Journal of Kerman University of Medical Sciences

The Effects of Hydro-alcoholic Extracts of Allium sativum L. and Orchismaculata L. on Spermatogenesis Index and Testosterone Level in Cyclophosphamide-treated Rats

Document Type : Original Article

Authors

Firouze Sadeghzadeh 1
Azizieh Sadeghzadeh 1
Saeed Changizi-Ashtiyani 2
Abass Alimoradian 1
Mehry Mashayekhei 3
Ali Zarei 4
Farideh Jalali-Mashayekhi 5

1 Traditional and Complementary Medicine Research Center, Arak University of Medical University, Arak, Iran

2 Professor, Department of Physiology, School of Paramedical Sciences, Arak University of Medical Sciences, Arak, Iran

3 Assistant Professor, Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute, Tehran, Iran

4 Assistant Professor, Department of Physiology, Shiraz University of Medical University, Shiraz, Iran

5 Assistant Professor, Traditional and Complementary Medicine Research Center, Arak University of Medical University, Arak, Iran

https://doi.org/10.22062/jkmu.2020.90642
Abstract
Background:One of the most common side effects of cyclophosphamide (CP) is oligo-spermatozoa, azoospermia and elimination of spermatogenic cycles. This study was done to find the efficacy of simultaneous consumption of garlic (Allium sativum L.) and orchid (Orchismaculata L.) hydroalcoholic extracts on spermatogenesis and sex hormones in rats treated with CP.
Methods:Forty-two male rats were divided into the six groups: control, sham, CP
(5 mg/kg), garlic (10 mg/kg) + CP, orchid (40 mg/kg) + CP, garlic+ orchid + CP. All of the agents were administered through oral gavage for 28 days.
Results: The number of sperms increased in garlic+ CP and orchid+ CP groups. The percentage of sperm forward motility increased in groups receiving garlic, orchid, and garlic +orchid compared with the group that received only CP.
Antioxidant total capacity and testosterone level showed significant increases in garlic+ orchid, garlic and orchid groups respectively compared with the group that received only CP (p <0.05). Also, the tissue and serum malondialdehyde levels reduced in the group received garlic +orchid compared with the group that received only CP.
Conclusion:Garlic and orchid could increase the number and the motility of sperms, index of sertoli cell, antioxidant capacity and serum testosterone level.

Keywords

Infertility
Garlic
Orchid
Cyclophosphamide
Spermatogenesis
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Journal of Kerman University of Medical Sciences
Volume 27, Issue 3
May and June 2020
Pages 232-243

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