Can Excessive Consumption of Celery Threaten Continuation of pregnancy: a Biometrical and Histopathological Study of Mice Placenta

Document Type : Original Article

Authors

1 Medical Nanotechnology & Tissue Engineering Research center, Yazd Reproductive Science Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran & Research and Clinical Center for Infertility, Yazd Reproductive Science Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran & Department of Reproductive Biology and Anatomical Science, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Research and Clinical Center for Infertility, Yazd Reproductive Science Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran & Department of Reproductive Biology and Anatomical Science, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

4 Department of Reproductive Biology and Anatomical Science, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

5 Food and Drug Labrarory, Food and Drug Deputy, Shahid Sadoughi University of Medical Sciences

Abstract

Background: Placenta has a strategic role in fetal/maternal exchange. A healthy placenta is essential for normal fetal development. Avoiding consumption of some foods and vegetables is suggested during pregnancy due to the risk of placental disorders. Celery is a high consumed vegetable that some studies concern about its adverse effect on pregnancy. The present study focused on its consumption side effects in different trimesters of gestation on mouse placenta.
Methods: Duration of pregnancy in mice is three weeks. Five groups of 5 mice were considered. Groups I, II and III received celery hydro-alcoholic extract only during the 1st, 2nd and 3rd weeks of pregnancy, respectively and group IV received it during all the weeks of gestation. The 5th group was considered as control and just received placebo. The biometrical sizes of placenta (weight, length and diameter) were measured and histopathological analysis was done.
Results: Celery consumption decreased the weight of placenta in groups II, III and IV. Also, placental length and diameter decreased in groups I and IV. Histopathological examination showed decrease of placenta trophoblastic giant cells and increase of trophoblast glycogen cells in the basal layer. Generally, morphological and pathological changes in the 4th group showed more deviations from the control group. Basal and Labyrinth layer thickness decreased in the experimental groups. Also, hyperaemia was observed in labyrinth layer of the experimental groups.
Conclusion: Due to the decrease of placenta biometrical sizes and histo-pathological adverse effects, it is advised that celery should be used with more precaution, during pregnancy.

Keywords


  1. Baschat AA, Hecher K. Fetal growth restriction due to placental disease. Semin. Perinatol.; 2004;28(1):67-80.
  2. Morgan TK. Role of the placenta in preterm birth: a review. J. Perinatol. 2016;33(03):258-266. DOI: 10.1055/s-0035-1570379.
  3. Jauniaux E, Van Oppenraaij RH, Burton GJ. Obstetric outcome after early placental complications. Curr Opin Obstet Gynecol. 2010;22(6):452-457. DOI: 10.1097/GCO.0b013e3283404e44.
  4. Yang J, Wang Y, Wang X-y, Zhao Y-y, Wang J, Zhao Y-y. Adverse pregnancy outcomes of patients with history of first-trimester recurrent spontaneous abortion. Biomed Res. Int. 2017;2017.especial issue, 7 page. 10.1155/2017/4359424.
  5. Tewari V, Tewari A, Bhardwaj N. Histological and histochemical changes in placenta of diabetic pregnant females and its comparision with normal placenta. Asian Pac J Trop Dis. 2011;1(1):1-4.
  6. Bulucea CA, Mastorakis NE, Paun MF, Marcu R. Histopathological placental screening as valuable and non-invasive method for assessing etiology of second trimester recurrent abortion. Adv. Biomed. Res. 2010;180(349)23-25).
  7. Reijnders IF, Mulders AG, Koster MP. Placental development and function in women with a history of placenta‐related complications: a systematic review. Acta Obstet Gynecol Scand. 2018;97(3):248-257; 10.1111/aogs.13259.
  8. Hanita O, Hanisah A. Potential use of single measurement of serum progesterone in detecting early pregnancy failure. Malays J Pathol. 2012;34(1):41-6; 1186/2193-1801-1-80.
  9. Wu F, Tian FJ, Lin Y, Xu WM. Oxidative stress: placenta function and dysfunction. AM. J. Reprod. Immunol. 2016;76(4):258-271; 10.1111/aji.12454.
  10. Pathak S, Sebire NJ, Hook L, et al. Relationship between placental morphology and histological findings in an unselected population near term. Virchows Arch. 2011;459(1):11-20; 10.1007/s00428-011-1061-6.
  11. Ptacek I, Smith A, Garrod A, et al. Quantitative assessment of placental morphology may identify specific causes of stillbirth. BMC Clin. Pathol. 2016;16(1):1; 1186/s12907-016-0023-y.
  12. Yakubu M, Akanji M, Oladiji A. Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials. Pharmacogn. Rev. 2007;1(1):49.
  13. Giacosa A, Morazzoni P, Bombardelli E, Riva A, Bianchi Porro G, Rondanelli M. Can nausea and vomiting be treated with ginger extract. Eur Rev Med Pharmacol Sci. 2015;19(7):1291-6.
  14. Darvishpor S, Hosseini A, Davoodi A, Salehifar E, Akbari J, Azadbakht M. A review on medicinal plants used for nausea and vomiting in Persian Medicine. Global Journal Inc. 2018;18(1).
  15. Kooti W, Ali-Akbari S, Asadi-Samani M, Ghadery H, Ashtary-Larky D. A review on medicinal plant of Apium graveolens. Adv. Herb. Med. 2015;1(1):48-59.
  16. Tyagi S. Medical benefits of Apium graveolens (celery herb). Drug Discov. Ther. 2013;1(05).
  17. Kooti W, Ghasemiboroon M, Ahangarpoor A, et al. The effect of hydro-alcoholic extract of celery on male rats in fertility control and sex ratio of rat offspring. J of Babol University of Med Sci. 2014;16(4):43-49; 10.22038/ijogi.2018.12135.
  18. Al-Asmari AK, Athar MT, Kadasah SG. An updated phytopharmacological review on medicinal plant of Arab region: Apium graveolens linn. Pharmacogn. Rev. 2017;11(21):13.
  19. Kooti W, Ghasemiboroon M, Asadi-Samani M, et al. The effect of halcoholic extract of celery leaves on the delivery rate (fertilization and stillbirths), the number, weight and sex ratio of rat off spring. Adv. Environ. Biol. 2014;8(10):824-830.
  20. Kooti W, Moradi M, Peyro K, et al. The effect of celery (Apium graveolens L.) on fertility: A systematic review. J. Complement. Integr. Med. 2018;15(2).
  21. Ciganda C, Laborde A. Herbal infusions used for induced abortion. J. Toxicol. Clin. Toxicol. 2003;41(3):235-239.
  22. Bazafkan MH, Hardani A, Zadeh A, et al. The Effects of Aqueous Extract of Celery Leaves (Apium Gravelens) on the Delivery Rate, Sexual Ratio, and Litter Number of the Female Rats. Jentashapir j. health res. 2014;5(5).
  23. Hosseini E, Monfared AL, Moloudizargari M, et al. Histological and morphological characteristics of placenta in the rats administrated with Glycyrrhiza glabra extract. Res. opin. anim. vet. sci. 2013;3(2):60-63.
  24. Ghasemiboroon M, Ghafourian Boroujerdnia M, Ahangarpoor A, et al. The effect of hydro-alcoholic extract of Celery (Apium graveolens) leaves on serum level of testosterone, FSH and LH in male rats. J. of Zanjan University of Med Scie Health Services2013;22(93):49-57. 22038/ijogi.2018.12135.
  25. Kooti W, Ghasemiboroon M, Asadi-Samani M, et al. The effect of halcoholic extract of celery leaves on the delivery rate (fertilization and stillbirths), the number, weight and sex ratio of rat off spring. Adv. Environ. Biol. 2014;8(10):824-830.
  26. Heerwagen MJ, Stewart MS, de la Houssaye BA, Janssen RC, Friedman JEJPo. Transgenic increase in N-3/n-6 Fatty Acid ratio reduces maternal obesity-associated inflammation and limits adverse developmental programming in mice. Ploe One. 2013;8(6):e67791.
  27. Budipitojo T, Shofiyah S, Masithoh D, Khasanah L, Padeta I. The placenta anatomy of sunda porcupine (Hystrix javanica). Adv Anim Vet Sci. 2020;8(3):223-228. 17582/journal.aavs/2020/8.3.223.228
  28. Blanco-Castañeda R, Galaviz-Hernández C, Souto PC, et al. The role of xenobiotic-metabolizing enzymes in the placenta: a growing research field. Expert Rev. Clin. Pharmacol. 2020;13(3):247-263. 1080/17512433.2020.1733412.
  29. Baergen RN. Manual of pathology of the human placenta. Springer Science & Business Media, section IV & V, New york, Springer, 2011.
  30. Hutcheon JA, McNamara H, Platt RW, Benjamin A, Kramer MS. Placental weight for gestational age and adverse perinatal outcomes. Obstet Gynecol. 2012;119(6):1251-1258.
  31. Salafia C, Yampolsky M, Shlakhter A, Mandel D, Schwartz N. Variety in placental shape: when does it originate? Placenta. 2012;33(3):164-170. 1016/j.placenta.2011.12.002.
  32. Hayward CE, Lean S, Sibley CP, et al. Placental adaptation: what can we learn from birthweight: placental weight ratio? Front physiol. 2016;7:28. 3389/fphys.2016.00028.
  33. Herman HG, Miremberg H, Schreiber L, Bar J, Kovo M. The association between disproportionate birth weight to placental weight ratio, clinical outcome, and placental histopathological lesions. Fetal Diagn Ther. 2017;41(4):300-306. 1159/000448949.
  34. Ouyang F, Parker MG, Luo ZC, et al. Maternal BMI, gestational diabetes, and weight gain in relation to childhood obesity: the mediation effect of placental weight. Obesity. 2016;24(4):938-946. 1002/oby.21416.
  35. Ouyang F, Parker M, Cerda S, et al. Placental weight mediates the effects of prenatal factors on fetal growth: the extent differs by preterm status. Obesity. 2013;21(3):609-620. 1002/oby.20254.
  36. Salafia CM, Zhang J, Miller RK, Charles AK, Shrout P, Sun W. Placental growth patterns affect birth weight for given placental weight. Birth Defects Research Part A. clinical and molecular teratology. 2007;79(4):281-288. 1002/bdra.20345.
  37. Alwasel S, Abotalib Z, Aljarallah J, et al. The breadth of the placental surface but not the length is associated with body size at birth. Placenta. 2012;33(8):619-622. 1016/j.placenta.2012.04.015.
  38. Kot K, Kosik-Bogacka D, Łanocha-Arendarczyk N, et al. Interactions between 14 elements in the human placenta, fetal membrane and umbilical cord. Int. J. Environ. Res. Public Health. 2019;16(9):1615. 3390/ijerph16091615.
  39. Barker DJ, Thornburg KL, Osmond C, Kajantie E, Eriksson JG. The surface area of the placenta and hypertension in the offspring in later life. Int. J. Dev. Biol. INT J DEV BIOL. 2010;54:525. 1387/ijdb.082760db.
  40. Schwartz N, Coletta J, Pessel C, et al. Novel 3‐dimensional placental measurements in early pregnancy as predictors of adverse pregnancy outcomes. J Ultrasound Med. 2010;29(8):1203-1212. 7863/jum.2010.29.8.1203.
  41. Eriksson JG, Kajantie E, Osmond C, Thornburg K, Barker DJ. Boys live dangerously in the womb. Am J Hum Biol. 2010;22(3):330-335. 1002/ajhb.20995.
  42. Wong F, Cox BJ. Cellular analysis of trophoblast and placenta. Placenta. 2017;59:S2-S7. 1016/j.placenta.2016.11.015.
  43. Aplin JD, Lewis RM, Jones CJ. Development of the Human Placental Villus. 2018. 1016/B978-0-12-801238-3.99857-X.
  44. Coan P, Conroy N, Burton G, Ferguson‐Smith A. Origin and characteristics of glycogen cells in the developing murine placenta. Developmental dynamics: an official publication of the American Association of Anatomists. Dev. Dyn. 2006;235(12):3280-3294. 1002/dvdy.20981.
  45. Furukawa S, Kuroda Y, Sugiyama A. A comparison of the histological structure of the placenta in experimental animals. J. toxicol. pathol. 2014;27(1):11-18. 1293/tox.2013-0060.
  46. Cross J. How to make a placenta: mechanisms of trophoblast cell differentiation in mice–a review. Placenta. 2005;26:S3-S9. 1016/j.placenta.2005.01.015.
  47. Lefebvre L. The placental imprintome and imprinted gene function in the trophoblast glycogen cell lineage. Reprod. Biomed. Online. 2012;25(1):44-57. 1016/j.rbmo.2012.03.019.
  48. Natale BV, Mehta P, Vu P, et al. Reduced Uteroplacental Perfusion Pressure (RUPP) causes altered trophoblast differentiation and pericyte reduction in the mouse placenta labyrinth. Sci. Rep.
  49. 2018;8(1):1-21. 1038/s41598-018-35606-x.
  50. John R, Hemberger M. A placenta for life. Reprod. Biomed. Online. 2012;25(1):5-11. 1016/j.rbmo.2012.03.018.
  51. Soncin F, Natale D, Parast MM. Signaling pathways in mouse and human trophoblast differentiation: a comparative review. Cellular and Molecular Life Sciences. 2015;72(7):1291-1302.
  52. Hu D, Cross JC. Development and function of trophoblast giant cells in the rodent placenta. Int. J. Dev. Biol. 2009;54(2-3):341-354. 1387/ijdb.082768dh.
  53. Mann C, Hempstock J, Charnock-Jones D, Ferguson-Smith A, Burton G. Oxidative stress and apoptosis in the labyrinth of the normal mouse placenta increase with gestational age. Placenta. 2003;24:A21.
  54. Díaz-Castro J, Florido J, Kajarabille N, et al. A new approach to oxidative stress and inflammatory signaling during labour in healthy mothers and neonates. Oxid. Med. Cell. Longev. 2015; Volume 2015, Article ID 178536, 8 pages.
  55. Ebegboni VJ, Dickenson JM, Sivasubramaniam SD. Antioxidative effects of flavonoids and their metabolites against hypoxia/reoxygenation-induced oxidative stress in a human first trimester trophoblast cell line. Food chem. 2019;272:117-125. 1016/j.foodchem.2018.08.036.
  56. Armant DR. Blastocysts don't go it alone. Extrinsic signals fine-tune the intrinsic developmental program of trophoblast cells. Deve. biol. 2005;280(2):260-280. 1016/j.ydbio.2005.02.009.
  57. Zhang Y, Tan D, Geng Y, et al. Perfluorinated compounds in greenhouse and open agricultural producing areas of three provinces of China: Levels, sources and risk assessment. Int. J. Environ. Res. Public Health. 2016;13(12):1224. 3390/ijerph13121224.
  58. Gorrochategui E, Pérez-Albaladejo E, Casas J, Lacorte S, Porte C. Perfluorinated chemicals: differential toxicity, inhibition of aromatase activity and alteration of cellular lipids in human placental cells. Toxicol. Appl. Pharmacol. 2014;277(2):124-130. 1016/j.taap.2014.03.012.
  59. Hsieh W-T, Huang K-Y, Lin H-Y, Chung J-G. Physalis angulata induced G2/M phase arrest in human breast cancer cells. Food Chem. Toxicol. 2006;44(7):974-983. 1016/j.fct.2005.11.013.
  60. Schindler AE. Progestational effects of dydrogesterone in vitro, in vivo and on the human endometrium. Maturitas. 2009;65:S3-S11. 1016/j.maturitas.2009.10.011.
  61. Costa MA. The endocrine function of human placenta: an overview. Reprod. biomed. online. 2016;32(1):14-43. 1016/j.rbmo.2015.10.005.