Journal of Kerman University of Medical Sciences

Dual Effects of Aspirin on Spatial Learning and Memory of Male Rats Following Induction of Permanent Cerebral Ischemia

Document Type : Original Article

Authors

Narges Ashraf Ganjooei 1
Vahid Sheibani 1
Mandana Jafari 2
Zahra Hajializadeh 3
Ali Shamsara 4
Ali Dehghani 5
Reza Afarinesh 1

1 Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

2 Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran

3 Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

4 Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

5 Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

https://doi.org/10.34172/jkmu.3837
Abstract
Background: Ischemic stroke can lead to cognitive impairment, and nonsteroidal anti-inflammatory drugs (NSAIDs) have been shown to slow down the progression of Alzheimer›s disease (AD). This study focused on rodent models to investigate the impact of ischemic stroke and the potential benefits of aspirin in reducing cognitive impairment.
Methods: The Morris water maze (MWM) was used to evaluate memory and learning in seven groups (N=63) of Wistar rats. Brain ischemia was induced in rat models through temporary blocking of both common carotid arteries and permanent blocking of the middle cerebral artery (MCA). Aspirin 20, 40, and 80 mg/kg IP was administered 30 minutes and 2 hours after stroke induction in both ischemic and non-ischemic rats. Injections were continued for seven consecutive days in these groups, and learning and memory were evaluated after the last injection.
Results: Data analysis of the MWM test showed a significant increase in escape latency and swim path length to find the platform in the ischemic groups compared to control rats (P<0.005). Despite improvement in all experimental groups after intervention (P<0.001), the scores for spatial learning were significantly decreased by aspirin in no-ischemia+ASA groups compared to the control group (P<0.05). In the ischemia+ASA groups, aspirin at the dose of 20 mg/kg but not at the high dose (80 mg/kg) improved spatial learning compared to the control group.
Conclusion: Repeated treatments with aspirin may impair spatial learning and memory in normal rats, however, aspirin at a low dose of 20 mg/kg may improve learning impairment after ischemic stroke.

Highlights

Reza Afarinesh (Google Scholar) (PubMed)

Keywords

Aspirin
Cerebral Ischemia
Learning
Memory
Rats
Stroke
Subjects
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Journal of Kerman University of Medical Sciences
Volume 32
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