Journal of Kerman University of Medical Sciences

Antibiotic Resistance Pattern and Molecular Typing by PCR-RAPD Analysis in Clinical Isolates of Pseudomonas aeruginosa from Motahari Hospital, Tehran, Iran

Document Type : Short Communication

Authors

Mahmood Barati 1
Mazaher Gholipourmalekabadi 2
Javad Ranjbari 3
Pegah Shakib 4
Mohammad Ali Bahar 5
Ali Samadikuchaksaraei 1

1 Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

2 Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

3 Department of Biotechnology, Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

5 Department of Immunology and Burn Research Center, Iran University of Medical Sciences, Tehran, Iran

https://doi.org/10.22062/jkmu.2021.91725
Abstract
Background: Pseudomonas aeruginosa infection is one of the major challenges in burn patients. This organism is resistant to a wide range of antibiotics. The aim of this study was to investigate the antibiotic susceptibility and genetic relatedness in Pseudomonas aeruginosa isolated from patients admitted to Motahari Burn Center, Tehran, Iran.
Methods: This study was conducted on 186 burn patients with Pseudomonas aeruginosa colonization following admission in Motahari Burn Center during one year. Antibiotic resistance test was performed by disc diffusion method and genetic relatedness was evaluated by PCR-RAPD analysis.
Results: The highest and lowest percentage of resistance was observed against ceftizoxime and ciprofloxacin. The genotyping study by RAPD PCR technique revealed 57 different genotypes, among which RAPD 5, RAPD 8, and RAPD 9 were the most prevalent patterns and produced by 14%, 9%, and 7% of the isolates, respectively.
Conclusion: In total, no association was found between RAPD genotypes and antibiotic resistance patterns, and death rate.

Keywords

RAPD PCR
Pseudomonas aeruginosa
Antibiotic resistance
Genotypes
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Journal of Kerman University of Medical Sciences
Volume 28, Issue 4
July and August 2021
Pages 420-426

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