Journal of Kerman University of Medical Sciences

Frequency of Human Papillomavirus Genotypes in Head and Neck Epithelial Cancers in the Ear, Throat, and Nose Department of Shafa Hospital, Kerman, Iran in 2017

Document Type : Original Article

Authors

Soolmaz Ghorbani 1
Hamidreza Mollaei 2
Tourajreza Mirshekari 3
Habibeh Ahmadipour 4
Ali asghar Arabi mianroodi 1
Kimia Iranmanesh 1
Narges Khanjani 5

1 Department of Otorhinolaryngology, Head and Neck Surgery, Kerman University of Medical Sciences, Kerman, Iran

2 Department of Medical Microbiology, Kerman University of Medical Sciences, Kerman, Iran

3 Department of Pathology, Kerman University of Medical Sciences, Kerman, Iran

4 Department of Social Medicine, Kerman University of Medical Sciences, Kerman, Iran

5 Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran

https://doi.org/10.22062/jkmu.2021.91719
Abstract
Background: Human papillomavirus virus (HPV) plays an important role in some human malignancies, including head and neck squamous cell carcinoma (SCC). However, it is necessary to evaluate the role of carcinogenic agents in local settings as well. Therefore, this study aimed to evaluate the frequency of HPV genotypes in head and neck epithelial cancers.
Methods: In this case-control study, 202 patients with suspected carcinoma referred to Shafa Hospital (Kerman, Iran, 2017) for pathological evaluation were enrolled. A definitive diagnosis of carcinoma was reported for 101 patients (case group) and in the other 101 subjects, carcinoma was not diagnosed. In order to determine the presence and type of HPV in tissue samples, the PCR method was used. Data analysis was performed using Fisher’s exact test, independent t-test, and logistic regression. 
Results: In total, 27 head and neck SCC samples (26.7%) were positive for HPV DNA. In addition, the virus species were HPV-11 in 15 cases (14.9%), HPV-16 and HPV-18 in 12 cases (11.9%), and HPV-6 in 10 cases (9.9%). According to the results, the presence of HPV was significantly higher in patients with SCC (P < 0.001). Moreover, smoking (P = 0.03) and opioid use (P < 0.001) were also significantly associated with SCC.
Conclusion: The results of the present study demonstrated the presence of HPV in tissue samples of head and neck SCC patients in Kerman, Iran. Further studies are needed to investigate the preventive role of HPV vaccination in head and neck SCC.

Keywords

HPV
Head and Neck Cancers
Kerman
  1. Ragin CC, Modugno F, Gollin SM. The epidemiology and risk factors of head and neck cancer: a focus on human papillomavirus. J Dent Res 2007; 86(2):104-14. doi: 10.1177/154405910708600202.
  2. Global Burden of Disease Cancer Collaboration, Fitzmaurice C, Allen C, Barber RM, Barregard L, Bhutta ZA, et al. Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: A Systematic Analysis for the Global Burden of Disease Study. JAMA Oncol 2017; 3(4):524-48. doi: 10.1001/jamaoncol.2016.5688.
  3. Koyanagi YN, Matsuo K, Ito H, Wakai K, Nagata C, Nakayama T, et al. Cigarette smoking and the risk of head and neck cancer in the Japanese population: a systematic review and meta-analysis. Jpn J Clin Oncol 2016; 46(6):580-95. doi: 10.1093/jjco/hyw027.
  4. Kawakita D, Matsuo K. Alcohol and head and neck cancer. Cancer Metastasis Rev 2017; 36(3):425-34. doi: 10.1007/s10555-017-9690-0.
  5. Nikakhlagh S, Saki N, Shoar MH, Sartipipor A, Saki S. Incidence of etiologic factors in squamous cell carcinoma of head and neck in ahvaz. Iran J Otorhinolaryngol 2012; 24(67):85-90.
  6. Prabhu SR, Wilson DF. Evidence of Epstein–Barr virus association with head and neck cancers: a review. J Can Dent Assoc 2016; 82:g2.
  7. Bishop JA, Guo TW, Smith DF, Wang H, Ogawa T, Pai SI, et al. Human papillomavirus-related carcinomas of the sinonasal tract. Am J Surg Pathol 2013; 37(2):185-92. doi: 10.1097/PAS.0b013e3182698673.
  8. Nikakhlagh S, Saki N, Mokvandi M, Jahanshahi J, Emadmostofi N. Detection and typing of human papilloma virus DNA by PCR in head and neck squamous cell carcinoma in E.N.T. Ward of Ahwaz Imam Hospital. Avicenna Journal of Clinical Medicine 2008; 15(2):19-22. [In Persian].
  9. Li W, Thompson CH, Xin D, Cossart YE, O'Brien CJ, McNeil EB, et al. Absence of human papillomavirus in tonsillar squamous cell carcinomas from Chinese patients. Am J Pathol 2003; 163(6):2185-9. doi: 10.1016/S0002-9440(10)63576-6.
  10. Kaya H, Kotiloğlu E, Inanli S, Ekicioğlu G, Bozkurt SU, Tutkun A, et al. Prevalence of human papillomavirus (HPV) DNA in larynx and lung carcinomas. Pathologica 2001; 93(5):531-4.
  11. Sohrabi A, Rahnamaye Farzami M, Mirab Samiee S, Modares MH. An overview on papillomaviruses as the main cause of cervical cancer. Iranian Journal of Obstetrics, Gynecology and Infertility 2015; 18(145):14-25. [In Persian].
  12. Onsory K, Ahmadi AR, Jalilvand E. Epidemiology of human papilloma virus (HPV) type 16 and 18 in the patients with cervical cancer in Tehran. Journal of Microbial World 2016; 8(4):281-9. [In Persian].
  13. Tomaić V. Functional roles of E6 and E7 oncoproteins in HPV-Induced malignancies at diverse anatomical sites. Cancers (Basel) 2016; 8(10):95. doi: 10.3390/cancers8100095.
  14. Fakhraei F, Haghshenas MR. Human papillomaviruses and cancer. Journal of Mazandaran University of Medical Sciences 2013; 23(98):340-60. [In Persian].
  15. Spence T, Bruce J, Yip KW, Liu FF. HPV associated head and neck cancer. Cancers (Basel) 2016; 8(8):75. doi: 10.3390/cancers8080075.
  16. D'Souza G, Dempsey A. The role of HPV in head and neck cancer and review of the HPV vaccine. Prev Med 2011; 53(Suppl 1):S5-S11. doi: 10.1016/j.ypmed.2011.08.001.  
  17. Johnson ME, Cantalupo PG, Pipas JM. Identification of head and neck cancer subtypes based on human papillomavirus presence and E2F-Regulated gene expression. mSphere 2018; 3(1):e00580-17. doi: 10.1128/mSphere.00580-17.
  18. Torrente MC, Rodrigo JP, Haigentz Jr M, Dikkers FG, Rinaldo A, Takes RP, et al. Human papillomavirus infections in laryngeal cancer. Head Neck 2011; 33(4):581-6. doi: 10.1002/hed.21421.
  19. Hernandez BY, Goodman MT, Lynch CF, Cozen W, Unger ER, Steinau M, et al. Human papillomavirus prevalence in invasive laryngeal cancer in the United States. PLoS One 2014; 9(12):e115931. doi: 10.1371/journal.pone.0115931.  
  20. Flint PW, Haughey BH, Robbins KT, Thomas JR, Niparko JK, Lund VJ, et al. 7th ed. Cummings Otolaryngology E-Book: Head and Neck Surgery. Elsevier; 2020.
  21. Zarei MR, Moradi A, Chamani G, Kakouei S, Hamkar R, Mohammad AS, et al. Detection of human papillomavirus DNA sequences in oral lesions using polymerase chain reaction. Acta Medica Iranica. 2007; 5(3):177-182.
  22. Afshar RM, Mollaie HR, Fazlalipour M, Arabzadeh SA. Prevalence and type distribution of human papillomavirus infection using the INNo-Lipa assay, Kerman, Southeast Iran. Asian Pac J Cancer Prev 2013; 14(9):5287-91. doi: 10.7314/apjcp.2013.14.9.5287.
  23. Ghasemian E, Monavari SH, Irajian GR, Jalali Nodoshan MR, Roudsari RV, Yahyapour Y. Evaluation of human papillomavirus infections in prostatic disease: a cross-sectional study in Iran. Asian Pac J Cancer Prev 2013; 14(5):3305-8. doi: 10.7314/apjcp.2013.14.5.3305.
  24. Eftekhaar NS, Karbalaie Niya MH, Izadi F, Teaghinezhad-S S, Keyvani H. Human papillomavirus (HPV) genotype distribution in patients with recurrent respiratory papillomatosis (RRP) in Iran. Asian Pac J Cancer Prev 2017; 18(7):1973-6. doi: 10.22034/APJCP.2017.18.7.1973.
  25. Yahyapour Y, Shamsi-Shahrabadi M, Mahmoudi M, Siadati S, Shahryar SS, Shokri-Shirvani J, et al. Evaluation of human papilloma virus infection in patients with esophageal squamous cell carcinoma from the Caspian Sea area, north of Iran. Asian Pac J Cancer Prev 2012; 13(4):1261-6. doi: 10.7314/apjcp.2012.13.4.1261.
  26. Karbalaie Niya MH, Safarnezhad Tameshkel F, Panahi M, Bokharaei Salim F, Monavari SH, Keyvani H. Human papillomavirus investigation in head and neck squamous cell carcinoma: initial report from the low risk HPV types associations. Asian Pac J Cancer Prev 2017; 18(9):2573-9. doi: 10.22034/APJCP.2017.18.9.2573.
  27. Furuta Y, Takasu T, Asai T, Shinohara T, Sawa H, Nagashima K, et al. Detection of human papillomavirus DNA in carcinomas of the nasal cavities and paranasal sinuses by polymerase chain reaction. Cancer 1992; 69(2):353-7. doi: 10.1002/1097-0142(19920115)69:23.0.co;2-j.
  28. Correnti M, Rivera H, Cavazza ME. Detection of human papillomaviruses of high oncogenic potential in oral squamous cell carcinoma in a Venezuelan population. Oral Dis 2004; 10(3):163-6. doi: 10.1046/j.1601-0825.2003.00989.x.
  29. Li X, Gao L, Li H, Gao J, Yang Y, Zhou F, et al. Human papillomavirus infection and laryngeal cancer risk: a systematic review and meta-analysis. J Infect Dis 2013; 207(3):479-88. doi: 10.1093/infdis/jis698.
  30. Yang D, Shi Y, Tang Y, Yin H, Guo Y, Wen S, et al. Effect of HPV infection on the occurrence and development of laryngeal cancer: a review. J Cancer 2019; 10(19):4455-62. doi: 10.7150/jca.34016.
  31. Ragin CC, Taioli E. Survival of squamous cell carcinoma of the head and neck in relation to human papillomavirus infection: review and meta‐analysis. Int J Cancer 2007; 121(8):1813-20. doi: 10.1002/ijc.22851.
  32. Reimers N, Kasper HU, Weissenborn SJ, Stützer H, Preuss SF, Hoffmann TK, et al. Combined analysis of HPV‐DNA, p16 and EGFR expression to predict prognosis in oropharyngeal cancer. Int J Cancer 2007; 120(8):1731-8. doi: 10.1002/ijc.22355.
  33. Na I, Kang HJ, Cho SY, Koh JS, Lee JK, Lee BC, et al. EGFR mutations and human papillomavirus in squamous cell carcinoma of tongue and tonsil. EJC 2006; 43(3):520-26.
  34. Sugiyama M, Bhawal UK, Kawamura M, Ishioka Y, Shigeishi H, Higashikawa K, et al. Human papillomavirus-16 in oral squamous cell carcinoma: clinical correlates and 5-year survival. Br J Oral Maxillofac Surg 2007; 45(2):116-22. doi: 10.1016/j.bjoms.2006.04.012.  
  35. Licitra L, Perrone F, Bossi P, Suardi S, Mariani L, Artusi R, et al. High-risk human papillomavirus affects prognosis in patients with surgically treated oropharyngeal squamous cell carcinoma. J Clin Oncol 2006; 24(36):5630-6. doi: 10.1200/JCO.2005.04.6136.
  36. Weinberger PM, Yu Z, Haffty BG, Kowalski D, Harigopal M, Brandsma J, et al. Molecular classification identifies a subset of human papillomavirus-associated oropharyngeal cancers with favorable prognosis. J Clin Oncol 2006; 24(5):736-47. doi: 10.1200/JCO.2004.00.3335.
  37. Báez A, Almodovar JI, Cantor A, Celestin F, Cruz‐Cruz L, Fonseca S, et al. High frequency of HPV16‐associated head and neck squamous cell carcinoma in the Puerto Rican population. Head Neck 2004; 26(9):778-84. doi: 10.1002/hed.20046.
  38. Ringström E, Peters E, Hasegawa M, Posner M, Liu M, Kelsey KT. Human papillomavirus type 16 and squamous cell carcinoma of the head and neck. Clin cancer Res 2002; 8(10):3187-92.
  39. Mellin H, Dahlgren L, Munck‐Wikland E, Lindholm J, Rabbani H, Kalantari M, et al. Human papillomavirus type 16 is episomal and a high viral load may be correlated to better prognosis in tonsillar cancer. Int J Cancer 2002; 102(2):152-8. doi: 10.1002/ijc.10669.
  40. Harlé, A., Guillet, J., Thomas, J. Sastre-Garau X,Rouyer M,Ramacci c,et al. Evaluation and validation of HPV real-time PCR assay for the detection of HPV DNA in oral cytobrush and FFPE samples. Sci Rep  2018; 11313(8). .doi.org/10.1038/s41598-2018-29790-z.
  41. Economopoulou P, Kotsantis I, Psyrri A. Special issue about head and neck cancers: HPV positive cancers. Int J Mol Sci 2020; 21(9):3388. doi: 10.3390/ijms21093388.
  42. Augustin JG, Lepine C, Morini A, Brunet A, Veyer D, Brochard C, et al. HPV detection in head and neck squamous cell carcinomas: what is the issue? Front Oncol 2020; s10:1751. doi: 10.3389/fonc.2020.01751.
  43. Monsfi N, Dabiri SH, Abbaszadeh M, Safizadeh H, Fotouhi-Ardakani R, et al. Frequency of Dysplastic and Cancerous Pap Smear and Genotyping of Human Papillomavirus by DNA Probetechniques in Kerman, Iran. Journal of Kerman University of Medical Sciences, 2013; 20(5): 450-459.
  44. Jafari E, Dabiri SH, Mortazaeizadeh A, Sayadi AR, Amir Poor Rostami S. Non- Detection of HPV DNA in Prostatic Cancer and Benign Prostatic Hyperplasia: a case- control study in Kerman. Journal of Kerman University of Medical Sciences, 2018; 25(1): 77-83.

 

 

Journal of Kerman University of Medical Sciences
Volume 28, Issue 4
July and August 2021
Pages 382-390

Home

Editorial Board

Guide for Authors

Submit Manuscript

Guide for Reviewers

Contact Us