Journal of Kerman University of Medical Sciences

Migration of Human Wharton’s Jelly Stem Cells Seeded Onto the Chorioallantoic Membrane Into the Chick Liver

Document Type : Original Article

Authors

Amin Derakhshanfar 1, 2
Javad Moayedi 3
Seyedeh-Sara Hashemi 4
Iman Jamhiri 5
Zeinab Dehghan 1

1 Department of Comparative Biomedical Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

2 Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

3 Central Research Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

4 Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

5 Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

https://doi.org/10.34172/jkmu.3762
Abstract
Background: Developing chicken embryo culture techniques provides methods for manipulating chicken embryos. In this study, the cell-based approach investigated human Wharton›s jelly stem cells (HWJSCs) proliferation and migration into internal embryonic chick tissue.
Methods: Wharton’s jelly cells were cultured on an acellular dermal matrix (ADM) and seeded on the chorioallantoic membrane (CAM) of a 4-day-old chicken embryo. Using an RT-PCR assay with the COLA-I human gene, molecular analysis was performed on chicken liver tissue after hatching to confirm the presence of these cells.
Results: The chicken liver tissue from Wharton’s jelly stem cells/ADM sample tested positive for the human COLA-I gene. The COLA-I gene sequence analysis shows this sequence belongs to the COLA-I gene in humans. The cells cultured on CAM can migrate and proliferate in the liver of a chicken embryo.
Conclusion: These findings suggest that human cells cultured on the CAM can appear in the liver of chickens. However, further investigations are necessary to confirm this finding.

Highlights

Zeinab Dehghan (Google Scholar) (PubMed)

Keywords

Chimeric animal
Human Wharton’s jelly stem cell
Chorioallantoic membrane
Acellular dermal matrix
Chicken embryo
Subjects
  1. Lu Y, Zhou Y, Ju R, Chen J. Human-animal chimeras for autologous organ transplantation: technological advances and future perspectives. Ann Transl Med. 2019;7(20):576. doi: 10.21037/atm.2019.10.13.
  2. Zakeel MC, Marikar FM. Transgenic animal production: present scenario-a review. Bangladesh J Vet Anim Sci. 2017;5(1):1-10. doi: 10.60015/bjvas.vi.34.
  3. Hu M, Wei H, Zhang J, Bai Y, Gao F, Li L, et al. Efficient production of chimeric mice from embryonic stem cells injected into 4- to 8-cell and blastocyst embryos. J Anim Sci Biotechnol. 2013;4(1):12. doi: 10.1186/2049-1891-4-12.
  4. Keefer CL, Baldassarre H, Keyston R, Wang B, Bhatia B, Bilodeau AS, et al. Generation of dwarf goat (Capra hircus) clones following nuclear transfer with transfected and nontransfected fetal fibroblasts and in vitro-matured oocytes. Biol Reprod. 2001;64(3):849-56. doi: 10.1095/ biolreprod64.3.849.
  5. Shakweer WM, Krivoruchko AY, Dessouki SM, Khattab AA. A review of transgenic animal techniques and their applications. J Genet Eng Biotechnol. 2023;21(1):55. doi: 10.1186/s43141- 023-00502-z.
  6. Bednarczyk M, Kozłowska I, Łakota P, Szczerba A, Stadnicka K, Kuwana T. Generation of transgenic chickens by the nonviral, cell-based method: effectiveness of some elements of this strategy. J Appl Genet. 2018;59(1):81-9. doi: 10.1007/ s13353-018-0429-6.
  7. Song G, Park TS, Kim TM, Han JY. Avian biotechnology: insights from germ cell-mediated transgenic systems. J Poult Sci. 2010;47(3):197-207. doi: 10.2141/jpsa.009108.
  8. Wu Z, Yang H, Colosi P. Effect of genome size on AAV vector packaging. Mol Ther. 2010;18(1):80-6. doi: 10.1038/ mt.2009.255.
  9. Li JJ, Lu LZ. Recent progress on technologies and applications of transgenic poultry. Afr J Biotechnol. 2010;9(24):3481-8.
  10. Yi Y, Noh MJ, Lee KH. Current advances in retroviral gene therapy. Curr Gene Ther. 2011;11(3):218-28. doi: 10.2174/156652311795684740.
  11. Chojnacka-Puchta L, Sawicka D, Lakota P, Plucienniczak G, Bednarczyk M, Plucienniczak A. Obtaining chicken primordial germ cells used for gene transfer: in vitro and in vivo results. J Appl Genet. 2015;56(4):493-504. doi: 10.1007/ s13353-015-0276-7.
  12. Baiguera S, Macchiarini P, Ribatti D. Chorioallantoic membrane for in vivo investigation of tissue-engineered construct biocompatibility. J Biomed Mater Res B Appl Biomater. 2012;100(5):1425-34. doi: 10.1002/jbm.b.32653.
  13. Ratner BD, Hoffman AS, Schoen FJ, Lemons JE. Biomaterials Science: An Introduction to Materials in Medicine. Elsevier; 2004.
  14. Bellairs R, Osmond M. Atlas of Chick Development. Elsevier; 2005.
  15. Moreno-Jiménez I, Hulsart-Billstrom G, Lanham SA, Janeczek AA, Kontouli N, Kanczler JM, et al. The chorioallantoic membrane (CAM) assay for the study of human bone regeneration: a refinement animal model for tissue engineering. Sci Rep. 2016;6:32168. doi: 10.1038/srep32168.
  16. Nowak-Sliwinska P, Segura T, Iruela-Arispe ML. The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Angiogenesis. 2014;17(4):779-804. doi: 10.1007/s10456-014-9440-7.
  17. Papoutsi M, Tomarev SI, Eichmann A, Pröls F, Christ B, Wilting J. Endogenous origin of the lymphatics in the avian chorioallantoic membrane. Dev Dyn. 2001;222(2):238-51. doi: 10.1002/dvdy.1187.
  18. Ribatti D. The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The CAM Assay in the Study of Angiogenesis and Metastasis. Springer Science & Business Media; 2010.
  19. Dohle DS, Pasa SD, Gustmann S, Laub M, Wissler JH, Jennissen HP, et al. Chick ex ovo culture and ex ovo CAM assay: how it really works. J Vis Exp. 2009(33):1620. doi: 10.3791/1620.
  20. Huwaikem MA, Kalamegam G, Alrefaei G, Ahmed F, Kadam R, Qadah T, et al. Human Wharton’s jelly stem cell secretions inhibit human leukemic cell line K562 in vitro by inducing cell cycle arrest and apoptosis. Front Cell Dev Biol. 2021;9:614988. doi: 10.3389/fcell.2021.614988.
  21. Rodrigues AZ, de Oliveira PT, Novaes AB Jr, Maia LP, de Souza SL, Palioto DB. Evaluation of in vitro human gingival fibroblast seeding on acellular dermal matrix. Braz Dent J. 2010;21(3):179-89. doi: 10.1590/s0103- 64402010000300001.
  22. Uloza V, Kuzminienė A, Palubinskienė J, Balnytė I, Ulozienė I, Valančiūtė A. Model of human recurrent respiratory papilloma on chicken embryo chorioallantoic membrane for tumor angiogenesis research. Histol Histopathol. 2017;32(7):699- 710. doi: 10.14670/hh-11-831.
  23. Derakhshanfar A, Moayedi J, Vahedi M, Valizadeh A. Arum conophalloides aqueous extract induced hepatotoxicity in rat; histopathological, biochemical, and miR-122 assessments. Microrna. 2020;9(3):224-31. doi: 10.2174/22115366086661 91016142400.
  24. Ribatti D. The chick embryo chorioallantoic membrane (CAM). A multifaceted experimental model. Mech Dev. 2016;141:70-7. doi: 10.1016/j.mod.2016.05.003.
  25. Martinez-Madrid B, Donnez J, Van Eyck AS, Veiga-Lopez A, Dolmans MM, Van Langendonckt A. Chick embryo chorioallantoic membrane (CAM) model: a useful tool to study short-term transplantation of cryopreserved human ovarian tissue. Fertil Steril. 2009;91(1):285-92. doi: 10.1016/j.fertnstert.2007.11.026.
  26. Maeda Y, Noda M. Coordinated development of embryonic long bone on chorioallantoic membrane in ovo prevents perichondrium-derived suppressive signals against cartilage growth. Bone. 2003;32(1):27-34. doi: 10.1016/s8756- 3282(02)00917-1.
  27. Noiman T, Buzhor E, Metsuyanim S, Harari-Steinberg O, Morgenshtern C, Dekel B, et al. A rapid in vivo assay system for analyzing the organogenetic capacity of human kidney cells. Organogenesis. 2011;7(2):140-4. doi: 10.4161/ org.7.2.16457.
  28. Mozdziak PE, Wysocki R, Angerman-Stewart J, Pardue SL, Petitte JN. Production of chick germline chimeras from fluorescence-activated cell-sorted gonocytes. Poult Sci. 2006;85(10):1764-8. doi: 10.1093/ps/85.10.1764.
  29. Jiang ZQ, Wu HY, Tian J, Li N, Hu XX. Targeting lentiviral vectors to primordial germ cells (PGCs): an efficient strategy for generating transgenic chickens. Zool Res. 2020;41(3):281- 91. doi: 10.24272/j.issn.2095-8137.2020.032.
  30. Boháč M, Danišovič Ľ, Koller J, Dragúňová J, Varga I. What happens to an acellular dermal matrix after implantation in the human body? A histological and electron microscopic study. Eur J Histochem. 2018;62(1):2873. doi: 10.4081/ ejh.2018.2873.
  31. Conconi MT, Nico B, Mangieri D, Tommasini M, di Liddo R, Parnigotto PP, et al. Angiogenic response induced by acellular aortic matrix in vivo. Anat Rec A Discov Mol Cell Evol Biol. 2004;281(2):1303-7. doi: 10.1002/ar.a.20137.
  32. Conconi MT, Nico B, Rebuffat P, Crivellato E, Parnigotto PP, Nussdorfer GG, et al. Angiogenic response induced by acellular femoral matrix in vivo. J Anat. 2005;207(1):79-83. doi: 10.1111/j.1469-7580.2005.00427.x.
  33. Conconi MT, Bellini S, Teoli D, de Coppi P, Ribatti D, Nico B, et al. In vitro and in vivo evaluation of acellular diaphragmatic matrices seeded with muscle precursors cells and coated with VEGF silica gels to repair muscle defect of the diaphragm. J Biomed Mater Res A. 2009;89(2):304-16. doi: 10.1002/ jbm.a.31982.
  34. Marzaro M, Vigolo S, Oselladore B, Conconi MT, Ribatti D, Giuliani S, et al. In vitro and in vivo proposal of an artificial esophagus. J Biomed Mater Res A. 2006;77(4):795-801. doi: 10.1002/jbm.a.30666.
  35. Ribatti D, Conconi MT, Nico B, Baiguera S, Corsi P, Parnigotto PP, et al. Angiogenic response induced by acellular brain scaffolds grafted onto the chick embryo chorioallantoic membrane. Brain Res. 2003;989(1):9-15. doi: 10.1016/s0006- 8993(03)03225-6.
  36. Hen G, Yosefi S, Shinder D, Or A, Mygdal S, Condiotti R, et al. Gene transfer to chicks using lentiviral vectors administered via the embryonic chorioallantoic membrane. PLoS One. 2012;7(5):e36531. doi: 10.1371/journal.pone.0036531.
  37. Naito M, Sakurai M, Kuwana T. Expression of exogenous DNA in the gonads of chimaeric chicken embryos produced by transfer of primordial germ cell transfected in vitro and subsequent fate of the introduced DNA. J Reprod Fertil. 1998;113(1):137-43. doi: 10.1530/jrf.0.1130137.
  38. Park TS, Han JY. piggyBac transposition into primordial germ cells is an efficient tool for transgenesis in chickens. Proc Natl Acad Sci U S A. 2012;109(24):9337-41. doi: 10.1073/ pnas.1203823109.
Journal of Kerman University of Medical Sciences
Volume 32
Pages 1-7

Home

Editorial Board

Guide for Authors

Submit Manuscript

Guide for Reviewers

Contact Us