based guidelines for physical activity of adult Canadians. Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition et Metabolisme 2007; 32(Suppl 2):S17-74.
  44. Rahmati-Ahmadabad S, Shirvani H, Ghanbari-Niaki A, Rostamkhani F. The effects of high-intensity interval training on reverse cholesterol transport elements: a way of cardiovascular protection against atherosclerosis. Life Sci 2018; 209:377-82.
  45. Kazeminasab F, Marandi M, Ghaedi K, Esfarjani F, Moshtaghian J. Effects of a 4-week aerobic exercise on lipid profile and expression of LXRα in rat liver. Cell J 2017; 19(1):45-9.
  46. O'Donovan G, Owen A, Bird SR, Kearney EM, Nevill AM, Jones DW, et al. Changes in cardiorespiratory fitness and coronary heart disease risk factors following 24 wk of moderate-or high-intensity exercise of equal energy cost. J Appl Physiol (1985( 2005; 98(5):1619-25.
  47. Sorci-Thomas MG, Thomas MJ. Microdomains, inflammation, and atherosclerosis. Circ Res 2016; 118(4):679-91.
  48. Kontush A, Lindahl M, Lhomme M, Calabresi L, Chapman MJ, Davidson WS. Structure of HDL: particle subclasses and molecular components. Handb Exp Pharmacol 2015; 224:3-51.
  49. Hutchins PM, Heinecke JW. Cholesterol efflux capacity, macrophage reverse cholesterol transport, and cardioprotective HDL. Curr Opin Lipidol 2015; 26(5):388-93.
  50. Srivastava N. ATP binding cassette transporter A1-key roles in cellular lipid transport and atherosclerosis. Mol Cell Biochem 2002; 237(1-2):155-64.
  51. Ghanbari-Niaki A, Ghanbari-Abarghooi S, Rahbarizadeh F, Zare-Kookandeh N, Gholizadeh M, Roudbari F, et al. Heart ABCA1 and PPAR-α genes expression responses in male rats: effects of high intensity treadmill running training and aqueous extraction of black crataegus-pentaegyna. Res Cardiovasc Med 2013; 2(4):153-9.
  52. Ghanbari Niaki A, Ghanbari Abarghooi S, Gholizadeh M. Heart ATP-binding cassette protein A1 and G1, peroxisome proliferator-activated receptor-α and liver X receptors genes expression in response to intensive treadmill running and red crataegus pentaegyna (Sorkh valik) in male rats. Zahedan Journal of Research in Medical Sciences 2015; 17(5):1-5.
  53. Chen VC, Lee CW, Bui S, Lee T, Fluckey J, Riechman S. Skeletal muscle PPAR-delta increases with 10 weeks of resistance training (LB819). The FASEB Journal 2014;28 (1_supplement):LB819.
  54. Rashidlamir A. Investigation of the effect of aerobic and resistance exercises on peripheral blood mononuclear cells ABCG1 gene expression in female athletes. Journal of Shahid Sadoughi University of Medical Sciences 2012; 20(1):1-9. [In Persian].
  55. Abadi FH, Elumalai G, Sankaraval M, Ramli FA. Effects of aqua-aerobic exercise on the cardiovascular fitness and weight loss among obese students. Int J Physiother 2017; 4(5):278-83.
  56. Singh MP, Pathak D, Sharma GK, Sharma CS. Peroxisome proliferator-activated receptors (PPARS): a target with a broad therapeutic potential for human diseases: an overview. Pharmacologyonline 2011; 2:58-89.

 

 

 

Journal of Kerman University of Medical Sciences
Volume 27, Issue 3
May and June 2020
Pages 190-203

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