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Migration of Human Wharton’s Jelly Stem Cells Seeded Onto the Chorioallantoic Membrane Into the Chick Liver

Document Type : Original Article

Authors

1 Department of Comparative Biomedical Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

2 Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

3 Central Research Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

4 Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

5 Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Background: Developing chicken embryo culture techniques provides methods for manipulating chicken embryos. In this study, the cell-based approach investigated human Wharton›s jelly stem cells (HWJSCs) proliferation and migration into internal embryonic chick tissue.
Methods: Wharton’s jelly cells were cultured on an acellular dermal matrix (ADM) and seeded on the chorioallantoic membrane (CAM) of a 4-day-old chicken embryo. Using an RT-PCR assay with the COLA-I human gene, molecular analysis was performed on chicken liver tissue after hatching to confirm the presence of these cells.
Results: The chicken liver tissue from Wharton’s jelly stem cells/ADM sample tested positive for the human COLA-I gene. The COLA-I gene sequence analysis shows this sequence belongs to the COLA-I gene in humans. The cells cultured on CAM can migrate and proliferate in the liver of a chicken embryo.
Conclusion: These findings suggest that human cells cultured on the CAM can appear in the liver of chickens. However, further investigations are necessary to confirm this finding.

Keywords

Main Subjects

References
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Journal of Kerman University of Medical Sciences
Volume 32
Pages 1-7
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